Status
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Gene information
General information
TC034593 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8624429..8634721
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034593
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03589

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034593 RA
      MDSSSSSSI EKCNCDKSE PSQESDSSN SECSPKDKK 
      IKISLTSLG LNLRRGRSK RNKKTNQIS PSTSSGAGT 
      SKEITKKSP KWGIKFNCA KKEPKVAFV EENTNCCKC 
      TCYRRTEEH EAGPGTVFA SEDNERTNE VVEHSNEEE 
      NREEVQNES EEGEGVKGI YGISRNSSC LCIGPWDMH 
      WIRTVHEDC DEAARLARA REIEQGVDP PANFRPVRR 
      VQVLCSDMD SEGGQVPRR LQLVCPPDL TVDTLRALF 
      QNHVTLRSC PLDTIAGCH TQVDFIHCL VPDLLEITK 
      CSFYWGKMD RYEAERLLD GKPEGTFLL RDSAQEEFL 
      FSVSFRKYG RSLHARIEQ WNHKFSFDS HDPGVYTSD 
      TVCGLIEHY KDPSSCMFF EPMLTWPLH RNFTFSLQH 
      LCRATIVNR LTYDNINLL QLPKTLKSY LKEYHYRQK 
      VRVERFDDD MQWLDLRKV AS
    CDS sequence
    • >TC034593 RA
      atggactca agtagcagt tccagtatc gagaaatgc 
      aactgcgac aagtcggaa ccatcgcaa gaaagcgac 
      agtagtaat tcggaatgt tcccccaaa gataagaaa 
      atcaaaatc agtctgact tcgctcggt ctgaatttg 
      cgcagaggt cgttcgaaa cgtaacaag aaaacgaat 
      caaatctca ccgtccact tcttcgggt gctggcacc 
      agtaaggaa atcacgaag aagtcgccg aaatggggc 
      atcaaattc aattgtgct aagaaagaa ccgaaagtg 
      gccttcgtg gaagagaac acgaattgt tgcaagtgc 
      acgtgttac agaaggacg gaggagcac gaagcaggg 
      cctgggaca gtgtttgcg agtgaggat aatgaaagg 
      acgaatgag gtggtagag cattcgaac gaggaggaa 
      aatagggaa gaggtgcag aatgagagt gaggaaggc 
      gaaggggtt aaaggcatt tatggcatc tcgaggaat 
      tcgagctgc ttgtgcatt ggtccctgg gacatgcac 
      tggatacgt actgtacac gaagactgt gatgaagcc 
      gctcgatta gcgcgtgct cgcgaaatc gagcaagga 
      gttgatcct ccagcaaat tttcgcccc gtacgaagg 
      gtgcaagtg ctctgctcc gacatggac tcagaaggc 
      ggccaagtg ccgcggagg ctgcagctg gtctgccct 
      ccagaccta accgtagac accctgcgg gcccttttc 
      caaaaccac gtgactctc cggtcctgt cctttggac 
      accatcgcc gggtgccac actcaggtt gacttcatt 
      cattgtctc gtacctgat ctgctggaa atcaccaaa 
      tgcagtttt tactggggt aaaatggac cgctacgag 
      gcggagagg ctgctcgac ggtaagccc gaaggcact 
      ttcctcctc cgcgactcc gcccaggaa gagttcctt 
      ttcagcgtg tctttccgc aagtacggc cggtcgttg 
      catgcgaga atagaacag tggaatcat aagttcagt 
      ttcgattcg cacgacccc ggcgtctac acgtctgac 
      acagtttgt ggccttatc gaacattac aaagacccg 
      agtagttgc atgttcttc gagccgatg ctcacatgg 
      ccgttgcac aggaacttt acgttttcg ctgcaacat 
      ttgtgcaga gcgacgatc gttaatcgg ttgacttac 
      gataatatt aatctgttg caattgcct aagacgttg 
      aaaagttat ctcaaagag taccattat agacagaaa 
      gtgagagtg gagaggttt gacgacgac atgcagtgg 
      ctggatttg aggaaagta gcttcgtaa
    mRNA sequence
    • >TC034593 RA
      gctgccatg tcacttttc ctcctattt atcaaaaaa 
      ttttaaacg ggttgtgca aacaagcga gttatcgaa 
      gaatagaat tggttcaca aaaagtaaa cataaatag 
      ttgtgttct caacgaatg cttcgcaat cgggggaga 
      gaaaacagt gcccaaccc attcaacca gagaataag 
      tgatcactc agtcagttc attagcgag tgcgatgta 
      acaacatcc gtttgtgct cccaacctt tgaaaaaat 
      ggactcaag tagcagttc cagtatcga gaaatgcaa 
      ctgcgacaa gtcggaacc atcgcaaga aagcgacag 
      tagtaattc ggaatgttc ccccaaaga taagaaaat 
      caaaatcag tctgacttc gctcggtct gaatttgcg 
      cagaggtcg ttcgaaacg taacaagaa aacgaatca 
      aatctcacc gtccacttc ttcgggtgc tggcaccag 
      taaggaaat cacgaagaa gtcgccgaa atggggcat 
      caaattcaa ttgtgctaa gaaagaacc gaaagtggc 
      cttcgtgga agagaacac gaattgttg caagtgcac 
      gtgttacag aaggacgga ggagcacga agcagggcc 
      tgggacagt gtttgcgag tgaggataa tgaaaggac 
      gaatgaggt ggtagagca ttcgaacga ggaggaaaa 
      tagggaaga ggtgcagaa tgagagtga ggaaggcga 
      aggggttaa aggcattta tggcatctc gaggaattc 
      gagctgctt gtgcattgg tccctggga catgcactg 
      gatacgtac tgtacacga agactgtga tgaagccgc 
      tcgattagc gcgtgctcg cgaaatcga gcaaggagt 
      tgatcctcc agcaaattt tcgccccgt acgaagggt 
      gcaagtgct ctgctccga catggactc agaaggcgg 
      ccaagtgcc gcggaggct gcagctggt ctgccctcc 
      agacctaac cgtagacac cctgcgggc ccttttcca 
      aaaccacgt gactctccg gtcctgtcc tttggacac 
      catcgccgg gtgccacac tcaggttga cttcattca 
      ttgtctcgt acctgatct gctggaaat caccaaatg 
      cagttttta ctggggtaa aatggaccg ctacgaggc 
      ggagaggct gctcgacgg taagcccga aggcacttt 
      cctcctccg cgactccgc ccaggaaga gttcctttt 
      cagcgtgtc tttccgcaa gtacggccg gtcgttgca 
      tgcgagaat agaacagtg gaatcataa gttcagttt 
      cgattcgca cgaccccgg cgtctacac gtctgacac 
      agtttgtgg ccttatcga acattacaa agacccgag 
      tagttgcat gttcttcga gccgatgct cacatggcc 
      gttgcacag gaactttac gttttcgct gcaacattt 
      gtgcagagc gacgatcgt taatcggtt gacttacga 
      taatattaa tctgttgca attgcctaa gacgttgaa 
      aagttatct caaagagta ccattatag acagaaagt 
      gagagtgga gaggtttga cgacgacat gcagtggct 
      ggatttgag gaaagtagc ttcgtaatc agtgttatt 
      gttgtacat ctgtttttt tgtttttta tttaattgg 
      agatttgca tgagttgga ggatattgt gcaatagtt 
      tagataact ctggtgacg taaattgtt tttctcatt 
      tttttatct tatttattt aatcgagag acactattt 
      ttatttcaa gatgcaaac tccaattaa attgttgct 
      tgtaactgt cgtaacaat ataacaaaa ttttgtagt 
      aacatatca cagcataag attattaaa gccctccgc 
      tcctagtac ttaattttg taactattt ttctattgc 
      tttaattga aatcaacca caattggat cgtttgcga 
      gggcgaggc tttctcaaa ggctgtgtg ctttatgta 
      ggtactgat tggtagaac gtatttata aacaatctc 
      tcaagttta ctacaaaat tgacaaatg acttacttt 
      tattttata actagctgc ttccaactg ttttgtcag 
      ttttgcttc agttcggtg gaataatac tcattaaat 
      attacatta caaattgct agtcattga acagttaca 
      gccaagcaa tgatggaaa cgtcgatgt cctgttgtg 
      taatggtag tattaagtg gcactaatt tttttcagc 
      tgatgtttg gctgttacg ttaatttaa aatgttagg 
      tgtaagaat ttttgcagt gtgccaaat tgttttgat 
      tttgcagtt gtgaaaacg gtttttaaa ttttgtaac 
      ataaaatat atattttga ctaaaataa tgttgtaat 
      atcagtgta gcaattagc agttaaaga atgtatttt 
      aaatatcga atagcaaat ttagttaat agcttctta 
      ggtaattat ttctaaaag tatattttt aataaaagc 
      attactttc aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY