| General information | |||
| TC034592 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 8055204..8070838 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034592 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03585 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03584 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034592 RA
MRSPEHRNT YNRTGHTQD EVSSRHKAP GAFTILPPD DAAPQDPPM APQPYRLTP SSRRHKNMS FAILAEPSS LPTSRSPSP TGRVSPFRG RGFNPIGSR HASPAPSPP PQGAFTLQE SPSHSKIPR LSRRGSAAA FGEKITDYQ SSPNRRKLN AQLSQSLTN IAPRRVTAR PPPSPRRPP GYSKNTFQR LSPIVGSSP EPSQSQSSP KLNTSSTVS KSRSFVNRP PRNASKPTS KATSREPSP SKNPASPTR IPVKNYRNV QAKVNSFSR TKPKVPPKP QVLSETDSD SVANRRKVE RKESVSRLR TNSRINLNL NNNNNTKNN NKNNKNNVG KDTNGNQKS NNNNNNKNA VTNKGAESS KEKNETKAT NDKGNNKKE DSTKKNEAK EETAKKSEK ESPVKLSDL LQPSTTAVV SSTTTTVTQ PLKIEAKIE EPAKTDNKM APMVDGRIL SATSVSHAM NKMNDTVLD SQTLMKESG LTKLSPAAS TIIAMSTKP SRHQXDSQT LMKESGLTK LSPAASTII AMSNQNPLD TKNATTALP KVEEATTKT VDSINSNHV TSTGIVPAR VLEQHVQNN MSKLISPDH KIGNSVQKT ANDRIIEAR TVVAHDVKP IKITVREKP MDVEVQSGN IRFDTSNGL GERPGLPPP QQPNDSQEP QEPPKGNAC TRFLSTCKK KMSCKRCKK QKDETKDEL KKEREETGK TGCFNCRKK KPDVTINIE NDEPKPKFW DRMKCCKRN KVGDTGCFP TGKRKESWV GERRDSILS DPPTVQKSR CSGFFRRMF CLDKCRKKT PPKIEETQE SRRASMMSK KKSLTPSVP VEDTKPKLD LSLVEHTSH MKAAIPVLP VCLAWFCLV MNCIAPGTG TVFSGMFCL CIGKPRFSQ KDGPRPRIG AFIIDLIIG FGQFFTVLF CLVGWGWSI WWGVIMVKL AKKNKKIKA LEANAQEGS RQVPAANQN HRDPERGS
- >TC034592 RB
MRSPEHRNT YNRTGHTQD EVSSRHKAP GAFTILPPD DAAPQDPPM APQPYRLTP SSRRHKNMS FAILAEPSS LPTSRSPSP TGRVSPFRG RGFNPIGSR HASPAPSPP PQGAFTLQE SPSHSKIPR LSRRGSAAA FGEKITDYQ SSPNRRKLN AQLSQSLTN IAPRRVTAR PPPSPRRPP GYSKNTFQR LSPIVGSSP EPSQSQSSP KLNTSSTVS KSRSFVNRP PRNASKPTS KATSREPSP SKNPASPTR IPVKNYRNV QAKVNSFSR TKPKVPPKP QVLSETDSD SVANRRKVE RKESVSKDT NGNQKSNNN NNNKNAVTN KGAESSKEK NETKATNDK GNNKKEDST KKNEAKEET AKKSEKESP VKLSDLLQP STTAVVSST TTTVTQPLK IEAKIEEPA KTDNKMAPM VDGRILSAT SVSHAMNKM NDTVLDSQT LMKESGLTK LSPAASTII AMSTKPSRH QXDSQTLMK ESGLTKLSP AASTIIAMS NQNPLDTKN ATTALPKVE EATTKTVDS INSNHVTST GIVPARVLE QHVQNNMSK LISPDHKIG NSVQKTAND RIIEARTVV AHDVKPIKI TVREKPMDV EVQSGNIRF DTSNGLGER PGLPPPQQP NDSQEPQEP PKGNACTRF LSTCKKKMS CKRCKKQKD ETKDELKKE REETGKTGC FNCRKKKPD VTINIENDE PKPKFWDRM KCCKRNKVG DTGCFPTGK RKESWVGER RDSILSDPP TVQKSRCSG FFRRMFCLD KCRKKTPPK IEETQESRR ASMMSKKKS LTPSVPVED TKPKLDLSL VEHTSHMKA AIPVLPVCL AWFCLVMNC IAPGTGTVF SGMFCLCIG KPRFSQKDG PRPRIGAFI IDLIIGFGQ FFTVLFCLV GWGWSIWWG VIMVKLAKK NKKIKALEA NAQEGSRQV PAANQNHRD PERGS
- >TC034592 RC
MRSPEHRNT YNRTGHTQD EVSSRHKAP GAFTILPPD DAAPQDPPM APQPYRLTP SSRRHKNMS FAILAEPSS LPTSRSPSP TGRVSPFRG RGFNPIGSR HASPAPSPP PQGAFTLQE SPSHSKIPR LSRRGSAAA FGEKITDYQ SSPNRRKLN AQLSQSLTN IAPRRVTAR PPPSPRRPP GYSKNTFQR LSPIVGSSP EPSQSQSSP KLNTSSTVS KSRSFVNRP PRNASKPTS KATSREPSP SKNPASPTR IPVKNYRNV QAKVNSFSR TKPKVPPKP QVLSETDSD SVANRRKVE RKESVSRLR TNSRINLNL NNNNNTKNN NKNNKNNVG KDTNGNQKS NNNNNNKNA VTNKGAESS KEKNETKAT NDKGNNKKE DSTKKNEAK EETAKKSEK ESPVKLSDL LQPSTTAVV SSTTTTVTQ PLKIEAKIE EPAKTDNKM APMVDGRIL SATSVSHAM NKMNDTVLD SQTLMKESG LTKLSPAAS TIIAMSTKP SRHQXDSQT LMKESGLTK LSPAASTII AMSNQNPLD TKNATTALP KVEEATTKT VDSINSNHV TSTGIVPAR VLEQHVQNN MSKLISPDH KIGNSVQKT ANDRIIEAR TVVAHDVKP IKITVREKP MDVEVQSGN IRFDTSNGL GERPGGISG ELVRVKKLI FNRMTRERE SSREQIAKM TYIYVMPLH EEYNGYGVL ENGGLPPPQ QPNDSQEPQ EPPKGNACT RFLSTCKKK MSCKRCKKQ KDETKDELK KEREETGKT GCFNCRKKK PDVTINIEN DEPKPKFWD RMKCCKRNK VGDTGCFPT GKRKESWVG ERRDSILSD PPTVQKSRC SGFFRRMFC LDKCRKKTP PKIEETQES RRASMMSKK KSLTPSVPV EDTKPKLDL SLVEHTSHM KAAIPVLPV CLAWFCLVM NCIAPGTGT VFSGMFCLC IGKPRFSQK DGPRPRIGA FIIDLIIGF GQFFTVLFC LVGWGWSIW WGVIMVKLA KKNKKIKAL EANAQEGSR QVPAANQNH RDPERGS
- >TC034592 RA
atgcgttcg cccgaacac agaaacacg tacaataga acgggacac actcaagat gaagtatct tcacgtcat aaggcacca ggagctttt accatactc ccacccgat gatgcggcc cctcaagac cctcctatg gctcctcag ccatacagg cttactcca tcctcccga agacacaag aacatgtct ttcgcgatc ctggcggag cccagcagc ttgcccacc tcgcggagc cccagcccc accggccgc gttagcccc ttccggggc cggggcttc aaccccatc ggctcgcgg cacgcgtcc ccggccccg tcaccgccg ccccagggg gctttcacc ctccaggag tcccccagc cactccaaa atcccgcga ctgtcccgc cgcggaagc gccgccgct ttcggggag aaaatcacc gattaccaa tcgtccccc aacaggaga aaattgaac gcacagctt agtcaaagc ctaactaac attgcgccc agacgggtc accgcccgg ccgccgccg agcccccga aggccccca gggtactcc aaaaacacc ttccagagg ctttccccc atcgtaggt agttcgcct gagccaagt caatcccag tcttcccct aaactcaac actagtagt acagtgagc aagagtcgc tcgtttgta aatagaccg ccgaggaat gcctccaag cccacctcc aaggccacc agtagggag ccgagtcct agcaagaac ccagcttcg cccacgagg atcccggtc aagaattac agaaatgtg caagcgaaa gttaatagt tttagtagg actaaacca aaagtgcct cctaaaccc caagtgctg agcgagacc gattcggat agtgtggct aatagaaga aaagtggaa cgaaaagaa agtgttagt aggttaagg acaaactct agaatcaat ctaaatctt aataacaat aataatacg aaaaacaat aataaaaac aataaaaat aacgtaggt aaagacact aacgggaac cagaaaagt aataataac aacaataat aaaaatgct gtaacaaat aaaggtgcc gaatcgagt aaagagaaa aatgaaacg aaggcaaca aacgacaaa gggaacaat aaaaaagaa gactccacc aagaagaat gaggcaaaa gaagaaacg gccaaaaaa tcagaaaaa gaaagtccg gtgaaactg tccgacttg ctccaaccc tcgacgaca gctgtggtt tcaagcacc acaaccaca gtaacccaa cccttgaaa atcgaagcc aaaatcgaa gaacctgca aaaaccgac aataaaatg gcacccatg gtcgacggc cgcatcttg agtgcaacc agcgtctcc catgcgatg aataaaatg aacgatacg gtccttgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtca accaaaccc tctagacac caaantgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtcc aaccaaaac cctctagac accaaaaat gcgaccacg gctctgccg aaagtcgaa gaagccaca actaagacc gtggacagt ataaacagt aaccacgtt acttcgact ggaatcgtg ccagcgagg gttttggag caacacgtc cagaacaac atgagcaaa ttgataagt ccggaccac aaaatcggg aattccgtc cagaaaacc gccaacgat aggataatc gaagcacgg actgtcgtc gcccatgat gtcaagccc atcaagatt actgtgagg gagaaaccc atggatgtg gaggtacag agcgggaat atccgcttc gacacgtcc aacggcttg ggggagagg ccgggttta ccccctcct cagcagccc aacgactcg caagaaccc caagaacca cccaaaggc aatgcatgc acgcgattt ctttcaaca tgcaaaaag aaaatgtca tgtaagcga tgcaagaaa caaaaagac gaaactaaa gacgagctc aagaaagaa cgcgaagaa actggaaaa acgggatgc ttcaactgc aggaagaag aaacctgac gtcaccatc aacattgag aatgacgaa cccaagccg aaattttgg gaccggatg aagtgctgc aagagaaac aaggttggc gacactggg tgcttcccg acgggaaaa cggaaggag agctgggtg ggggagaga agagacagc attttgagc gatccgcca actgttcaa aagtcgcga tgttccggc ttcttccgc aggatgttc tgtttggac aaatgccgc aaaaaaacg ccgcccaaa atcgaggaa acgcaagaa agtcgaagg gcttccatg atgagcaaa aagaagagt ctaacgcct tcagtcccc gttgaggac accaaacca aaacttgat ctcagtttg gtggaacac acgtcccac atgaaagcg gcaattccc gttcttcca gtttgttta gcttggttc tgtcttgtc atgaactgc atcgcgccc gggacaggg accgttttc agcggaatg ttctgctta tgcatcggc aagccgcgg ttttcgcag aaagacggc ccccggccc cgcatcggt gccttcatc atcgacctc atcatcggc ttcggccag tttttcacc gttttgttt tgtctggtc gggtggggt tggtccatt tggtggggc gtgattatg gtgaaactt gccaaaaaa aataaaaaa attaaagcc ttagaagca aacgcgcaa gaagggtcc agacaagtg ccagccgcc aatcagaac caccgagat ccggaaagg gggtcttga
- >TC034592 RB
atgcgttcg cccgaacac agaaacacg tacaataga acgggacac actcaagat gaagtatct tcacgtcat aaggcacca ggagctttt accatactc ccacccgat gatgcggcc cctcaagac cctcctatg gctcctcag ccatacagg cttactcca tcctcccga agacacaag aacatgtct ttcgcgatc ctggcggag cccagcagc ttgcccacc tcgcggagc cccagcccc accggccgc gttagcccc ttccggggc cggggcttc aaccccatc ggctcgcgg cacgcgtcc ccggccccg tcaccgccg ccccagggg gctttcacc ctccaggag tcccccagc cactccaaa atcccgcga ctgtcccgc cgcggaagc gccgccgct ttcggggag aaaatcacc gattaccaa tcgtccccc aacaggaga aaattgaac gcacagctt agtcaaagc ctaactaac attgcgccc agacgggtc accgcccgg ccgccgccg agcccccga aggccccca gggtactcc aaaaacacc ttccagagg ctttccccc atcgtaggt agttcgcct gagccaagt caatcccag tcttcccct aaactcaac actagtagt acagtgagc aagagtcgc tcgtttgta aatagaccg ccgaggaat gcctccaag cccacctcc aaggccacc agtagggag ccgagtcct agcaagaac ccagcttcg cccacgagg atcccggtc aagaattac agaaatgtg caagcgaaa gttaatagt tttagtagg actaaacca aaagtgcct cctaaaccc caagtgctg agcgagacc gattcggat agtgtggct aatagaaga aaagtggaa cgaaaagaa agtgttagt aaagacact aacgggaac cagaaaagt aataataac aacaataat aaaaatgct gtaacaaat aaaggtgcc gaatcgagt aaagagaaa aatgaaacg aaggcaaca aacgacaaa gggaacaat aaaaaagaa gactccacc aagaagaat gaggcaaaa gaagaaacg gccaaaaaa tcagaaaaa gaaagtccg gtgaaactg tccgacttg ctccaaccc tcgacgaca gctgtggtt tcaagcacc acaaccaca gtaacccaa cccttgaaa atcgaagcc aaaatcgaa gaacctgca aaaaccgac aataaaatg gcacccatg gtcgacggc cgcatcttg agtgcaacc agcgtctcc catgcgatg aataaaatg aacgatacg gtccttgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtca accaaaccc tctagacac caaantgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtcc aaccaaaac cctctagac accaaaaat gcgaccacg gctctgccg aaagtcgaa gaagccaca actaagacc gtggacagt ataaacagt aaccacgtt acttcgact ggaatcgtg ccagcgagg gttttggag caacacgtc cagaacaac atgagcaaa ttgataagt ccggaccac aaaatcggg aattccgtc cagaaaacc gccaacgat aggataatc gaagcacgg actgtcgtc gcccatgat gtcaagccc atcaagatt actgtgagg gagaaaccc atggatgtg gaggtacag agcgggaat atccgcttc gacacgtcc aacggcttg ggggagagg ccgggttta ccccctcct cagcagccc aacgactcg caagaaccc caagaacca cccaaaggc aatgcatgc acgcgattt ctttcaaca tgcaaaaag aaaatgtca tgtaagcga tgcaagaaa caaaaagac gaaactaaa gacgagctc aagaaagaa cgcgaagaa actggaaaa acgggatgc ttcaactgc aggaagaag aaacctgac gtcaccatc aacattgag aatgacgaa cccaagccg aaattttgg gaccggatg aagtgctgc aagagaaac aaggttggc gacactggg tgcttcccg acgggaaaa cggaaggag agctgggtg ggggagaga agagacagc attttgagc gatccgcca actgttcaa aagtcgcga tgttccggc ttcttccgc aggatgttc tgtttggac aaatgccgc aaaaaaacg ccgcccaaa atcgaggaa acgcaagaa agtcgaagg gcttccatg atgagcaaa aagaagagt ctaacgcct tcagtcccc gttgaggac accaaacca aaacttgat ctcagtttg gtggaacac acgtcccac atgaaagcg gcaattccc gttcttcca gtttgttta gcttggttc tgtcttgtc atgaactgc atcgcgccc gggacaggg accgttttc agcggaatg ttctgctta tgcatcggc aagccgcgg ttttcgcag aaagacggc ccccggccc cgcatcggt gccttcatc atcgacctc atcatcggc ttcggccag tttttcacc gttttgttt tgtctggtc gggtggggt tggtccatt tggtggggc gtgattatg gtgaaactt gccaaaaaa aataaaaaa attaaagcc ttagaagca aacgcgcaa gaagggtcc agacaagtg ccagccgcc aatcagaac caccgagat ccggaaagg gggtcttga
- >TC034592 RC
