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Gene information
General information
TC003518 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6207472..6220748
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003518
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03568

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003518 RA
      MNKVLPGLY VGNYRDSKD ANQLAKHNI THILAIHDS 
      AKRIHSDKH YLCVMASDS PDQNLTQYF SICNDFIHA 
      ARLREGNVL IHCLAGMSR SVTVAVAYI MSVTNLNWK 
      EALKVVRAG RAVANPNLG FQKQLQDFE ATRLAKERR 
      RLKERYPSL ALVNIDREQ CSLMLNNYE ELLQSRSIC 
      EGRCALGEE CPTGICRSK NQNPAAHQP RSRRQSRST 
      RSRSEMCRS PSGTSAAST STRPRSGPA GLRSYTGSA 
      PPSRTSSRV DISSIGVSG NLTAPTTPQ TSPPISPKR 
      RGTVAVPLA LKEDDFDL
    • >TC003518 RB
      MNKVLPGLY VGNYRDSKD ANQLAKHNI THILAIHDS 
      AKRIHSDKH YLCVMASDS PDQNLTQYF SICNDFIHA 
      ARLREGNVL IHCLAGMSR SVTVAVAYI MSVTNLNWK 
      EALKVVRAG RAVANPNLG FQKQLQDFE ATRLAKERR 
      RLKERYPSL ALVNIDREQ CSLMLNNYE ELLQSRSIC 
      EGRCALGEE CPTGICRSK NQNPAAHQP RSRRQSRST 
      RSRSEMCRS PSGTSAAST STRPRSGPA GLRSYTGAL 
      YIYVVMVQL RHLGPVPA
    • >TC003518 RC
      MGNGMNKVL PGLYVGNYR DSKDANQLA KHNITHILA 
      IHDSAKRIH SDKHYLCVM ASDSPDQNL TQYFSICND 
      FIHAARLRE GNVLIHCLA GMSRSVTVA VAYIMSVTN 
      LNWKEALKV VRAGRAVAN PNLGFQKQL QDFEATRLA 
      KERRRLKER YPSLALVNI DREQCSLML NNYEELLQS 
      RSICEGRCA LGEECPTGI CRSKNQNPA AHQPRSRRQ 
      SRSTRSRSE MCRSPSGTS AASTSTRPR SGPAGLRSY 
      TGSAPPSRT SSRVDISSI GVSGNLTAP TTPQTSPPI 
      SPKRRGTVA VPLALKEDD FDL
    CDS sequence
    • >TC003518 RA
      atgaataag gtgttgcct ggcctttat gtgggaaat 
      tacagggac tcaaaagac gctaaccaa ctagccaag 
      cacaacata acacacatc cttgccatc catgactct 
      gcgaaaaga atacattcc gataagcac tatctttgc 
      gtgatggcg tcggattct ccggatcaa aacctgact 
      caatatttt tccatatgc aacgatttc atccacgct 
      gcgaggctc cgagaaggc aacgtttta atacattgt 
      ctggctgga atgtctcgc agtgtgaca gtggcagtg 
      gcgtacatc atgtctgta actaatctc aactggaag 
      gaagcactt aaagtcgtt agagctggt cgcgcggta 
      gccaacccc aacctgggc ttccaaaag caattacag 
      gattttgaa gccacgcgg ttagcaaag gagcgccgc 
      cgcctcaag gagcgctac ccaagttta gctttagta 
      aacatagac agagaacag tgttctttg atgctcaat 
      aactacgag gagttgctg caatctcgg tcaatttgc 
      gaaggtcga tgcgcgttg ggcgaagaa tgtccaacg 
      ggaatttgc agatcgaag aatcaaaac ccagctgct 
      caccagccg cgttcacgg agacagagc agaagcaca 
      agatcgcgt tcggagatg tgtcgaagt cctagtggc 
      actagcgcc gctagcacc agcaccaga ccgcgctcg 
      gggcccgca ggacttcgc agctataca ggttcagct 
      ccgccatct cggaccagt tcccgcgta gatatttct 
      tccattggt gtttccgga aaccttaca gctccaaca 
      acaccgcaa acaagccct ccaatttca ccgaaacgg 
      cgaggaacg gtcgcagtg cctctggct ttgaaagaa 
      gacgatttt gatttatag
    • >TC003518 RB
      atgaataag gtgttgcct ggcctttat gtgggaaat 
      tacagggac tcaaaagac gctaaccaa ctagccaag 
      cacaacata acacacatc cttgccatc catgactct 
      gcgaaaaga atacattcc gataagcac tatctttgc 
      gtgatggcg tcggattct ccggatcaa aacctgact 
      caatatttt tccatatgc aacgatttc atccacgct 
      gcgaggctc cgagaaggc aacgtttta atacattgt 
      ctggctgga atgtctcgc agtgtgaca gtggcagtg 
      gcgtacatc atgtctgta actaatctc aactggaag 
      gaagcactt aaagtcgtt agagctggt cgcgcggta 
      gccaacccc aacctgggc ttccaaaag caattacag 
      gattttgaa gccacgcgg ttagcaaag gagcgccgc 
      cgcctcaag gagcgctac ccaagttta gctttagta 
      aacatagac agagaacag tgttctttg atgctcaat 
      aactacgag gagttgctg caatctcgg tcaatttgc 
      gaaggtcga tgcgcgttg ggcgaagaa tgtccaacg 
      ggaatttgc agatcgaag aatcaaaac ccagctgct 
      caccagccg cgttcacgg agacagagc agaagcaca 
      agatcgcgt tcggagatg tgtcgaagt cctagtggc 
      actagcgcc gctagcacc agcaccaga ccgcgctcg 
      gggcccgca ggacttcgc agctataca ggtgcacta 
      tacatatat gttgtaatg gttcagctc cgccatctc 
      ggaccagtt cccgcgtag
    • >TC003518 RC
      atggggaat ggcatgaat aaggtgttg cctggcctt 
      tatgtggga aattacagg gactcaaaa gacgctaac 
      caactagcc aagcacaac ataacacac atccttgcc 
      atccatgac tctgcgaaa agaatacat tccgataag 
      cactatctt tgcgtgatg gcgtcggat tctccggat 
      caaaacctg actcaatat ttttccata tgcaacgat 
      ttcatccac gctgcgagg ctccgagaa ggcaacgtt 
      ttaatacat tgtctggct ggaatgtct cgcagtgtg 
      acagtggca gtggcgtac atcatgtct gtaactaat 
      ctcaactgg aaggaagca cttaaagtc gttagagct 
      ggtcgcgcg gtagccaac cccaacctg ggcttccaa 
      aagcaatta caggatttt gaagccacg cggttagca 
      aaggagcgc cgccgcctc aaggagcgc tacccaagt 
      ttagcttta gtaaacata gacagagaa cagtgttct 
      ttgatgctc aataactac gaggagttg ctgcaatct 
      cggtcaatt tgcgaaggt cgatgcgcg ttgggcgaa 
      gaatgtcca acgggaatt tgcagatcg aagaatcaa 
      aacccagct gctcaccag ccgcgttca cggagacag 
      agcagaagc acaagatcg cgttcggag atgtgtcga 
      agtcctagt ggcactagc gccgctagc accagcacc 
      agaccgcgc tcggggccc gcaggactt cgcagctat 
      acaggttca gctccgcca tctcggacc agttcccgc 
      gtagatatt tcttccatt ggtgtttcc ggaaacctt 
      acagctcca acaacaccg caaacaagc cctccaatt 
      tcaccgaaa cggcgagga acggtcgca gtgcctctg 
      gctttgaaa gaagacgat tttgattta tag
    mRNA sequence
    • >TC003518 RA
      cgggggcag acagagcgc cgccgccgt ttcagacga 
      tgggatgcg agccgtttc cagttgagc cgtgtttag 
      acatgtgat cgctgcccg agccagcaa ctgccgccc 
      cggagacat ggggaatgg catgaataa ggtgttgcc 
      tggccttta tgtgggaaa ttacaggga ctcaaaaga 
      cgctaacca actagccaa gcacaacat aacacacat 
      ccttgccat ccatgactc tgcgaaaag aatacattc 
      cgataagca ctatctttg cgtgatggc gtcggattc 
      tccggatca aaacctgac tcaatattt ttccatatg 
      caacgattt catccacgc tgcgaggct ccgagaagg 
      caacgtttt aatacattg tctggctgg aatgtctcg 
      cagtgtgac agtggcagt ggcgtacat catgtctgt 
      aactaatct caactggaa ggaagcact taaagtcgt 
      tagagctgg tcgcgcggt agccaaccc caacctggg 
      cttccaaaa gcaattaca ggattttga agccacgcg 
      gttagcaaa ggagcgccg ccgcctcaa ggagcgcta 
      cccaagttt agctttagt aaacataga cagagaaca 
      gtgttcttt gatgctcaa taactacga ggagttgct 
      gcaatctcg gtcaatttg cgaaggtcg atgcgcgtt 
      gggcgaaga atgtccaac gggaatttg cagatcgaa 
      gaatcaaaa cccagctgc tcaccagcc gcgttcacg 
      gagacagag cagaagcac aagatcgcg ttcggagat 
      gtgtcgaag tcctagtgg cactagcgc cgctagcac 
      cagcaccag accgcgctc ggggcccgc aggacttcg 
      cagctatac aggttcagc tccgccatc tcggaccag 
      ttcccgcgt agatatttc ttccattgg tgtttccgg 
      aaaccttac agctccaac aacaccgca aacaagccc 
      tccaatttc accgaaacg gcgaggaac ggtcgcagt 
      gcctctggc tttgaaaga agacgattt tgatttata 
      gccctcgcc acaagaggt caaactctt caaaaattc 
      taggggatg agtaggttt tggtgcgct tggttggag 
      gttaaaagg gtgcaaatt tttgttggt tgtgatttt 
      tgaaagaat ttatataca ccgtggcca ctagaagct 
      tcgtgctta tatattacg aggaaacac tgcttatat 
      tccatccgt gtcgtgttt agatccact ctagggttg 
      tagaaaaat tatgtggta taatgtaac tcatcatat 
      catcaaccc attttgatg actgatttt tttacgaat 
      caaacacct aaacttaca atcattttt caattattc 
      gtgatgctg gggttgaga attcgtcca cttacattt 
      atcacaatc acaatatgt ttctttaaa attttgttt 
      gattgaata cgtctacca tatgattaa gaccttccc 
      atttaaact cgcaatttc tttagtatt tttattttc 
      cattgcata gtttgtcac cgttgttac atgccaaaa 
      agacattat catcgtcaa agatgtttt atatacaca 
      catatattt ataaaataa tctaataaa aaagtaaaa 
      caacacaag taaatcgga gctttttta aacaaacgt 
      tagtacctg tactaaaaa cgactctca tatcagaaa 
      tatgatgtt aaccacgaa ttgtcctcg atttttgtt 
      gaattgggt tcgttaaac tcgaaaaaa atgtgtttc 
      tcgttctga tttgtggca agcgcaaag ttgttttac 
      tgaattaaa aaaaacctt tacatgttt ataatggtg 
      aatatttta atgttaaca agagcaatg acttttccc 
      cagtggaat tagagatag catgctcaa aaccaggct 
      tagtgaaaa taattaagt agcacaaat gaatagtga 
      taaaataca tattttatt agtgaagta ggttgttgt 
      tcatggcgc tggccgccg gatttattg caataaagt 
      acctacaca tttgttatt acggtttat tgtgcagtt 
      taataatgg aacaattgc actgtagct atatttatg 
      tgtatagaa ttgatgata aaaatatgc tacattatt 
      agaaaacca ttcaaaaaa ttaaaaccg gtagcagta 
      aagcaatgc tgagtgtac ctaattgca gttttgtta 
      attggttga cccaaacat tgtttggtg ggtgttata 
      atagtgata tttttttac aagtgacaa aaatgttga 
      tactcgtac tttctacag attttacaa attgtttga 
      agtgaaaat agcaccacg ctgatttct tgttagtta 
      gttactgta aggcagaaa ggcgtaaga tcagtggag 
      gtaatttta tggtctcta aaaacaaca tccttaaaa 
      ttctttcac tgtattatg aagtgatag agggaaaca 
      atcaagaat tgacgacgt aaatggacg ttaaattca 
      agagtacaa ataatttta aaatgtttt tttgtattt 
      atgaggctt tgtactaga ggcatagat gcacacact 
      ttttgtttc tttatactt tattagtca cactttatt 
      gtacaaaat actgtaaaa aatagccac gcaatatac 
      gtagaagtg tccttattt gagttgcta tttttatat 
      ttcaattat tgtacccaa gaatatatt gtcaattca 
      cacctattg attaatcaa caatattga ctcagtggt 
      ccacaaaaa gtgatataa tcataacct gcgttccag 
      tccaatata cattatgtt ttaaataca tttttctag 
      tgtgaaatg acaaaatgc ttggtcact atacataca 
      aatatagaa acgttataa tttacgtat atttacgaa 
      attaataaa taaagaaat tacag
    • >TC003518 RB
      cgggggcag acagagcgc cgccgccgt ttcagacga 
      tgggatgcg agccgtttc cagttgagc cgtgtttag 
      acatgtgat cgctgcccg agccagcaa ctgccgccc 
      cggagacat ggggaatgg catgaataa ggtgttgcc 
      tggccttta tgtgggaaa ttacaggga ctcaaaaga 
      cgctaacca actagccaa gcacaacat aacacacat 
      ccttgccat ccatgactc tgcgaaaag aatacattc 
      cgataagca ctatctttg cgtgatggc gtcggattc 
      tccggatca aaacctgac tcaatattt ttccatatg 
      caacgattt catccacgc tgcgaggct ccgagaagg 
      caacgtttt aatacattg tctggctgg aatgtctcg 
      cagtgtgac agtggcagt ggcgtacat catgtctgt 
      aactaatct caactggaa ggaagcact taaagtcgt 
      tagagctgg tcgcgcggt agccaaccc caacctggg 
      cttccaaaa gcaattaca ggattttga agccacgcg 
      gttagcaaa ggagcgccg ccgcctcaa ggagcgcta 
      cccaagttt agctttagt aaacataga cagagaaca 
      gtgttcttt gatgctcaa taactacga ggagttgct 
      gcaatctcg gtcaatttg cgaaggtcg atgcgcgtt 
      gggcgaaga atgtccaac gggaatttg cagatcgaa 
      gaatcaaaa cccagctgc tcaccagcc gcgttcacg 
      gagacagag cagaagcac aagatcgcg ttcggagat 
      gtgtcgaag tcctagtgg cactagcgc cgctagcac 
      cagcaccag accgcgctc ggggcccgc aggacttcg 
      cagctatac aggtgcact atacatata tgttgtaat 
      ggttcagct ccgccatct cggaccagt tcccgcgta 
      gatatttct tccattggt gtttccgga aaccttaca 
      gctccaaca acaccgcaa acaagccct ccaatttca 
      ccgaaacgg cgaggaacg gtcgcagtg cctctggct 
      ttgaaagaa gacgatttt gatttatag ccctcgcca 
      caagaggtc aaactcttc aaaaattct aggggatga 
      gtaggtttt ggtgcgctt ggttggagg ttaaaaggg 
      tgcaaattt ttgttggtt gtgattttt gaaagaatt 
      tatatacac cgtggccac tagaagctt cgtgcttat 
      atattacga ggaaacact gcttatatt ccatccgtg 
      tcgtgttta gatccactc tagggttgt agaaaaatt 
      atgtggtat aatgtaact catcatatc atcaaccca 
      ttttgatga ctgattttt ttacgaatc aaacaccta 
      aacttacaa tcatttttc aattattcg tgatgctgg 
      ggttgagaa ttcgtccac ttacattta tcacaatca 
      caatatgtt tctttaaaa ttttgtttg attgaatac 
      gtctaccat atgattaag accttccca tttaaactc 
      gcaatttct ttagtattt ttattttcc attgcatag 
      tttgtcacc gttgttaca tgccaaaaa gacattatc 
      atcgtcaaa gatgtttta tatacacac atatattta 
      taaaataat ctaataaaa aagtaaaac aacacaagt 
      aaatcggag cttttttaa acaaacgtt agtacctgt 
      actaaaaac gactctcat atcagaaat atgatgtta 
      accacgaat tgtcctcga tttttgttg aattgggtt 
      cgttaaact cgaaaaaaa tgtgtttct cgttctgat 
      ttgtggcaa gcgcaaagt tgttttact gaattaaaa 
      aaaaccttt acatgttta taatggtga atattttaa 
      tgttaacaa gagcaatga cttttcccc agtggaatt 
      agagatagc atgctcaaa accaggctt agtgaaaat 
      aattaagta gcacaaatg aatagtgat aaaatacat 
      attttatta gtgaagtag gttgttgtt catggcgct 
      ggccgccgg atttattgc aataaagta cctacacat 
      ttgttatta cggtttatt gtgcagttt aataatgga 
      acaattgca ctgtagcta tatttatgt gtatagaat 
      tgatgataa aaatatgct acattatta gaaaaccat 
      tcaaaaaat taaaaccgg tagcagtaa agcaatgct 
      gagtgtacc taattgcag ttttgttaa ttggttgac 
      ccaaacatt gtttggtgg gtgttataa tagtgatat 
      ttttttaca agtgacaaa aatgttgat actcgtact 
      ttctacaga ttttacaaa ttgtttgaa gtgaaaata 
      gcaccacgc tgatttctt gttagttag ttactgtaa 
      ggcagaaag gcgtaagat cagtggagg taattttat 
      ggtctctaa aaacaacat ccttaaaat tctttcact 
      gtattatga agtgataga gggaaacaa tcaagaatt 
      gacgacgta aatggacgt taaattcaa gagtacaaa 
      taattttaa aatgttttt ttgtattta tgaggcttt 
      gtactagag gcatagatg cacacactt tttgtttct 
      ttatacttt attagtcac actttattg tacaaaata 
      ctgtaaaaa atagccacg caatatacg tagaagtgt 
      ccttatttg agttgctat ttttatatt tcaattatt 
      gtacccaag aatatattg tcaattcac acctattga 
      ttaatcaac aatattgac tcagtggtc cacaaaaag 
      tgatataat cataacctg cgttccagt ccaatatac 
      attatgttt taaatacat ttttctagt gtgaaatga 
      caaaatgct tggtcacta tacatacaa atatagaaa 
      cgttataat ttacgtata tttacgaaa ttaataaat 
      aaagaaatt acag
    • >TC003518 RC
      cgggggcag acagagcgc cgccgccgt ttcagacga 
      tgggatgcg agccgtttc cagttgagc cgtgtttag 
      acatgtgat cgctgcccg agccagcaa ctgccgccc 
      cggagacat ggggaatgg catgaataa ggtgttgcc 
      tggccttta tgtgggaaa ttacaggga ctcaaaaga 
      cgctaacca actagccaa gcacaacat aacacacat 
      ccttgccat ccatgactc tgcgaaaag aatacattc 
      cgataagca ctatctttg cgtgatggc gtcggattc 
      tccggatca aaacctgac tcaatattt ttccatatg 
      caacgattt catccacgc tgcgaggct ccgagaagg 
      caacgtttt aatacattg tctggctgg aatgtctcg 
      cagtgtgac agtggcagt ggcgtacat catgtctgt 
      aactaatct caactggaa ggaagcact taaagtcgt 
      tagagctgg tcgcgcggt agccaaccc caacctggg 
      cttccaaaa gcaattaca ggattttga agccacgcg 
      gttagcaaa ggagcgccg ccgcctcaa ggagcgcta 
      cccaagttt agctttagt aaacataga cagagaaca 
      gtgttcttt gatgctcaa taactacga ggagttgct 
      gcaatctcg gtcaatttg cgaaggtcg atgcgcgtt 
      gggcgaaga atgtccaac gggaatttg cagatcgaa 
      gaatcaaaa cccagctgc tcaccagcc gcgttcacg 
      gagacagag cagaagcac aagatcgcg ttcggagat 
      gtgtcgaag tcctagtgg cactagcgc cgctagcac 
      cagcaccag accgcgctc ggggcccgc aggacttcg 
      cagctatac aggttcagc tccgccatc tcggaccag 
      ttcccgcgt agatatttc ttccattgg tgtttccgg 
      aaaccttac agctccaac aacaccgca aacaagccc 
      tccaatttc accgaaacg gcgaggaac ggtcgcagt 
      gcctctggc tttgaaaga agacgattt tgatttata 
      gccctcgcc acaagaggt caaactctt caaaaattc 
      taggggatg agtaggttt tggtgcgct tggttggag 
      gttaaaagg gtgcaaatt tttgttggt tgtgatttt 
      tgaaagaat ttatataca ccgtggcca ctagaagct 
      tcgtgctta tatattacg aggaaacac tgcttatat 
      tccatccgt gtcgtgttt agatccact ctagggttg 
      tagaaaaat tatgtggta taatgtaac tcatcatat 
      catcaaccc attttgatg actgatttt tttacgaat 
      caaacacct aaacttaca atcattttt caattattc 
      gtgatgctg gggttgaga attcgtcca cttacattt 
      atcacaatc acaatatgt ttctttaaa attttgttt 
      gattgaata cgtctacca tatgattaa gaccttccc 
      atttaaact cgcaatttc tttagtatt tttattttc 
      cattgcata gtttgtcac cgttgttac atgccaaaa 
      agacattat catcgtcaa agatgtttt atatacaca 
      catatattt ataaaataa tctaataaa aaagtaaaa 
      caacacaag taaatcgga gctttttta aacaaacgt 
      tagtacctg tactaaaaa cgactctca tatcagaaa 
      tatgatgtt aaccacgaa ttgtcctcg atttttgtt 
      gaattgggt tcgttaaac tcgaaaaaa atgtgtttc 
      tcgttctga tttgtggca agcgcaaag ttgttttac 
      tgaattaaa aaaaacctt tacatgttt ataatggtg 
      aatatttta atgttaaca agagcaatg acttttccc 
      cagtggaat tagagatag catgctcaa aaccaggct 
      tagtgaaaa taattaagt agcacaaat gaatagtga 
      taaaataca tattttatt agtgaagta ggttgttgt 
      tcatggcgc tggccgccg gatttattg caataaagt 
      acctacaca tttgttatt acggtttat tgtgcagtt 
      taataatgg aacaattgc actgtagct atatttatg 
      tgtatagaa ttgatgata aaaatatgc tacattatt 
      agaaaacca ttcaaaaaa ttaaaaccg gtagcagta 
      aagcaatgc tgagtgtac ctaattgca gttttgtta 
      attggttga cccaaacat tgtttggtg ggtgttata 
      atagtgata tttttttac aagtgacaa aaatgttga 
      tactcgtac tttctacag attttacaa attgtttga 
      agtgaaaat agcaccacg ctgatttct tgttagtta 
      gttactgta aggcagaaa ggcgtaaga tcagtggag 
      gtaatttta tggtctcta aaaacaaca tccttaaaa 
      ttctttcac tgtattatg aagtgatag agggaaaca 
      atcaagaat tgacgacgt aaatggacg ttaaattca 
      agagtacaa ataatttta aaatgtttt tttgtattt 
      atgaggctt tgtactaga ggcatagat gcacacact 
      ttttgtttc tttatactt tattagtca cactttatt 
      gtacaaaat actgtaaaa aatagccac gcaatatac 
      gtagaagtg tccttattt gagttgcta tttttatat 
      ttcaattat tgtacccaa gaatatatt gtcaattca 
      cacctattg attaatcaa caatattga ctcagtggt 
      ccacaaaaa gtgatataa tcataacct gcgttccag 
      tccaatata cattatgtt ttaaataca tttttctag 
      tgtgaaatg acaaaatgc ttggtcact atacataca 
      aatatagaa acgttataa tttacgtat atttacgaa 
      attaataaa taaagaaat tacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY