| General information | |||
| TC003562 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 7535939..7541373 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003562 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03579 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC003562 RA
MADSDDEYD RKRRDKFRG ERAEATYRG AERVARPRD DWAERDSWS RPRPREYRP GVRDRGYSP SLDPAPKRL RHDYYADSY YNHYSPYHQ SSHREPPPS SQSEGQQPP MMSFKAFLA TQDDNISDS EAIEKYNDY KLEFQRQQL NEFFVAHKD DEWFRLKYH PEDSVKRKE EQMAALKKR VEVFLDLMN NGKLAVSVD CSKTNDLLK LLDTVVIKL EGGTEEDLA VLEQEYAEL EEKNKEKGK EEVVEKPND DTPKKEQPA EKPKPDEPE AKENGETTA EDGEIEQEK KEEEEKPAP EENKEPEKM EVVEEEKKE SENPEPAKE NEPEEGEEV KKEPKDKKR KRSYSGSSS GSSSSSSDS EDEKEKKEK KEEEDEETE KDKPKEEVK EHIETVEDD KPEKPKSLH KTTSIFLRN LAPTITKQE VEAVCGRYE GFLRVALAD PQPERRWLR RGWVTFKRD ANIKEICWN LNNIRLRDC ELGAIVNRD LSRRIRPVN GITAHKQVV RSDIRISAK VALHLDNKV GLWLDDEKK DKPQQTFGL VSNNPVLHN ITDYLIEEA SAEEEELLG LEPTAETQD SATTVERDE QLISVLDRI ILYLRVVHS VDYYNHCEY PNEDEMPNR CGILHARGP PPTTKTDMT QFIGAPVEA KMTSFLPEK PRDENKNET KLINLSLKD VDTEIDKFV QANTRELAK DKWLCPLSG KKFKGPDFV RKHIFNKHA EKIEEVKKE VEFFNNYLR DPKRPMLAE APQPKREEP PSYNHPSYG GSYGGYGRL PPYYNQGYS QRSRGYTPR SRGGPGDYR PVIHYRDLD APREPEEFI
- >TC003562 RA
atggctgat agtgacgat gaatacgac cgcaaacgc cgcgataag ttcagaggt gaacgcgcc gaagccact tacaggggg gccgaacgg gttgccaga cctagggac gactgggcc gaacgtgac tcttggtca cgacctagg cctcgggag taccgcccg ggggtgaga gacagggga tactcgccg agtctggac ccagccccc aagaggctg cgccacgat tactatgct gatagctac tacaaccat tactcgccc tatcaccaa agctcgcac agagaacct ccgccatcg tctcagtct gagggtcag cagccgcca atgatgtcc tttaaggcg tttctggcc acccaggac gataacatt tccgatagt gaagctatc gaaaaatac aatgactac aaattggag ttccagcga cagcaactt aacgaattt tttgttgcc cataaagac gacgaatgg ttccgtttg aagtaccat cctgaagat tcagtaaaa cgaaaagaa gaacaaatg gcagcattg aagaaaaga gtcgaggtt ttcttggac ttgatgaac aacggaaaa cttgcggtt tcagttgat tgttcaaaa acgaatgac ttacttaaa ctgttagat actgtcgtt attaaactc gaaggtggc actgaggaa gacttggcc gttttggag caggagtat gccgaattg gaagagaaa aacaaagaa aagggtaaa gaagaagtc gtcgaaaaa ccaaacgat gacactccc aagaaagaa caacctgcg gaaaagcca aagcctgat gaacctgaa gcaaaagaa aacggcgaa acgactgca gaagacggt gaaatagaa caagagaag aaggaagag gaggaaaaa ccagctcct gaagaaaac aaagagcct gaaaaaatg gaggtggta gaagaagag aagaaagag tctgaaaac cccgagccg gcgaaagaa aacgaacca gaagaaggc gaagaagtg aagaaagaa cctaaagac aagaaacgc aagcgttcc tattcggga agcagcagc ggctcgtcg agtagcagc agcgacagc gaagacgag aaagagaaa aaagagaag aaggaagag gaggacgaa gaaaccgaa aaagacaaa ccgaaagaa gaagtcaaa gagcacatt gaaaccgtc gaagacgac aaaccggaa aaacccaaa tcgctgcac aaaaccacg tcaattttt ctgcgtaat ttagcgccg actattacg aaacaagaa gtggaggcg gtttgcggc cgctatgag ggttttctg cgcgtggct ttggccgac cctcagccg gagcggcgg tggttgagg cgcggttgg gtcacgttt aaacgagac gcgaatatt aaggagatc tgttggaat ttgaataat attagactg agggactgc gaattgggg gcgatcgtt aatcgggat ttgagtcgg aggattcgg cctgttaac ggcattacg gcccataaa caagtcgtc cggtccgat attcgaata tcggcgaaa gttgcgctg catctcgat aataaagtt gggctttgg ttggacgat gagaagaaa gataagccc caacagacg ttcgggctg gtgtcgaat aatcctgtt ctgcacaat attaccgac tatctgatc gaggaggcg agtgccgag gaggaggaa cttctgggg ctggagcca acggcggag acgcaagat tcggccact actgttgaa agagacgaa caacttatt tcagtcttg gaccggatt attctgtat ttgcgtgtt gttcactcg gttgattat tacaatcac tgcgagtat cccaatgag gatgagatg ccaaatagg tgcgggatt ctgcacgcc agggggccg ccacctacc accaaaact gatatgacc caatttatt ggggctcca gtcgaggcc aaaatgact agttttctt cccgaaaaa ccccgcgac gagaacaaa aacgaaacg aaactaatc aacctgtct ctaaaagac gttgatacc gaaattgac aagttcgtt caagcgaat acaagagag ttggccaag gacaagtgg ttgtgtcca ttgtccggc aaaaaattc aagggtcct gacttcgtc cgtaagcac atcttcaac aagcatgct gaaaaaatt gaagaggtg aagaaggaa gtcgaattt ttcaataac tatttacgg gaccctaaa agaccaatg ttggcggaa gccccccag ccaaaacgt gaggaaccg ccctcttac aaccacccg tcgtatgga ggttcctat ggtgggtat ggtaggtta cccccatat tacaatcaa gggtattcg caacgttct aggggttat acaccaaga tcaaggggt ggtcctggg gattatagg ccggttatt cactaccgg gacctcgat gctccaagg gagccagaa gagtttatt taa
- >TC003562 RA
ctgcaaaac ccccacggc gtctttgac gtgtcggcc ataattttg tatcaactt cctcaagag aaatccgaa ttttcgccg gaaaatcgc caaaagtat tatttttag aacagaaag aaatggctg atagtgacg atgaatacg accgcaaac gccgcgata agttcagag gtgaacgcg ccgaagcca cttacaggg gggccgaac gggttgcca gacctaggg acgactggg ccgaacgtg actcttggt cacgaccta ggcctcggg agtaccgcc cgggggtga gagacaggg gatactcgc cgagtctgg acccagccc ccaagaggc tgcgccacg attactatg ctgatagct actacaacc attactcgc cctatcacc aaagctcgc acagagaac ctccgccat cgtctcagt ctgagggtc agcagccgc caatgatgt cctttaagg cgtttctgg ccacccagg acgataaca tttccgata gtgaagcta tcgaaaaat acaatgact acaaattgg agttccagc gacagcaac ttaacgaat tttttgttg cccataaag acgacgaat ggttccgtt tgaagtacc atcctgaag attcagtaa aacgaaaag aagaacaaa tggcagcat tgaagaaaa gagtcgagg ttttcttgg acttgatga acaacggaa aacttgcgg tttcagttg attgttcaa aaacgaatg acttactta aactgttag atactgtcg ttattaaac tcgaaggtg gcactgagg aagacttgg ccgttttgg agcaggagt atgccgaat tggaagaga aaaacaaag aaaagggta aagaagaag tcgtcgaaa aaccaaacg atgacactc ccaagaaag aacaacctg cggaaaagc caaagcctg atgaacctg aagcaaaag aaaacggcg aaacgactg cagaagacg gtgaaatag aacaagaga agaaggaag aggaggaaa aaccagctc ctgaagaaa acaaagagc ctgaaaaaa tggaggtgg tagaagaag agaagaaag agtctgaaa accccgagc cggcgaaag aaaacgaac cagaagaag gcgaagaag tgaagaaag aacctaaag acaagaaac gcaagcgtt cctattcgg gaagcagca gcggctcgt cgagtagca gcagcgaca gcgaagacg agaaagaga aaaaagaga agaaggaag aggaggacg aagaaaccg aaaaagaca aaccgaaag aagaagtca aagagcaca ttgaaaccg tcgaagacg acaaaccgg aaaaaccca aatcgctgc acaaaacca cgtcaattt ttctgcgta atttagcgc cgactatta cgaaacaag aagtggagg cggtttgcg gccgctatg agggttttc tgcgcgtgg ctttggccg accctcagc cggagcggc ggtggttga ggcgcggtt gggtcacgt ttaaacgag acgcgaata ttaaggaga tctgttgga atttgaata atattagac tgagggact gcgaattgg gggcgatcg ttaatcggg atttgagtc ggaggattc ggcctgtta acggcatta cggcccata aacaagtcg tccggtccg atattcgaa tatcggcga aagttgcgc tgcatctcg ataataaag ttgggcttt ggttggacg atgagaaga aagataagc cccaacaga cgttcgggc tggtgtcga ataatcctg ttctgcaca atattaccg actatctga tcgaggagg cgagtgccg aggaggagg aacttctgg ggctggagc caacggcgg agacgcaag attcggcca ctactgttg aaagagacg aacaactta tttcagtct tggaccgga ttattctgt atttgcgtg ttgttcact cggttgatt attacaatc actgcgagt atcccaatg aggatgaga tgccaaata ggtgcggga ttctgcacg ccagggggc cgccaccta ccaccaaaa ctgatatga cccaattta ttggggctc cagtcgagg ccaaaatga ctagttttc ttcccgaaa aaccccgcg acgagaaca aaaacgaaa cgaaactaa tcaacctgt ctctaaaag acgttgata ccgaaattg acaagttcg ttcaagcga atacaagag agttggcca aggacaagt ggttgtgtc cattgtccg gcaaaaaat tcaagggtc ctgacttcg tccgtaagc acatcttca acaagcatg ctgaaaaaa ttgaagagg tgaagaagg aagtcgaat ttttcaata actatttac gggacccta aaagaccaa tgttggcgg aagcccccc agccaaaac gtgaggaac cgccctctt acaaccacc cgtcgtatg gaggttcct atggtgggt atggtaggt tacccccat attacaatc aagggtatt cgcaacgtt ctaggggtt atacaccaa gatcaaggg gtggtcctg gggattata ggccggtta ttcactacc gggacctcg atgctccaa gggagccag aagagttta tttaaatct ttaatttat gcttgtaag ttagaggac taagaaaga atatttcaa acaacattt tattttgat aataagttt gtaaaggta ccgtcattc aatcaataa actgttcgt attttt