atgcgttcg cccgaacac agaaacacg tacaataga acgggacac actcaagat gaagtatct tcacgtcat aaggcacca ggagctttt accatactc ccacccgat gatgcggcc cctcaagac cctcctatg gctcctcag ccatacagg cttactcca tcctcccga agacacaag aacatgtct ttcgcgatc ctggcggag cccagcagc ttgcccacc tcgcggagc cccagcccc accggccgc gttagcccc ttccggggc cggggcttc aaccccatc ggctcgcgg cacgcgtcc ccggccccg tcaccgccg ccccagggg gctttcacc ctccaggag tcccccagc cactccaaa atcccgcga ctgtcccgc cgcggaagc gccgccgct ttcggggag aaaatcacc gattaccaa tcgtccccc aacaggaga aaattgaac gcacagctt agtcaaagc ctaactaac attgcgccc agacgggtc accgcccgg ccgccgccg agcccccga aggccccca gggtactcc aaaaacacc ttccagagg ctttccccc atcgtaggt agttcgcct gagccaagt caatcccag tcttcccct aaactcaac actagtagt acagtgagc aagagtcgc tcgtttgta aatagaccg ccgaggaat gcctccaag cccacctcc aaggccacc agtagggag ccgagtcct agcaagaac ccagcttcg cccacgagg atcccggtc aagaattac agaaatgtg caagcgaaa gttaatagt tttagtagg actaaacca aaagtgcct cctaaaccc caagtgctg agcgagacc gattcggat agtgtggct aatagaaga aaagtggaa cgaaaagaa agtgttagt aggttaagg acaaactct agaatcaat ctaaatctt aataacaat aataatacg aaaaacaat aataaaaac aataaaaat aacgtaggt aaagacact aacgggaac cagaaaagt aataataac aacaataat aaaaatgct gtaacaaat aaaggtgcc gaatcgagt aaagagaaa aatgaaacg aaggcaaca aacgacaaa gggaacaat aaaaaagaa gactccacc aagaagaat gaggcaaaa gaagaaacg gccaaaaaa tcagaaaaa gaaagtccg gtgaaactg tccgacttg ctccaaccc tcgacgaca gctgtggtt tcaagcacc acaaccaca gtaacccaa cccttgaaa atcgaagcc aaaatcgaa gaacctgca aaaaccgac aataaaatg gcacccatg gtcgacggc cgcatcttg agtgcaacc agcgtctcc catgcgatg aataaaatg aacgatacg gtccttgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtca accaaaccc tctagacac caaantgac tctcaaacc ctgatgaag gaatccgga cttacgaaa ctatcaccg gctgcaagc accatcatc gcgatgtcc aaccaaaac cctctagac accaaaaat gcgaccacg gctctgccg aaagtcgaa gaagccaca actaagacc gtggacagt ataaacagt aaccacgtt acttcgact ggaatcgtg ccagcgagg gttttggag caacacgtc cagaacaac atgagcaaa ttgataagt ccggaccac aaaatcggg aattccgtc cagaaaacc gccaacgat aggataatc gaagcacgg actgtcgtc gcccatgat gtcaagccc atcaagatt actgtgagg gagaaaccc atggatgtg gaggtacag agcgggaat atccgcttc gacacgtcc aacggcttg ggggagagg ccgggtgga atatcgggt gaacttgtg cgtgtgaaa aagctgatt ttcaataga atgacccga gagagggaa agttcaagg gagcagatt gcgaaaatg acgtacatc tacgttatg cccttacat gaggagtat aatggttac ggagttctg gaaaatgga ggtttaccc cctcctcag cagcccaac gactcgcaa gaaccccaa gaaccaccc aaaggcaat gcatgcacg cgatttctt tcaacatgc aaaaagaaa atgtcatgt aagcgatgc aagaaacaa aaagacgaa actaaagac gagctcaag aaagaacgc gaagaaact ggaaaaacg ggatgcttc aactgcagg aagaagaaa cctgacgtc accatcaac attgagaat gacgaaccc aagccgaaa ttttgggac cggatgaag tgctgcaag agaaacaag gttggcgac actgggtgc ttcccgacg ggaaaacgg aaggagagc tgggtgggg gagagaaga gacagcatt ttgagcgat ccgccaact gttcaaaag tcgcgatgt tccggcttc ttccgcagg atgttctgt ttggacaaa tgccgcaaa aaaacgccg cccaaaatc gaggaaacg caagaaagt cgaagggct tccatgatg agcaaaaag aagagtcta acgccttca gtccccgtt gaggacacc aaaccaaaa cttgatctc agtttggtg gaacacacg tcccacatg aaagcggca attcccgtt cttccagtt tgtttagct tggttctgt cttgtcatg aactgcatc gcgcccggg acagggacc gttttcagc ggaatgttc tgcttatgc atcggcaag ccgcggttt tcgcagaaa gacggcccc cggccccgc atcggtgcc ttcatcatc gacctcatc atcggcttc ggccagttt ttcaccgtt ttgttttgt ctggtcggg tggggttgg tccatttgg tggggcgtg attatggtg aaacttgcc aaaaaaaat aaaaaaatt aaagcctta gaagcaaac gcgcaagaa gggtccaga caagtgcca gccgccaat cagaaccac cgagatccg gaaaggggg tcttga
- >TC034592 RA
ttcctgagc tttcgcagg gctcaaaaa atcattttg ggcactatg aacaaaaat tcgaagcgg gaattgtga ctaagtggt gacggcaga ttaaggctc caacatggc tgggagcac accaactgg gagaatatg aagatcagg gcaaagtcg tagcaattt ttagttttt ttcgttcca aaaatgcgt tcgcccgaa cacagaaac acgtacaat agaacggga cacactcaa gatgaagta tcttcacgt cataaggca ccaggagct tttaccata ctcccaccc gatgatgcg gcccctcaa gaccctcct atggctcct cagccatac aggcttact ccatcctcc cgaagacac aagaacatg tctttcgcg atcctggcg gagcccagc agcttgccc acctcgcgg agccccagc cccaccggc cgcgttagc cccttccgg ggccggggc ttcaacccc atcggctcg cggcacgcg tccccggcc ccgtcaccg ccgccccag ggggctttc accctccag gagtccccc agccactcc aaaatcccg cgactgtcc cgccgcgga agcgccgcc gctttcggg gagaaaatc accgattac caatcgtcc cccaacagg agaaaattg aacgcacag cttagtcaa agcctaact aacattgcg cccagacgg gtcaccgcc cggccgccg ccgagcccc cgaaggccc ccagggtac tccaaaaac accttccag aggctttcc cccatcgta ggtagttcg cctgagcca agtcaatcc cagtcttcc cctaaactc aacactagt agtacagtg agcaagagt cgctcgttt gtaaataga ccgccgagg aatgcctcc aagcccacc tccaaggcc accagtagg gagccgagt cctagcaag aacccagct tcgcccacg aggatcccg gtcaagaat tacagaaat gtgcaagcg aaagttaat agttttagt aggactaaa ccaaaagtg cctcctaaa ccccaagtg ctgagcgag accgattcg gatagtgtg gctaataga agaaaagtg gaacgaaaa gaaagtgtt agtaggtta aggacaaac tctagaatc aatctaaat cttaataac aataataat acgaaaaac aataataaa aacaataaa aataacgta ggtaaagac actaacggg aaccagaaa agtaataat aacaacaat aataaaaat gctgtaaca aataaaggt gccgaatcg agtaaagag aaaaatgaa acgaaggca acaaacgac aaagggaac aataaaaaa gaagactcc accaagaag aatgaggca aaagaagaa acggccaaa aaatcagaa aaagaaagt ccggtgaaa ctgtccgac ttgctccaa ccctcgacg acagctgtg gtttcaagc accacaacc acagtaacc caacccttg aaaatcgaa gccaaaatc gaagaacct gcaaaaacc gacaataaa atggcaccc atggtcgac ggccgcatc ttgagtgca accagcgtc tcccatgcg atgaataaa atgaacgat acggtcctt gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tcaaccaaa ccctctaga caccaaant gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tccaaccaa aaccctcta gacaccaaa aatgcgacc acggctctg ccgaaagtc gaagaagcc acaactaag accgtggac agtataaac agtaaccac gttacttcg actggaatc gtgccagcg agggttttg gagcaacac gtccagaac aacatgagc aaattgata agtccggac cacaaaatc gggaattcc gtccagaaa accgccaac gataggata atcgaagca cggactgtc gtcgcccat gatgtcaag cccatcaag attactgtg agggagaaa cccatggat gtggaggta cagagcggg aatatccgc ttcgacacg tccaacggc ttgggggag aggccgggt ttaccccct cctcagcag cccaacgac tcgcaagaa ccccaagaa ccacccaaa ggcaatgca tgcacgcga tttctttca acatgcaaa aagaaaatg tcatgtaag cgatgcaag aaacaaaaa gacgaaact aaagacgag ctcaagaaa gaacgcgaa gaaactgga aaaacggga tgcttcaac tgcaggaag aagaaacct gacgtcacc atcaacatt gagaatgac gaacccaag ccgaaattt tgggaccgg atgaagtgc tgcaagaga aacaaggtt ggcgacact gggtgcttc ccgacggga aaacggaag gagagctgg gtgggggag agaagagac agcattttg agcgatccg ccaactgtt caaaagtcg cgatgttcc ggcttcttc cgcaggatg ttctgtttg gacaaatgc cgcaaaaaa acgccgccc aaaatcgag gaaacgcaa gaaagtcga agggcttcc atgatgagc aaaaagaag agtctaacg ccttcagtc cccgttgag gacaccaaa ccaaaactt gatctcagt ttggtggaa cacacgtcc cacatgaaa gcggcaatt cccgttctt ccagtttgt ttagcttgg ttctgtctt gtcatgaac tgcatcgcg cccgggaca gggaccgtt ttcagcgga atgttctgc ttatgcatc ggcaagccg cggttttcg cagaaagac ggcccccgg ccccgcatc ggtgccttc atcatcgac ctcatcatc ggcttcggc cagtttttc accgttttg ttttgtctg gtcgggtgg ggttggtcc atttggtgg ggcgtgatt atggtgaaa cttgccaaa aaaaataaa aaaattaaa gccttagaa gcaaacgcg caagaaggg tccagacaa gtgccagcc gccaatcag aaccaccga gatccggaa agggggtct tgaacagcg gctaaagac atatcactt cttctggaa ggttttttc tacaatatt ttgtacagg ctttcgcac aaattacaa aactttcga tttagcccc aaattgtta tcgttaaac ccatttttg ctggtcaaa aatgttttg taagaagtg tagatagga acaaaatta cgcaagtta cactatttt ttgtaaact aacatgtag aaatgtgta ttatacatt ttatagcgc aggtggggg ttgttactt aacatgtat ttggacaaa gtgtacaaa gctttttca aagtattta aacacttta ttatgaata caataacaa tgcaatacc agctaagta caagcacac agttacaga ttacagaag tatttttct ataaagtta ggctaaatt ggcgtgttt aaatacttt atgtacact tttttaata aagatttat tgtaaataa aacgcgttt ttatttcaa agtacttac cagctaggt gtcttgaat tttacctca tttttattg gatcatggg atagggggc cggaagtgg ctgtgagga ttgatctta cacttcaaa aacacatta attggtaat taatttact tatttcagc gatgaaata taacttatt tacattatc gaaaaaacg aattgactt tttagtaat actgaacct agccattct atccttgta gatggtatg ttccaatat gtacataat tttttaaaa ttcaatatg aaaaactgt cttgggaac aatacatgt acaaaaata aaatgccag aaaatattt ggcatacaa ttacgcgta tattataat actttgaat aatttaaat atatacaca cagcgtcgg aaaatacat acatttact taacattac ttgtgtatt atgatgttt cagcttgtc atgtctgta cgcatgttg atgtatttt tgaacgtgt aagcattgt aggtatggt gtgttttgt ctccctctg tgatattaa ataaatttt aaaaaataa tattcgttc gatatcgaa acacaaagg gaaaacttt cgatcatta ggtttcgtt gcccctccc gcgtaaatc ccag
- >TC034592 RB
ttcctgagc tttcgcagg gctcaaaaa atcattttg ggcactatg aacaaaaat tcgaagcgg gaattgtga ctaagtggt gacggcaga ttaaggctc caacatggc tgggagcac accaactgg gagaatatg aagatcagg gcaaagtcg tagcaattt ttagttttt ttcgttcca aaaatgcgt tcgcccgaa cacagaaac acgtacaat agaacggga cacactcaa gatgaagta tcttcacgt cataaggca ccaggagct tttaccata ctcccaccc gatgatgcg gcccctcaa gaccctcct atggctcct cagccatac aggcttact ccatcctcc cgaagacac aagaacatg tctttcgcg atcctggcg gagcccagc agcttgccc acctcgcgg agccccagc cccaccggc cgcgttagc cccttccgg ggccggggc ttcaacccc atcggctcg cggcacgcg tccccggcc ccgtcaccg ccgccccag ggggctttc accctccag gagtccccc agccactcc aaaatcccg cgactgtcc cgccgcgga agcgccgcc gctttcggg gagaaaatc accgattac caatcgtcc cccaacagg agaaaattg aacgcacag cttagtcaa agcctaact aacattgcg cccagacgg gtcaccgcc cggccgccg ccgagcccc cgaaggccc ccagggtac tccaaaaac accttccag aggctttcc cccatcgta ggtagttcg cctgagcca agtcaatcc cagtcttcc cctaaactc aacactagt agtacagtg agcaagagt cgctcgttt gtaaataga ccgccgagg aatgcctcc aagcccacc tccaaggcc accagtagg gagccgagt cctagcaag aacccagct tcgcccacg aggatcccg gtcaagaat tacagaaat gtgcaagcg aaagttaat agttttagt aggactaaa ccaaaagtg cctcctaaa ccccaagtg ctgagcgag accgattcg gatagtgtg gctaataga agaaaagtg gaacgaaaa gaaagtgtt agtaaagac actaacggg aaccagaaa agtaataat aacaacaat aataaaaat gctgtaaca aataaaggt gccgaatcg agtaaagag aaaaatgaa acgaaggca acaaacgac aaagggaac aataaaaaa gaagactcc accaagaag aatgaggca aaagaagaa acggccaaa aaatcagaa aaagaaagt ccggtgaaa ctgtccgac ttgctccaa ccctcgacg acagctgtg gtttcaagc accacaacc acagtaacc caacccttg aaaatcgaa gccaaaatc gaagaacct gcaaaaacc gacaataaa atggcaccc atggtcgac ggccgcatc ttgagtgca accagcgtc tcccatgcg atgaataaa atgaacgat acggtcctt gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tcaaccaaa ccctctaga caccaaant gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tccaaccaa aaccctcta gacaccaaa aatgcgacc acggctctg ccgaaagtc gaagaagcc acaactaag accgtggac agtataaac agtaaccac gttacttcg actggaatc gtgccagcg agggttttg gagcaacac gtccagaac aacatgagc aaattgata agtccggac cacaaaatc gggaattcc gtccagaaa accgccaac gataggata atcgaagca cggactgtc gtcgcccat gatgtcaag cccatcaag attactgtg agggagaaa cccatggat gtggaggta cagagcggg aatatccgc ttcgacacg tccaacggc ttgggggag aggccgggt ttaccccct cctcagcag cccaacgac tcgcaagaa ccccaagaa ccacccaaa ggcaatgca tgcacgcga tttctttca acatgcaaa aagaaaatg tcatgtaag cgatgcaag aaacaaaaa gacgaaact aaagacgag ctcaagaaa gaacgcgaa gaaactgga aaaacggga tgcttcaac tgcaggaag aagaaacct gacgtcacc atcaacatt gagaatgac gaacccaag ccgaaattt tgggaccgg atgaagtgc tgcaagaga aacaaggtt ggcgacact gggtgcttc ccgacggga aaacggaag gagagctgg gtgggggag agaagagac agcattttg agcgatccg ccaactgtt caaaagtcg cgatgttcc ggcttcttc cgcaggatg ttctgtttg gacaaatgc cgcaaaaaa acgccgccc aaaatcgag gaaacgcaa gaaagtcga agggcttcc atgatgagc aaaaagaag agtctaacg ccttcagtc cccgttgag gacaccaaa ccaaaactt gatctcagt ttggtggaa cacacgtcc cacatgaaa gcggcaatt cccgttctt ccagtttgt ttagcttgg ttctgtctt gtcatgaac tgcatcgcg cccgggaca gggaccgtt ttcagcgga atgttctgc ttatgcatc ggcaagccg cggttttcg cagaaagac ggcccccgg ccccgcatc ggtgccttc atcatcgac ctcatcatc ggcttcggc cagtttttc accgttttg ttttgtctg gtcgggtgg ggttggtcc atttggtgg ggcgtgatt atggtgaaa cttgccaaa aaaaataaa aaaattaaa gccttagaa gcaaacgcg caagaaggg tccagacaa gtgccagcc gccaatcag aaccaccga gatccggaa agggggtct tgaacagcg gctaaagac atatcactt cttctggaa ggttttttc tacaatatt ttgtacagg ctttcgcac aaattacaa aactttcga tttagcccc aaattgtta tcgttaaac ccatttttg ctggtcaaa aatgttttg taagaagtg tagatagga acaaaatta cgcaagtta cactatttt ttgtaaact aacatgtag aaatgtgta ttatacatt ttatagcgc aggtggggg ttgttactt aacatgtat ttggacaaa gtgtacaaa gctttttca aagtattta aacacttta ttatgaata caataacaa tgcaatacc agctaagta caagcacac agttacaga ttacagaag tatttttct ataaagtta ggctaaatt ggcgtgttt aaatacttt atgtacact tttttaata aagatttat tgtaaataa aacgcgttt ttatttcaa agtacttac cagctaggt gtcttgaat tttacctca tttttattg gatcatggg atagggggc cggaagtgg ctgtgagga ttgatctta cacttcaaa aacacatta attggtaat taatttact tatttcagc gatgaaata taacttatt tacattatc gaaaaaacg aattgactt tttagtaat actgaacct agccattct atccttgta gatggtatg ttccaatat gtacataat tttttaaaa ttcaatatg aaaaactgt cttgggaac aatacatgt acaaaaata aaatgccag aaaatattt ggcatacaa ttacgcgta tattataat actttgaat aatttaaat atatacaca cagcgtcgg aaaatacat acatttact taacattac ttgtgtatt atgatgttt cagcttgtc atgtctgta cgcatgttg atgtatttt tgaacgtgt aagcattgt aggtatggt gtgttttgt ctccctctg tgatattaa ataaatttt aaaaaataa tattcgttc gatatcgaa acacaaagg gaaaacttt cgatcatta ggtttcgtt gcccctccc gcgtaaatc ccag
- >TC034592 RC
ttcctgagc tttcgcagg gctcaaaaa atcattttg ggcactatg aacaaaaat tcgaagcgg gaattgtga ctaagtggt gacggcaga ttaaggctc caacatggc tgggagcac accaactgg gagaatatg aagatcagg gcaaagtcg tagcaattt ttagttttt ttcgttcca aaaatgcgt tcgcccgaa cacagaaac acgtacaat agaacggga cacactcaa gatgaagta tcttcacgt cataaggca ccaggagct tttaccata ctcccaccc gatgatgcg gcccctcaa gaccctcct atggctcct cagccatac aggcttact ccatcctcc cgaagacac aagaacatg tctttcgcg atcctggcg gagcccagc agcttgccc acctcgcgg agccccagc cccaccggc cgcgttagc cccttccgg ggccggggc ttcaacccc atcggctcg cggcacgcg tccccggcc ccgtcaccg ccgccccag ggggctttc accctccag gagtccccc agccactcc aaaatcccg cgactgtcc cgccgcgga agcgccgcc gctttcggg gagaaaatc accgattac caatcgtcc cccaacagg agaaaattg aacgcacag cttagtcaa agcctaact aacattgcg cccagacgg gtcaccgcc cggccgccg ccgagcccc cgaaggccc ccagggtac tccaaaaac accttccag aggctttcc cccatcgta ggtagttcg cctgagcca agtcaatcc cagtcttcc cctaaactc aacactagt agtacagtg agcaagagt cgctcgttt gtaaataga ccgccgagg aatgcctcc aagcccacc tccaaggcc accagtagg gagccgagt cctagcaag aacccagct tcgcccacg aggatcccg gtcaagaat tacagaaat gtgcaagcg aaagttaat agttttagt aggactaaa ccaaaagtg cctcctaaa ccccaagtg ctgagcgag accgattcg gatagtgtg gctaataga agaaaagtg gaacgaaaa gaaagtgtt agtaggtta aggacaaac tctagaatc aatctaaat cttaataac aataataat acgaaaaac aataataaa aacaataaa aataacgta ggtaaagac actaacggg aaccagaaa agtaataat aacaacaat aataaaaat gctgtaaca aataaaggt gccgaatcg agtaaagag aaaaatgaa acgaaggca acaaacgac aaagggaac aataaaaaa gaagactcc accaagaag aatgaggca aaagaagaa acggccaaa aaatcagaa aaagaaagt ccggtgaaa ctgtccgac ttgctccaa ccctcgacg acagctgtg gtttcaagc accacaacc acagtaacc caacccttg aaaatcgaa gccaaaatc gaagaacct gcaaaaacc gacaataaa atggcaccc atggtcgac ggccgcatc ttgagtgca accagcgtc tcccatgcg atgaataaa atgaacgat acggtcctt gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tcaaccaaa ccctctaga caccaaant gactctcaa accctgatg aaggaatcc ggacttacg aaactatca ccggctgca agcaccatc atcgcgatg tccaaccaa aaccctcta gacaccaaa aatgcgacc acggctctg ccgaaagtc gaagaagcc acaactaag accgtggac agtataaac agtaaccac gttacttcg actggaatc gtgccagcg agggttttg gagcaacac gtccagaac aacatgagc aaattgata agtccggac cacaaaatc gggaattcc gtccagaaa accgccaac gataggata atcgaagca cggactgtc gtcgcccat gatgtcaag cccatcaag attactgtg agggagaaa cccatggat gtggaggta cagagcggg aatatccgc ttcgacacg tccaacggc ttgggggag aggccgggt ggaatatcg ggtgaactt gtgcgtgtg aaaaagctg attttcaat agaatgacc cgagagagg gaaagttca agggagcag attgcgaaa atgacgtac atctacgtt atgccctta catgaggag tataatggt tacggagtt ctggaaaat ggaggttta ccccctcct cagcagccc aacgactcg caagaaccc caagaacca cccaaaggc aatgcatgc acgcgattt ctttcaaca tgcaaaaag aaaatgtca tgtaagcga tgcaagaaa caaaaagac gaaactaaa gacgagctc aagaaagaa cgcgaagaa actggaaaa acgggatgc ttcaactgc aggaagaag aaacctgac gtcaccatc aacattgag aatgacgaa cccaagccg aaattttgg gaccggatg aagtgctgc aagagaaac aaggttggc gacactggg tgcttcccg acgggaaaa cggaaggag agctgggtg ggggagaga agagacagc attttgagc gatccgcca actgttcaa aagtcgcga tgttccggc ttcttccgc aggatgttc tgtttggac aaatgccgc aaaaaaacg ccgcccaaa atcgaggaa acgcaagaa agtcgaagg gcttccatg atgagcaaa aagaagagt ctaacgcct tcagtcccc gttgaggac accaaacca aaacttgat ctcagtttg gtggaacac acgtcccac atgaaagcg gcaattccc gttcttcca gtttgttta gcttggttc tgtcttgtc atgaactgc atcgcgccc gggacaggg accgttttc agcggaatg ttctgctta tgcatcggc aagccgcgg ttttcgcag aaagacggc ccccggccc cgcatcggt gccttcatc atcgacctc atcatcggc ttcggccag tttttcacc gttttgttt tgtctggtc gggtggggt tggtccatt tggtggggc gtgattatg gtgaaactt gccaaaaaa aataaaaaa attaaagcc ttagaagca aacgcgcaa gaagggtcc agacaagtg ccagccgcc aatcagaac caccgagat ccggaaagg gggtcttga acagcggct aaagacata tcacttctt ctggaaggt tttttctac aatattttg tacaggctt tcgcacaaa ttacaaaac tttcgattt agccccaaa ttgttatcg ttaaaccca tttttgctg gtcaaaaat gttttgtaa gaagtgtag ataggaaca aaattacgc aagttacac tattttttg taaactaac atgtagaaa tgtgtatta tacatttta tagcgcagg tgggggttg ttacttaac atgtatttg gacaaagtg tacaaagct ttttcaaag tatttaaac actttatta tgaatacaa taacaatgc aataccagc taagtacaa gcacacagt tacagatta cagaagtat ttttctata aagttaggc taaattggc gtgtttaaa tactttatg tacactttt ttaataaag atttattgt aaataaaac gcgttttta tttcaaagt acttaccag ctaggtgtc ttgaatttt acctcattt ttattggat catgggata gggggccgg aagtggctg tgaggattg atcttacac ttcaaaaac acattaatt ggtaattaa tttacttat ttcagcgat gaaatataa cttatttac attatcgaa aaaacgaat tgacttttt agtaatact gaacctagc cattctatc cttgtagat ggtatgttc caatatgta cataatttt ttaaaattc aatatgaaa aactgtctt gggaacaat acatgtaca aaaataaaa tgccagaaa atatttggc atacaatta cgcgtatat tataatact ttgaataat ttaaatata tacacacag cgtcggaaa atacataca tttacttaa cattacttg tgtattatg atgtttcag cttgtcatg tctgtacgc atgttgatg tatttttga acgtgtaag cattgtagg tatggtgtg ttttgtctc cctctgtga tattaaata aattttaaa aaataatat tcgttcgat atcgaaaca caaagggaa aactttcga tcattaggt ttcgttgcc cctcccgcg taaatccca g
