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Gene information
General information
TC003548 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2489838..2497872
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003548
    iBeetle screen iB_03574
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003548 RA
      MEDGSHVYL DDSGPAEAD NSDICYTSF FSNGSSEHV 
      KQDDETDNI DPKISATLE QLSNKIIRV RDLIKTEQK 
      MRDDNVNEY LKLAANADK QQVQRIKAV FEKKNQKSA 
      QVISQLQKK LDTYQKRVH ELETHGLVQ SHRPPREVL 
      RDMGQGLKN VGGNIRDGI TGFSGSVMS KPREFAHLI 
      KNKFGSADN ISQLSTWYI GPGATGSEG SAANGGTGS 
      DQGEKVHHG SATLPATLH TATSQTSMG QAHRAKFPS 
      DEGSECSSV TSDSIGRFG RGQETGSNQ DSLKALYKQ 
      LTTKQEEIE ALKEKLEQM KSLPQEIQY LHAALQEER 
      FRTERLEEQ INDLTELHQ NEVENLKQA MADMEEKVQ 
      YQSEERLRD IHEILEHCQ TKIQKMEHQ ALQQQQYVT 
      LEGLDNSNA RALVVKLIN ILLTVLQVV LLLVATGAG 
      IIMPFLRTR
    CDS sequence
    • >TC003548 RA
      atggaagac ggaagtcac gtctacttg gacgattcg 
      gggccagca gaagccgat aattccgat atttgttac 
      acttcgttt ttttccaac ggtagcagt gaacacgtc 
      aagcaggac gatgagacc gacaacatc gatcccaaa 
      attagtgca acacttgaa cagttatcg aataaaata 
      attagagtg agagatctc ataaagacg gaacagaaa 
      atgagagac gacaacgta aacgaatat cttaagctg 
      gcagctaac gccgataaa cagcaagtc caaaggatc 
      aaagccgtt ttcgagaaa aagaatcaa aagagtgca 
      caggtcata tcgcagttg caaaaaaag ctggacacg 
      tatcagaaa cgcgttcat gaattagaa acacacgga 
      ttagttcaa agtcatagg ccccccaga gaggtttta 
      cgtgatatg ggacaaggc ttgaagaat gtgggtgga 
      aatatccga gatggtatt acagggttc agcggatcg 
      gtcatgtcg aagcctaga gagtttgct cacctaatt 
      aaaaataaa ttcggaagt gcggataac atcagccag 
      ctttcgacg tggtacata gggcccgga gcaaccgga 
      tctgaaggt agcgcagcc aatggtggt accggaagt 
      gaccaaggg gagaaggtg caccatggg tcggctacc 
      ttgccggcg acgttgcac acggccacg tcgcagacc 
      tcgatgggt caagcccat agggcgaag tttcccagt 
      gatgagggc agcgagtgt agcagcgtt accagcgat 
      agtatcgga agattcggg agaggtcaa gagaccggt 
      tcaaatcaa gactcactc aaagccctg tacaaacaa 
      ctaacaaca aaacaggaa gagatcgaa gctttgaaa 
      gaaaagctg gagcagatg aagtcgcta ccgcaggaa 
      atacagtat ctgcatgcc gcactgcag gaggagaga 
      tttcgaacg gaacgatta gaggagcaa attaacgat 
      ttaaccgaa ctgcatcag aacgaagtc gagaatttg 
      aagcaggca atggccgac atggaagag aaagtgcaa 
      tatcaaagc gaggaacgt ctcagggat atccacgaa 
      attttagaa cactgtcag actaaaata caaaaaatg 
      gagcatcaa gcgctccag caacagcag tacgtcacg 
      ctcgaaggc ttggataat tccaacgcc cgagctcta 
      gttgtcaaa ttaataaac atactgctc actgttctc 
      caggttgtc ttactccta gtggccaca ggggccggt 
      atcataatg ccgtttctc cgtacaagg taa
    mRNA sequence
    • >TC003548 RA
      tgaaaattg tcacgaaaa ttgtatctc gagcacggg 
      ttccgactc tgtgcagtt acgccacac atttacgtg 
      agccgagta cttcgcaaa ggtcgttgt aataactgt 
      aaaaacgct aatctgaca ttttcagtt atgtacgtg 
      ttctaatct ttttaattt gctttattt gcccaattt 
      tcgtgcacg attttttat ttccaatgc catcgttaa 
      acgtttcca attggcgaa aaacatctg gctcccatt 
      agcttcacg aaattctcc gttttagca gaatgaccg 
      tgaaaaaag tacaagacc ggaaactca ccggagacg 
      tactttttc cggacatta acaccgaat gttttcgca 
      aataattgg caagaattt gtttacttg acccgagct 
      agtacaaga gttgagtaa tttttggag taaataatt 
      gtttacatt tgctattag catcgcgat atttgctcg 
      ttgatctct cgagttcta attagttgc gtgctccgt 
      tatcgcaca atgagaaca aaattgagg gtgagtggg 
      tatttagat aaattattt aattacggc gttaccaac 
      cggcactat gcgaatttt caaagactc tgatttatt 
      ttgaattaa aaaaagtgt ttggaatta atttcgtca 
      agatgctcg cgatggata acgattacg accattacg 
      aatggctca tccctaacg aaatattaa tggtacgtg 
      tttgggaag ttgtttttt tcaaaatct ggcgtaaaa 
      tgttgttac aggcgttac acgttgttt tcgttgcag 
      tcccaagat ggtggagta acgcactcc tcgcaccgg 
      ccctcgaga caccgttcc cccacgacc agcaccaac 
      agaacggac atggaagac ggaagtcac gtctacttg 
      gacgattcg gggccagca gaagccgat aattccgat 
      atttgttac acttcgttt ttttccaac ggtagcagt 
      gaacacgtc aagcaggac gatgagacc gacaacatc 
      gatcccaaa attagtgca acacttgaa cagttatcg 
      aataaaata attagagtg agagatctc ataaagacg 
      gaacagaaa atgagagac gacaacgta aacgaatat 
      cttaagctg gcagctaac gccgataaa cagcaagtc 
      caaaggatc aaagccgtt ttcgagaaa aagaatcaa 
      aagagtgca caggtcata tcgcagttg caaaaaaag 
      ctggacacg tatcagaaa cgcgttcat gaattagaa 
      acacacgga ttagttcaa agtcatagg ccccccaga 
      gaggtttta cgtgatatg ggacaaggc ttgaagaat 
      gtgggtgga aatatccga gatggtatt acagggttc 
      agcggatcg gtcatgtcg aagcctaga gagtttgct 
      cacctaatt aaaaataaa ttcggaagt gcggataac 
      atcagccag ctttcgacg tggtacata gggcccgga 
      gcaaccgga tctgaaggt agcgcagcc aatggtggt 
      accggaagt gaccaaggg gagaaggtg caccatggg 
      tcggctacc ttgccggcg acgttgcac acggccacg 
      tcgcagacc tcgatgggt caagcccat agggcgaag 
      tttcccagt gatgagggc agcgagtgt agcagcgtt 
      accagcgat agtatcgga agattcggg agaggtcaa 
      gagaccggt tcaaatcaa gactcactc aaagccctg 
      tacaaacaa ctaacaaca aaacaggaa gagatcgaa 
      gctttgaaa gaaaagctg gagcagatg aagtcgcta 
      ccgcaggaa atacagtat ctgcatgcc gcactgcag 
      gaggagaga tttcgaacg gaacgatta gaggagcaa 
      attaacgat ttaaccgaa ctgcatcag aacgaagtc 
      gagaatttg aagcaggca atggccgac atggaagag 
      aaagtgcaa tatcaaagc gaggaacgt ctcagggat 
      atccacgaa attttagaa cactgtcag actaaaata 
      caaaaaatg gagcatcaa gcgctccag caacagcag 
      tacgtcacg ctcgaaggc ttggataat tccaacgcc 
      cgagctcta gttgtcaaa ttaataaac atactgctc 
      actgttctc caggttgtc ttactccta gtggccaca 
      ggggccggt atcataatg ccgtttctc cgtacaagg 
      taaacaatt gtcgatttg ttgttttct cctg