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Gene information
General information
TC003554 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2678036..2681971
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003554
    iBeetle screen iB_03576
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection



    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003554 RA
      MTAIESPDF IAINNTSSP TDDFVTTKS VRIAEMARL 
      STPKPIMYL GSTTEFQRQ GTGIPKTPY KPGTGYRAG 
      TGFKPTTNF KQTSFEKPG TGYKPGTGF RPGTGADVV 
      NRPMTAVRG AGYTSHGKP FDPLNQAAS APTPPLELQ 
      KDDSPEQKI RQQKAKIMQ LVEEACVAE CEGDYRKSL 
      SKAKEASNK ERVLIKMQE QSDLSDLHD IDLTFVVLF 
      TLGNQYAAN ELYTEALST YQMIIKNRM FSNAHRLKI 
      NMGNIYFKQ GQFQMAVKM YRMALDQVS SSQKNLRIK 
      IMHNIALVF IKMGQWEEA ISSLEYIMG EQACHRAGL 
      HLIICCRAL EDRDRMKTA FSMLLNVPL EVEDEEKYN 
      LEQDNPEDV LIAMAIQND DLHKYEIKK CKDAEYCIL 
      TAAKLIAPL IEDTFSLGY DWCVMSIKN SEYARLASN 
      LEINKAVMF LKQNQLPEA IDTLKAFEK DSVIATNAA 
      INLSFIYYL QGDIENATK YAQVVETNP TKSADGYVN 
      LGACLMAKG QPEDAINSF KNALELDPT HFEAIYNLG 
      LVLKRQGHY AEALQCFQR FSGSLALLP NVVYQVANL 
      LELKGDSEA AADMYQQLL GLVPTDAGA LQKMGELYD 
      HDGDKQQAH HYHIESFRY YPANLSVIN WLGSYYIEM 
      QVVERALVY FEKAALMQP NEPKWNMMV AGCHRRSGN 
      MHRALTLYQ EIHRKFPEN VECLRFLVR LCNDMGMRE 
      AQDYILELK KIEKAKEVR ERVNSSRPS SRRSNSGLS 
      SRAGSGFSP VPEHGPLTS ANRNLRSSR LIRAHNSAE 
      STDSGFGQP MIDASYSDP LGPQPLRPR TGAGKPLDF 
      DDFANDELG DDLLPE
    CDS sequence
    • >TC003554 RA
      atgaccgca atagagtcg cctgatttc attgcaatc 
      aacaacacg tcatcccca accgacgat tttgtaaca 
      accaaaagt gtaagaatc gccgaaatg gccagatta 
      tcaacccct aagcccata atgtacctg ggatcaacc 
      acagagttt caaagacaa ggaaccggg attcccaag 
      accccctac aagcctgga acgggatac agggccggg 
      actgggttc aaacccacg actaacttt aagcagacg 
      agttttgaa aaaccggga actgggtat aaacccgga 
      actgggttc agaccaggg actggggcc gatgtggtg 
      aacaggccc atgacggcg gtgcgcggg gctgggtac 
      acctcccat gggaaacct ttcgaccct ttgaaccaa 
      gctgcttcg gccccaacg ccgcctctg gagttgcaa 
      aaggatgac tctcccgaa cagaaaatt cggcaacaa 
      aaggcaaaa atcatgcag cttgtggaa gaggcgtgt 
      gtcgcggaa tgcgagggt gactatcgg aagtcgctc 
      tctaaggct aaagaagcg tcaaataag gagcgggtt 
      ttgatcaaa atgcaggaa cagtctgat ctgagtgac 
      ttgcacgat attgattta acttttgtt gttttgttc 
      acacttggt aaccagtac gcagccaat gaattatac 
      acggaagca ctcagcacg tatcaaatg atcataaaa 
      aatcgaatg ttttcaaat gcacaccgt ttgaagata 
      aatatgggc aatatttat ttcaaacaa ggccagttc 
      caaatggcg gtcaaaatg tatcgcatg gctttggac 
      caagtgtct tcaagtcaa aaaaatctg agaatcaaa 
      ataatgcac aatattgcg ttagttttt atcaaaatg 
      ggccaatgg gaagaagca atttcaagt ctggagtac 
      ataatgggc gaacaagct tgccatagg gcaggcttg 
      cacttaatt atctgttgc agagctttg gaagataga 
      gatagaatg aaaacagca ttttccatg ttacttaac 
      gttcctctg gaagtcgaa gatgaagaa aaatacaac 
      cttgaacag gacaatcca gaagatgta ctaatcgct 
      atggctatt caaaacgac gatttacat aaatacgaa 
      ataaaaaaa tgcaaggac gccgaatac tgcatttta 
      acggcagct aagcttata gcaccttta attgaagac 
      acatttagt ttaggctat gactggtgc gtaatgtcg 
      ataaaaaat tcagaatac gctcgactg gcctcaaat 
      ctcgaaatt aataaagcg gtaatgttt ttgaaacaa 
      aaccaattg cctgaagca atcgacacg ttgaaagcc 
      tttgaaaaa gactcagtt atcgcaacc aacgctgca 
      attaatttg agtttcatt tattacttg caaggagac 
      atcgaaaat gcaacgaaa tacgcgcaa gttgtggaa 
      acaaatccc acaaaaagt gcggacggt tatgtaaac 
      ctgggagcg tgtttaatg gcgaaagga cagccggaa 
      gatgccatt aactcgttc aaaaacgcc ttggagtta 
      gatccgact cactttgag gcaatttac aacctcggt 
      ttggtgctg aaacggcaa ggacactac gcggaagcc 
      cttcagtgt tttcagaga ttttcaggc tcgcttgcc 
      cttctccca aatgtggtt taccaagtt gctaattta 
      ttagaattg aagggtgac agcgaagcc gctgccgac 
      atgtaccaa caattgctg ggtcttgta ccaacggac 
      gctggggcg ctgcaaaaa atgggcgag ttgtacgac 
      cacgatggt gataaacaa caagcccac cactaccac 
      attgaatct ttccggtac tacccggca aatttgtcg 
      gtaattaac tggctgggt tcgtactac atcgaaatg 
      caagtggtg gaaagagcc ctcgtctac ttcgaaaaa 
      gcggccctt atgcagccc aacgagccc aaatggaac 
      atgatggtg gccggctgc caccgaaga agtgggaac 
      atgcatcgt gccttaacc ttgtaccaa gaaatccac 
      cggaaattc cccgaaaat gtggaatgt ttgcgcttt 
      ctggtccgg ttatgcaac gacatgggg atgagagag 
      gctcaggat tatattctc gagttgaaa aaaatcgaa 
      aaggcgaag gaagtcagg gaacgggtt aatagcagc 
      cgccccagt tcccgcagg agcaacagt ggactatcg 
      tcgagggcc ggctccgga tttagccca gttccggaa 
      cacggacca ctgacttct gccaaccga aacttgagg 
      agctcgcgc ctgattcgg gcacacaat agcgcggaa 
      agtacagac tccggattt ggacaacca atgatagac 
      gctagttat tcggatcca ttgggtcca cagccactc 
      cggccgagg acaggcgct ggaaaacct ttggatttt 
      gatgatttt gcaaacgat gagttagga gatgatttg 
      ctacctgag taa
    mRNA sequence
    • >TC003554 RA
      tgctagggc aacccccaa catcaacaa acacctacg 
      ccttcagcc ccaacttat cgacgaaac gagtctttc 
      agaaggtta aacctatta gtaaacaat caataaaca 
      aacccagaa accccttca ttacctaat tctttaaat 
      accgcaaaa tataccgaa aaaatgacc gcaatagag 
      tcgcctgat ttcattgca atcaacaac acgtcatcc 
      ccaaccgac gattttgta acaaccaaa agtgtaaga 
      atcgccgaa atggccaga ttatcaacc cctaagccc 
      ataatgtac ctgggatca accacagag tttcaaaga 
      caaggaacc gggattccc aagaccccc tacaagcct 
      ggaacggga tacagggcc gggactggg ttcaaaccc 
      acgactaac tttaagcag acgagtttt gaaaaaccg 
      ggaactggg tataaaccc ggaactggg ttcagacca 
      gggactggg gccgatgtg gtgaacagg cccatgacg 
      gcggtgcgc ggggctggg tacacctcc catgggaaa 
      cctttcgac cctttgaac caagctgct tcggcccca 
      acgccgcct ctggagttg caaaaggat gactctccc 
      gaacagaaa attcggcaa caaaaggca aaaatcatg 
      cagcttgtg gaagaggcg tgtgtcgcg gaatgcgag 
      ggtgactat cggaagtcg ctctctaag gctaaagaa 
      gcgtcaaat aaggagcgg gttttgatc aaaatgcag 
      gaacagtct gatctgagt gacttgcac gatattgat 
      ttaactttt gttgttttg ttcacactt ggtaaccag 
      tacgcagcc aatgaatta tacacggaa gcactcagc 
      acgtatcaa atgatcata aaaaatcga atgttttca 
      aatgcacac cgtttgaag ataaatatg ggcaatatt 
      tatttcaaa caaggccag ttccaaatg gcggtcaaa 
      atgtatcgc atggctttg gaccaagtg tcttcaagt 
      caaaaaaat ctgagaatc aaaataatg cacaatatt 
      gcgttagtt tttatcaaa atgggccaa tgggaagaa 
      gcaatttca agtctggag tacataatg ggcgaacaa 
      gcttgccat agggcaggc ttgcactta attatctgt 
      tgcagagct ttggaagat agagataga atgaaaaca 
      gcattttcc atgttactt aacgttcct ctggaagtc 
      gaagatgaa gaaaaatac aaccttgaa caggacaat 
      ccagaagat gtactaatc gctatggct attcaaaac 
      gacgattta cataaatac gaaataaaa aaatgcaag 
      gacgccgaa tactgcatt ttaacggca gctaagctt 
      atagcacct ttaattgaa gacacattt agtttaggc 
      tatgactgg tgcgtaatg tcgataaaa aattcagaa 
      tacgctcga ctggcctca aatctcgaa attaataaa 
      gcggtaatg tttttgaaa caaaaccaa ttgcctgaa 
      gcaatcgac acgttgaaa gcctttgaa aaagactca 
      gttatcgca accaacgct gcaattaat ttgagtttc 
      atttattac ttgcaagga gacatcgaa aatgcaacg 
      aaatacgcg caagttgtg gaaacaaat cccacaaaa 
      agtgcggac ggttatgta aacctggga gcgtgttta 
      atggcgaaa ggacagccg gaagatgcc attaactcg 
      ttcaaaaac gccttggag ttagatccg actcacttt 
      gaggcaatt tacaacctc ggtttggtg ctgaaacgg 
      caaggacac tacgcggaa gcccttcag tgttttcag 
      agattttca ggctcgctt gcccttctc ccaaatgtg 
      gtttaccaa gttgctaat ttattagaa ttgaagggt 
      gacagcgaa gccgctgcc gacatgtac caacaattg 
      ctgggtctt gtaccaacg gacgctggg gcgctgcaa 
      aaaatgggc gagttgtac gaccacgat ggtgataaa 
      caacaagcc caccactac cacattgaa tctttccgg 
      tactacccg gcaaatttg tcggtaatt aactggctg 
      ggttcgtac tacatcgaa atgcaagtg gtggaaaga 
      gccctcgtc tacttcgaa aaagcggcc cttatgcag 
      cccaacgag cccaaatgg aacatgatg gtggccggc 
      tgccaccga agaagtggg aacatgcat cgtgcctta 
      accttgtac caagaaatc caccggaaa ttccccgaa 
      aatgtggaa tgtttgcgc tttctggtc cggttatgc 
      aacgacatg gggatgaga gaggctcag gattatatt 
      ctcgagttg aaaaaaatc gaaaaggcg aaggaagtc 
      agggaacgg gttaatagc agccgcccc agttcccgc 
      aggagcaac agtggacta tcgtcgagg gccggctcc 
      ggatttagc ccagttccg gaacacgga ccactgact 
      tctgccaac cgaaacttg aggagctcg cgcctgatt 
      cgggcacac aatagcgcg gaaagtaca gactccgga 
      tttggacaa ccaatgata gacgctagt tattcggat 
      ccattgggt ccacagcca ctccggccg aggacaggc 
      gctggaaaa cctttggat tttgatgat tttgcaaac 
      gatgagtta ggagatgat ttgctacct gagtaatta 
      attgtcatg ctatacgca tagtaaaat gtagtaaag 
      gtgtgaaaa gaaactatt ttttcaatt