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Gene information
General information
TC003588 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8153140..8157727
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003588
    iBeetle screen iB_03586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003588 RA
      MNDLDLEEI ESRLYSQVY HSVDTESDL VLPSAPVAP 
      LAPENMKNQ ARYFGPWTP GSKFQKSVH AKQNTRPPT 
      FKDNDQSHP GFYSKSCNR RHRPDNISI NQLVVLTPQ 
      SPIVCVTPK KNRNKARWK NYKRKQKKL EKLANSQKE 
      PPKQTPGVV PGNQEPSNC DKVITLSDS ENEEDSCVL 
      IPENWPVLS VCDSDDDNK KDISENKSS NDDVVFVEP 
      VETPVEVIN LDEETESPK NSQQKNNET ASVASDNSS 
      VSDSPTKKA HRERKELLS TPDSASNDF VNSSGVELN 
      HKNFNFSLH GSDFKSGDF VKPANPNET CETESSSST 
      TDLNSANLM KTIVFNEIE FPKDDVFSE SNLESFGNF 
      ITPMKSRAN TPVAATSST PKVEEQIKQ STNSSDSSS 
      ESDFEDSRV EKVKKIMNL PELSPMQTK EKSKKISSK 
      YDLVDAEAD ESLIKQKQK KKQKNKKKK SEKEETVDC 
      TEAVQKKRK SSQMEDAGD SVLKKKSKN KKKSRTASP 
      ELEANTKSE QKCEGVDVL LPSLKKVTE TDETIKKEH 
      TVKEEPEVK EEPLKIKSP KVVESEESA MDGFLVVDE 
      TFATTTDIK TISSDSDSI LSSLAEDQD IQLINCEAK 
      FEPDCTAST SKSGVLNLA NCSVQDLQF VMSNDPNLW 
      TILDRDRFP IKSPVGKRC NKCKELGHI ALKCPNKLE 
      PKCKLCGEG GHFEPRCPN KMCTQCGKR SYYTTAYCS 
      LCFKLRDYQ CQICSMTGH APETCPDLW RRYHLTTTE 
      GPLKTYSGP ALKPNLWCS GCAQPGHLE HMCDFYKSM 
      YPPTDPFIK NYDQVYDDE NENEPQEEA PQPDPQPNF 
      APNPPQNYV MQGVNQGLN YNCNYDQFA NAFIQNGMF 
      VANQQNFYT NQFAPQDGY MQGYPQYSN AYTSFCDNL 
      PKYISLATP QHTVIATPQ HTVIATPQH TVNLEAPRP 
      EIPSLEFAT PAERKVHCN SMFFSSSGR LLRQFVSRE 
      LAKLSKISF NVKRYVTEL KRRQVPRGA RRRAEYYEL 
      ANMVLFGVL CLGDGKFHL NALKQLKMD KSKLSCFRR 
      QKLHRAYCF IFGCERHDS VDYNELISR I
    CDS sequence
    • >TC003588 RA
      atgaatgac ttggacctt gaagaaatc gaatcacgt 
      ctttatagt caagtttac cactcggtt gataccgaa 
      agcgattta gtccttccg tctgcacca gtcgcgcct 
      ttggccccc gaaaacatg aaaaatcag gcccgttac 
      ttcggccct tggacccct ggcagcaaa ttccaaaaa 
      agtgtgcat gcaaaacaa aacacaaga ccaccaact 
      ttcaaggat aatgaccag tcccatcca gggttttac 
      tcaaaaagt tgcaacagg cgccacagg cctgataat 
      atatcaatc aaccaacta gttgttttg actcctcaa 
      agtccgata gtttgtgta actcctaag aaaaatcgt 
      aataaggcg cgctggaaa aactacaaa aggaagcag 
      aagaaattg gagaaactg gccaattct cagaaggaa 
      cctccgaaa caaacacca ggtgtcgta ccgggaaac 
      caagaacca agtaattgt gacaaggtc attacattg 
      tccgatagt gagaacgag gaggatagc tgtgtgtta 
      attcctgaa aattggccg gttttgtct gtttgtgat 
      tcagacgat gataataag aaagatatt agtgaaaac 
      aagtcgtca aatgacgat gttgtattt gttgagcca 
      gtggagaca cctgttgag gtaataaac cttgacgaa 
      gaaaccgag tctccgaaa aactcacaa caaaaaaat 
      aacgaaact gcgtcagtt gcatcggat aactcgagc 
      gtttccgat tcaccaaca aaaaaggcc caccgcgag 
      cgtaaagaa ttgttaagt acgcccgat tcagcctcg 
      aatgatttc gtcaacagt tctggagtt gaattaaat 
      cacaaaaat ttcaacttc agtttgcac ggatcagat 
      ttcaagagt ggcgacttt gttaaacct gcaaaccct 
      aacgagacg tgcgaaact gagagtagt tcaagcacc 
      actgacctc aattctgca aacttaatg aaaactatc 
      gtttttaac gaaattgaa tttccaaaa gacgacgtt 
      ttctccgag agtaattta gagagtttt ggtaatttt 
      atcactcca atgaagagc cgggcgaat actccggtt 
      gctgcaaca tcgtccact ccgaaggtg gaggagcag 
      attaaacaa tcaactaac tcgtcggat agtagctct 
      gaaagtgat ttcgaggac tcaagagtc gagaaagtt 
      aagaaaatt atgaacttg cccgaacta tcgcccatg 
      cagacgaag gagaaatca aagaaaatt agcagtaaa 
      tacgatttg gtcgatgca gaagcggac gagtcttta 
      ataaaacag aagcagaag aagaaacag aaaaataag 
      aaaaagaag agtgagaaa gaggagact gttgattgc 
      acggaggcg gttcagaag aagagaaaa tcgagccaa 
      atggaagat gcaggtgat agtgtcttg aagaagaaa 
      tctaagaat aagaaaaag tcgcggact gcgtctcct 
      gagcttgaa gcaaatacc aaaagtgag caaaagtgt 
      gaaggtgtt gacgttttg ctccccagt ttaaaaaaa 
      gtaacagaa actgacgaa acaattaaa aaggaacat 
      acagttaag gaagaacca gaagttaag gaagaaccg 
      ttgaagata aagtcgccg aaagtcgtt gaaagtgag 
      gagtctgct atggacggg tttcttgtc gttgatgaa 
      acgtttgcc accaccacc gacatcaaa actatctca 
      tcagattcc gattcgatt ttatcttca cttgctgaa 
      gatcaagac attcaatta ataaattgt gaggcgaaa 
      tttgaacct gactgtact gcgtctact tccaaatcc 
      ggtgtacta aatctggca aattgtagt gtccaagac 
      cttcaattt gtgatgtcg aatgatcct aatctttgg 
      acgatatta gacagggat aggtttcct atcaagtct 
      cctgttggt aaaaggtgc aataaatgc aaagagctg 
      ggtcacata gctctcaaa tgtccaaac aagctggaa 
      cctaagtgc aagctgtgt ggagaaggc gggcatttc 
      gaacctcga tgtcctaac aagatgtgt actcagtgt 
      ggcaaacgt agttactac accacagcc tattgcagc 
      ttgtgtttc aagctacga gattaccaa tgccaaatt 
      tgttccatg actggtcac gcacctgag acttgccca 
      gatctatgg cggcgctac catttaacc accactgag 
      ggtccgttg aaaacgtac tctggccct gccctgaaa 
      ccaaacttg tggtgttct gggtgtgcc cagccaggc 
      cacttggaa cacatgtgt gacttttac aagtcgatg 
      tatccacca acagacccg tttattaaa aactatgat 
      caggtgtat gatgatgag aatgagaat gagccacag 
      gaagaggcg ccgcagcca gatcctcaa ccgaatttc 
      gcaccgaac ccaccccag aattatgta atgcaagga 
      gtcaaccag ggacttaac tataactgc aattacgac 
      caatttgcc aatgcgttt atccaaaat gggatgttt 
      gtggccaac caacaaaac ttctacact aatcagttc 
      gcgccgcaa gacggttac atgcaaggg tatccgcaa 
      tattccaac gcctacacc agtttttgt gacaactta 
      ccgaaatat ataagttta gctactcca cagcacact 
      gtgatagct actccacag cacactgtg atagctact 
      ccacagcac actgtcaat ttggaggcg cctaggcct 
      gaaatccct agcctagaa tttgcgacc cctgctgaa 
      cgaaaagta cactgcaac agcatgttt ttttcatcg 
      tcggggaga ttgttgcga caatttgtc agtagagag 
      ttggcgaaa ttgtccaaa atatcgttc aatgtaaag 
      agatatgtg acagaattg aagaggagg caggttcct 
      cgcggtgca cgacgacgg gcagagtac tacgagttg 
      gccaatatg gtgctcttt ggggttctc tgtttaggg 
      gatgggaaa tttcatctg aacgctctg aaacagctc 
      aaaatggac aagagcaag ttgagttgt ttcagacga 
      caaaaattg catagggcg tactgtttt attttcgga 
      tgtgaaagg cacgacagt gtagactat aatgaactc 
      ataagcagg atttaa
    mRNA sequence
    • >TC003588 RA
      gttggtgac gaaaaccac gtgtgtttc gctttcgct 
      gtcaaatgt tactaattt ttggttcaa gttacaatt 
      tttttctcg tactgttac ttttaaaat gtagattta 
      caactaacc atgaatgac ttggacctt gaagaaatc 
      gaatcacgt ctttatagt caagtttac cactcggtt 
      gataccgaa agcgattta gtccttccg tctgcacca 
      gtcgcgcct ttggccccc gaaaacatg aaaaatcag 
      gcccgttac ttcggccct tggacccct ggcagcaaa 
      ttccaaaaa agtgtgcat gcaaaacaa aacacaaga 
      ccaccaact ttcaaggat aatgaccag tcccatcca 
      gggttttac tcaaaaagt tgcaacagg cgccacagg 
      cctgataat atatcaatc aaccaacta gttgttttg 
      actcctcaa agtccgata gtttgtgta actcctaag 
      aaaaatcgt aataaggcg cgctggaaa aactacaaa 
      aggaagcag aagaaattg gagaaactg gccaattct 
      cagaaggaa cctccgaaa caaacacca ggtgtcgta 
      ccgggaaac caagaacca agtaattgt gacaaggtc 
      attacattg tccgatagt gagaacgag gaggatagc 
      tgtgtgtta attcctgaa aattggccg gttttgtct 
      gtttgtgat tcagacgat gataataag aaagatatt 
      agtgaaaac aagtcgtca aatgacgat gttgtattt 
      gttgagcca gtggagaca cctgttgag gtaataaac 
      cttgacgaa gaaaccgag tctccgaaa aactcacaa 
      caaaaaaat aacgaaact gcgtcagtt gcatcggat 
      aactcgagc gtttccgat tcaccaaca aaaaaggcc 
      caccgcgag cgtaaagaa ttgttaagt acgcccgat 
      tcagcctcg aatgatttc gtcaacagt tctggagtt 
      gaattaaat cacaaaaat ttcaacttc agtttgcac 
      ggatcagat ttcaagagt ggcgacttt gttaaacct 
      gcaaaccct aacgagacg tgcgaaact gagagtagt 
      tcaagcacc actgacctc aattctgca aacttaatg 
      aaaactatc gtttttaac gaaattgaa tttccaaaa 
      gacgacgtt ttctccgag agtaattta gagagtttt 
      ggtaatttt atcactcca atgaagagc cgggcgaat 
      actccggtt gctgcaaca tcgtccact ccgaaggtg 
      gaggagcag attaaacaa tcaactaac tcgtcggat 
      agtagctct gaaagtgat ttcgaggac tcaagagtc 
      gagaaagtt aagaaaatt atgaacttg cccgaacta 
      tcgcccatg cagacgaag gagaaatca aagaaaatt 
      agcagtaaa tacgatttg gtcgatgca gaagcggac 
      gagtcttta ataaaacag aagcagaag aagaaacag 
      aaaaataag aaaaagaag agtgagaaa gaggagact 
      gttgattgc acggaggcg gttcagaag aagagaaaa 
      tcgagccaa atggaagat gcaggtgat agtgtcttg 
      aagaagaaa tctaagaat aagaaaaag tcgcggact 
      gcgtctcct gagcttgaa gcaaatacc aaaagtgag 
      caaaagtgt gaaggtgtt gacgttttg ctccccagt 
      ttaaaaaaa gtaacagaa actgacgaa acaattaaa 
      aaggaacat acagttaag gaagaacca gaagttaag 
      gaagaaccg ttgaagata aagtcgccg aaagtcgtt 
      gaaagtgag gagtctgct atggacggg tttcttgtc 
      gttgatgaa acgtttgcc accaccacc gacatcaaa 
      actatctca tcagattcc gattcgatt ttatcttca 
      cttgctgaa gatcaagac attcaatta ataaattgt 
      gaggcgaaa tttgaacct gactgtact gcgtctact 
      tccaaatcc ggtgtacta aatctggca aattgtagt 
      gtccaagac cttcaattt gtgatgtcg aatgatcct 
      aatctttgg acgatatta gacagggat aggtttcct 
      atcaagtct cctgttggt aaaaggtgc aataaatgc 
      aaagagctg ggtcacata gctctcaaa tgtccaaac 
      aagctggaa cctaagtgc aagctgtgt ggagaaggc 
      gggcatttc gaacctcga tgtcctaac aagatgtgt 
      actcagtgt ggcaaacgt agttactac accacagcc 
      tattgcagc ttgtgtttc aagctacga gattaccaa 
      tgccaaatt tgttccatg actggtcac gcacctgag 
      acttgccca gatctatgg cggcgctac catttaacc 
      accactgag ggtccgttg aaaacgtac tctggccct 
      gccctgaaa ccaaacttg tggtgttct gggtgtgcc 
      cagccaggc cacttggaa cacatgtgt gacttttac 
      aagtcgatg tatccacca acagacccg tttattaaa 
      aactatgat caggtgtat gatgatgag aatgagaat 
      gagccacag gaagaggcg ccgcagcca gatcctcaa 
      ccgaatttc gcaccgaac ccaccccag aattatgta 
      atgcaagga gtcaaccag ggacttaac tataactgc 
      aattacgac caatttgcc aatgcgttt atccaaaat 
      gggatgttt gtggccaac caacaaaac ttctacact 
      aatcagttc gcgccgcaa gacggttac atgcaaggg 
      tatccgcaa tattccaac gcctacacc agtttttgt 
      gacaactta ccgaaatat ataagttta gctactcca 
      cagcacact gtgatagct actccacag cacactgtg 
      atagctact ccacagcac actgtcaat ttggaggcg 
      cctaggcct gaaatccct agcctagaa tttgcgacc 
      cctgctgaa cgaaaagta cactgcaac agcatgttt 
      ttttcatcg tcggggaga ttgttgcga caatttgtc 
      agtagagag ttggcgaaa ttgtccaaa atatcgttc 
      aatgtaaag agatatgtg acagaattg aagaggagg 
      caggttcct cgcggtgca cgacgacgg gcagagtac 
      tacgagttg gccaatatg gtgctcttt ggggttctc 
      tgtttaggg gatgggaaa tttcatctg aacgctctg 
      aaacagctc aaaatggac aagagcaag ttgagttgt 
      ttcagacga caaaaattg catagggcg tactgtttt 
      attttcgga tgtgaaagg cacgacagt gtagactat 
      aatgaactc ataagcagg atttaatga attttaatt 
      tgcgatttt tgaataatt ttttgttga aataaatac 
      gaattttcc gatcttggt gttttattt ccaaggagt 
      gctctttaa gggggttgc tagtgtgcg aaaggtttg 
      gcttccgat cgcgggatg gcgctgttg acagacagc 
      caactatga ggtaatcag ctgatcgtc gatgacatt 
      attttccaa agccgacta tgtattatg acaggaggg 
      agagagact attggaaaa actatgtct ctgacagag 
      acaaacagt agtgtagaa aatggcagc agtaggtac 
      aacaaattt tgattgttt agaaattaa gacacgtag 
      tatagaaca aacccttct tcctcttat taaacaggt 
      aattttgtg ttagctgct ttctgtcat gtgaacttt 
      agttagcac tttatttag ttaacttag gtttagaaa 
      atgtgtctc gcgacatgg gtgtgcact ttgacagct 
      ctcaattaa cgtgctagc tcgcataat tgaatattg 
      ttatgtatg aaaacattg ttgctgttt atttatagc 
      attgtgtat agatattag atgaacgct atgcataaa 
      atagctcga gttttttca aataaagca gtttgaggt 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY