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Gene information
General information
TC003627 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9539357..9547101
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003627
    iBeetle screen iB_03592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003627 RA
      MAEAIFSVL KEGLSLSQA ARKYDIPYP TFVLYANRV 
      HNMLGPSAD GGSDLRPKG RGRPQRILL GVWPDEHIR 
      GVIRAVVFR DSHQIKDEP HVAYPRIHD GVSIHSYPN 
      NSCSNGSEA NVSPGAAAA AAVAAVAQG LRQQMCSMV 
      AAAQHQSSA HDTNPVASL VNSLALAGH CGNVSMSSN 
      GAAAPMGPL RSMSSPAGS IQDLRISPA ESALESPIS 
      SPLGLTVSS SLEPAINLN IGNSMDVGM GMTYKPTRA 
      FASPRPENL FQEDIDELV KPMSTPRPK DAMATIKME 
      PMAECRGE
    CDS sequence
    • >TC003627 RA
      atggccgag gcaatcttc tcggtgctt aaggagggc 
      ttatccctg tcccaggcc gccaggaaa tacgatatt 
      ccctatccc acgtttgtt ctgtacgcg aacagagtg 
      cacaacatg ctaggaccg tccgcggac ggcggatca 
      gatttacgg ccgaaagga cgagggcgg ccccaaaga 
      attctgttg ggggtttgg cccgatgag catatacgg 
      ggtgttatc agggccgta gtgttcaga gactcgcat 
      caaatcaaa gacgaacca cacgtcgca tacccacga 
      attcatgac ggcgtcagc atacacagc tacccgaac 
      aactcgtgc agcaacggt tccgaagcg aacgtttcc 
      ccaggtgcg gcggcggcg gcggccgtg gcggcggtg 
      gcccagggc ctccgccag cagatgtgc agcatggtg 
      gcggcggcg cagcaccag agctccgct cacgacacc 
      aaccccgtg gccagcctg gtgaactcg ctggcgttg 
      gccggccac tgcggcaac gtgtccatg tccagcaac 
      ggcgccgcc gcccccatg ggccccctg cgcagcatg 
      agcagcccc gccggcagc atccaggac ctgcgcatc 
      agccccgcc gagtcggcc ctcgagagc cccatctcg 
      tcgccgctg gggctcacc gtctccagc tcgctggag 
      cccgccatc aacctcaac atcggcaac agcatggac 
      gtgggcatg ggcatgacc tacaagccc accagggcc 
      ttcgcctcc cccaggccc gagaacctc ttccaggag 
      gacatcgac gagctggtc aagcccatg tccacgccc 
      aggcccaag gacgccatg gccaccatc aagatggag 
      cccatggcg gagtgtcgc ggcgaatga
    mRNA sequence
    • >TC003627 RA
      cgcgtaaaa tcaccgaat attcctgct gcgctcgca 
      ggaattcaa acttggagt agtaagcac tccacccta 
      aatagaaaa ctaattagg taattgcac aattaaaaa 
      agtttgaat tgatctcga ccatttgta ataaatttt 
      atgttacag gtacccttc aagctggta atttttttc 
      actttattt ttattttat tgttgaact tatcgtgca 
      ttaaattgt acgactcgc taatagtca ccagttttt 
      ttcacttaa tccctagcg agtcgtcca atttggcca 
      taatttttc atgtgctga gtttatcag tgtgtcgat 
      cggcatact aatgtggat atcgttttc tttttcttt 
      cagcagaca gctaccagt accagttgc aatccatgt 
      ggcagaaat gttggaata cgaatcaaa gtcttgtac 
      acaacttgc gcttccgtg aacgagggc cgctgaaat 
      cctggcgac ctgaaacca tggccgagg caatcttct 
      cggtgctta aggagggct tatccctgt cccaggccg 
      ccaggaaat acgatattc cctatccca cgtttgttc 
      tgtacgcga acagagtgc acaacatgc taggaccgt 
      ccgcggacg gcggatcag atttacggc cgaaaggac 
      gagggcggc cccaaagaa ttctgttgg gggtttggc 
      ccgatgagc atatacggg gtgttatca gggccgtag 
      tgttcagag actcgcatc aaatcaaag acgaaccac 
      acgtcgcat acccacgaa ttcatgacg gcgtcagca 
      tacacagct acccgaaca actcgtgca gcaacggtt 
      ccgaagcga acgtttccc caggtgcgg cggcggcgg 
      cggccgtgg cggcggtgg cccagggcc tccgccagc 
      agatgtgca gcatggtgg cggcggcgc agcaccaga 
      gctccgctc acgacacca accccgtgg ccagcctgg 
      tgaactcgc tggcgttgg ccggccact gcggcaacg 
      tgtccatgt ccagcaacg gcgccgccg cccccatgg 
      gccccctgc gcagcatga gcagccccg ccggcagca 
      tccaggacc tgcgcatca gccccgccg agtcggccc 
      tcgagagcc ccatctcgt cgccgctgg ggctcaccg 
      tctccagct cgctggagc ccgccatca acctcaaca 
      tcggcaaca gcatggacg tgggcatgg gcatgacct 
      acaagccca ccagggcct tcgcctccc ccaggcccg 
      agaacctct tccaggagg acatcgacg agctggtca 
      agcccatgt ccacgccca ggcccaagg acgccatgg 
      ccaccatca agatggagc ccatggcgg agtgtcgcg 
      gcgaatgaa acgagacaa tgctacaat gtaatgtga 
      taaattatt attaggttt attttctat tatcaatta 
      actgttaat tatttctaa agttttagt gggtagaat 
      gttttcgac gattttata aaatttatc tagaagcca 
      aagtttcgg ccagttcca taaaagact gttgtgtat 
      atgtatact attcgaaat tcaaaatta gagtggata 
      ttttctgta gggaaatca cattagcag ggaaactat 
      atcaacatt cccacattc gctttacga caatcggtt 
      gatgaagga agaattcta ttcaatttt catatcgcg 
      gattttttt cggtttttg acactgact tatcggggc 
      ccaaactac tcaaaagtt atgcaataa tatgatatt 
      ctaaaattt gaaattcca ggcacctgt acacatata 
      caaatttat gattaaaat tatttccag cctttgtaa 
      aatgtttat ttttattta tgtcgcaaa cagaggcca 
      tggactaaa actgcgggc aaaacggtt tttttttct 
      ccaaaatac tcgaataac tgaggctta aattctcaa 
      tgaggtgag aaaaactgc ccacgaaat tttcaaaac 
      ttttaaaga tgcaaaaaa tgtgaaagg aacaaaact 
      gttttactt caattttta cttcaaaca atctcttag 
      tttaggctc taaagatgt taaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY