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Gene information
General information
TC003562 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7535939..7541373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003562
    iBeetle screen iB_03579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003562 RA
      MADSDDEYD RKRRDKFRG ERAEATYRG AERVARPRD 
DWAERDSWS RPRPREYRP GVRDRGYSP SLDPAPKRL 
RHDYYADSY YNHYSPYHQ SSHREPPPS SQSEGQQPP 
MMSFKAFLA TQDDNISDS EAIEKYNDY KLEFQRQQL 
NEFFVAHKD DEWFRLKYH PEDSVKRKE EQMAALKKR 
VEVFLDLMN NGKLAVSVD CSKTNDLLK LLDTVVIKL 
EGGTEEDLA VLEQEYAEL EEKNKEKGK EEVVEKPND 
DTPKKEQPA EKPKPDEPE AKENGETTA EDGEIEQEK 
KEEEEKPAP EENKEPEKM EVVEEEKKE SENPEPAKE 
NEPEEGEEV KKEPKDKKR KRSYSGSSS GSSSSSSDS 
EDEKEKKEK KEEEDEETE KDKPKEEVK EHIETVEDD 
KPEKPKSLH KTTSIFLRN LAPTITKQE VEAVCGRYE 
GFLRVALAD PQPERRWLR RGWVTFKRD ANIKEICWN 
LNNIRLRDC ELGAIVNRD LSRRIRPVN GITAHKQVV 
RSDIRISAK VALHLDNKV GLWLDDEKK DKPQQTFGL 
VSNNPVLHN ITDYLIEEA SAEEEELLG LEPTAETQD 
SATTVERDE QLISVLDRI ILYLRVVHS VDYYNHCEY 
PNEDEMPNR CGILHARGP PPTTKTDMT QFIGAPVEA 
KMTSFLPEK PRDENKNET KLINLSLKD VDTEIDKFV 
QANTRELAK DKWLCPLSG KKFKGPDFV RKHIFNKHA 
EKIEEVKKE VEFFNNYLR DPKRPMLAE APQPKREEP 
PSYNHPSYG GSYGGYGRL PPYYNQGYS QRSRGYTPR 
SRGGPGDYR PVIHYRDLD APREPEEFI
    CDS sequence
    • >TC003562 RA
      atggctgat agtgacgat gaatacgac cgcaaacgc 
cgcgataag ttcagaggt gaacgcgcc gaagccact 
tacaggggg gccgaacgg gttgccaga cctagggac 
gactgggcc gaacgtgac tcttggtca cgacctagg 
cctcgggag taccgcccg ggggtgaga gacagggga 
tactcgccg agtctggac ccagccccc aagaggctg 
cgccacgat tactatgct gatagctac tacaaccat 
tactcgccc tatcaccaa agctcgcac agagaacct 
ccgccatcg tctcagtct gagggtcag cagccgcca 
atgatgtcc tttaaggcg tttctggcc acccaggac 
gataacatt tccgatagt gaagctatc gaaaaatac 
aatgactac aaattggag ttccagcga cagcaactt 
aacgaattt tttgttgcc cataaagac gacgaatgg 
ttccgtttg aagtaccat cctgaagat tcagtaaaa 
cgaaaagaa gaacaaatg gcagcattg aagaaaaga 
gtcgaggtt ttcttggac ttgatgaac aacggaaaa 
cttgcggtt tcagttgat tgttcaaaa acgaatgac 
ttacttaaa ctgttagat actgtcgtt attaaactc 
gaaggtggc actgaggaa gacttggcc gttttggag 
caggagtat gccgaattg gaagagaaa aacaaagaa 
aagggtaaa gaagaagtc gtcgaaaaa ccaaacgat 
gacactccc aagaaagaa caacctgcg gaaaagcca 
aagcctgat gaacctgaa gcaaaagaa aacggcgaa 
acgactgca gaagacggt gaaatagaa caagagaag 
aaggaagag gaggaaaaa ccagctcct gaagaaaac 
aaagagcct gaaaaaatg gaggtggta gaagaagag 
aagaaagag tctgaaaac cccgagccg gcgaaagaa 
aacgaacca gaagaaggc gaagaagtg aagaaagaa 
cctaaagac aagaaacgc aagcgttcc tattcggga 
agcagcagc ggctcgtcg agtagcagc agcgacagc 
gaagacgag aaagagaaa aaagagaag aaggaagag 
gaggacgaa gaaaccgaa aaagacaaa ccgaaagaa 
gaagtcaaa gagcacatt gaaaccgtc gaagacgac 
aaaccggaa aaacccaaa tcgctgcac aaaaccacg 
tcaattttt ctgcgtaat ttagcgccg actattacg 
aaacaagaa gtggaggcg gtttgcggc cgctatgag 
ggttttctg cgcgtggct ttggccgac cctcagccg 
gagcggcgg tggttgagg cgcggttgg gtcacgttt 
aaacgagac gcgaatatt aaggagatc tgttggaat 
ttgaataat attagactg agggactgc gaattgggg 
gcgatcgtt aatcgggat ttgagtcgg aggattcgg 
cctgttaac ggcattacg gcccataaa caagtcgtc 
cggtccgat attcgaata tcggcgaaa gttgcgctg 
catctcgat aataaagtt gggctttgg ttggacgat 
gagaagaaa gataagccc caacagacg ttcgggctg 
gtgtcgaat aatcctgtt ctgcacaat attaccgac 
tatctgatc gaggaggcg agtgccgag gaggaggaa 
cttctgggg ctggagcca acggcggag acgcaagat 
tcggccact actgttgaa agagacgaa caacttatt 
tcagtcttg gaccggatt attctgtat ttgcgtgtt 
gttcactcg gttgattat tacaatcac tgcgagtat 
cccaatgag gatgagatg ccaaatagg tgcgggatt 
ctgcacgcc agggggccg ccacctacc accaaaact 
gatatgacc caatttatt ggggctcca gtcgaggcc 
aaaatgact agttttctt cccgaaaaa ccccgcgac 
gagaacaaa aacgaaacg aaactaatc aacctgtct 
ctaaaagac gttgatacc gaaattgac aagttcgtt 
caagcgaat acaagagag ttggccaag gacaagtgg 
ttgtgtcca ttgtccggc aaaaaattc aagggtcct 
gacttcgtc cgtaagcac atcttcaac aagcatgct 
gaaaaaatt gaagaggtg aagaaggaa gtcgaattt 
ttcaataac tatttacgg gaccctaaa agaccaatg 
ttggcggaa gccccccag ccaaaacgt gaggaaccg 
ccctcttac aaccacccg tcgtatgga ggttcctat 
ggtgggtat ggtaggtta cccccatat tacaatcaa 
gggtattcg caacgttct aggggttat acaccaaga 
tcaaggggt ggtcctggg gattatagg ccggttatt 
cactaccgg gacctcgat gctccaagg gagccagaa 
gagtttatt taa
    mRNA sequence
    • >TC003562 RA
      ctgcaaaac ccccacggc gtctttgac gtgtcggcc 
ataattttg tatcaactt cctcaagag aaatccgaa 
ttttcgccg gaaaatcgc caaaagtat tatttttag 
aacagaaag aaatggctg atagtgacg atgaatacg 
accgcaaac gccgcgata agttcagag gtgaacgcg 
ccgaagcca cttacaggg gggccgaac gggttgcca 
gacctaggg acgactggg ccgaacgtg actcttggt 
cacgaccta ggcctcggg agtaccgcc cgggggtga 
gagacaggg gatactcgc cgagtctgg acccagccc 
ccaagaggc tgcgccacg attactatg ctgatagct 
actacaacc attactcgc cctatcacc aaagctcgc 
acagagaac ctccgccat cgtctcagt ctgagggtc 
agcagccgc caatgatgt cctttaagg cgtttctgg 
ccacccagg acgataaca tttccgata gtgaagcta 
tcgaaaaat acaatgact acaaattgg agttccagc 
gacagcaac ttaacgaat tttttgttg cccataaag 
acgacgaat ggttccgtt tgaagtacc atcctgaag 
attcagtaa aacgaaaag aagaacaaa tggcagcat 
tgaagaaaa gagtcgagg ttttcttgg acttgatga 
acaacggaa aacttgcgg tttcagttg attgttcaa 
aaacgaatg acttactta aactgttag atactgtcg 
ttattaaac tcgaaggtg gcactgagg aagacttgg 
ccgttttgg agcaggagt atgccgaat tggaagaga 
aaaacaaag aaaagggta aagaagaag tcgtcgaaa 
aaccaaacg atgacactc ccaagaaag aacaacctg 
cggaaaagc caaagcctg atgaacctg aagcaaaag 
aaaacggcg aaacgactg cagaagacg gtgaaatag 
aacaagaga agaaggaag aggaggaaa aaccagctc 
ctgaagaaa acaaagagc ctgaaaaaa tggaggtgg 
tagaagaag agaagaaag agtctgaaa accccgagc 
cggcgaaag aaaacgaac cagaagaag gcgaagaag 
tgaagaaag aacctaaag acaagaaac gcaagcgtt 
cctattcgg gaagcagca gcggctcgt cgagtagca 
gcagcgaca gcgaagacg agaaagaga aaaaagaga 
agaaggaag aggaggacg aagaaaccg aaaaagaca 
aaccgaaag aagaagtca aagagcaca ttgaaaccg 
tcgaagacg acaaaccgg aaaaaccca aatcgctgc 
acaaaacca cgtcaattt ttctgcgta atttagcgc 
cgactatta cgaaacaag aagtggagg cggtttgcg 
gccgctatg agggttttc tgcgcgtgg ctttggccg 
accctcagc cggagcggc ggtggttga ggcgcggtt 
gggtcacgt ttaaacgag acgcgaata ttaaggaga 
tctgttgga atttgaata atattagac tgagggact 
gcgaattgg gggcgatcg ttaatcggg atttgagtc 
ggaggattc ggcctgtta acggcatta cggcccata 
aacaagtcg tccggtccg atattcgaa tatcggcga 
aagttgcgc tgcatctcg ataataaag ttgggcttt 
ggttggacg atgagaaga aagataagc cccaacaga 
cgttcgggc tggtgtcga ataatcctg ttctgcaca 
atattaccg actatctga tcgaggagg cgagtgccg 
aggaggagg aacttctgg ggctggagc caacggcgg 
agacgcaag attcggcca ctactgttg aaagagacg 
aacaactta tttcagtct tggaccgga ttattctgt 
atttgcgtg ttgttcact cggttgatt attacaatc 
actgcgagt atcccaatg aggatgaga tgccaaata 
ggtgcggga ttctgcacg ccagggggc cgccaccta 
ccaccaaaa ctgatatga cccaattta ttggggctc 
cagtcgagg ccaaaatga ctagttttc ttcccgaaa 
aaccccgcg acgagaaca aaaacgaaa cgaaactaa 
tcaacctgt ctctaaaag acgttgata ccgaaattg 
acaagttcg ttcaagcga atacaagag agttggcca 
aggacaagt ggttgtgtc cattgtccg gcaaaaaat 
tcaagggtc ctgacttcg tccgtaagc acatcttca 
acaagcatg ctgaaaaaa ttgaagagg tgaagaagg 
aagtcgaat ttttcaata actatttac gggacccta 
aaagaccaa tgttggcgg aagcccccc agccaaaac 
gtgaggaac cgccctctt acaaccacc cgtcgtatg 
gaggttcct atggtgggt atggtaggt tacccccat 
attacaatc aagggtatt cgcaacgtt ctaggggtt 
atacaccaa gatcaaggg gtggtcctg gggattata 
ggccggtta ttcactacc gggacctcg atgctccaa 
gggagccag aagagttta tttaaatct ttaatttat 
gcttgtaag ttagaggac taagaaaga atatttcaa 
acaacattt tattttgat aataagttt gtaaaggta 
ccgtcattc aatcaataa actgttcgt attttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY