Status
Please wait ...
Gene information
General information
TC003537 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2049258..2064007
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003537
    iBeetle screen iB_03571
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003537 RA
      MNSAEELSK YKENIDSYY SPTLSRRLL GYGYDFPEK 
      SPKKEKKWS LGSLFRRKK KDESDSSSD DDLQKKGFL 
      GRKKRKPDK KKKPNKPIG TFDHVVLSR NSHLYSNGF 
      NGYDEAGIF SDPGNGFGY GSKSLPKTQ SQEIVKQPV 
      KNATVEEVS NSRSTSISG STEVGRKSR KGLAKVRAE 
      ARRVNLKRD SSSDEDSQR SVSSTRFRS DESLGQGYR 
      DGSLSRRSR AARTERYLK RHSRDGENP KDFLRLSKS 
      DAENSVPQW KTSDDSNRS LSRSPLLPP SLNKTKTIY 
      STLSQSPNI SGLSTIPPS HSNSRIKVS NSTSNPSYK 
      PPLSMNDYN YSTRKLSND YLSNQRSLS CDANIHKSL 
      PVEAESEII HVQFPIGKP NNRLRNLSL IESRHVNAC 
      HARQPPPPP PRDPRRLVT AQYYDNVRP ITCYLDSPM 
      KSKSFHTNS QTPAVQTKK FTSFNKNCR STSEDHIPQ 
      NNVHLIPRP SSATPDASQ RRTQVNTNL ENYNYLTDK 
      KPRSRKPIF IQSNNSLDS VSTDKSETQ KALDFWKQK 
      DKDSRSVKN NVKTKRAKS PQMFTCQTH VQTQVFLPS 
      VLQNDITSE NITKSMDTE KDDSGTNRY MPVLCQEPL 
      AKEEENEDL KRKSSNLEE ALDELEAIY NSLRLGDED 
      LLERAEKRE KDAAEQKLK EEGREVYSN WSTSRGALS 
      DSSFSYEPF DSIGSPRRK RFVRKSQTI DRRADDMAF 
      RKLNKERSA TINDPQGVL SKISYLNAS PVYGKEPKE 
      HEVPNKNSK EPDITLDDV VYRNVKFAN NCPKVVEPQ 
      PPFGIPLGP ITPAANSDY LHAVPDNIP RPMFKPRKI 
      PDLVKDDLA FRNLRKDQG KEPALPPLS PDDLKNNNA 
      SEPKLDLDS LKKKRAVRS LSANIGSII NKNFLSKGL 
      EKHGGESNE SKTLTDIAD AMEIARQVL MEKENKISA 
      TRRAFLSDT DTNNGNDSI TESRMNFLN SLKTPSDHY 
      LQAKPPRGV TTPECKSPR SPKESTPIP VEESRDSSL 
      DDLLTALAT EARETNERI AKELKSFED RKRKKSPAD 
      ENLNNYELT KTLSDIDAV SEQAKLCEK LLEGVVDST 
      DLMASAKPV LEEVGQIDT EATREESVA NVVLTRDDS 
      KNKEKSPVK LSCESDHDY ENLVSDQEL NLDEAPVED 
      VKCTSPFEE HKAELIATF QELKNNVSL DLDGRKEND 
      TNDKENDET VAGEACVGV VDAASNVTE CACFEPFVS 
      NPQTHDSNH DTLSTNHVC LSGVDSSNC PGSSAGLEK 
      CGHLDSLDS LKSPKLVLE TSKLSEHEG TNQILQNQS 
      MICRESDSN KPGPSCDSV MDFRRRSSE RGESGHILD 
      DDGKPSPAW YRDPATVAV ACSYGIACA HQLASLDFV 
      AILGLLFAV LSFIAALIF
    • >TC003537 RB
      MPLSAVCVS TLSSRPRFW SSHSNIRAK DVQQLTTMN 
      SAEELSKYK ENIDSYYSP TLSRRLLGY GYDFPEKSP 
      KKEKKWSLG SLFRRKKKD ESDSSSDDD LQKKGFLGR 
      KKRKPDKKK KPNKPIGTF DHVVLSRNS HLYSNGFNG 
      YDEAGIFSD PGNGFGYGS KSLPKTQSQ EIVKQPVKN 
      ATVEEVSNS RSTSISGST EVGRKSRKG LAKVRAEAR 
      RVNLKRDSS SDEDSQRSV SSTRFRSDE SLGQGYRDG 
      SLSRRSRAA RTERYLKRH SRDGENPKD FLRLSKSDA 
      ENSVPQWKT SDDSNRSLS RSPLLPPSL NKTKTIYST 
      LSQSPNISG LSTIPPSHS NSRIKVSNS TSNPSYKPP 
      LSMNDYNYS TRKLSNDYL SNQRSLSCD ANIHKSLPV 
      EAESEIIHV QFPIGKPNN RLRNLSLIE SRHVNACHA 
      RQPPPPPPR DPRRLVTAQ YYDNVRPIT CYLDSPMKS 
      KSFHTNSQT PAVQTKKFT SFNKNCRST SEDHIPQNN 
      VHLIPRPSS ATPDASQRR TQVNTNLEN YNYLTDKKP 
      RSRKPIFIQ SNNSLDSVS TDKSETQKA LDFWKQKDK 
      DSRSVKNNV KTKRAKSPQ MFTCQTHVQ TQVFLPSVL 
      QNDITSENI TKSMDTEKD DSGTNRYMP VLCQEPLAK 
      EEENEDLKR KSSNLEEAL DELEAIYNS LRLGDEDLL 
      ERAEKREKD AAEQKLKEE GREVYSNWS TSRGALSDS 
      SFSYEPFDS IGSPRRKRF VRKSQTIDR RADDMAFRK 
      LNKERSATI NDPQGVLSK ISYLNASPV YGKEPKEHE 
      VPNKNSKEP DITLDDVVY RNVKFANNC PKVVEPQPP 
      FGIPLGPIT PAANSDYLH AVPDNIPRP MFKPRKIPD 
      LVKDDLAFR NLRKDQGKE PALPPLSPD DLKNNNASE 
      PKLDLDSLK KKRAVRSLS ANIGSIINK NFLSKGLEK 
      HGGESNESK TLTDIADAM EIARQVLME KENKISATR 
      RAFLSDTDT NNGNDSITE SRMNFLNSL KTPSDHYLQ 
      AKPPRGVTT PECKSPRSP KESTPIPVE ESRDSSLDD 
      LLTALATEA RETNERIAK ELKSFEDRK RKKSPADEN 
      LNNYELTKT LSDIDAVSE QAKLCEKLL EGVVDSTDL 
      MASAKPVLE EVGQIDTEA TREESVANV VLTRDDSKN 
      KEKSPVKLS CESDHDYEN LVSDQELNL DEAPVEDVK 
      CTSPFEEHK AELIATFQE LKNNVSLDL DGRKENDTN 
      DKENDETVA GEACVGVVD AASNVTECA CFEPFVSNP 
      QTHDSNHDT LSTNHVCLS GVDSSNCPG SSAGLEKCG 
      HLDSLDSLK SPKLVLETS KLSEHEGTN QILQNQSMI 
      CRESDSNKP GPSCDSVMD FRRRSSERG ESGHILDDD 
      GKPSPAWYR DPATVAVAC SYGIACAHQ LASLDFVAI 
      LGLLFAVLS FIAALIF
    CDS sequence
    • >TC003537 RA
      atgaacagt gctgaagaa ctatcaaaa tataaggag 
      aacatcgat agttactat tctccaact ttatccaga 
      cgcttgtta ggatacgga tatgatttt cccgagaaa 
      tcacccaag aaggagaaa aaatggtct ttggggagt 
      ttatttcga aggaaaaag aaagacgaa agtgacagt 
      agcagtgac gacgacctg cagaagaag ggtttttta 
      ggtcgaaag aaacgaaaa cctgacaag aaaaaaaag 
      ccaaataaa cctatcgga accttcgac catgtcgta 
      ctttcccgt aattcacac ctgtactca aatgggttt 
      aatggctac gatgaagcc ggcattttt tctgacccg 
      ggtaatggt tttggatac ggcagtaaa tcgttacca 
      aagactcaa tcccaggaa attgtaaaa cagcctgtt 
      aaaaatgca acagttgag gaggtcagc aactctagg 
      agcacttcg atctcagga tcaacggaa gttggaaga 
      aaaagccgg aaagggttg gcaaaagta agggcggag 
      gccagaagg gttaatttg aagcgggac agcagttcg 
      gatgaggat tctcaaaga tctgtctct tctactagg 
      tttaggagt gacgaaagt ttgggtcaa gggtaccga 
      gatggctct ttaagccgc cgctcaagg gcggcaagg 
      acagaacgc tacttaaaa cgacattct cgagatggc 
      gagaatcca aaagacttt ttaagactg tcaaaatcg 
      gacgccgaa aattccgta ccacagtgg aaaacgtcc 
      gatgatagt aaccgatcg ttaagtaga agcccttta 
      ctaccacct tcattaaac aaaaccaaa acgatatac 
      tccacctta tcgcagtca cctaacatt tcgggatta 
      tcaactatc cctcctagt catagcaat agtaggatt 
      aaagttagc aattcaacg agtaacccg tcgtacaag 
      ccacctttg tcgatgaac gactataac tacagtact 
      cgaaaacta tcaaatgat tatctttct aatcaacgt 
      agtttatct tgtgatgcc aacatacat aaatccctg 
      cctgtggaa gcggaatcg gaaataatt catgtccag 
      tttcctata ggaaaaccc aataacagg ttacggaat 
      ctgagttta attgagtct cggcatgta aatgcctgt 
      catgcaaga caaccacca cctccacct ccgcgtgat 
      cctagaaga ttagtcacg gctcaatat tacgacaat 
      gtgaggccc atcacgtgc tatttagac agccctatg 
      aaatcgaaa tcgttccac acgaattcg caaactccc 
      gcagtgcaa accaaaaaa ttcacttcg tttaataaa 
      aactgcaga tcgacttcg gaagatcac ataccacaa 
      aacaacgtc catttgata ccacgacca tcgtcggca 
      actcccgac gcttctcaa aggcgcaca caagttaac 
      acaaatctg gaaaattat aattattta actgataag 
      aaaccgcgg agcagaaaa ccgatattt atccagtca 
      aataatagc ttggatagt gtttcaacc gacaagagt 
      gaaactcag aaagcgtta gatttctgg aaacaaaag 
      gacaaggat tccagaagt gttaaaaat aatgtgaaa 
      acgaagaga gcgaaaagt ccacaaatg ttcacttgc 
      cagacgcat gttcaaacc caggtgttc ctacctagc 
      gttcttcag aatgatatt acgtctgag aatataacc 
      aaatcgatg gatactgaa aaagatgat tcggggaca 
      aaccgttac atgcctgtt ctatgccag gagccgttg 
      gctaaagaa gaggaaaat gaagatttg aaacgcaag 
      tcttcaaat ctggaagag gctcttgac gagttggag 
      gcaatttac aacagcttg cgactgggc gatgaggac 
      cttctggaa agagcggag aagcgggaa aaggatgcc 
      gctgagcag aagttaaag gaggaagga cgcgaagtt 
      tattccaac tggagcacg tcgaggggc gctttgagc 
      gactctagt tttagttac gaacctttc gattctata 
      ggttcccct agacgtaag cgttttgtg cgaaaaagc 
      caaactatc gataggaga gccgacgat atggctttc 
      cgaaagttg aataaagaa cgttcagct acaatcaat 
      gatccgcaa ggtgtttta tccaaaatt agttaccta 
      aacgcgtct cctgtttac ggaaaagaa ccaaaggaa 
      catgaagtg ccaaataaa aactctaag gaacctgat 
      attacttta gatgacgtg gtttataga aacgtcaag 
      tttgccaac aattgccca aaggttgtg gaacctcaa 
      ccaccgttc gggataccc ttagggccc ataacaccg 
      gctgcgaac agtgattat ctccacgct gttcccgat 
      aatattccg cgaccaatg tttaaacca cggaaaatc 
      cctgatttg gttaaggat gatttggct ttccgtaac 
      ttgaggaaa gaccagggc aaggaacct gcccttccg 
      cccttatct ccggacgat cttaagaac aataacgcg 
      tccgaaccg aagctagat ctcgactcg ttgaagaaa 
      aaacgagcc gtgaggtcg ctctccgcc aacattggc 
      agcataatc aataaaaat ttcttatcc aaaggatta 
      gaaaaacac ggtggtgaa agtaacgag tcaaaaaca 
      ttaacagac attgcggac gcgatggaa atcgcacgc 
      caagtcctt atggagaaa gaaaacaaa attagtgcc 
      accagaaga gcatttctt agcgacacc gacacgaac 
      aacggtaac gacagcata accgaaagc cggatgaac 
      tttttgaac agtttgaag actccctcg gaccactac 
      ttacaagca aaaccaccg agaggtgtc accacaccc 
      gaatgtaaa tcaccgaga tcgccgaaa gaatcaact 
      ccgattccg gtcgaagaa tcgagagat tcgtctctt 
      gacgatttg ctcacagct ttggccacc gaagctaga 
      gaaacgaat gaacgcata gccaaagaa ctgaagagt 
      tttgaagac cgcaaacgg aagaaaagc cctgctgat 
      gaaaatttg aacaactac gagctgacg aaaactctg 
      agtgatatt gatgcggtg tcggaacag gcgaaattg 
      tgcgagaaa ttgttggag ggggttgtg gacagtacg 
      gatttgatg gcgtctgca aagccagtt ttggaagag 
      gtggggcag attgatacc gaagctact cgggaagaa 
      tctgttgca aatgttgtt ttaacaaga gacgattcc 
      aaaaacaag gagaaatcg ccagtgaag ctgtcgtgc 
      gagtcagat catgactac gagaacttg gtgtctgat 
      caagagttg aatcttgac gaagctccg gttgaagat 
      gtgaagtgc acgtcgccg ttcgaagaa cacaaggcg 
      gaattgatt gcgactttc caagagttg aagaacaat 
      gttagtctt gatctcgac ggcagaaag gaaaatgat 
      acgaatgat aaagaaaac gatgagacg gttgcaggt 
      gaggcgtgt gtgggtgtt gtagatgcc gcttccaac 
      gtcacagaa tgtgcctgt ttcgaacca tttgtgtct 
      aacccacaa actcacgat tctaaccat gataccctc 
      tctactaat cacgtgtgc ttatcaggc gtcgatagt 
      tcaaattgc ccaggtagc tccgcaggt ttagagaaa 
      tgcggtcat ttagactcc ttagattcg ttgaagtcg 
      cccaaattg gttctagag acgtcgaag ttgtcggaa 
      cacgaagga actaatcaa atattacaa aatcaatcc 
      atgatttgt cgtgaatct gattcgaat aaaccgggg 
      ccctcttgt gatagcgta atggatttt agaaggagg 
      agtagcgag aggggtgaa agtgggcac attctcgat 
      gatgacggg aaaccatct cctgcctgg taccgcgac 
      cccgcaaca gtggctgtt gcatgttct tacggaatt 
      gcatgcgcc catcagctg gcttctctt gattttgtg 
      gctatttta ggacttttg tttgcagtg ttatctttt 
      atagctgct ctaatattt taa
    • >TC003537 RB
      atgccgctg agtgcagtt tgtgttagt actttgagc 
      agccgacct cgattctgg tcgtctcat tcaaatatt 
      cgtgctaaa gatgtgcaa caactcact actatgaac 
      agtgctgaa gaactatca aaatataag gagaacatc 
      gatagttac tattctcca actttatcc agacgcttg 
      ttaggatac ggatatgat tttcccgag aaatcaccc 
      aagaaggag aaaaaatgg tctttgggg agtttattt 
      cgaaggaaa aagaaagac gaaagtgac agtagcagt 
      gacgacgac ctgcagaag aagggtttt ttaggtcga 
      aagaaacga aaacctgac aagaaaaaa aagccaaat 
      aaacctatc ggaaccttc gaccatgtc gtactttcc 
      cgtaattca cacctgtac tcaaatggg tttaatggc 
      tacgatgaa gccggcatt ttttctgac ccgggtaat 
      ggttttgga tacggcagt aaatcgtta ccaaagact 
      caatcccag gaaattgta aaacagcct gttaaaaat 
      gcaacagtt gaggaggtc agcaactct aggagcact 
      tcgatctca ggatcaacg gaagttgga agaaaaagc 
      cggaaaggg ttggcaaaa gtaagggcg gaggccaga 
      agggttaat ttgaagcgg gacagcagt tcggatgag 
      gattctcaa agatctgtc tcttctact aggtttagg 
      agtgacgaa agtttgggt caagggtac cgagatggc 
      tctttaagc cgccgctca agggcggca aggacagaa 
      cgctactta aaacgacat tctcgagat ggcgagaat 
      ccaaaagac tttttaaga ctgtcaaaa tcggacgcc 
      gaaaattcc gtaccacag tggaaaacg tccgatgat 
      agtaaccga tcgttaagt agaagccct ttactacca 
      ccttcatta aacaaaacc aaaacgata tactccacc 
      ttatcgcag tcacctaac atttcggga ttatcaact 
      atccctcct agtcatagc aatagtagg attaaagtt 
      agcaattca acgagtaac ccgtcgtac aagccacct 
      ttgtcgatg aacgactat aactacagt actcgaaaa 
      ctatcaaat gattatctt tctaatcaa cgtagttta 
      tcttgtgat gccaacata cataaatcc ctgcctgtg 
      gaagcggaa tcggaaata attcatgtc cagtttcct 
      ataggaaaa cccaataac aggttacgg aatctgagt 
      ttaattgag tctcggcat gtaaatgcc tgtcatgca 
      agacaacca ccacctcca cctccgcgt gatcctaga 
      agattagtc acggctcaa tattacgac aatgtgagg 
      cccatcacg tgctattta gacagccct atgaaatcg 
      aaatcgttc cacacgaat tcgcaaact cccgcagtg 
      caaaccaaa aaattcact tcgtttaat aaaaactgc 
      agatcgact tcggaagat cacatacca caaaacaac 
      gtccatttg ataccacga ccatcgtcg gcaactccc 
      gacgcttct caaaggcgc acacaagtt aacacaaat 
      ctggaaaat tataattat ttaactgat aagaaaccg 
      cggagcaga aaaccgata tttatccag tcaaataat 
      agcttggat agtgtttca accgacaag agtgaaact 
      cagaaagcg ttagatttc tggaaacaa aaggacaag 
      gattccaga agtgttaaa aataatgtg aaaacgaag 
      agagcgaaa agtccacaa atgttcact tgccagacg 
      catgttcaa acccaggtg ttcctacct agcgttctt 
      cagaatgat attacgtct gagaatata accaaatcg 
      atggatact gaaaaagat gattcgggg acaaaccgt 
      tacatgcct gttctatgc caggagccg ttggctaaa 
      gaagaggaa aatgaagat ttgaaacgc aagtcttca 
      aatctggaa gaggctctt gacgagttg gaggcaatt 
      tacaacagc ttgcgactg ggcgatgag gaccttctg 
      gaaagagcg gagaagcgg gaaaaggat gccgctgag 
      cagaagtta aaggaggaa ggacgcgaa gtttattcc 
      aactggagc acgtcgagg ggcgctttg agcgactct 
      agttttagt tacgaacct ttcgattct ataggttcc 
      cctagacgt aagcgtttt gtgcgaaaa agccaaact 
      atcgatagg agagccgac gatatggct ttccgaaag 
      ttgaataaa gaacgttca gctacaatc aatgatccg 
      caaggtgtt ttatccaaa attagttac ctaaacgcg 
      tctcctgtt tacggaaaa gaaccaaag gaacatgaa 
      gtgccaaat aaaaactct aaggaacct gatattact 
      ttagatgac gtggtttat agaaacgtc aagtttgcc 
      aacaattgc ccaaaggtt gtggaacct caaccaccg 
      ttcgggata cccttaggg cccataaca ccggctgcg 
      aacagtgat tatctccac gctgttccc gataatatt 
      ccgcgacca atgtttaaa ccacggaaa atccctgat 
      ttggttaag gatgatttg gctttccgt aacttgagg 
      aaagaccag ggcaaggaa cctgccctt ccgccctta 
      tctccggac gatcttaag aacaataac gcgtccgaa 
      ccgaagcta gatctcgac tcgttgaag aaaaaacga 
      gccgtgagg tcgctctcc gccaacatt ggcagcata 
      atcaataaa aatttctta tccaaagga ttagaaaaa 
      cacggtggt gaaagtaac gagtcaaaa acattaaca 
      gacattgcg gacgcgatg gaaatcgca cgccaagtc 
      cttatggag aaagaaaac aaaattagt gccaccaga 
      agagcattt cttagcgac accgacacg aacaacggt 
      aacgacagc ataaccgaa agccggatg aactttttg 
      aacagtttg aagactccc tcggaccac tacttacaa 
      gcaaaacca ccgagaggt gtcaccaca cccgaatgt 
      aaatcaccg agatcgccg aaagaatca actccgatt 
      ccggtcgaa gaatcgaga gattcgtct cttgacgat 
      ttgctcaca gctttggcc accgaagct agagaaacg 
      aatgaacgc atagccaaa gaactgaag agttttgaa 
      gaccgcaaa cggaagaaa agccctgct gatgaaaat 
      ttgaacaac tacgagctg acgaaaact ctgagtgat 
      attgatgcg gtgtcggaa caggcgaaa ttgtgcgag 
      aaattgttg gagggggtt gtggacagt acggatttg 
      atggcgtct gcaaagcca gttttggaa gaggtgggg 
      cagattgat accgaagct actcgggaa gaatctgtt 
      gcaaatgtt gttttaaca agagacgat tccaaaaac 
      aaggagaaa tcgccagtg aagctgtcg tgcgagtca 
      gatcatgac tacgagaac ttggtgtct gatcaagag 
      ttgaatctt gacgaagct ccggttgaa gatgtgaag 
      tgcacgtcg ccgttcgaa gaacacaag gcggaattg 
      attgcgact ttccaagag ttgaagaac aatgttagt 
      cttgatctc gacggcaga aaggaaaat gatacgaat 
      gataaagaa aacgatgag acggttgca ggtgaggcg 
      tgtgtgggt gttgtagat gccgcttcc aacgtcaca 
      gaatgtgcc tgtttcgaa ccatttgtg tctaaccca 
      caaactcac gattctaac catgatacc ctctctact 
      aatcacgtg tgcttatca ggcgtcgat agttcaaat 
      tgcccaggt agctccgca ggtttagag aaatgcggt 
      catttagac tccttagat tcgttgaag tcgcccaaa 
      ttggttcta gagacgtcg aagttgtcg gaacacgaa 
      ggaactaat caaatatta caaaatcaa tccatgatt 
      tgtcgtgaa tctgattcg aataaaccg gggccctct 
      tgtgatagc gtaatggat tttagaagg aggagtagc 
      gagaggggt gaaagtggg cacattctc gatgatgac 
      gggaaacca tctcctgcc tggtaccgc gaccccgca 
      acagtggct gttgcatgt tcttacgga attgcatgc 
      gcccatcag ctggcttct cttgatttt gtggctatt 
      ttaggactt ttgtttgca gtgttatct tttatagct 
      gctctaata ttttaa
    mRNA sequence
    • >TC003537 RA
      gtggaagtc gaacagtcc acctccctc gctgaaata 
      tcctgacat atgcgcgtc gtcgtcggt gttcctgtt 
      ccacaccag ctgcgcaca gtaattccc tgtcagtag 
      cctcaaact gttttaaag tgcatttaa ctcgaagtt 
      attcagttt aagattttt ttatcgcct caagctgtt 
      tgaaacgta atgattttt gaacttaca tgttgtaat 
      atttcgtca ttttttggt gttgtgttg cctttaaat 
      tttccgttc ctttcttat cagactaat aagtgagtt 
      tcggttgcg acctgtaac attacggat taaaactgc 
      tttcattat caaactttg agcagccga cctcgattc 
      tggtcgtct cattcaaat attcgtgct aaagatgtg 
      caacaactc actactatg aacagtgct gaagaacta 
      tcaaaatat aaggagaac atcgatagt tactattct 
      ccaacttta tccagacgc ttgttagga tacggatat 
      gattttccc gagaaatca cccaagaag gagaaaaaa 
      tggtctttg gggagttta tttcgaagg aaaaagaaa 
      gacgaaagt gacagtagc agtgacgac gacctgcag 
      aagaagggt tttttaggt cgaaagaaa cgaaaacct 
      gacaagaaa aaaaagcca aataaacct atcggaacc 
      ttcgaccat gtcgtactt tcccgtaat tcacacctg 
      tactcaaat gggtttaat ggctacgat gaagccggc 
      attttttct gacccgggt aatggtttt ggatacggc 
      agtaaatcg ttaccaaag actcaatcc caggaaatt 
      gtaaaacag cctgttaaa aatgcaaca gttgaggag 
      gtcagcaac tctaggagc acttcgatc tcaggatca 
      acggaagtt ggaagaaaa agccggaaa gggttggca 
      aaagtaagg gcggaggcc agaagggtt aatttgaag 
      cgggacagc agttcggat gaggattct caaagatct 
      gtctcttct actaggttt aggagtgac gaaagtttg 
      ggtcaaggg taccgagat ggctcttta agccgccgc 
      tcaagggcg gcaaggaca gaacgctac ttaaaacga 
      cattctcga gatggcgag aatccaaaa gacttttta 
      agactgtca aaatcggac gccgaaaat tccgtacca 
      cagtggaaa acgtccgat gatagtaac cgatcgtta 
      agtagaagc cctttacta ccaccttca ttaaacaaa 
      accaaaacg atatactcc accttatcg cagtcacct 
      aacatttcg ggattatca actatccct cctagtcat 
      agcaatagt aggattaaa gttagcaat tcaacgagt 
      aacccgtcg tacaagcca cctttgtcg atgaacgac 
      tataactac agtactcga aaactatca aatgattat 
      ctttctaat caacgtagt ttatcttgt gatgccaac 
      atacataaa tccctgcct gtggaagcg gaatcggaa 
      ataattcat gtccagttt cctatagga aaacccaat 
      aacaggtta cggaatctg agtttaatt gagtctcgg 
      catgtaaat gcctgtcat gcaagacaa ccaccacct 
      ccacctccg cgtgatcct agaagatta gtcacggct 
      caatattac gacaatgtg aggcccatc acgtgctat 
      ttagacagc cctatgaaa tcgaaatcg ttccacacg 
      aattcgcaa actcccgca gtgcaaacc aaaaaattc 
      acttcgttt aataaaaac tgcagatcg acttcggaa 
      gatcacata ccacaaaac aacgtccat ttgatacca 
      cgaccatcg tcggcaact cccgacgct tctcaaagg 
      cgcacacaa gttaacaca aatctggaa aattataat 
      tatttaact gataagaaa ccgcggagc agaaaaccg 
      atatttatc cagtcaaat aatagcttg gatagtgtt 
      tcaaccgac aagagtgaa actcagaaa gcgttagat 
      ttctggaaa caaaaggac aaggattcc agaagtgtt 
      aaaaataat gtgaaaacg aagagagcg aaaagtcca 
      caaatgttc acttgccag acgcatgtt caaacccag 
      gtgttccta cctagcgtt cttcagaat gatattacg 
      tctgagaat ataaccaaa tcgatggat actgaaaaa 
      gatgattcg gggacaaac cgttacatg cctgttcta 
      tgccaggag ccgttggct aaagaagag gaaaatgaa 
      gatttgaaa cgcaagtct tcaaatctg gaagaggct 
      cttgacgag ttggaggca atttacaac agcttgcga 
      ctgggcgat gaggacctt ctggaaaga gcggagaag 
      cgggaaaag gatgccgct gagcagaag ttaaaggag 
      gaaggacgc gaagtttat tccaactgg agcacgtcg 
      aggggcgct ttgagcgac tctagtttt agttacgaa 
      cctttcgat tctataggt tcccctaga cgtaagcgt 
      tttgtgcga aaaagccaa actatcgat aggagagcc 
      gacgatatg gctttccga aagttgaat aaagaacgt 
      tcagctaca atcaatgat ccgcaaggt gttttatcc 
      aaaattagt tacctaaac gcgtctcct gtttacgga 
      aaagaacca aaggaacat gaagtgcca aataaaaac 
      tctaaggaa cctgatatt actttagat gacgtggtt 
      tatagaaac gtcaagttt gccaacaat tgcccaaag 
      gttgtggaa cctcaacca ccgttcggg ataccctta 
      gggcccata acaccggct gcgaacagt gattatctc 
      cacgctgtt cccgataat attccgcga ccaatgttt 
      aaaccacgg aaaatccct gatttggtt aaggatgat 
      ttggctttc cgtaacttg aggaaagac cagggcaag 
      gaacctgcc cttccgccc ttatctccg gacgatctt 
      aagaacaat aacgcgtcc gaaccgaag ctagatctc 
      gactcgttg aagaaaaaa cgagccgtg aggtcgctc 
      tccgccaac attggcagc ataatcaat aaaaatttc 
      ttatccaaa ggattagaa aaacacggt ggtgaaagt 
      aacgagtca aaaacatta acagacatt gcggacgcg 
      atggaaatc gcacgccaa gtccttatg gagaaagaa 
      aacaaaatt agtgccacc agaagagca tttcttagc 
      gacaccgac acgaacaac ggtaacgac agcataacc 
      gaaagccgg atgaacttt ttgaacagt ttgaagact 
      ccctcggac cactactta caagcaaaa ccaccgaga 
      ggtgtcacc acacccgaa tgtaaatca ccgagatcg 
      ccgaaagaa tcaactccg attccggtc gaagaatcg 
      agagattcg tctcttgac gatttgctc acagctttg 
      gccaccgaa gctagagaa acgaatgaa cgcatagcc 
      aaagaactg aagagtttt gaagaccgc aaacggaag 
      aaaagccct gctgatgaa aatttgaac aactacgag 
      ctgacgaaa actctgagt gatattgat gcggtgtcg 
      gaacaggcg aaattgtgc gagaaattg ttggagggg 
      gttgtggac agtacggat ttgatggcg tctgcaaag 
      ccagttttg gaagaggtg gggcagatt gataccgaa 
      gctactcgg gaagaatct gttgcaaat gttgtttta 
      acaagagac gattccaaa aacaaggag aaatcgcca 
      gtgaagctg tcgtgcgag tcagatcat gactacgag 
      aacttggtg tctgatcaa gagttgaat cttgacgaa 
      gctccggtt gaagatgtg aagtgcacg tcgccgttc 
      gaagaacac aaggcggaa ttgattgcg actttccaa 
      gagttgaag aacaatgtt agtcttgat ctcgacggc 
      agaaaggaa aatgatacg aatgataaa gaaaacgat 
      gagacggtt gcaggtgag gcgtgtgtg ggtgttgta 
      gatgccgct tccaacgtc acagaatgt gcctgtttc 
      gaaccattt gtgtctaac ccacaaact cacgattct 
      aaccatgat accctctct actaatcac gtgtgctta 
      tcaggcgtc gatagttca aattgccca ggtagctcc 
      gcaggttta gagaaatgc ggtcattta gactcctta 
      gattcgttg aagtcgccc aaattggtt ctagagacg 
      tcgaagttg tcggaacac gaaggaact aatcaaata 
      ttacaaaat caatccatg atttgtcgt gaatctgat 
      tcgaataaa ccggggccc tcttgtgat agcgtaatg 
      gattttaga aggaggagt agcgagagg ggtgaaagt 
      gggcacatt ctcgatgat gacgggaaa ccatctcct 
      gcctggtac cgcgacccc gcaacagtg gctgttgca 
      tgttcttac ggaattgca tgcgcccat cagctggct 
      tctcttgat tttgtggct attttagga cttttgttt 
      gcagtgtta tcttttata gctgctcta atattttaa 
      aaaagttac aaagcatgt tttggttta attcatgtt 
      tccattttc ttgtcattt atttacccc tagtgaaga 
      ctacttagt tgtacaagt ttgtttaag aaatacgat 
      ccaatgcca aatatatta tgttaagtt ctctgtaga 
      ttttgttgt acaaaattg ttttataaa taaaacgtt 
      atagtttct tttaagttt tattttatc ggaacgaac 
      aatgaaagt tgacgaaag ttaaagaac aggattagc 
      aggttcatt attaatttg aatgtgaaa agcatcgac 
      tttcaaatt attattaat tcacgaaag attggaacg 
      agcgtttac gaatgtttt ttagttctt ttattactt 
      tttacgcaa aataattat agttagttt agacaacca 
      tcaatacac atgcgcttt tgttttttt atgatcggt 
      agattatcc attacttca cctggaatg cgttgtttt 
      attaatcta ttagtgttc ttctgttgc ccacttcct 
      ttcctgaaa gtttcctat ttattaatt tattgttgc 
      aaaggatca ataccggtg ttaacacgt aatgttctc 
      atatcatcc accagcttg gcaatgtaa taacaatgc 
      agcacttgt tgtctttat tgcaccaga aaaaactaa 
      tttgttgag actgcacta ttaataata aagtaatgg 
      ccttagtga cgtcgtgtg ttttattgt aggtatact 
      gtaacagtc gcagcccat ttccttaat cggcaacca 
      acttcaggt aactgccaa cgtgtctac cagctaaca 
      tcctttctt ccaactacc agaatgcaa aaattgata 
      ttacttagc attactgcg tcagagttc actatagac 
      aaattttaa ctaattttt ttttatttg taagaactt 
      gttgtcttt gattcttca tttttactg ttatttgtg 
      tatttttgg ttgcctaac tctgtattg atttaaatg 
      tgaccacag ttattgtga agaattcat tatcattca 
      tcataaagc aatgtgatc cacgtactt gccggtgtt 
      taatttcag ctaattgtt tagtagttg ttttattta 
      ttgttggtc atattaatt atagttaaa taaagtatt 
      tatttcctt
    • >TC003537 RB
      gtggaagtc gaacagtcc acctccctc gctgaaata 
      tcctgacat atgcgcgtc gtcgtcggt gttcctgtt 
      ccacaccag ctgcgcaca gtaattccc tgtcagtag 
      cctcaaact gttttaaag tgcatttaa ctcgaagtt 
      attcagttt aagattttt ttatcgcct caagctgtt 
      tgaaacgta atgattttt gaacttaca tgttgtaat 
      atttcgtca ttttttggt gttgtgttg cctttaaat 
      tttccgttc ctttcttat cagactaat aagtgagtt 
      tcggttgcg acctgtaac attacggat taaaactgc 
      tttcattat caagtattc gctgcgata gaggtgcgg 
      gagtgcgag acccaacag tcacgatca tttctttgc 
      ccaagtcag aaacagctt ttgcttcag ctgcaacag 
      gtgtattaa gtgtgtgtt tttaatgcg tgtaaaaaa 
      ttgaaagca acacgtgac ctgtcgaaa tagaattgc 
      tttagggga gggtggtac tcgcatttt tgtagacgt 
      tatgggtgt gttgtaaaa gttattgtg cttccaaaa 
      tcaatatta actattaag gttggaagt taattgagg 
      agaatgccg ctgagtgca gtttgtgtt agtactttg 
      agcagccga cctcgattc tggtcgtct cattcaaat 
      attcgtgct aaagatgtg caacaactc actactatg 
      aacagtgct gaagaacta tcaaaatat aaggagaac 
      atcgatagt tactattct ccaacttta tccagacgc 
      ttgttagga tacggatat gattttccc gagaaatca 
      cccaagaag gagaaaaaa tggtctttg gggagttta 
      tttcgaagg aaaaagaaa gacgaaagt gacagtagc 
      agtgacgac gacctgcag aagaagggt tttttaggt 
      cgaaagaaa cgaaaacct gacaagaaa aaaaagcca 
      aataaacct atcggaacc ttcgaccat gtcgtactt 
      tcccgtaat tcacacctg tactcaaat gggtttaat 
      ggctacgat gaagccggc attttttct gacccgggt 
      aatggtttt ggatacggc agtaaatcg ttaccaaag 
      actcaatcc caggaaatt gtaaaacag cctgttaaa 
      aatgcaaca gttgaggag gtcagcaac tctaggagc 
      acttcgatc tcaggatca acggaagtt ggaagaaaa 
      agccggaaa gggttggca aaagtaagg gcggaggcc 
      agaagggtt aatttgaag cgggacagc agttcggat 
      gaggattct caaagatct gtctcttct actaggttt 
      aggagtgac gaaagtttg ggtcaaggg taccgagat 
      ggctcttta agccgccgc tcaagggcg gcaaggaca 
      gaacgctac ttaaaacga cattctcga gatggcgag 
      aatccaaaa gacttttta agactgtca aaatcggac 
      gccgaaaat tccgtacca cagtggaaa acgtccgat 
      gatagtaac cgatcgtta agtagaagc cctttacta 
      ccaccttca ttaaacaaa accaaaacg atatactcc 
      accttatcg cagtcacct aacatttcg ggattatca 
      actatccct cctagtcat agcaatagt aggattaaa 
      gttagcaat tcaacgagt aacccgtcg tacaagcca 
      cctttgtcg atgaacgac tataactac agtactcga 
      aaactatca aatgattat ctttctaat caacgtagt 
      ttatcttgt gatgccaac atacataaa tccctgcct 
      gtggaagcg gaatcggaa ataattcat gtccagttt 
      cctatagga aaacccaat aacaggtta cggaatctg 
      agtttaatt gagtctcgg catgtaaat gcctgtcat 
      gcaagacaa ccaccacct ccacctccg cgtgatcct 
      agaagatta gtcacggct caatattac gacaatgtg 
      aggcccatc acgtgctat ttagacagc cctatgaaa 
      tcgaaatcg ttccacacg aattcgcaa actcccgca 
      gtgcaaacc aaaaaattc acttcgttt aataaaaac 
      tgcagatcg acttcggaa gatcacata ccacaaaac 
      aacgtccat ttgatacca cgaccatcg tcggcaact 
      cccgacgct tctcaaagg cgcacacaa gttaacaca 
      aatctggaa aattataat tatttaact gataagaaa 
      ccgcggagc agaaaaccg atatttatc cagtcaaat 
      aatagcttg gatagtgtt tcaaccgac aagagtgaa 
      actcagaaa gcgttagat ttctggaaa caaaaggac 
      aaggattcc agaagtgtt aaaaataat gtgaaaacg 
      aagagagcg aaaagtcca caaatgttc acttgccag 
      acgcatgtt caaacccag gtgttccta cctagcgtt 
      cttcagaat gatattacg tctgagaat ataaccaaa 
      tcgatggat actgaaaaa gatgattcg gggacaaac 
      cgttacatg cctgttcta tgccaggag ccgttggct 
      aaagaagag gaaaatgaa gatttgaaa cgcaagtct 
      tcaaatctg gaagaggct cttgacgag ttggaggca 
      atttacaac agcttgcga ctgggcgat gaggacctt 
      ctggaaaga gcggagaag cgggaaaag gatgccgct 
      gagcagaag ttaaaggag gaaggacgc gaagtttat 
      tccaactgg agcacgtcg aggggcgct ttgagcgac 
      tctagtttt agttacgaa cctttcgat tctataggt 
      tcccctaga cgtaagcgt tttgtgcga aaaagccaa 
      actatcgat aggagagcc gacgatatg gctttccga 
      aagttgaat aaagaacgt tcagctaca atcaatgat 
      ccgcaaggt gttttatcc aaaattagt tacctaaac 
      gcgtctcct gtttacgga aaagaacca aaggaacat 
      gaagtgcca aataaaaac tctaaggaa cctgatatt 
      actttagat gacgtggtt tatagaaac gtcaagttt 
      gccaacaat tgcccaaag gttgtggaa cctcaacca 
      ccgttcggg ataccctta gggcccata acaccggct 
      gcgaacagt gattatctc cacgctgtt cccgataat 
      attccgcga ccaatgttt aaaccacgg aaaatccct 
      gatttggtt aaggatgat ttggctttc cgtaacttg 
      aggaaagac cagggcaag gaacctgcc cttccgccc 
      ttatctccg gacgatctt aagaacaat aacgcgtcc 
      gaaccgaag ctagatctc gactcgttg aagaaaaaa 
      cgagccgtg aggtcgctc tccgccaac attggcagc 
      ataatcaat aaaaatttc ttatccaaa ggattagaa 
      aaacacggt ggtgaaagt aacgagtca aaaacatta 
      acagacatt gcggacgcg atggaaatc gcacgccaa 
      gtccttatg gagaaagaa aacaaaatt agtgccacc 
      agaagagca tttcttagc gacaccgac acgaacaac 
      ggtaacgac agcataacc gaaagccgg atgaacttt 
      ttgaacagt ttgaagact ccctcggac cactactta 
      caagcaaaa ccaccgaga ggtgtcacc acacccgaa 
      tgtaaatca ccgagatcg ccgaaagaa tcaactccg 
      attccggtc gaagaatcg agagattcg tctcttgac 
      gatttgctc acagctttg gccaccgaa gctagagaa 
      acgaatgaa cgcatagcc aaagaactg aagagtttt 
      gaagaccgc aaacggaag aaaagccct gctgatgaa 
      aatttgaac aactacgag ctgacgaaa actctgagt 
      gatattgat gcggtgtcg gaacaggcg aaattgtgc 
      gagaaattg ttggagggg gttgtggac agtacggat 
      ttgatggcg tctgcaaag ccagttttg gaagaggtg 
      gggcagatt gataccgaa gctactcgg gaagaatct 
      gttgcaaat gttgtttta acaagagac gattccaaa 
      aacaaggag aaatcgcca gtgaagctg tcgtgcgag 
      tcagatcat gactacgag aacttggtg tctgatcaa 
      gagttgaat cttgacgaa gctccggtt gaagatgtg 
      aagtgcacg tcgccgttc gaagaacac aaggcggaa 
      ttgattgcg actttccaa gagttgaag aacaatgtt 
      agtcttgat ctcgacggc agaaaggaa aatgatacg 
      aatgataaa gaaaacgat gagacggtt gcaggtgag 
      gcgtgtgtg ggtgttgta gatgccgct tccaacgtc 
      acagaatgt gcctgtttc gaaccattt gtgtctaac 
      ccacaaact cacgattct aaccatgat accctctct 
      actaatcac gtgtgctta tcaggcgtc gatagttca 
      aattgccca ggtagctcc gcaggttta gagaaatgc 
      ggtcattta gactcctta gattcgttg aagtcgccc 
      aaattggtt ctagagacg tcgaagttg tcggaacac 
      gaaggaact aatcaaata ttacaaaat caatccatg 
      atttgtcgt gaatctgat tcgaataaa ccggggccc 
      tcttgtgat agcgtaatg gattttaga aggaggagt 
      agcgagagg ggtgaaagt gggcacatt ctcgatgat 
      gacgggaaa ccatctcct gcctggtac cgcgacccc 
      gcaacagtg gctgttgca tgttcttac ggaattgca 
      tgcgcccat cagctggct tctcttgat tttgtggct 
      attttagga cttttgttt gcagtgtta tcttttata 
      gctgctcta atattttaa aaaagttac aaagcatgt 
      tttggttta attcatgtt tccattttc ttgtcattt 
      atttacccc tagtgaaga ctacttagt tgtacaagt 
      ttgtttaag aaatacgat ccaatgcca aatatatta 
      tgttaagtt ctctgtaga ttttgttgt acaaaattg 
      ttttataaa taaaacgtt atagtttct tttaagttt 
      tattttatc ggaacgaac aatgaaagt tgacgaaag 
      ttaaagaac aggattagc aggttcatt attaatttg 
      aatgtgaaa agcatcgac tttcaaatt attattaat 
      tcacgaaag attggaacg agcgtttac gaatgtttt 
      ttagttctt ttattactt tttacgcaa aataattat 
      agttagttt agacaacca tcaatacac atgcgcttt 
      tgttttttt atgatcggt agattatcc attacttca 
      cctggaatg cgttgtttt attaatcta ttagtgttc 
      ttctgttgc ccacttcct ttcctgaaa gtttcctat 
      ttattaatt tattgttgc aaaggatca ataccggtg 
      ttaacacgt aatgttctc atatcatcc accagcttg 
      gcaatgtaa taacaatgc agcacttgt tgtctttat 
      tgcaccaga aaaaactaa tttgttgag actgcacta 
      ttaataata aagtaatgg ccttagtga cgtcgtgtg 
      ttttattgt aggtatact gtaacagtc gcagcccat 
      ttccttaat cggcaacca acttcaggt aactgccaa 
      cgtgtctac cagctaaca tcctttctt ccaactacc 
      agaatgcaa aaattgata ttacttagc attactgcg 
      tcagagttc actatagac aaattttaa ctaattttt 
      ttttatttg taagaactt gttgtcttt gattcttca 
      tttttactg ttatttgtg tatttttgg ttgcctaac 
      tctgtattg atttaaatg tgaccacag ttattgtga 
      agaattcat tatcattca tcataaagc aatgtgatc 
      cacgtactt gccggtgtt taatttcag ctaattgtt 
      tagtagttg ttttattta ttgttggtc atattaatt 
      atagttaaa taaagtatt tatttcctt