Status
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Gene information
General information
TC031038
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003621,TC003626
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9318922..9537293
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031038
    iBeetle screen iB_03591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule complete shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs partially sometimesbent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    prothorax segment irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: prothorax appears to be shiftet towards anterior and fused with the head
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 7

    Adult morphology
    wings shortened - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031038 RA
      MKKLAVGWE PVISNKWQN RRKAWRLFE RNQQVAGAA 
      TAAPLFTTL AAPRRVPPP YPPVFFAHL PSNAPLMGQ 
      NVWILFGFG LFSLLCVDV PLCFLLLSS SRFSYHPFS 
      LTHSKSLQV TTINMQHTS GSPQQFCLR WNNYQSNLT 
      NVFDQLLQS ESFVDVTLA CDGHSVKAH KMVLSACSP 
      YFQSLFFEN PCQHPIVIM RDIKWPELK AAVEFMYKG 
      EINVSQEQI GPLLKVAES LKIRGLADV NGEQDIVAP 
      TGELTTSTR TLLKTASTP NNGSGEWEC RPEGDGPPR 
      VKKRRRPSG ERSSLSSPA ESASTEIPD PPPSVEMSP 
      APSATPLPS SVSNEPLPL TLPLPQQPQ VSHASSDDM 
      EIKPGIAEM IREEERAKL LESSHAWLG ASTSSIADY 
      PTLLKLKTP TWTQEQLKE AIDAVVKQK MRFTQASAR 
      YGIPKGTLY DNILGKSKR MAVLEEAGL TPSEEAAVL 
      EFCCDVSVS PYNRRTKKS LQSVLNFVE KLRRARDPE 
      FIFTGLSGF RWWWAFCKK HNIVSLYYE NNGSMHSLN 
      NTL
    CDS sequence
    • >TC031038 RA
      atgaaaaaa ttagcggtt ggttgggag ccggtcatt 
      agcaacaag tggcaaaat cgccgaaaa gcatggcgt 
      ctgtttgaa cggaatcaa caagtcgcg ggcgccgcg 
      acggcagcg ccgctattt acgacgttg gccgcgcca 
      cgacgcgtc cctccacca tatccacct gttttcttt 
      gcacaccta ccatcaaat gcacctttg atgggtcaa 
      aatgtttgg attttgttt ggttttggc ttgttttcg 
      ttgttgtgt gtagacgtg ccgctttgt tttcttttg 
      ttgagttcg agccgtttt agctatcat ccgttttcg 
      ttgacgcat tcgaagtcg ttgcaggtg accaccatc 
      aacatgcag cacacctcg ggctccccc cagcagttc 
      tgcttgcgc tggaacaac taccaatcc aacctgacc 
      aacgtcttc gaccagctc ctccagagc gagtcgttc 
      gtggacgtc accctcgcc tgcgatggc cactccgtc 
      aaggcccac aagatggtc ctctcggcc tgcagcccc 
      tatttccag tcactcttt ttcgagaac ccctgccag 
      cacccgatc gtcatcatg cgggacatc aagtggccc 
      gaactcaaa gccgccgtt gagttcatg tacaagggc 
      gagatcaac gtctcgcag gagcagatc gggcccttg 
      ctcaaagtc gccgaaagc ttgaaaatc cgcggcttg 
      gccgacgtc aacggcgag caagacatc gtcgccccg 
      accggcgag ctcaccacc agcaccagg accctcctg 
      aagacggcc tccaccccg aacaacggc tccggcgag 
      tgggaatgc cggccggag ggtgacggc ccgccacgt 
      gtcaaaaag agacgcaga ccttccgga gaacgcagc 
      agcctgagc agtccggct gagagtgcg agcacggaa 
      attccggat cctccgcct tcggtcgaa atgtcaccg 
      gcgccgtcc gcgacgccg cttccttct tccgtttcg 
      aacgaaccc ttgccgcta acgttaccg cttccgcaa 
      caaccgcaa gtatcacac gcgtcctcg gacgatatg 
      gaaatcaaa ccaggcatt gctgagatg atacgcgag 
      gaggaacgg gccaagttg ttggagtct tcccatgct 
      tggctcggc gcctctacg tcttctata gccgattat 
      ccgacatta ctaaaactt aaaaccccc acatggact 
      caagagcag ctcaaagaa gcaatcgat gccgtcgtg 
      aagcaaaaa atgcgcttc acgcaggct tcggccagg 
      tatggtata ccaaagggg acgttgtac gataatatc 
      ctagggaaa tcgaagcgg atggccgtg ctggaggag 
      gccgggctg accccctcc gaggaggcg gcggtgctg 
      gagttttgt tgcgatgtc tcagtttcg ccgtataac 
      agacgcacg aaaaaatcg ctgcagtcg gtgctcaac 
      tttgtggag aaactgaga cgcgcccga gacccggag 
      ttcatcttc accggactt tccgggttc cggtggtgg 
      tgggcgttt tgcaaaaag cacaatatt gtctcgctt 
      tattacgag aacaacggt tctatgcat tccttaaat 
      aatactctt tga
    mRNA sequence
    • >TC031038 RA
      ttgtttaca taagttaat aaacacatg ttagatctg 
      tcaaacgcc acgtgcttc ttgtataca caagtgttt 
      tatgaaaaa attagcggt tggttggga gccggtcat 
      tagcaacaa gtggcaaaa tcgccgaaa agcatggcg 
      tctgtttga acggaatca acaagtcgc gggcgccgc 
      gacggcagc gccgctatt tacgacgtt ggccgcgcc 
      acgacgcgt ccctccacc atatccacc tgttttctt 
      tgcacacct accatcaaa tgcaccttt gatgggtca 
      aaatgtttg gattttgtt tggttttgg cttgttttc 
      gttgttgtg tgtagacgt gccgctttg ttttctttt 
      gttgagttc gagccgttt tagctatca tccgttttc 
      gttgacgca ttcgaagtc gttgcaggt gaccaccat 
      caacatgca gcacacctc gggctcccc ccagcagtt 
      ctgcttgcg ctggaacaa ctaccaatc caacctgac 
      caacgtctt cgaccagct cctccagag cgagtcgtt 
      cgtggacgt caccctcgc ctgcgatgg ccactccgt 
      caaggccca caagatggt cctctcggc ctgcagccc 
      ctatttcca gtcactctt tttcgagaa cccctgcca 
      gcacccgat cgtcatcat gcgggacat caagtggcc 
      cgaactcaa agccgccgt tgagttcat gtacaaggg 
      cgagatcaa cgtctcgca ggagcagat cgggccctt 
      gctcaaagt cgccgaaag cttgaaaat ccgcggctt 
      ggccgacgt caacggcga gcaagacat cgtcgcccc 
      gaccggcga gctcaccac cagcaccag gaccctcct 
      gaagacggc ctccacccc gaacaacgg ctccggcga 
      gtgggaatg ccggccgga gggtgacgg cccgccacg 
      tgtcaaaaa gagacgcag accttccgg agaacgcag 
      cagcctgag cagtccggc tgagagtgc gagcacgga 
      aattccgga tcctccgcc ttcggtcga aatgtcacc 
      ggcgccgtc cgcgacgcc gcttccttc ttccgtttc 
      gaacgaacc cttgccgct aacgttacc gcttccgca 
      acaaccgca agtatcaca cgcgtcctc ggacgatat 
      ggaaatcaa accaggcat tgctgagat gatacgcga 
      ggaggaacg ggccaagtt gttggagtc ttcccatgc 
      ttggctcgg cgcctctac gtcttctat agccgatta 
      tccgacatt actaaaact taaaacccc cacatggac 
      tcaagagca gctcaaaga agcaatcga tgccgtcgt 
      gaagcaaaa aatgcgctt cacgcaggc ttcggccag 
      gtatggtat accaaaggg gacgttgta cgataatat 
      cctagggaa atcgaagcg gatggccgt gctggagga 
      ggccgggct gaccccctc cgaggaggc ggcggtgct 
      ggagttttg ttgcgatgt ctcagtttc gccgtataa 
      cagacgcac gaaaaaatc gctgcagtc ggtgctcaa 
      ctttgtgga gaaactgag acgcgcccg agacccgga 
      gttcatctt caccggact ttccgggtt ccggtggtg 
      gtgggcgtt ttgcaaaaa gcacaatat tgtctcgct 
      ttattacga gaacaacgg ttctatgca ttccttaaa 
      taatactct ttgaagacg atgtgatca acgtttagc 
      aataaattc taaaactgt taattgggg caatatttt 
      tctgtatat aggagttac gttaatgcc tgatgttta 
      cagaattcg agttgattt atgaacttt tagatttta 
      taaagttac gagtaggga acttgaatt ttacattcc 
      ttagcttct cattgtact tccttccat tcctatatt 
      attatcatt attttttta cttgttagc tttatttgt 
      acatattgc atcctttat taacgttac tttgttatt 
      aataatacg atgtttgcg ttcaagtga gctatattt 
      atgcaacta attacatgg ctattatac catgcgtgt 
      tatattatt atattcaac atgcgatat tgtgtttca 
      ttattagtt tagttatat tttattttt attaataac 
      ttattttct cacgtattt aagtcggac acatgcatc 
      gatcattat caatttagt tacattttg tagccgatg 
      gcagctttt taatcacat ggtgcctta actacttga 
      taatgattt ttagtttta gtttatttt tagcaataa 
      ttttttttt agaataatt tctcacgtc gttatgtac 
      gacgatatt tttgagacg atgttactt tcttcaaat 
      tattttgca aattatact cctaataaa caattatta 
      actat
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