General information | |||
TC031038
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 9318922..9537293 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031038 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03591 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
head capsule complete shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs partially sometimesbent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
prothorax segment irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: prothorax appears to be shiftet towards anterior and fused with the head |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 5 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
- >TC031038 RA
MKKLAVGWE PVISNKWQN RRKAWRLFE RNQQVAGAA TAAPLFTTL AAPRRVPPP YPPVFFAHL PSNAPLMGQ NVWILFGFG LFSLLCVDV PLCFLLLSS SRFSYHPFS LTHSKSLQV TTINMQHTS GSPQQFCLR WNNYQSNLT NVFDQLLQS ESFVDVTLA CDGHSVKAH KMVLSACSP YFQSLFFEN PCQHPIVIM RDIKWPELK AAVEFMYKG EINVSQEQI GPLLKVAES LKIRGLADV NGEQDIVAP TGELTTSTR TLLKTASTP NNGSGEWEC RPEGDGPPR VKKRRRPSG ERSSLSSPA ESASTEIPD PPPSVEMSP APSATPLPS SVSNEPLPL TLPLPQQPQ VSHASSDDM EIKPGIAEM IREEERAKL LESSHAWLG ASTSSIADY PTLLKLKTP TWTQEQLKE AIDAVVKQK MRFTQASAR YGIPKGTLY DNILGKSKR MAVLEEAGL TPSEEAAVL EFCCDVSVS PYNRRTKKS LQSVLNFVE KLRRARDPE FIFTGLSGF RWWWAFCKK HNIVSLYYE NNGSMHSLN NTL
- >TC031038 RA
atgaaaaaa ttagcggtt ggttgggag ccggtcatt agcaacaag tggcaaaat cgccgaaaa gcatggcgt ctgtttgaa cggaatcaa caagtcgcg ggcgccgcg acggcagcg ccgctattt acgacgttg gccgcgcca cgacgcgtc cctccacca tatccacct gttttcttt gcacaccta ccatcaaat gcacctttg atgggtcaa aatgtttgg attttgttt ggttttggc ttgttttcg ttgttgtgt gtagacgtg ccgctttgt tttcttttg ttgagttcg agccgtttt agctatcat ccgttttcg ttgacgcat tcgaagtcg ttgcaggtg accaccatc aacatgcag cacacctcg ggctccccc cagcagttc tgcttgcgc tggaacaac taccaatcc aacctgacc aacgtcttc gaccagctc ctccagagc gagtcgttc gtggacgtc accctcgcc tgcgatggc cactccgtc aaggcccac aagatggtc ctctcggcc tgcagcccc tatttccag tcactcttt ttcgagaac ccctgccag cacccgatc gtcatcatg cgggacatc aagtggccc gaactcaaa gccgccgtt gagttcatg tacaagggc gagatcaac gtctcgcag gagcagatc gggcccttg ctcaaagtc gccgaaagc ttgaaaatc cgcggcttg gccgacgtc aacggcgag caagacatc gtcgccccg accggcgag ctcaccacc agcaccagg accctcctg aagacggcc tccaccccg aacaacggc tccggcgag tgggaatgc cggccggag ggtgacggc ccgccacgt gtcaaaaag agacgcaga ccttccgga gaacgcagc agcctgagc agtccggct gagagtgcg agcacggaa attccggat cctccgcct tcggtcgaa atgtcaccg gcgccgtcc gcgacgccg cttccttct tccgtttcg aacgaaccc ttgccgcta acgttaccg cttccgcaa caaccgcaa gtatcacac gcgtcctcg gacgatatg gaaatcaaa ccaggcatt gctgagatg atacgcgag gaggaacgg gccaagttg ttggagtct tcccatgct tggctcggc gcctctacg tcttctata gccgattat ccgacatta ctaaaactt aaaaccccc acatggact caagagcag ctcaaagaa gcaatcgat gccgtcgtg aagcaaaaa atgcgcttc acgcaggct tcggccagg tatggtata ccaaagggg acgttgtac gataatatc ctagggaaa tcgaagcgg atggccgtg ctggaggag gccgggctg accccctcc gaggaggcg gcggtgctg gagttttgt tgcgatgtc tcagtttcg ccgtataac agacgcacg aaaaaatcg ctgcagtcg gtgctcaac tttgtggag aaactgaga cgcgcccga gacccggag ttcatcttc accggactt tccgggttc cggtggtgg tgggcgttt tgcaaaaag cacaatatt gtctcgctt tattacgag aacaacggt tctatgcat tccttaaat aatactctt tga
- >TC031038 RA
ttgtttaca taagttaat aaacacatg ttagatctg tcaaacgcc acgtgcttc ttgtataca caagtgttt tatgaaaaa attagcggt tggttggga gccggtcat tagcaacaa gtggcaaaa tcgccgaaa agcatggcg tctgtttga acggaatca acaagtcgc gggcgccgc gacggcagc gccgctatt tacgacgtt ggccgcgcc acgacgcgt ccctccacc atatccacc tgttttctt tgcacacct accatcaaa tgcaccttt gatgggtca aaatgtttg gattttgtt tggttttgg cttgttttc gttgttgtg tgtagacgt gccgctttg ttttctttt gttgagttc gagccgttt tagctatca tccgttttc gttgacgca ttcgaagtc gttgcaggt gaccaccat caacatgca gcacacctc gggctcccc ccagcagtt ctgcttgcg ctggaacaa ctaccaatc caacctgac caacgtctt cgaccagct cctccagag cgagtcgtt cgtggacgt caccctcgc ctgcgatgg ccactccgt caaggccca caagatggt cctctcggc ctgcagccc ctatttcca gtcactctt tttcgagaa cccctgcca gcacccgat cgtcatcat gcgggacat caagtggcc cgaactcaa agccgccgt tgagttcat gtacaaggg cgagatcaa cgtctcgca ggagcagat cgggccctt gctcaaagt cgccgaaag cttgaaaat ccgcggctt ggccgacgt caacggcga gcaagacat cgtcgcccc gaccggcga gctcaccac cagcaccag gaccctcct gaagacggc ctccacccc gaacaacgg ctccggcga gtgggaatg ccggccgga gggtgacgg cccgccacg tgtcaaaaa gagacgcag accttccgg agaacgcag cagcctgag cagtccggc tgagagtgc gagcacgga aattccgga tcctccgcc ttcggtcga aatgtcacc ggcgccgtc cgcgacgcc gcttccttc ttccgtttc gaacgaacc cttgccgct aacgttacc gcttccgca acaaccgca agtatcaca cgcgtcctc ggacgatat ggaaatcaa accaggcat tgctgagat gatacgcga ggaggaacg ggccaagtt gttggagtc ttcccatgc ttggctcgg cgcctctac gtcttctat agccgatta tccgacatt actaaaact taaaacccc cacatggac tcaagagca gctcaaaga agcaatcga tgccgtcgt gaagcaaaa aatgcgctt cacgcaggc ttcggccag gtatggtat accaaaggg gacgttgta cgataatat cctagggaa atcgaagcg gatggccgt gctggagga ggccgggct gaccccctc cgaggaggc ggcggtgct ggagttttg ttgcgatgt ctcagtttc gccgtataa cagacgcac gaaaaaatc gctgcagtc ggtgctcaa ctttgtgga gaaactgag acgcgcccg agacccgga gttcatctt caccggact ttccgggtt ccggtggtg gtgggcgtt ttgcaaaaa gcacaatat tgtctcgct ttattacga gaacaacgg ttctatgca ttccttaaa taatactct ttgaagacg atgtgatca acgtttagc aataaattc taaaactgt taattgggg caatatttt tctgtatat aggagttac gttaatgcc tgatgttta cagaattcg agttgattt atgaacttt tagatttta taaagttac gagtaggga acttgaatt ttacattcc ttagcttct cattgtact tccttccat tcctatatt attatcatt attttttta cttgttagc tttatttgt acatattgc atcctttat taacgttac tttgttatt aataatacg atgtttgcg ttcaagtga gctatattt atgcaacta attacatgg ctattatac catgcgtgt tatattatt atattcaac atgcgatat tgtgtttca ttattagtt tagttatat tttattttt attaataac ttattttct cacgtattt aagtcggac acatgcatc gatcattat caatttagt tacattttg tagccgatg gcagctttt taatcacat ggtgcctta actacttga taatgattt ttagtttta gtttatttt tagcaataa ttttttttt agaataatt tctcacgtc gttatgtac gacgatatt tttgagacg atgttactt tcttcaaat tattttgca aattatact cctaataaa caattatta actat
