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Gene information
General information
TC003519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6221932..6223836
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003519
    iBeetle screen iB_03569

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003519 RA
      MNNNKHDTN VEEIKHIFL GLNREFVKS GLSTKELEN 
      ACLPQCSKH TNSVLFIVF LLLALLLAT SYQYGLFTS 
      AISYFLGVR CLVPNNYFI WEATRPVSN CNFCKNVHK 
      PLILNNVTR EEFAPYAYS SKPIVIKQA FLHWPAMKY 
      FDFNFFKEL YNSISDSYK SVDEECQFL HFKSNFISI 
      RDVFEMSEA RVQNKPGEK SWYVGWGNC HPQILAEMR 
      KYYPKPHFL PEDCEIPSK EYVFMGYDD GATMHLDFI 
      NRLMWQAQL KGSKTWHLV PPPECEDVC SQFSFLVEP 
      GDAVLVDTR VWYHGTQIT PGQFSLSVQ SEYG
    CDS sequence
    • >TC003519 RA
      atgaataac aataaacac gacacaaat gtcgaagaa 
      attaagcac atattcctc ggcttgaac agagaattc 
      gtaaagtct ggcctcagt acaaaagaa ctcgaaaat 
      gcttgcctg ccccaatgt tcaaaacac acaaattca 
      gttttattt atagtattt ttactgcta gcgttgttg 
      ctggcgact tcgtaccaa tatggacta tttacaagt 
      gccattagt tatttccta ggagtgcgg tgtctagtc 
      ccaaacaat tatttcatt tgggaagcc acaagacca 
      gtgtccaac tgcaacttt tgtaaaaat gtccacaag 
      cctcttata ctgaacaac gtgacgcgg gaagaattc 
      gcgccctac gcttacagt tcaaaaccc atcgtcata 
      aaacaggcc tttcttcac tggccagca atgaaatac 
      ttcgatttc aacttcttc aaagaactt tataacagc 
      atatcagac tcgtataag agtgtagac gaggaatgc 
      caatttctc cacttcaag tcaaacttt atatcaatt 
      cgtgatgtc tttgagatg agcgaagca agggtccag 
      aataaacca ggcgaaaaa tcttggtat gtgggatgg 
      ggaaactgc catccacaa atattagct gaaatgagg 
      aagtactac cccaaacct cactttcta ccagaagat 
      tgtgaaatc ccttctaaa gaatacgtt ttcatggga 
      tatgatgat ggtgccaca atgcacctt gattttatt 
      aatagactg atgtggcaa gcgcagcta aagggatca 
      aaaacctgg catctggtg ccaccacct gaatgtgaa 
      gatgtttgc tcacaattt tcatttttg gttgaacct 
      ggagatgca gtcttggtg gacacaagg gtttggtat 
      catggaaca caaatcact ccaggacag ttcagcctt 
      tcagttcag tcagaatat gggtag
    mRNA sequence
    • >TC003519 RA
      agtgtcaag taacacatg tcatgtcaa attgacgtt 
      tgttgtaaa aacactaca gcaaaatgt agttttcca 
      caatgaata acaataaac acgacacaa atgtcgaag 
      aaattaagc acatattcc tcggcttga acagagaat 
      tcgtaaagt ctggcctca gtacaaaag aactcgaaa 
      atgcttgcc tgccccaat gttcaaaac acacaaatt 
      cagttttat ttatagtat ttttactgc tagcgttgt 
      tgctggcga cttcgtacc aatatggac tatttacaa 
      gtgccatta gttatttcc taggagtgc ggtgtctag 
      tcccaaaca attatttca tttgggaag ccacaagac 
      cagtgtcca actgcaact tttgtaaaa atgtccaca 
      agcctctta tactgaaca acgtgacgc gggaagaat 
      tcgcgccct acgcttaca gttcaaaac ccatcgtca 
      taaaacagg cctttcttc actggccag caatgaaat 
      acttcgatt tcaacttct tcaaagaac tttataaca 
      gcatatcag actcgtata agagtgtag acgaggaat 
      gccaatttc tccacttca agtcaaact ttatatcaa 
      ttcgtgatg tctttgaga tgagcgaag caagggtcc 
      agaataaac caggcgaaa aatcttggt atgtgggat 
      ggggaaact gccatccac aaatattag ctgaaatga 
      ggaagtact accccaaac ctcactttc taccagaag 
      attgtgaaa tcccttcta aagaatacg ttttcatgg 
      gatatgatg atggtgcca caatgcacc ttgatttta 
      ttaatagac tgatgtggc aagcgcagc taaagggat 
      caaaaacct ggcatctgg tgccaccac ctgaatgtg 
      aagatgttt gctcacaat tttcatttt tggttgaac 
      ctggagatg cagtcttgg tggacacaa gggtttggt 
      atcatggaa cacaaatca ctccaggac agttcagcc 
      tttcagttc agtcagaat atgggtagt tcccctgca 
      ctggcagct aaaacgcaa attttcgaa aagtctgat 
      ctgcagtgc caatttagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY