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Gene information
General information
TC003504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6064220..6066547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003504
    iBeetle screen iB_03567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003504 RA
      MNLINNALS AKNVRIHGM SSEKWTTRD KITQYKGLV 
      NLYRRDRQI TEVDTAVAS KKQYKGLQA LRKSIETDR 
      HKLDNAIGG DRQKLRNTL AEHRELQLA HQNSDPKKV 
      IENVHQINF TKRKVLDKL QYQMKLKSQ QLIDLKLEE 
      ALLQDRLKY EVYDKIKEE EVAQIITGK VQDALLKKE 
      AALAIQQTY AEIINVMKK DALYFDGIL LAIQTDYWY 
      QCRCVLNAA KQGQLATEY KNDREQEFE ALEKAVIKD 
      MKTQKVDLE IVREQAENL HSNLKHLLR RDSDLTTCI 
      LKLEESQDF TELRKDLSK IETALTYLR DTTLVSSFE 
      QIYPSLQEQ LRQKRRLNA LAKKCERNR DIILNKMNH 
      AELMNNMLK NTMVETTGE YKKRKKELM DAVEEQNQS 
      KKQCDQLRD RKRDLIAKV RISLRQIQQ LCLPVKTAE 
      ERQHIKKHD FEANADDPP EPPQEDEID GRKIISDLT 
      PKLSYLMAN VSEKMDDSK TDAAYRFYE SLMKERCVE 
      EPPEITTDE SLMGEFSAE VSHLLTRED IKKQSAEIV 
      AAHNKEEDL VPIFQVKRK KRKFK
    CDS sequence
    • >TC003504 RA
      atgaattta ataaataac gccctatcg gctaagaat 
      gtgagaatt cacggcatg tcgtccgaa aaatggaca 
      acaagagac aaaataacg caatacaag ggattggtc 
      aatttgtac agaagagat cgccaaatt acggaagtc 
      gataccgct gtagccagt aaaaaacag tataaaggt 
      ctccaggcg ttacgtaaa tccatcgaa actgataga 
      cataagttg gacaatgca ataggcggt gataggcaa 
      aagctacgc aatactttg gctgaacat cgagaactt 
      caattagcg catcaaaat tccgaccca aaaaaagtc 
      atagaaaac gtccaccag attaatttt acgaagcgc 
      aaagtttta gacaaattg caataccag atgaagcta 
      aaatcgcag caattgatc gatttgaaa ttagaggaa 
      gctctgctc caagaccgc ctgaaatac gaagtttac 
      gataagata aaagaggag gaagtcgcg caaatcatc 
      actgggaaa gtgcaagac gcgctattg aaaaaagaa 
      gcggctttg gcaatacaa caaacttac gccgaaata 
      attaacgtt atgaaaaaa gatgcgctt tattttgac 
      ggaatttta ttagccatt caaacggac tattggtat 
      caatgtagg tgtgtcttg aacgccgcc aaacagggc 
      caattagcc actgaatat aagaacgac agagagcaa 
      gaatttgaa gccttggaa aaggcggtt atcaaagac 
      atgaaaaca caaaaagtg gacttggaa atagtccgc 
      gaacaagcc gaaaattta cattcaaat ttaaagcat 
      ttattgagg agagacagc gacctaacg acttgtata 
      ttgaaactt gaagaatca caagacttt acggagtta 
      cgtaaagat ttgagtaaa attgaaact gcgttaaca 
      tacttgaga gacacgaca ctggtctct tctttcgaa 
      cagatttat ccaagtttg caagaacaa ttgagacag 
      aagaggcga ttaaatgcg cttgcaaaa aaatgtgaa 
      cgtaataga gacataata ttgaataaa atgaaccat 
      gctgagctc atgaacaat atgttgaaa aatacaatg 
      gtggagaca accggagaa tataaaaaa cgtaagaaa 
      gaattaatg gatgccgtt gaggagcaa aaccaaagc 
      aaaaaacag tgtgaccag ctacgagat cgcaaacgt 
      gatttgata gcaaaagtg agaatttca ctcagacaa 
      atccaacag ttgtgtttg cccgtaaaa acggccgag 
      gagcgtcag catattaaa aaacacgat ttcgaagcc 
      aatgctgat gaccctcca gagccccca caagaagat 
      gaaattgat gggcgtaaa ataatatca gatttgacc 
      ccaaaattg agttatttg atggcaaac gtgtctgag 
      aagatggac gattctaag accgacgcc gcttacaga 
      ttttatgaa agtttgatg aaagagcgt tgcgttgaa 
      gagccgcct gaaatcaca actgacgaa tctcttatg 
      ggggagttc tctgctgaa gtgtcccat cttctcact 
      agagaggat attaagaag caaagtgct gagatcgtc 
      gctgcccat aacaaggag gaagatctg gtgccaata 
      tttcaagtt aaaagaaaa aagaggaag ttcaaatag
    mRNA sequence
    • >TC003504 RA
      ttctggtag atgaagtaa tttcaatca aattggcta 
      atttatttg ataaaaatt cagttattt aatttgcga 
      tttttaata atcaagtgg tttcccgta taatgaagt 
      tttttttta agacaggag tttaaatag ttaatttgt 
      agtttttca aaatgaatt taataaata acgccctat 
      cggctaaga atgtgagaa ttcacggca tgtcgtccg 
      aaaaatgga caacaagag acaaaataa cgcaataca 
      agggattgg tcaatttgt acagaagag atcgccaaa 
      ttacggaag tcgataccg ctgtagcca gtaaaaaac 
      agtataaag gtctccagg cgttacgta aatccatcg 
      aaactgata gacataagt tggacaatg caataggcg 
      gtgataggc aaaagctac gcaatactt tggctgaac 
      atcgagaac ttcaattag cgcatcaaa attccgacc 
      caaaaaaag tcatagaaa acgtccacc agattaatt 
      ttacgaagc gcaaagttt tagacaaat tgcaatacc 
      agatgaagc taaaatcgc agcaattga tcgatttga 
      aattagagg aagctctgc tccaagacc gcctgaaat 
      acgaagttt acgataaga taaaagagg aggaagtcg 
      cgcaaatca tcactggga aagtgcaag acgcgctat 
      tgaaaaaag aagcggctt tggcaatac aacaaactt 
      acgccgaaa taattaacg ttatgaaaa aagatgcgc 
      tttattttg acggaattt tattagcca ttcaaacgg 
      actattggt atcaatgta ggtgtgtct tgaacgccg 
      ccaaacagg gccaattag ccactgaat ataagaacg 
      acagagagc aagaatttg aagccttgg aaaaggcgg 
      ttatcaaag acatgaaaa cacaaaaag tggacttgg 
      aaatagtcc gcgaacaag ccgaaaatt tacattcaa 
      atttaaagc atttattga ggagagaca gcgacctaa 
      cgacttgta tattgaaac ttgaagaat cacaagact 
      ttacggagt tacgtaaag atttgagta aaattgaaa 
      ctgcgttaa catacttga gagacacga cactggtct 
      cttctttcg aacagattt atccaagtt tgcaagaac 
      aattgagac agaagaggc gattaaatg cgcttgcaa 
      aaaaatgtg aacgtaata gagacataa tattgaata 
      aaatgaacc atgctgagc tcatgaaca atatgttga 
      aaaatacaa tggtggaga caaccggag aatataaaa 
      aacgtaaga aagaattaa tggatgccg ttgaggagc 
      aaaaccaaa gcaaaaaac agtgtgacc agctacgag 
      atcgcaaac gtgatttga tagcaaaag tgagaattt 
      cactcagac aaatccaac agttgtgtt tgcccgtaa 
      aaacggccg aggagcgtc agcatatta aaaaacacg 
      atttcgaag ccaatgctg atgaccctc cagagcccc 
      cacaagaag atgaaattg atgggcgta aaataatat 
      cagatttga ccccaaaat tgagttatt tgatggcaa 
      acgtgtctg agaagatgg acgattcta agaccgacg 
      ccgcttaca gattttatg aaagtttga tgaaagagc 
      gttgcgttg aagagccgc ctgaaatca caactgacg 
      aatctctta tgggggagt tctctgctg aagtgtccc 
      atcttctca ctagagagg atattaaga agcaaagtg 
      ctgagatcg tcgctgccc ataacaagg aggaagatc 
      tggtgccaa tatttcaag ttaaaagaa aaaagagga 
      agttcaaat agagtctgg cacctgctc tagctacta 
      tctatatta tttttatta aatattgtg aaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY