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Gene information
General information
TC003544 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2440628..2463748
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003544
    iBeetle screen iB_03573
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003544 RA
      MQFFALVVP DLPAERSIS QLEVVGSKT VSLEAESLA 
      GCQVQALVL ANNRLQHVA DRAFSSLWK SLTSLDLSY 
      NQLDSVPFL ALKELRSLQ WINLHGNQI SSIGSEWSH 
      VKNTLTTLF LGENDITEV ADEQPDHSS KVSHGLREF 
      KSLIWVNLD GNRMYKIHK HSLPLTLQT ASISHNLIE 
      NFPLEIVSG LPHLQWLYL RGNHIKTIP EHTFARKVW 
      LEKIDLGEN YLKSLPRSP FNSSVYIRD LNLAFNDFK 
      TLSSQSFAG LQCGRIILS YNMLEDLEI RTFEGIEDT 
      LEYLDFDHN NFQRIPYAL GQLNSLKYL YLSSNLLSE 
      IPERAFDTF CSSLKAVSL SGNRLTRIP VETLQNCSK 
      ISHFNIGFN EIYEIGESD FSWGSNIKS LILGNNRIT 
      SLKSHIFAD LQQLKELSL SFNPLRVID SSAFAGLEG 
      LESLEVSFG LDRDDLPHE IFKPLTNLK WLSVDNNNF 
      DTVPEFSLD SLPELKYLN IESNKIRTI PINLLKPAI 
      HSKLKDIRL SNNEISTVR TDTFKSLNS LETVLLSNN 
      RIRAIEADS FNDLPALNK LILANNLIS KLHSRAFSN 
      LPSLAKLDL QNNFLSEFS FGCFANLSA PLHLNLSRN 
      QIISCNSDL KILNVHVID LRYNNLARI PKCLENTAL 
      LKKLHLDFN IIARLDHNS FMHLTSLEQ LSLQQNNIM 
      SVSRKAFAG LQNLQILDL SKNLVSQLH PSQFANMPQ 
      LRVLDLSSN SLNYLPKDV FQNTVIEML DLSYNSFSV 
      VPSLSLSDV GLSLRHLSI SSNNIEHID STTFPDIPF 
      LHHLNLSNN KLTILPDNV FTSLGLLQV LDLSSNPLR 
      ANFKELFHY AQSLKHLNL ANSGITSTP HLPLPNMVH 
      LNLSHNHIE AISKNSVQE LGKLKSIDL SHNQLFEVP 
      AHLWIHLPR LKSLDLSFN PIKEIVADS FYGLSNLQD 
      LNIQGLRFL DRFESKAIQ QIKILNSLA IQTWPNIDN 
      FNEQFCNLM ANLQQLRIL KIHVMESVL DEQLLCVKN 
      KKIRHLEIT GRNLKMIDR DAFVRFGRN PDLVLRITG 
      TEIEELPPG LFSSMYKVS YLTIDLRNN MLSYLSPEI 
      FYGNTTTWK NVGTTLISG GLTISENPF RCGCHLAWL 
      GHWLRRWTR ESLQSHNAP VETAMRMND MVKEATCTD 
      ITSGTRIPI VQLPPEDMS CHASALSDA PNLNKIPVM 
      FVLIYLMTY NFVLR
    CDS sequence
    • >TC003544 RA
      atgcaattt ttcgctctg gttgttcca gatttgcct 
      gcggaaaga agcattagc caactggaa gtggtggga 
      tcgaagact gtcagcttg gaagctgag agtttggca 
      ggatgccaa gttcaggct ctcgtcctg gccaataac 
      cgcctgcaa catgtggcg gatcgagcg tttagttcg 
      ttatggaaa agcctcact tcgttggat ctgagctat 
      aatcaacta gacagtgtt cctttcctc gcactcaaa 
      gagctgcga agtctgcag tggattaat ttgcacggg 
      aatcaaata tcgtcgatc ggcagcgaa tggtcgcac 
      gtgaagaac actttgacg acgctgttc ctgggcgag 
      aacgacatc accgaagtg gcggatgag cagccggat 
      cacagctct aaagtgtcg cacggtctg cgagagttc 
      aagtcgctc atttgggtg aacttggac ggaaaccga 
      atgtataaa atccataag cactctctg ccgctcact 
      cttcaaacc gccagtatt tcgcataat ttgattgaa 
      aactttcca cttgaaatc gtcagcggc ctgcctcat 
      ttgcaatgg ctgtatttg cgagggaat cacatcaag 
      acgataccc gagcacacg ttcgccagg aaagtatgg 
      ctggagaaa atcgatctt ggcgagaac tatctgaaa 
      agtttgccg agatccccg tttaacagt tctgtttat 
      ataagggat ttaaatctg gcttttaac gacttcaag 
      acgctgtcg agccaaagc tttgccggt ctccagtgc 
