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Gene information
General information
TC003528 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1700095..1702159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003528
    iBeetle screen iB_03570
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection

    Comment: younger than L7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003528 RA
      MVKTSDQKC VKVVVLGDF GRSPRMQYH CMSLSEMGH 
      RVDVIAYGG TELLDSIKA DPFIFYHYL LPVPQLPIK 
      LLNYAFKTV MQAINLLFL LLITTRSDI LIMQNPPAI 
      PAVVICWLY TRIVNAKFV IDWHNYAHT IMALSVGER 
      NPLVKITKK VEVVVGRRA DHNFCVTKA MRDDLQKNW 
      NIKATTLYD RPPLIFKPL ELEEKHKFL LECGKKYNK 
      IFLDAHENS TVFTEKTEE GIKLKSERP GLLVSSTSW 
      TEDEDFSIL LTALQEYEK QCHEGNARN LPKLICVIT 
      GKGPLKDYY LDRISKLTW DYVTIITPW LDSEDYPKI 
      LASADLGVS LHTSSSGLD LPMKVVDMF GCGLPVCAF 
      DFKCLNELV KDGENSFIF KSPEELSRH VLNWFENFP 
      SNENQKRTA SKFKTELQS FQNLRWKEN WELVASPVF 
      K
    CDS sequence
    • >TC003528 RA
      atggttaaa acgagtgac cagaaatgt gtgaaagtg 
      gtggttttg ggggacttt gggcggagc cccaggatg 
      cagtaccac tgcatgtcc ctgtcagaa atggggcac 
      cgcgtggac gtgattgct tacggagga acggagctc 
      cttgactcc atcaaagca gaccccttc attttctac 
      cactatctg ctcccagtg ccccaactt cccatcaag 
      ctactcaat tacgcattc aagacagtt atgcaagcg 
      attaattta ctatttctg cttcttatc acgaccaga 
      tcagatatt ttaattatg caaaacccg cctgcaatt 
      cccgcagtg gtaatctgc tggttgtac acacgaatt 
      gtaaatgcg aaatttgtg atagattgg cataactat 
      gctcacact atcatggcc ctgagtgtc ggggagcga 
      aaccctctg gttaaaatt acgaaaaaa gtggaagtt 
      gttgtgggc cgcagagct gatcataat ttttgcgtc 
      accaaagca atgagagac gatttgcaa aaaaactgg 
      aacataaaa gccacaact ttatacgac aggccgcct 
      ttaatattt aaaccactt gagttggaa gaaaaacac 
      aaatttttg ttagagtgc ggcaaaaag tacaataaa 
      atattcttg gacgcccac gaaaactca acggttttc 
      actgagaaa acagaagag ggcattaaa ttaaaaagc 
      gagagaccg ggattgcta gtttccagc actagttgg 
      actgaagat gaggatttt tccatcttg ttaacagcc 
      ttacaagag tacgaaaaa cagtgtcat gaaggcaat 
      gcaagaaat ttgcctaaa ttgatttgt gtgataaca 
      ggaaagggg ccgttaaaa gactactat cttgacagg 
      atttccaaa ctgacttgg gattacgtt actataata 
      accccctgg ttagattct gaagactat ccaaaaatt 
      ttagcttca gcagatttg ggagtctct cttcacaca 
      agttctagt ggtcttgac cttcccatg aaagtggtt 
      gatatgttt ggttgtggt ttaccagtc tgtgccttt 
      gactttaaa tgcctaaat gaactagta aaagacgga 
      gaaaacagt ttcatattc aaatcccct gaagagtta 
      tcccggcac gtgttaaat tggtttgaa aacttccca 
      agcaacgag aaccaaaaa cgcactgcg tcaaaattc 
      aagactgaa ctccaatca ttccaaaac ctgcgatgg 
      aaggaaaat tgggaattg gtggcgtca ccagttttc 
      aaataa
    mRNA sequence
    • >TC003528 RA
      ttggggtta gaaatggac tggaaattt atttactct 
      caaaaataa acattaaaa tttctcatt tattgacaa 
      taaaagtgt acaggaaat acaccgttt gtgcttcgc 
      tttttcggt gtaattttg tgtttttaa aaacatttc 
      acttgtcac acccactgt gcatttccg ccgataaga 
      aaaactgtg ggaaatggt taaaacgag tgaccagaa 
      atgtgtgaa agtggtggt tttggggga ctttgggcg 
      gagccccag gatgcagta ccactgcat gtccctgtc 
      agaaatggg gcaccgcgt ggacgtgat tgcttacgg 
      aggaacgga gctccttga ctccatcaa agcagaccc 
      cttcatttt ctaccacta tctgctccc agtgcccca 
      acttcccat caagctact caattacgc attcaagac 
      agttatgca agcgattaa tttactatt tctgcttct 
      tatcacgac cagatcaga tattttaat tatgcaaaa 
      cccgcctgc aattcccgc agtggtaat ctgctggtt 
      gtacacacg aattgtaaa tgcgaaatt tgtgataga 
      ttggcataa ctatgctca cactatcat ggccctgag 
      tgtcgggga gcgaaaccc tctggttaa aattacgaa 
      aaaagtgga agttgttgt gggccgcag agctgatca 
      taatttttg cgtcaccaa agcaatgag agacgattt 
      gcaaaaaaa ctggaacat aaaagccac aactttata 
      cgacaggcc gcctttaat atttaaacc acttgagtt 
      ggaagaaaa acacaaatt tttgttaga gtgcggcaa 
      aaagtacaa taaaatatt cttggacgc ccacgaaaa 
      ctcaacggt tttcactga gaaaacaga agagggcat 
      taaattaaa aagcgagag accgggatt gctagtttc 
      cagcactag ttggactga agatgagga tttttccat 
      cttgttaac agccttaca agagtacga aaaacagtg 
      tcatgaagg caatgcaag aaatttgcc taaattgat 
      ttgtgtgat aacaggaaa ggggccgtt aaaagacta 
      ctatcttga caggatttc caaactgac ttgggatta 
      cgttactat aataacccc ctggttaga ttctgaaga 
      ctatccaaa aattttagc ttcagcaga tttgggagt 
      ctctcttca cacaagttc tagtggtct tgaccttcc 
      catgaaagt ggttgatat gtttggttg tggtttacc 
      agtctgtgc ctttgactt taaatgcct aaatgaact 
      agtaaaaga cggagaaaa cagtttcat attcaaatc 
      ccctgaaga gttatcccg gcacgtgtt aaattggtt 
      tgaaaactt cccaagcaa cgagaacca aaaacgcac 
      tgcgtcaaa attcaagac tgaactcca atcattcca 
      aaacctgcg atggaagga aaattggga attggtggc 
      gtcaccagt tttcaaata attagaaaa aaacgttta 
      ttgaaaagt taaactatg aataaattg tacattaaa 
      cgagcaagc gctcgattt cctgctgaa tggcctcaa 
      tttgcggct tcagctgcg acccctcat ttatagtaa 
      cactgcaag caatgaccg accttgaga cgccacaag 
      cgcgcccca gtgcctgtt tcgagcgga cgaaaacat 
      acggcacat tcttgtctt cgcaaagca gtggcaaat 
      gcaggagaa tctccaaag gttcggcgt cagccgcca 
      tcacaataa aagctgcaa taccgcggt tcagggtct 
      tggtgactt cgttagccc ccttgcgga gctgcttgt 
      agttcatgg cctgttgca cgagattca gaattttgg 
      tcgtgagga tggcctcgg ccaaagggt aggctttgg 
      ggttcactt cctccac