| General information | |||
| TC003537 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6610545..6625294 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003537 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03571 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
- >TC003537 RA
MNSAEELSK YKENIDSYY SPTLSRRLL GYGYDFPEK SPKKEKKWS LGSLFRRKK KDESDSSSD DDLQKKGFL GRKKRKPDK KKKPNKPIG TFDHVVLSR NSHLYSNGF NGYDEAGIF SDPGNGFGY GSKSLPKTQ SQEIVKQPV KNATVEEVS NSRSTSISG STEVGRKSR KGLAKVRAE ARRVNLKRD SSSDEDSQR SVSSTRFRS DESLGQGYR DGSLSRRSR AARTERYLK RHSRDGENP KDFLRLSKS DAENSVPQW KTSDDSNRS LSRSPLLPP SLNKTKTIY STLSQSPNI SGLSTIPPS HSNSRIKVS NSTSNPSYK PPLSMNDYN YSTRKLSND YLSNQRSLS CDANIHKSL PVEAESEII HVQFPIGKP NNRLRNLSL IESRHVNAC HARQPPPPP PRDPRRLVT AQYYDNVRP ITCYLDSPM KSKSFHTNS QTPAVQTKK FTSFNKNCR STSEDHIPQ NNVHLIPRP SSATPDASQ RRTQVNTNL ENYNYLTDK KPRSRKPIF IQSNNSLDS VSTDKSETQ KALDFWKQK DKDSRSVKN NVKTKRAKS PQMFTCQTH VQTQVFLPS VLQNDITSE NITKSMDTE KDDSGTNRY MPVLCQEPL AKEEENEDL KRKSSNLEE ALDELEAIY NSLRLGDED LLERAEKRE KDAAEQKLK EEGREVYSN WSTSRGALS DSSFSYEPF DSIGSPRRK RFVRKSQTI DRRADDMAF RKLNKERSA TINDPQGVL SKISYLNAS PVYGKEPKE HEVPNKNSK EPDITLDDV VYRNVKFAN NCPKVVEPQ PPFGIPLGP ITPAANSDY LHAVPDNIP RPMFKPRKI PDLVKDDLA FRNLRKDQG KEPALPPLS PDDLKNNNA SEPKLDLDS LKKKRAVRS LSANIGSII NKNFLSKGL EKHGGESNE SKTLTDIAD AMEIARQVL MEKENKISA TRRAFLSDT DTNNGNDSI TESRMNFLN SLKTPSDHY LQAKPPRGV TTPECKSPR SPKESTPIP VEESRDSSL DDLLTALAT EARETNERI AKELKSFED RKRKKSPAD ENLNNYELT KTLSDIDAV SEQAKLCEK LLEGVVDST DLMASAKPV LEEVGQIDT EATREESVA NVVLTRDDS KNKEKSPVK LSCESDHDY ENLVSDQEL NLDEAPVED VKCTSPFEE HKAELIATF QELKNNVSL DLDGRKEND TNDKENDET VAGEACVGV VDAASNVTE CACFEPFVS NPQTHDSNH DTLSTNHVC LSGVDSSNC PGSSAGLEK CGHLDSLDS LKSPKLVLE TSKLSEHEG TNQILQNQS MICRESDSN KPGPSCDSV MDFRRRSSE RGESGHILD DDGKPSPAW YRDPATVAV ACSYGIACA HQLASLDFV AILGLLFAV LSFIAALIF
- >TC003537 RB
MPLSAVCVS TLSSRPRFW SSHSNIRAK DVQQLTTMN SAEELSKYK ENIDSYYSP TLSRRLLGY GYDFPEKSP KKEKKWSLG SLFRRKKKD ESDSSSDDD LQKKGFLGR KKRKPDKKK KPNKPIGTF DHVVLSRNS HLYSNGFNG YDEAGIFSD PGNGFGYGS KSLPKTQSQ EIVKQPVKN ATVEEVSNS RSTSISGST EVGRKSRKG LAKVRAEAR RVNLKRDSS SDEDSQRSV SSTRFRSDE SLGQGYRDG SLSRRSRAA RTERYLKRH SRDGENPKD FLRLSKSDA ENSVPQWKT SDDSNRSLS RSPLLPPSL NKTKTIYST LSQSPNISG LSTIPPSHS NSRIKVSNS TSNPSYKPP LSMNDYNYS TRKLSNDYL SNQRSLSCD ANIHKSLPV EAESEIIHV QFPIGKPNN RLRNLSLIE SRHVNACHA RQPPPPPPR DPRRLVTAQ YYDNVRPIT CYLDSPMKS KSFHTNSQT PAVQTKKFT SFNKNCRST SEDHIPQNN VHLIPRPSS ATPDASQRR TQVNTNLEN YNYLTDKKP RSRKPIFIQ SNNSLDSVS TDKSETQKA LDFWKQKDK DSRSVKNNV KTKRAKSPQ MFTCQTHVQ TQVFLPSVL QNDITSENI TKSMDTEKD DSGTNRYMP VLCQEPLAK EEENEDLKR KSSNLEEAL DELEAIYNS LRLGDEDLL ERAEKREKD AAEQKLKEE GREVYSNWS TSRGALSDS SFSYEPFDS IGSPRRKRF VRKSQTIDR RADDMAFRK LNKERSATI NDPQGVLSK ISYLNASPV YGKEPKEHE VPNKNSKEP DITLDDVVY RNVKFANNC PKVVEPQPP FGIPLGPIT PAANSDYLH AVPDNIPRP MFKPRKIPD LVKDDLAFR NLRKDQGKE PALPPLSPD DLKNNNASE PKLDLDSLK KKRAVRSLS ANIGSIINK NFLSKGLEK HGGESNESK TLTDIADAM EIARQVLME KENKISATR RAFLSDTDT NNGNDSITE SRMNFLNSL KTPSDHYLQ AKPPRGVTT PECKSPRSP KESTPIPVE ESRDSSLDD LLTALATEA RETNERIAK ELKSFEDRK RKKSPADEN LNNYELTKT LSDIDAVSE QAKLCEKLL EGVVDSTDL MASAKPVLE EVGQIDTEA TREESVANV VLTRDDSKN KEKSPVKLS CESDHDYEN LVSDQELNL DEAPVEDVK CTSPFEEHK AELIATFQE LKNNVSLDL DGRKENDTN DKENDETVA GEACVGVVD AASNVTECA CFEPFVSNP QTHDSNHDT LSTNHVCLS GVDSSNCPG SSAGLEKCG HLDSLDSLK SPKLVLETS KLSEHEGTN QILQNQSMI CRESDSNKP GPSCDSVMD FRRRSSERG ESGHILDDD GKPSPAWYR DPATVAVAC SYGIACAHQ LASLDFVAI LGLLFAVLS FIAALIF
- >TC003537 RA
atgaacagt gctgaagaa ctatcaaaa tataaggag aacatcgat agttactat tctccaact ttatccaga cgcttgtta ggatacgga tatgatttt cccgagaaa tcacccaag aaggagaaa aaatggtct ttggggagt ttatttcga aggaaaaag aaagacgaa agtgacagt agcagtgac gacgacctg cagaagaag ggtttttta ggtcgaaag aaacgaaaa cctgacaag aaaaaaaag ccaaataaa cctatcgga accttcgac catgtcgta ctttcccgt aattcacac ctgtactca aatgggttt aatggctac gatgaagcc ggcattttt tctgacccg ggtaatggt tttggatac ggcagtaaa tcgttacca aagactcaa tcccaggaa attgtaaaa cagcctgtt aaaaatgca acagttgag gaggtcagc aactctagg agcacttcg atctcagga tcaacggaa gttggaaga aaaagccgg aaagggttg gcaaaagta agggcggag gccagaagg gttaatttg aagcgggac agcagttcg gatgaggat tctcaaaga tctgtctct tctactagg tttaggagt gacgaaagt ttgggtcaa gggtaccga gatggctct ttaagccgc cgctcaagg gcggcaagg acagaacgc tacttaaaa cgacattct cgagatggc gagaatcca aaagacttt ttaagactg tcaaaatcg gacgccgaa aattccgta ccacagtgg aaaacgtcc gatgatagt aaccgatcg ttaagtaga agcccttta ctaccacct tcattaaac aaaaccaaa acgatatac tccacctta tcgcagtca cctaacatt tcgggatta tcaactatc cctcctagt catagcaat agtaggatt aaagttagc aattcaacg agtaacccg tcgtacaag ccacctttg tcgatgaac gactataac tacagtact cgaaaacta tcaaatgat tatctttct aatcaacgt agtttatct tgtgatgcc aacatacat aaatccctg cctgtggaa gcggaatcg gaaataatt catgtccag tttcctata ggaaaaccc aataacagg ttacggaat ctgagttta attgagtct cggcatgta aatgcctgt catgcaaga caaccacca cctccacct ccgcgtgat cctagaaga ttagtcacg gctcaatat tacgacaat gtgaggccc atcacgtgc tatttagac agccctatg aaatcgaaa tcgttccac acgaattcg caaactccc gcagtgcaa accaaaaaa ttcacttcg tttaataaa aactgcaga tcgacttcg gaagatcac ataccacaa aacaacgtc catttgata ccacgacca tcgtcggca actcccgac gcttctcaa aggcgcaca caagttaac acaaatctg gaaaattat aattattta actgataag aaaccgcgg agcagaaaa ccgatattt atccagtca aataatagc ttggatagt gtttcaacc gacaagagt gaaactcag aaagcgtta gatttctgg aaacaaaag gacaaggat tccagaagt gttaaaaat aatgtgaaa acgaagaga gcgaaaagt ccacaaatg ttcacttgc cagacgcat gttcaaacc caggtgttc ctacctagc gttcttcag aatgatatt acgtctgag aatataacc aaatcgatg gatactgaa aaagatgat tcggggaca aaccgttac atgcctgtt ctatgccag gagccgttg gctaaagaa gaggaaaat gaagatttg aaacgcaag tcttcaaat ctggaagag gctcttgac gagttggag gcaatttac aacagcttg cgactgggc gatgaggac cttctggaa agagcggag aagcgggaa aaggatgcc gctgagcag aagttaaag gaggaagga cgcgaagtt tattccaac tggagcacg tcgaggggc gctttgagc gactctagt tttagttac gaacctttc gattctata ggttcccct agacgtaag cgttttgtg cgaaaaagc caaactatc gataggaga gccgacgat atggctttc cgaaagttg aataaagaa cgttcagct acaatcaat gatccgcaa ggtgtttta tccaaaatt agttaccta aacgcgtct cctgtttac ggaaaagaa ccaaaggaa catgaagtg ccaaataaa aactctaag gaacctgat attacttta gatgacgtg gtttataga aacgtcaag tttgccaac aattgccca aaggttgtg gaacctcaa ccaccgttc gggataccc ttagggccc ataacaccg gctgcgaac agtgattat ctccacgct gttcccgat aatattccg cgaccaatg tttaaacca cggaaaatc cctgatttg gttaaggat gatttggct ttccgtaac ttgaggaaa gaccagggc aaggaacct gcccttccg cccttatct ccggacgat cttaagaac aataacgcg tccgaaccg aagctagat ctcgactcg ttgaagaaa aaacgagcc gtgaggtcg ctctccgcc aacattggc agcataatc aataaaaat ttcttatcc aaaggatta gaaaaacac ggtggtgaa agtaacgag tcaaaaaca ttaacagac attgcggac gcgatggaa atcgcacgc caagtcctt atggagaaa gaaaacaaa attagtgcc accagaaga gcatttctt agcgacacc gacacgaac aacggtaac gacagcata accgaaagc cggatgaac tttttgaac agtttgaag actccctcg gaccactac ttacaagca aaaccaccg agaggtgtc accacaccc gaatgtaaa tcaccgaga tcgccgaaa gaatcaact ccgattccg gtcgaagaa tcgagagat tcgtctctt gacgatttg ctcacagct ttggccacc gaagctaga gaaacgaat gaacgcata gccaaagaa ctgaagagt tttgaagac cgcaaacgg aagaaaagc cctgctgat gaaaatttg aacaactac gagctgacg aaaactctg agtgatatt gatgcggtg tcggaacag gcgaaattg tgcgagaaa ttgttggag ggggttgtg gacagtacg gatttgatg gcgtctgca aagccagtt ttggaagag gtggggcag attgatacc gaagctact cgggaagaa tctgttgca aatgttgtt ttaacaaga gacgattcc aaaaacaag gagaaatcg ccagtgaag ctgtcgtgc gagtcagat catgactac gagaacttg gtgtctgat caagagttg aatcttgac gaagctccg gttgaagat gtgaagtgc acgtcgccg ttcgaagaa cacaaggcg gaattgatt gcgactttc caagagttg aagaacaat gttagtctt gatctcgac ggcagaaag gaaaatgat acgaatgat aaagaaaac gatgagacg gttgcaggt gaggcgtgt gtgggtgtt gtagatgcc gcttccaac gtcacagaa tgtgcctgt ttcgaacca tttgtgtct aacccacaa actcacgat tctaaccat gataccctc tctactaat cacgtgtgc ttatcaggc gtcgatagt tcaaattgc ccaggtagc tccgcaggt ttagagaaa tgcggtcat ttagactcc ttagattcg ttgaagtcg cccaaattg gttctagag acgtcgaag ttgtcggaa cacgaagga actaatcaa atattacaa aatcaatcc atgatttgt cgtgaatct gattcgaat aaaccgggg ccctcttgt gatagcgta atggatttt agaaggagg agtagcgag aggggtgaa agtgggcac attctcgat gatgacggg aaaccatct cctgcctgg taccgcgac cccgcaaca gtggctgtt gcatgttct tacggaatt gcatgcgcc catcagctg gcttctctt gattttgtg gctatttta ggacttttg tttgcagtg ttatctttt atagctgct ctaatattt taa
- >TC003537 RB
atgccgctg agtgcagtt tgtgttagt actttgagc agccgacct cgattctgg tcgtctcat tcaaatatt cgtgctaaa gatgtgcaa caactcact actatgaac agtgctgaa gaactatca aaatataag gagaacatc gatagttac tattctcca actttatcc agacgcttg ttaggatac ggatatgat tttcccgag aaatcaccc aagaaggag aaaaaatgg tctttgggg agtttattt cgaaggaaa aagaaagac gaaagtgac agtagcagt gacgacgac ctgcagaag aagggtttt ttaggtcga aagaaacga aaacctgac aagaaaaaa aagccaaat aaacctatc ggaaccttc gaccatgtc gtactttcc cgtaattca cacctgtac tcaaatggg tttaatggc tacgatgaa gccggcatt ttttctgac ccgggtaat ggttttgga tacggcagt aaatcgtta ccaaagact caatcccag gaaattgta aaacagcct gttaaaaat gcaacagtt gaggaggtc agcaactct aggagcact tcgatctca ggatcaacg gaagttgga agaaaaagc cggaaaggg ttggcaaaa gtaagggcg gaggccaga agggttaat ttgaagcgg gacagcagt tcggatgag gattctcaa agatctgtc tcttctact aggtttagg agtgacgaa agtttgggt caagggtac cgagatggc tctttaagc cgccgctca agggcggca aggacagaa cgctactta aaacgacat tctcgagat ggcgagaat ccaaaagac tttttaaga ctgtcaaaa tcggacgcc gaaaattcc gtaccacag tggaaaacg tccgatgat agtaaccga tcgttaagt agaagccct ttactacca ccttcatta aacaaaacc aaaacgata tactccacc ttatcgcag tcacctaac atttcggga ttatcaact atccctcct agtcatagc aatagtagg attaaagtt agcaattca acgagtaac ccgtcgtac aagccacct ttgtcgatg aacgactat aactacagt actcgaaaa ctatcaaat gattatctt tctaatcaa cgtagttta tcttgtgat gccaacata cataaatcc ctgcctgtg gaagcggaa tcggaaata attcatgtc cagtttcct ataggaaaa cccaataac aggttacgg aatctgagt ttaattgag tctcggcat gtaaatgcc tgtcatgca agacaacca ccacctcca cctccgcgt gatcctaga agattagtc acggctcaa tattacgac aatgtgagg cccatcacg tgctattta gacagccct atgaaatcg aaatcgttc cacacgaat tcgcaaact cccgcagtg caaaccaaa aaattcact tcgtttaat aaaaactgc agatcgact tcggaagat cacatacca caaaacaac gtccatttg ataccacga ccatcgtcg gcaactccc gacgcttct caaaggcgc acacaagtt aacacaaat ctggaaaat tataattat ttaactgat aagaaaccg cggagcaga aaaccgata tttatccag tcaaataat agcttggat agtgtttca accgacaag agtgaaact cagaaagcg ttagatttc tggaaacaa aaggacaag gattccaga agtgttaaa aataatgtg aaaacgaag agagcgaaa agtccacaa atgttcact tgccagacg catgttcaa acccaggtg ttcctacct agcgttctt cagaatgat attacgtct gagaatata accaaatcg atggatact gaaaaagat gattcgggg acaaaccgt tacatgcct gttctatgc caggagccg ttggctaaa gaagaggaa aatgaagat ttgaaacgc aagtcttca aatctggaa gaggctctt gacgagttg gaggcaatt tacaacagc ttgcgactg ggcgatgag gaccttctg gaaagagcg gagaagcgg gaaaaggat gccgctgag cagaagtta aaggaggaa ggacgcgaa gtttattcc aactggagc acgtcgagg ggcgctttg agcgactct agttttagt tacgaacct ttcgattct ataggttcc cctagacgt aagcgtttt gtgcgaaaa agccaaact atcgatagg agagccgac gatatggct ttccgaaag ttgaataaa gaacgttca gctacaatc aatgatccg caaggtgtt ttatccaaa attagttac ctaaacgcg tctcctgtt tacggaaaa gaaccaaag gaacatgaa gtgccaaat aaaaactct aaggaacct gatattact ttagatgac gtggtttat agaaacgtc aagtttgcc aacaattgc ccaaaggtt gtggaacct caaccaccg ttcgggata cccttaggg cccataaca ccggctgcg aacagtgat tatctccac gctgttccc gataatatt ccgcgacca atgtttaaa ccacggaaa atccctgat ttggttaag gatgatttg gctttccgt aacttgagg aaagaccag ggcaaggaa cctgccctt ccgccctta tctccggac gatcttaag aacaataac gcgtccgaa ccgaagcta gatctcgac tcgttgaag aaaaaacga gccgtgagg tcgctctcc gccaacatt ggcagcata atcaataaa aatttctta tccaaagga ttagaaaaa cacggtggt gaaagtaac gagtcaaaa acattaaca gacattgcg gacgcgatg gaaatcgca cgccaagtc cttatggag aaagaaaac aaaattagt gccaccaga agagcattt cttagcgac accgacacg aacaacggt aacgacagc ataaccgaa agccggatg aactttttg aacagtttg aagactccc tcggaccac tacttacaa gcaaaacca ccgagaggt gtcaccaca cccgaatgt aaatcaccg agatcgccg aaagaatca actccgatt ccggtcgaa gaatcgaga gattcgtct cttgacgat ttgctcaca gctttggcc accgaagct agagaaacg aatgaacgc atagccaaa gaactgaag agttttgaa gaccgcaaa cggaagaaa agccctgct gatgaaaat ttgaacaac tacgagctg acgaaaact ctgagtgat attgatgcg gtgtcggaa caggcgaaa ttgtgcgag aaattgttg gagggggtt gtggacagt acggatttg atggcgtct gcaaagcca gttttggaa gaggtgggg cagattgat accgaagct actcgggaa gaatctgtt gcaaatgtt gttttaaca agagacgat tccaaaaac aaggagaaa tcgccagtg aagctgtcg tgcgagtca gatcatgac tacgagaac ttggtgtct gatcaagag ttgaatctt gacgaagct ccggttgaa gatgtgaag tgcacgtcg ccgttcgaa gaacacaag gcggaattg attgcgact ttccaagag ttgaagaac aatgttagt cttgatctc gacggcaga aaggaaaat gatacgaat gataaagaa aacgatgag acggttgca ggtgaggcg tgtgtgggt gttgtagat gccgcttcc aacgtcaca gaatgtgcc tgtttcgaa ccatttgtg tctaaccca caaactcac gattctaac catgatacc ctctctact aatcacgtg tgcttatca ggcgtcgat agttcaaat tgcccaggt agctccgca ggtttagag aaatgcggt catttagac tccttagat tcgttgaag tcgcccaaa ttggttcta gagacgtcg aagttgtcg gaacacgaa ggaactaat caaatatta caaaatcaa tccatgatt tgtcgtgaa tctgattcg aataaaccg gggccctct tgtgatagc gtaatggat tttagaagg aggagtagc gagaggggt gaaagtggg cacattctc gatgatgac gggaaacca tctcctgcc tggtaccgc gaccccgca acagtggct gttgcatgt tcttacgga attgcatgc gcccatcag ctggcttct cttgatttt gtggctatt ttaggactt ttgtttgca gtgttatct tttatagct gctctaata ttttaa
- >TC003537 RA
gtggaagtc gaacagtcc acctccctc gctgaaata tcctgacat atgcgcgtc gtcgtcggt gttcctgtt ccacaccag ctgcgcaca gtaattccc tgtcagtag cctcaaact gttttaaag tgcatttaa ctcgaagtt attcagttt aagattttt ttatcgcct caagctgtt tgaaacgta atgattttt gaacttaca tgttgtaat atttcgtca ttttttggt gttgtgttg cctttaaat tttccgttc ctttcttat cagactaat aagtgagtt tcggttgcg acctgtaac attacggat taaaactgc tttcattat caaactttg agcagccga cctcgattc tggtcgtct cattcaaat attcgtgct aaagatgtg caacaactc actactatg aacagtgct gaagaacta tcaaaatat aaggagaac atcgatagt tactattct ccaacttta tccagacgc ttgttagga tacggatat gattttccc gagaaatca cccaagaag gagaaaaaa tggtctttg gggagttta tttcgaagg aaaaagaaa gacgaaagt gacagtagc agtgacgac gacctgcag aagaagggt tttttaggt cgaaagaaa cgaaaacct gacaagaaa aaaaagcca aataaacct atcggaacc ttcgaccat gtcgtactt tcccgtaat tcacacctg tactcaaat gggtttaat ggctacgat gaagccggc attttttct gacccgggt aatggtttt ggatacggc agtaaatcg ttaccaaag actcaatcc caggaaatt gtaaaacag cctgttaaa aatgcaaca gttgaggag gtcagcaac tctaggagc acttcgatc tcaggatca acggaagtt ggaagaaaa agccggaaa gggttggca aaagtaagg gcggaggcc agaagggtt aatttgaag cgggacagc agttcggat gaggattct caaagatct gtctcttct actaggttt aggagtgac gaaagtttg ggtcaaggg taccgagat ggctcttta agccgccgc tcaagggcg gcaaggaca gaacgctac ttaaaacga cattctcga gatggcgag aatccaaaa gacttttta agactgtca aaatcggac gccgaaaat tccgtacca cagtggaaa acgtccgat gatagtaac cgatcgtta agtagaagc cctttacta ccaccttca ttaaacaaa accaaaacg atatactcc accttatcg cagtcacct aacatttcg ggattatca actatccct cctagtcat agcaatagt aggattaaa gttagcaat tcaacgagt aacccgtcg tacaagcca cctttgtcg atgaacgac tataactac agtactcga aaactatca aatgattat ctttctaat caacgtagt ttatcttgt gatgccaac atacataaa tccctgcct gtggaagcg gaatcggaa ataattcat gtccagttt cctatagga aaacccaat aacaggtta cggaatctg agtttaatt gagtctcgg catgtaaat gcctgtcat gcaagacaa ccaccacct ccacctccg cgtgatcct agaagatta gtcacggct caatattac gacaatgtg aggcccatc acgtgctat ttagacagc cctatgaaa tcgaaatcg ttccacacg aattcgcaa actcccgca gtgcaaacc aaaaaattc acttcgttt aataaaaac tgcagatcg acttcggaa gatcacata ccacaaaac aacgtccat ttgatacca cgaccatcg tcggcaact cccgacgct tctcaaagg cgcacacaa gttaacaca aatctggaa aattataat tatttaact gataagaaa ccgcggagc agaaaaccg atatttatc cagtcaaat aatagcttg gatagtgtt tcaaccgac aagagtgaa actcagaaa gcgttagat ttctggaaa caaaaggac aaggattcc agaagtgtt aaaaataat gtgaaaacg aagagagcg aaaagtcca caaatgttc acttgccag acgcatgtt caaacccag gtgttccta cctagcgtt cttcagaat gatattacg tctgagaat ataaccaaa tcgatggat actgaaaaa gatgattcg gggacaaac cgttacatg cctgttcta tgccaggag ccgttggct aaagaagag gaaaatgaa gatttgaaa cgcaagtct tcaaatctg gaagaggct cttgacgag ttggaggca atttacaac agcttgcga ctgggcgat gaggacctt ctggaaaga gcggagaag cgggaaaag gatgccgct gagcagaag ttaaaggag gaaggacgc gaagtttat tccaactgg agcacgtcg aggggcgct ttgagcgac tctagtttt agttacgaa cctttcgat tctataggt tcccctaga cgtaagcgt tttgtgcga aaaagccaa actatcgat aggagagcc gacgatatg gctttccga aagttgaat aaagaacgt tcagctaca atcaatgat ccgcaaggt gttttatcc aaaattagt tacctaaac gcgtctcct gtttacgga aaagaacca aaggaacat gaagtgcca aataaaaac tctaaggaa cctgatatt actttagat gacgtggtt tatagaaac gtcaagttt gccaacaat tgcccaaag gttgtggaa cctcaacca ccgttcggg ataccctta gggcccata acaccggct gcgaacagt gattatctc cacgctgtt cccgataat attccgcga ccaatgttt aaaccacgg aaaatccct gatttggtt aaggatgat ttggctttc cgtaacttg aggaaagac cagggcaag gaacctgcc cttccgccc ttatctccg gacgatctt aagaacaat aacgcgtcc gaaccgaag ctagatctc gactcgttg aagaaaaaa cgagccgtg aggtcgctc tccgccaac attggcagc ataatcaat aaaaatttc ttatccaaa ggattagaa aaacacggt ggtgaaagt aacgagtca aaaacatta acagacatt gcggacgcg atggaaatc gcacgccaa gtccttatg gagaaagaa aacaaaatt agtgccacc agaagagca tttcttagc gacaccgac acgaacaac ggtaacgac agcataacc gaaagccgg atgaacttt ttgaacagt ttgaagact ccctcggac cactactta caagcaaaa ccaccgaga ggtgtcacc acacccgaa tgtaaatca ccgagatcg ccgaaagaa tcaactccg attccggtc gaagaatcg agagattcg tctcttgac gatttgctc acagctttg gccaccgaa gctagagaa acgaatgaa cgcatagcc aaagaactg aagagtttt gaagaccgc aaacggaag aaaagccct gctgatgaa aatttgaac aactacgag ctgacgaaa actctgagt gatattgat gcggtgtcg gaacaggcg aaattgtgc gagaaattg ttggagggg gttgtggac agtacggat ttgatggcg tctgcaaag ccagttttg gaagaggtg gggcagatt gataccgaa gctactcgg gaagaatct gttgcaaat gttgtttta acaagagac gattccaaa aacaaggag aaatcgcca gtgaagctg tcgtgcgag tcagatcat gactacgag aacttggtg tctgatcaa gagttgaat cttgacgaa gctccggtt gaagatgtg aagtgcacg tcgccgttc gaagaacac aaggcggaa ttgattgcg actttccaa gagttgaag aacaatgtt agtcttgat ctcgacggc agaaaggaa aatgatacg aatgataaa gaaaacgat gagacggtt gcaggtgag gcgtgtgtg ggtgttgta gatgccgct tccaacgtc acagaatgt gcctgtttc gaaccattt gtgtctaac ccacaaact cacgattct aaccatgat accctctct actaatcac gtgtgctta tcaggcgtc gatagttca aattgccca ggtagctcc gcaggttta gagaaatgc ggtcattta gactcctta gattcgttg aagtcgccc aaattggtt ctagagacg tcgaagttg tcggaacac gaaggaact aatcaaata ttacaaaat caatccatg atttgtcgt gaatctgat tcgaataaa ccggggccc tcttgtgat agcgtaatg gattttaga aggaggagt agcgagagg ggtgaaagt gggcacatt ctcgatgat gacgggaaa ccatctcct gcctggtac cgcgacccc gcaacagtg gctgttgca tgttcttac ggaattgca tgcgcccat cagctggct tctcttgat tttgtggct attttagga cttttgttt gcagtgtta tcttttata gctgctcta atattttaa aaaagttac aaagcatgt tttggttta attcatgtt tccattttc ttgtcattt atttacccc tagtgaaga ctacttagt tgtacaagt ttgtttaag aaatacgat ccaatgcca aatatatta tgttaagtt ctctgtaga ttttgttgt acaaaattg ttttataaa taaaacgtt atagtttct tttaagttt tattttatc ggaacgaac aatgaaagt tgacgaaag ttaaagaac aggattagc aggttcatt attaatttg aatgtgaaa agcatcgac tttcaaatt attattaat tcacgaaag attggaacg agcgtttac gaatgtttt ttagttctt ttattactt tttacgcaa aataattat agttagttt agacaacca tcaatacac atgcgcttt tgttttttt atgatcggt agattatcc attacttca cctggaatg cgttgtttt attaatcta ttagtgttc ttctgttgc ccacttcct ttcctgaaa gtttcctat ttattaatt tattgttgc aaaggatca ataccggtg ttaacacgt aatgttctc atatcatcc accagcttg gcaatgtaa taacaatgc agcacttgt tgtctttat tgcaccaga aaaaactaa tttgttgag actgcacta ttaataata aagtaatgg ccttagtga cgtcgtgtg ttttattgt aggtatact gtaacagtc gcagcccat ttccttaat cggcaacca acttcaggt aactgccaa cgtgtctac cagctaaca tcctttctt ccaactacc agaatgcaa aaattgata ttacttagc attactgcg tcagagttc actatagac aaattttaa ctaattttt ttttatttg taagaactt gttgtcttt gattcttca tttttactg ttatttgtg tatttttgg ttgcctaac tctgtattg atttaaatg tgaccacag ttattgtga agaattcat tatcattca tcataaagc aatgtgatc cacgtactt gccggtgtt taatttcag ctaattgtt tagtagttg ttttattta ttgttggtc atattaatt atagttaaa taaagtatt tatttcctt
- >TC003537 RB
gtggaagtc gaacagtcc acctccctc gctgaaata tcctgacat atgcgcgtc gtcgtcggt gttcctgtt ccacaccag ctgcgcaca gtaattccc tgtcagtag cctcaaact gttttaaag tgcatttaa ctcgaagtt attcagttt aagattttt ttatcgcct caagctgtt tgaaacgta atgattttt gaacttaca tgttgtaat atttcgtca ttttttggt gttgtgttg cctttaaat tttccgttc ctttcttat cagactaat aagtgagtt tcggttgcg acctgtaac attacggat taaaactgc tttcattat caagtattc gctgcgata gaggtgcgg gagtgcgag acccaacag tcacgatca tttctttgc ccaagtcag aaacagctt ttgcttcag ctgcaacag gtgtattaa gtgtgtgtt tttaatgcg tgtaaaaaa ttgaaagca acacgtgac ctgtcgaaa tagaattgc tttagggga gggtggtac tcgcatttt tgtagacgt tatgggtgt gttgtaaaa gttattgtg cttccaaaa tcaatatta actattaag gttggaagt taattgagg agaatgccg ctgagtgca gtttgtgtt agtactttg agcagccga cctcgattc tggtcgtct cattcaaat attcgtgct aaagatgtg caacaactc actactatg aacagtgct gaagaacta tcaaaatat aaggagaac atcgatagt tactattct ccaacttta tccagacgc ttgttagga tacggatat gattttccc gagaaatca cccaagaag gagaaaaaa tggtctttg gggagttta tttcgaagg aaaaagaaa gacgaaagt gacagtagc agtgacgac gacctgcag aagaagggt tttttaggt cgaaagaaa cgaaaacct gacaagaaa aaaaagcca aataaacct atcggaacc ttcgaccat gtcgtactt tcccgtaat tcacacctg tactcaaat gggtttaat ggctacgat gaagccggc attttttct gacccgggt aatggtttt ggatacggc agtaaatcg ttaccaaag actcaatcc caggaaatt gtaaaacag cctgttaaa aatgcaaca gttgaggag gtcagcaac tctaggagc acttcgatc tcaggatca acggaagtt ggaagaaaa agccggaaa gggttggca aaagtaagg gcggaggcc agaagggtt aatttgaag cgggacagc agttcggat gaggattct caaagatct gtctcttct actaggttt aggagtgac gaaagtttg ggtcaaggg taccgagat ggctcttta agccgccgc tcaagggcg gcaaggaca gaacgctac ttaaaacga cattctcga gatggcgag aatccaaaa gacttttta agactgtca aaatcggac gccgaaaat tccgtacca cagtggaaa acgtccgat gatagtaac cgatcgtta agtagaagc cctttacta ccaccttca ttaaacaaa accaaaacg atatactcc accttatcg cagtcacct aacatttcg ggattatca actatccct cctagtcat agcaatagt aggattaaa gttagcaat tcaacgagt aacccgtcg tacaagcca cctttgtcg atgaacgac tataactac agtactcga aaactatca aatgattat ctttctaat caacgtagt ttatcttgt gatgccaac atacataaa tccctgcct gtggaagcg gaatcggaa ataattcat gtccagttt cctatagga aaacccaat aacaggtta cggaatctg agtttaatt gagtctcgg catgtaaat gcctgtcat gcaagacaa ccaccacct ccacctccg cgtgatcct agaagatta gtcacggct caatattac gacaatgtg aggcccatc acgtgctat ttagacagc cctatgaaa tcgaaatcg ttccacacg aattcgcaa actcccgca gtgcaaacc aaaaaattc acttcgttt aataaaaac tgcagatcg acttcggaa gatcacata ccacaaaac aacgtccat ttgatacca cgaccatcg tcggcaact cccgacgct tctcaaagg cgcacacaa gttaacaca aatctggaa aattataat tatttaact gataagaaa ccgcggagc agaaaaccg atatttatc cagtcaaat aatagcttg gatagtgtt tcaaccgac aagagtgaa actcagaaa gcgttagat ttctggaaa caaaaggac aaggattcc agaagtgtt aaaaataat gtgaaaacg aagagagcg aaaagtcca caaatgttc acttgccag acgcatgtt caaacccag gtgttccta cctagcgtt cttcagaat gatattacg tctgagaat ataaccaaa tcgatggat actgaaaaa gatgattcg gggacaaac cgttacatg cctgttcta tgccaggag ccgttggct aaagaagag gaaaatgaa gatttgaaa cgcaagtct tcaaatctg gaagaggct cttgacgag ttggaggca atttacaac agcttgcga ctgggcgat gaggacctt ctggaaaga gcggagaag cgggaaaag gatgccgct gagcagaag ttaaaggag gaaggacgc gaagtttat tccaactgg agcacgtcg aggggcgct ttgagcgac tctagtttt agttacgaa cctttcgat tctataggt tcccctaga cgtaagcgt tttgtgcga aaaagccaa actatcgat aggagagcc gacgatatg gctttccga aagttgaat aaagaacgt tcagctaca atcaatgat ccgcaaggt gttttatcc aaaattagt tacctaaac gcgtctcct gtttacgga aaagaacca aaggaacat gaagtgcca aataaaaac tctaaggaa cctgatatt actttagat gacgtggtt tatagaaac gtcaagttt gccaacaat tgcccaaag gttgtggaa cctcaacca ccgttcggg ataccctta gggcccata acaccggct gcgaacagt gattatctc cacgctgtt cccgataat attccgcga ccaatgttt aaaccacgg aaaatccct gatttggtt aaggatgat ttggctttc cgtaacttg aggaaagac cagggcaag gaacctgcc cttccgccc ttatctccg gacgatctt aagaacaat aacgcgtcc gaaccgaag ctagatctc gactcgttg aagaaaaaa cgagccgtg aggtcgctc tccgccaac attggcagc ataatcaat aaaaatttc ttatccaaa ggattagaa aaacacggt ggtgaaagt aacgagtca aaaacatta acagacatt gcggacgcg atggaaatc gcacgccaa gtccttatg gagaaagaa aacaaaatt agtgccacc agaagagca tttcttagc gacaccgac acgaacaac ggtaacgac agcataacc gaaagccgg atgaacttt ttgaacagt ttgaagact ccctcggac cactactta caagcaaaa ccaccgaga ggtgtcacc acacccgaa tgtaaatca ccgagatcg ccgaaagaa tcaactccg attccggtc gaagaatcg agagattcg tctcttgac gatttgctc acagctttg gccaccgaa gctagagaa acgaatgaa cgcatagcc aaagaactg aagagtttt gaagaccgc aaacggaag aaaagccct gctgatgaa aatttgaac aactacgag ctgacgaaa actctgagt gatattgat gcggtgtcg gaacaggcg aaattgtgc gagaaattg ttggagggg gttgtggac agtacggat ttgatggcg tctgcaaag ccagttttg gaagaggtg gggcagatt gataccgaa gctactcgg gaagaatct gttgcaaat gttgtttta acaagagac gattccaaa aacaaggag aaatcgcca gtgaagctg tcgtgcgag tcagatcat gactacgag aacttggtg tctgatcaa gagttgaat cttgacgaa gctccggtt gaagatgtg aagtgcacg tcgccgttc gaagaacac aaggcggaa ttgattgcg actttccaa gagttgaag aacaatgtt agtcttgat ctcgacggc agaaaggaa aatgatacg aatgataaa gaaaacgat gagacggtt gcaggtgag gcgtgtgtg ggtgttgta gatgccgct tccaacgtc acagaatgt gcctgtttc gaaccattt gtgtctaac ccacaaact cacgattct aaccatgat accctctct actaatcac gtgtgctta tcaggcgtc gatagttca aattgccca ggtagctcc gcaggttta gagaaatgc ggtcattta gactcctta gattcgttg aagtcgccc aaattggtt ctagagacg tcgaagttg tcggaacac gaaggaact aatcaaata ttacaaaat caatccatg atttgtcgt gaatctgat tcgaataaa ccggggccc tcttgtgat agcgtaatg gattttaga aggaggagt agcgagagg ggtgaaagt gggcacatt ctcgatgat gacgggaaa ccatctcct gcctggtac cgcgacccc gcaacagtg gctgttgca tgttcttac ggaattgca tgcgcccat cagctggct tctcttgat tttgtggct attttagga cttttgttt gcagtgtta tcttttata gctgctcta atattttaa aaaagttac aaagcatgt tttggttta attcatgtt tccattttc ttgtcattt atttacccc tagtgaaga ctacttagt tgtacaagt ttgtttaag aaatacgat ccaatgcca aatatatta tgttaagtt ctctgtaga ttttgttgt acaaaattg ttttataaa taaaacgtt atagtttct tttaagttt tattttatc ggaacgaac aatgaaagt tgacgaaag ttaaagaac aggattagc aggttcatt attaatttg aatgtgaaa agcatcgac tttcaaatt attattaat tcacgaaag attggaacg agcgtttac gaatgtttt ttagttctt ttattactt tttacgcaa aataattat agttagttt agacaacca tcaatacac atgcgcttt tgttttttt atgatcggt agattatcc attacttca cctggaatg cgttgtttt attaatcta ttagtgttc ttctgttgc ccacttcct ttcctgaaa gtttcctat ttattaatt tattgttgc aaaggatca ataccggtg ttaacacgt aatgttctc atatcatcc accagcttg gcaatgtaa taacaatgc agcacttgt tgtctttat tgcaccaga aaaaactaa tttgttgag actgcacta ttaataata aagtaatgg ccttagtga cgtcgtgtg ttttattgt aggtatact gtaacagtc gcagcccat ttccttaat cggcaacca acttcaggt aactgccaa cgtgtctac cagctaaca tcctttctt ccaactacc agaatgcaa aaattgata ttacttagc attactgcg tcagagttc actatagac aaattttaa ctaattttt ttttatttg taagaactt gttgtcttt gattcttca tttttactg ttatttgtg tatttttgg ttgcctaac tctgtattg atttaaatg tgaccacag ttattgtga agaattcat tatcattca tcataaagc aatgtgatc cacgtactt gccggtgtt taatttcag ctaattgtt tagtagttg ttttattta ttgttggtc atattaatt atagttaaa taaagtatt tatttcctt
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