      ggaaggata attctttcg tacaatatg ctcgaggat 
      ctcgaaatt aggacgttc gagggcatc gaggacact 
      ttggagtat ctcgatttc gatcacaat aatttccag 
      cggattccc tacgccttg ggccaactc aactcgctt 
      aaatacctt tacttatcg tcgaacttg ctaagtgaa 
      ataccggaa cgagccttc gacactttc tgttccagt 
      ctgaaagcg gtgagcttg agcggaaac cgtttgacg 
      agaattccg gttgaaaca ttgcaaaat tgctcgaaa 
      atttcccac tttaacatc ggttttaat gaaatatac 
      gaaatcggc gagagcgat ttcagctgg ggctcgaac 
      atcaagagc ctcatactg gggaacaat cgaattaca 
      agcttgaag agtcacatc ttcgccgat ttgcagcaa 
      cttaaagaa ctcagtctg agttttaac ccgttgcgg 
      gtcatcgac agcagcgcc ttcgccggc ctcgaaggc 
      ttagagagt ttggaagta tcgttcgga ctcgaccga 
      gacgacctc ccgcacgaa attttcaaa ccgctaaca 
      aacttgaaa tggctttcc gtcgacaac aataacttc 
      gatacggtc ccggaattc tctttagac tcgcttccc 
      gaactcaaa tatctcaat atcgagtcg aacaagatc 
      cggacgatt ccgatcaac ttattaaaa ccggccatc 
      cactccaaa ctcaaagac atccgcttg tccaacaac 
      gaaatatcc acagtccgc accgacacg ttcaagtcg 
      ctaaacagc ctcgagacc gttcttctc tccaataat 
      cgcatcagg gccatcgaa gccgatagt tttaacgac 
      ctcccagca ctcaacaag ctgatcctc gccaataat 
      ctaatatcg aaactccac agtcgcgct ttctccaac 
      ttgccgtcc ttggcgaag ctcgactta cagaacaat 
      tttctatcg gaattctcc ttcggatgc ttcgccaac 
      ttatccgct cccttacat ctcaacctg agtcgcaac 
      caaatcatc tcctgcaac tccgacttg aaaatcctc 
      aacgtgcac gtgatcgat ttgcgctac aacaacctc 
      gcccgaatc cccaagtgt ctcgaaaac accgccctc 
      ctcaaaaag ctccacctg gacttcaac ataatcgcc 
      cgcctcgac cacaacagc ttcatgcac ttgacctcg 
      ctcgagcag ctcagcttg cagcaaaac aacatcatg 
      tccgtcagc aggaaggcg ttcgccggc ctccagaac 
      ctccaaatc ctggacctg tccaagaac ctcgtctcg 
      cagctgcac cccagccag ttcgccaac atgccgcag 
      ctgcgcgtg ctcgacctg agcagcaac agcctcaac 
      tacctcccc aaagacgtg ttccagaac accgtcatc 
      gagatgctg gatctgagc tacaactcc ttcagcgtg 
      gtgcccagt ctgagcctc tccgacgtc gggctgagc 
      ctgcgccac ctctccatc agctcgaac aacatcgag 
      cacatcgac agcaccacc ttcccggac atccccttc 
      ctgcaccac ctcaatctg agcaacaac aagctgacg 
      atcctcccc gacaacgtt ttcaccagc ttgggcttg 
      ctgcaagtg ctcgacttg agttcgaat cctctacgt 
      gccaacttc aaagaactg ttccactac gcgcaaagt 
      ttgaagcat ctaaacttg gccaattcg gggattacg 
      tccactcca catctgccc ctgcccaac atggtccac 
      ctcaactta tcccacaat cacatcgaa gcaatcagc 
      aagaactcg gtgcaagag ttgggcaaa ttgaaatcg 
      atcgatctg agccataat cagttgttc gaagtgccc 
      gcgcatctc tggatacac ttaccgaga ttaaaatcg 
      ctggatttg tcctttaac ccgattaaa gaaattgtc 
      gccgatagt ttttacggg ttatccaat ttgcaagat 
      ttgaatatt caagggctg aggtttctc gatcggttc 
      gaaagcaaa gcgatccag caaatcaag attttgaac 
      tcgctggcg atacagacg tggccaaac attgacaat 
      tttaatgaa cagttttgc aatttaatg gcgaatttg 
      caacagttg aggattttg aagatacat gtcatggaa 
      tcggtgctt gatgagcaa ctcttgtgt gtgaaaaac 
      aagaagatt aggcatttg gaaatcacg gggaggaat 
      ttgaaaatg atagatagg gacgctttc gttagattt 
      gggaggaat cctgattta gttttgagg attacgggg 
      accgaaatt gaggagttg cctccggga cttttctcc 
      agcatgtac aaagtttca tatttaacg atagatttg 
      aggaacaat atgttgtct tatcttagt ccagaaatc 
      ttttatggg aatacaaca acttggaag aacgttggg 
      acgacttta atatcaggt ggcttaacc atctcagag 
      aatcccttc cgctgcggt tgccacttg gcctggctg 
      ggacattgg ttgcggagg tggacaagg gaaagtttg 
      cagtcccac aacgcgcct gtggaaacc gcgatgcgg 
      atgaacgac atggtgaag gaggcgacg tgtacggac 
      atcacctcc gggacgcgg atacccatt gtgcagttg 
      cccccggag gacatgagt tgccatgcg agtgcttta 
      agtgacgct ccgaatctg aataaaatt cctgtgatg 
      tttgttttg atatacctt atgacgtac aatttcgtt 
      ctgcgttag
    mRNA sequence
    • >TC003544 RA
      ggtcacaag tcaacttca ggaattgac caatattta 
      tgcaatttt tcgctctgg ttgttccag atttgcctg 
      cggaaagaa gcattagcc aactggaag tggtgggat 
      cgaagactg tcagcttgg aagctgaga gtttggcag 
      gatgccaag ttcaggctc tcgtcctgg ccaataacc 
      gcctgcaac atgtggcgg atcgagcgt ttagttcgt 
      tatggaaaa gcctcactt cgttggatc tgagctata 
      atcaactag acagtgttc ctttcctcg cactcaaag 
      agctgcgaa gtctgcagt ggattaatt tgcacggga 
      atcaaatat cgtcgatcg gcagcgaat ggtcgcacg 
      tgaagaaca ctttgacga cgctgttcc tgggcgaga 
      acgacatca ccgaagtgg cggatgagc agccggatc 
      acagctcta aagtgtcgc acggtctgc gagagttca 
      agtcgctca tttgggtga acttggacg gaaaccgaa 
      tgtataaaa tccataagc actctctgc cgctcactc 
      ttcaaaccg ccagtattt cgcataatt tgattgaaa 
      actttccac ttgaaatcg tcagcggcc tgcctcatt 
      tgcaatggc tgtatttgc gagggaatc acatcaaga 
      cgatacccg agcacacgt tcgccagga aagtatggc 
      tggagaaaa tcgatcttg gcgagaact atctgaaaa 
      gtttgccga gatccccgt ttaacagtt ctgtttata 
      taagggatt taaatctgg cttttaacg acttcaaga 
      cgctgtcga gccaaagct ttgccggtc tccagtgcg 
      gaaggataa ttctttcgt acaatatgc tcgaggatc 
      tcgaaatta ggacgttcg agggcatcg aggacactt 
      tggagtatc tcgatttcg atcacaata atttccagc 
      ggattccct acgccttgg gccaactca actcgctta 
      aataccttt acttatcgt cgaacttgc taagtgaaa 
      taccggaac gagccttcg acactttct gttccagtc 
      tgaaagcgg tgagcttga gcggaaacc gtttgacga 
      gaattccgg ttgaaacat tgcaaaatt gctcgaaaa 
      tttcccact ttaacatcg gttttaatg aaatatacg 
      aaatcggcg agagcgatt tcagctggg gctcgaaca 
      tcaagagcc tcatactgg ggaacaatc gaattacaa 
      gcttgaaga gtcacatct tcgccgatt tgcagcaac 
      ttaaagaac tcagtctga gttttaacc cgttgcggg 
      tcatcgaca gcagcgcct tcgccggcc tcgaaggct 
      tagagagtt tggaagtat cgttcggac tcgaccgag 
      acgacctcc cgcacgaaa ttttcaaac cgctaacaa 
      acttgaaat ggctttccg tcgacaaca ataacttcg 
      atacggtcc cggaattct ctttagact cgcttcccg 
      aactcaaat atctcaata tcgagtcga acaagatcc 
      ggacgattc cgatcaact tattaaaac cggccatcc 
      actccaaac tcaaagaca tccgcttgt ccaacaacg 
      aaatatcca cagtccgca ccgacacgt tcaagtcgc 
      taaacagcc tcgagaccg ttcttctct ccaataatc 
      gcatcaggg ccatcgaag ccgatagtt ttaacgacc 
      tcccagcac tcaacaagc tgatcctcg ccaataatc 
      taatatcga aactccaca gtcgcgctt tctccaact 
      tgccgtcct tggcgaagc tcgacttac agaacaatt 
      ttctatcgg aattctcct tcggatgct tcgccaact 
      tatccgctc ccttacatc tcaacctga gtcgcaacc 
      aaatcatct cctgcaact ccgacttga aaatcctca 
      acgtgcacg tgatcgatt tgcgctaca acaacctcg 
      cccgaatcc ccaagtgtc tcgaaaaca ccgccctcc 
      tcaaaaagc tccacctgg acttcaaca taatcgccc 
      gcctcgacc acaacagct tcatgcact tgacctcgc 
      tcgagcagc tcagcttgc agcaaaaca acatcatgt 
      ccgtcagca ggaaggcgt tcgccggcc tccagaacc 
      tccaaatcc tggacctgt ccaagaacc tcgtctcgc 
      agctgcacc ccagccagt tcgccaaca tgccgcagc 
      tgcgcgtgc tcgacctga gcagcaaca gcctcaact 
      acctcccca aagacgtgt tccagaaca ccgtcatcg 
      agatgctgg atctgagct acaactcct tcagcgtgg 
      tgcccagtc tgagcctct ccgacgtcg ggctgagcc 
      tgcgccacc tctccatca gctcgaaca acatcgagc 
      acatcgaca gcaccacct tcccggaca tccccttcc 
      tgcaccacc tcaatctga gcaacaaca agctgacga 
      tcctccccg acaacgttt tcaccagct tgggcttgc 
      tgcaagtgc tcgacttga gttcgaatc ctctacgtg 
      ccaacttca aagaactgt tccactacg cgcaaagtt 
      tgaagcatc taaacttgg ccaattcgg ggattacgt 
      ccactccac atctgcccc tgcccaaca tggtccacc 
      tcaacttat cccacaatc acatcgaag caatcagca 
      agaactcgg tgcaagagt tgggcaaat tgaaatcga 
      tcgatctga gccataatc agttgttcg aagtgcccg 
      cgcatctct ggatacact taccgagat taaaatcgc 
      tggatttgt cctttaacc cgattaaag aaattgtcg 
      ccgatagtt tttacgggt tatccaatt tgcaagatt 
      tgaatattc aagggctga ggtttctcg atcggttcg 
      aaagcaaag cgatccagc aaatcaaga ttttgaact 
      cgctggcga tacagacgt ggccaaaca ttgacaatt 
      ttaatgaac agttttgca atttaatgg cgaatttgc 
      aacagttga ggattttga agatacatg tcatggaat 
      cggtgcttg atgagcaac tcttgtgtg tgaaaaaca 
      agaagatta ggcatttgg aaatcacgg ggaggaatt 
      tgaaaatga tagataggg acgctttcg ttagatttg 
      ggaggaatc ctgatttag ttttgagga ttacgggga 
      ccgaaattg aggagttgc ctccgggac ttttctcca 
      gcatgtaca aagtttcat atttaacga tagatttga 
      ggaacaata tgttgtctt atcttagtc cagaaatct 
      tttatggga atacaacaa cttggaaga acgttggga 
      cgactttaa tatcaggtg gcttaacca tctcagaga 
      atcccttcc gctgcggtt gccacttgg cctggctgg 
      gacattggt tgcggaggt ggacaaggg aaagtttgc 
      agtcccaca acgcgcctg tggaaaccg cgatgcgga 
      tgaacgaca tggtgaagg aggcgacgt gtacggaca 
      tcacctccg ggacgcgga tacccattg tgcagttgc 
      ccccggagg acatgagtt gccatgcga gtgctttaa 
      gtgacgctc cgaatctga ataaaattc ctgtgatgt 
      ttgttttga tatacctta tgacgtaca atttcgttc 
      tgcgttagt gaacctaac agtatttgt acataacgt 
      ctagtgtga gactacttc ttccattta ttttactaa 
      aatttttaa tacatcaat tgttatttt ttgataatg 
      taaatcgtg tacataata aaattaaaa catatcatg 
      ctcgtatag aaggataga acttattta caatgattg 
      ctcaaaatg ggagatgtc aatcaaaaa tgtgcacac 
      gacttgttt cttattaac gtttagcta attatagtg 
      ttttgtaat gatcaataa agtatataa attgtt