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Gene information
General information
TC003562 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7535939..7541373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003562
    iBeetle screen iB_03579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003562 RA
      MADSDDEYD RKRRDKFRG ERAEATYRG AERVARPRD 
      DWAERDSWS RPRPREYRP GVRDRGYSP SLDPAPKRL 
      RHDYYADSY YNHYSPYHQ SSHREPPPS SQSEGQQPP 
      MMSFKAFLA TQDDNISDS EAIEKYNDY KLEFQRQQL 
      NEFFVAHKD DEWFRLKYH PEDSVKRKE EQMAALKKR 
      VEVFLDLMN NGKLAVSVD CSKTNDLLK LLDTVVIKL 
      EGGTEEDLA VLEQEYAEL EEKNKEKGK EEVVEKPND 
      DTPKKEQPA EKPKPDEPE AKENGETTA EDGEIEQEK 
      KEEEEKPAP EENKEPEKM EVVEEEKKE SENPEPAKE 
      NEPEEGEEV KKEPKDKKR KRSYSGSSS GSSSSSSDS 
      EDEKEKKEK KEEEDEETE KDKPKEEVK EHIETVEDD 
      KPEKPKSLH KTTSIFLRN LAPTITKQE VEAVCGRYE 
      GFLRVALAD PQPERRWLR RGWVTFKRD ANIKEICWN 
      LNNIRLRDC ELGAIVNRD LSRRIRPVN GITAHKQVV 
      RSDIRISAK VALHLDNKV GLWLDDEKK DKPQQTFGL 
      VSNNPVLHN ITDYLIEEA SAEEEELLG LEPTAETQD 
      SATTVERDE QLISVLDRI ILYLRVVHS VDYYNHCEY 
      PNEDEMPNR CGILHARGP PPTTKTDMT QFIGAPVEA 
      KMTSFLPEK PRDENKNET KLINLSLKD VDTEIDKFV 
      QANTRELAK DKWLCPLSG KKFKGPDFV RKHIFNKHA 
      EKIEEVKKE VEFFNNYLR DPKRPMLAE APQPKREEP 
      PSYNHPSYG GSYGGYGRL PPYYNQGYS QRSRGYTPR 
      SRGGPGDYR PVIHYRDLD APREPEEFI
    CDS sequence
    • >TC003562 RA
      atggctgat agtgacgat gaatacgac cgcaaacgc 
      cgcgataag ttcagaggt gaacgcgcc gaagccact 
      tacaggggg gccgaacgg gttgccaga cctagggac 
      gactgggcc gaacgtgac tcttggtca cgacctagg 
      cctcgggag taccgcccg ggggtgaga gacagggga 
      tactcgccg agtctggac ccagccccc aagaggctg 
      cgccacgat tactatgct gatagctac tacaaccat 
      tactcgccc tatcaccaa agctcgcac agagaacct 
      ccgccatcg tctcagtct gagggtcag cagccgcca 
      atgatgtcc tttaaggcg tttctggcc acccaggac 
      gataacatt tccgatagt gaagctatc gaaaaatac 
      aatgactac aaattggag ttccagcga cagcaactt 
      aacgaattt tttgttgcc cataaagac gacgaatgg 
      ttccgtttg aagtaccat cctgaagat tcagtaaaa 
      cgaaaagaa gaacaaatg gcagcattg aagaaaaga 
      gtcgaggtt ttcttggac ttgatgaac aacggaaaa 
      cttgcggtt tcagttgat tgttcaaaa acgaatgac 
      ttacttaaa ctgttagat actgtcgtt attaaactc 
      gaaggtggc actgaggaa gacttggcc gttttggag 
      caggagtat gccgaattg gaagagaaa aacaaagaa 
      aagggtaaa gaagaagtc gtcgaaaaa ccaaacgat 
      gacactccc aagaaagaa caacctgcg gaaaagcca 
      aagcctgat gaacctgaa gcaaaagaa aacggcgaa 
      acgactgca gaagacggt gaaatagaa caagagaag 
      aaggaagag gaggaaaaa ccagctcct gaagaaaac 
      aaagagcct gaaaaaatg gaggtggta gaagaagag 
      aagaaagag tctgaaaac cccgagccg gcgaaagaa 
      aacgaacca gaagaaggc gaagaagtg aagaaagaa 
      cctaaagac aagaaacgc aagcgttcc tattcggga 
      agcagcagc ggctcgtcg agtagcagc agcgacagc 
      gaagacgag aaagagaaa aaagagaag aaggaagag 
      gaggacgaa gaaaccgaa aaagacaaa ccgaaagaa 
      gaagtcaaa gagcacatt gaaaccgtc gaagacgac 
      aaaccggaa aaacccaaa tcgctgcac aaaaccacg 
      tcaattttt ctgcgtaat ttagcgccg actattacg 
      aaacaagaa gtggaggcg gtttgcggc cgctatgag 
      ggttttctg cgcgtggct ttggccgac cctcagccg 
      gagcggcgg tggttgagg cgcggttgg gtcacgttt 
      aaacgagac gcgaatatt aaggagatc tgttggaat 
      ttgaataat attagactg agggactgc gaattgggg 
      gcgatcgtt aatcgggat ttgagtcgg aggattcgg 
      cctgttaac ggcattacg gcccataaa caagtcgtc 
      cggtccgat attcgaata tcggcgaaa gttgcgctg 
      catctcgat aataaagtt gggctttgg ttggacgat 
      gagaagaaa gataagccc caacagacg ttcgggctg 
      gtgtcgaat aatcctgtt ctgcacaat attaccgac 
      tatctgatc gaggaggcg agtgccgag gaggaggaa 
      cttctgggg ctggagcca acggcggag acgcaagat 
      tcggccact actgttgaa agagacgaa caacttatt 
      tcagtcttg gaccggatt attctgtat ttgcgtgtt 
      gttcactcg gttgattat tacaatcac tgcgagtat 
      cccaatgag gatgagatg ccaaatagg tgcgggatt 
      ctgcacgcc agggggccg ccacctacc accaaaact 
      gatatgacc caatttatt ggggctcca gtcgaggcc 
      aaaatgact agttttctt cccgaaaaa ccccgcgac 
      gagaacaaa aacgaaacg aaactaatc aacctgtct 
      ctaaaagac gttgatacc gaaattgac aagttcgtt 
      caagcgaat acaagagag ttggccaag gacaagtgg 
      ttgtgtcca ttgtccggc aaaaaattc aagggtcct 
      gacttcgtc cgtaagcac atcttcaac aagcatgct 
      gaaaaaatt gaagaggtg aagaaggaa gtcgaattt 
      ttcaataac tatttacgg gaccctaaa agaccaatg 
      ttggcggaa gccccccag ccaaaacgt gaggaaccg 
      ccctcttac aaccacccg tcgtatgga ggttcctat 
      ggtgggtat ggtaggtta cccccatat tacaatcaa 
      gggtattcg caacgttct aggggttat acaccaaga 
      tcaaggggt ggtcctggg gattatagg ccggttatt 
      cactaccgg gacctcgat gctccaagg gagccagaa 
      gagtttatt taa
    mRNA sequence
    • >TC003562 RA
      ctgcaaaac ccccacggc gtctttgac gtgtcggcc 
      ataattttg tatcaactt cctcaagag aaatccgaa 
      ttttcgccg gaaaatcgc caaaagtat tatttttag 
      aacagaaag aaatggctg atagtgacg atgaatacg 
      accgcaaac gccgcgata agttcagag gtgaacgcg 
      ccgaagcca cttacaggg gggccgaac gggttgcca 
      gacctaggg acgactggg ccgaacgtg actcttggt 
      cacgaccta ggcctcggg agtaccgcc cgggggtga 
      gagacaggg gatactcgc cgagtctgg acccagccc 
      ccaagaggc tgcgccacg attactatg ctgatagct 
      actacaacc attactcgc cctatcacc aaagctcgc 
      acagagaac ctccgccat cgtctcagt ctgagggtc 
      agcagccgc caatgatgt cctttaagg cgtttctgg 
      ccacccagg acgataaca tttccgata gtgaagcta 
      tcgaaaaat acaatgact acaaattgg agttccagc 
      gacagcaac ttaacgaat tttttgttg cccataaag 
      acgacgaat ggttccgtt tgaagtacc atcctgaag 
      attcagtaa aacgaaaag aagaacaaa tggcagcat 
      tgaagaaaa gagtcgagg ttttcttgg acttgatga 
      acaacggaa aacttgcgg tttcagttg attgttcaa 
      aaacgaatg acttactta aactgttag atactgtcg 
      ttattaaac tcgaaggtg gcactgagg aagacttgg 
      ccgttttgg agcaggagt atgccgaat tggaagaga 
      aaaacaaag aaaagggta aagaagaag tcgtcgaaa 
      aaccaaacg atgacactc ccaagaaag aacaacctg 
      cggaaaagc caaagcctg atgaacctg aagcaaaag 
      aaaacggcg aaacgactg cagaagacg gtgaaatag 
      aacaagaga agaaggaag aggaggaaa aaccagctc 
      ctgaagaaa acaaagagc ctgaaaaaa tggaggtgg 
      tagaagaag agaagaaag agtctgaaa accccgagc 
      cggcgaaag aaaacgaac cagaagaag gcgaagaag 
      tgaagaaag aacctaaag acaagaaac gcaagcgtt 
      cctattcgg gaagcagca gcggctcgt cgagtagca 
      gcagcgaca gcgaagacg agaaagaga aaaaagaga 
      agaaggaag aggaggacg aagaaaccg aaaaagaca 
      aaccgaaag aagaagtca aagagcaca ttgaaaccg 
      tcgaagacg acaaaccgg aaaaaccca aatcgctgc 
      acaaaacca cgtcaattt ttctgcgta atttagcgc 
      cgactatta cgaaacaag aagtggagg cggtttgcg 
      gccgctatg agggttttc tgcgcgtgg ctttggccg 
      accctcagc cggagcggc ggtggttga ggcgcggtt 
      gggtcacgt ttaaacgag acgcgaata ttaaggaga 
      tctgttgga atttgaata atattagac tgagggact 
      gcgaattgg gggcgatcg ttaatcggg atttgagtc 
      ggaggattc ggcctgtta acggcatta cggcccata 
      aacaagtcg tccggtccg atattcgaa tatcggcga 
      aagttgcgc tgcatctcg ataataaag ttgggcttt 
      ggttggacg atgagaaga aagataagc cccaacaga 
      cgttcgggc tggtgtcga ataatcctg ttctgcaca 
      atattaccg actatctga tcgaggagg cgagtgccg 
      aggaggagg aacttctgg ggctggagc caacggcgg 
      agacgcaag attcggcca ctactgttg aaagagacg 
      aacaactta tttcagtct tggaccgga ttattctgt 
      atttgcgtg ttgttcact cggttgatt attacaatc 
      actgcgagt atcccaatg aggatgaga tgccaaata 
      ggtgcggga ttctgcacg ccagggggc cgccaccta 
      ccaccaaaa ctgatatga cccaattta ttggggctc 
      cagtcgagg ccaaaatga ctagttttc ttcccgaaa 
      aaccccgcg acgagaaca aaaacgaaa cgaaactaa 
      tcaacctgt ctctaaaag acgttgata ccgaaattg 
      acaagttcg ttcaagcga atacaagag agttggcca 
      aggacaagt ggttgtgtc cattgtccg gcaaaaaat 
      tcaagggtc ctgacttcg tccgtaagc acatcttca 
      acaagcatg ctgaaaaaa ttgaagagg tgaagaagg 
      aagtcgaat ttttcaata actatttac gggacccta 
      aaagaccaa tgttggcgg aagcccccc agccaaaac 
      gtgaggaac cgccctctt acaaccacc cgtcgtatg 
      gaggttcct atggtgggt atggtaggt tacccccat 
      attacaatc aagggtatt cgcaacgtt ctaggggtt 
      atacaccaa gatcaaggg gtggtcctg gggattata 
      ggccggtta ttcactacc gggacctcg atgctccaa 
      gggagccag aagagttta tttaaatct ttaatttat 
      gcttgtaag ttagaggac taagaaaga atatttcaa 
      acaacattt tattttgat aataagttt gtaaaggta 
      ccgtcattc aatcaataa actgttcgt attttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY