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Gene information
General information
TC003480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1256447..1258592
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003480
    iBeetle screen iB_03563
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment distal not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment:  The upper joint of the trochanter, normally easily seen due to a strong fluorescence, could not be found.
    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs tibiotarsus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs trochanter not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003480 RA
      MSIIASWLR YNKFHTQLI NIKYSPVNK SFKNLPTLK 
      SYPVIGHSY LFFPRGKYK SERLTEAFV DISKTLGPI 
      FKLNLGGSA MVVTLDPDH TRILFQNEG TRPERPPFP 
      ALLHFRRKR FSSVGVVPG NGEEWYKMR KGVTPLLKL 
      QLIEPYKRQ QEDIAKTFV EYVKTHRDE NFVLRDIFS 
      HLLKFTIEA ISIVSPGHR FHCLFSDNL ETEQIIKAS 
      VDFMDGLYG TLIEPPFWK LWKTPSYKK LESSHNTIY 
      KILERHLEQ IKFQFSENP ESVKESQPY MYSLFSNDQ 
      LSWDDKIML AMEIFLGGI DATATTISF TLHYLSQNP 
      EIQKMARSQ NTDFLKACI RETLRLSPT AGGNSRFLS 
      NNTVIGGYL IPKGTLLLS LNSGMARDE RYFKDAQKY 
      RPQRFVRAT REDFHRYAS LPFGHGPRM CPGKRVAEN 
      EIVILLTEI LKNFALESA GSSDVGMVF RMNRIPDKP 
      ISVRFVDTN H
    CDS sequence
    • >TC003480 RA
      atgagtatc atagcaagt tggttgaga tacaataag 
      tttcacacc caattaatc aatataaaa tattctccg 
      gttaataag tcttttaaa aatttgccc actctaaaa 
      tcgtacccc gtaataggc cactcgtat ttgtttttt 
      cctagaggt aaatataag tccgaacgt ttgactgaa 
      gcttttgtc gacatcagt aaaactctg gggccaatt 
      tttaaatta aatttgggt gggtcagca atggttgtc 
      acactcgac ccagaccat acgagaatt ctgtttcaa 
      aatgaaggg acgaggcct gaaaggccc ccctttccg 
      gctttgctc cactttagg cggaaaaga ttcagcagt 
      gtgggagtt gtgcccggg aatggggag gagtggtac 
      aagatgaga aaaggagta acaccttta ttaaaactg 
      caattgata gaaccgtac aaaaggcaa caagaagac 
      attgctaag acttttgtg gaatatgta aaaacgcac 
      agagacgaa aatttcgtt ttacgtgat attttttcg 
      catttgttg aagtttaca atcgaggcg atctcgatt 
      gtgtctcca ggacatcgc ttccactgc cttttcagc 
      gataatttg gagaccgaa caaatcatt aaagccagt 
      gtagacttt atggacggt ttatacggc acattaatc 
      gaacccccg ttttggaaa ttatggaaa acaccatct 
      tataagaaa cttgaatcg tctcacaac acaatctac 
      aagattttg gagcgacat ttggaacag atcaagttt 
      cagttctcc gaaaaccct gaatcagtg aaagagtca 
      caaccctac atgtactcc cttttcagc aacgaccaa 
      ctttcatgg gacgataaa attatgcta gctatggag 
      atttttcta ggtggtatc gatgctaca gctacaact 
      atttccttc acgttgcac tacttatcc caaaatcct 
      gaaattcaa aaaatggct cgctcacaa aatactgat 
      tttctaaaa gcttgtatt agagaaact ctgaggctg 
      tcgcccaca gctggtggc aattccaga tttttaagc 
      aataatacc gtcatcggg ggctacttg atacctaaa 
      gggacgctg cttttatcg cttaactca gggatggca 
      cgtgatgaa agatacttt aaagatgcg caaaagtat 
      cgtcctcaa agatttgtt agagcaacg agggaagat 
      tttcatcgc tatgcctcg ttgcctttt ggtcatggc 
      cccaggatg tgcccaggg aagagagtc gctgaaaat 
      gagatagta attttattg acagagatc ctgaaaaac 
      tttgcctta gaatcggcg ggaagttcg gacgtgggc 
      atggttttc cggatgaac cggattccg gataagccg 
      ataagtgta agatttgta gatacaaat cactaa
    mRNA sequence
    • >TC003480 RA
      tgtgagcag tttttgtta gtaaacaca gtagcgtgt 
      ttgtaagtg tccattgtt atatttgtg tagcttttc 
      ggtctgtga agtgcaaac ataaaattt attaaacga 
      gtcggtagg tcgccggta atacatata ttcaagaac 
      tgtatgtat aaattcaaa tttattcga gactctgat 
      gctcgaacc aactttata ttccggatt ttggttgct 
      tgtaatcac catgagtat catagcaag ttggttgag 
      atacaataa gtttcacac ccaattaat caatataaa 
      atattctcc ggttaataa gtcttttaa aaatttgcc 
      cactctaaa atcgtaccc cgtaatagg ccactcgta 
      tttgttttt tcctagagg taaatataa gtccgaacg 
      tttgactga agcttttgt cgacatcag taaaactct 
      ggggccaat ttttaaatt aaatttggg tgggtcagc 
      aatggttgt cacactcga cccagacca tacgagaat 
      tctgtttca aaatgaagg gacgaggcc tgaaaggcc 
      cccctttcc ggctttgct ccactttag gcggaaaag 
      attcagcag tgtgggagt tgtgcccgg gaatgggga 
      ggagtggta caagatgag aaaaggagt aacaccttt 
      attaaaact gcaattgat agaaccgta caaaaggca 
      acaagaaga cattgctaa gacttttgt ggaatatgt 
      aaaaacgca cagagacga aaatttcgt tttacgtga 
      tattttttc gcatttgtt gaagtttac aatcgaggc 
      gatctcgat tgtgtctcc aggacatcg cttccactg 
      ccttttcag cgataattt ggagaccga acaaatcat 
      taaagccag tgtagactt tatggacgg tttatacgg 
      cacattaat cgaaccccc gttttggaa attatggaa 
      aacaccatc ttataagaa acttgaatc gtctcacaa 
      cacaatcta caagatttt ggagcgaca tttggaaca 
      gatcaagtt tcagttctc cgaaaaccc tgaatcagt 
      gaaagagtc acaacccta catgtactc ccttttcag 
      caacgacca actttcatg ggacgataa aattatgct 
      agctatgga gatttttct aggtggtat cgatgctac 
      agctacaac tatttcctt cacgttgca ctacttatc 
      ccaaaatcc tgaaattca aaaaatggc tcgctcaca 
      aaatactga ttttctaaa agcttgtat tagagaaac 
      tctgaggct gtcgcccac agctggtgg caattccag 
      atttttaag caataatac cgtcatcgg gggctactt 
      gatacctaa agggacgct gcttttatc gcttaactc 
      agggatggc acgtgatga aagatactt taaagatgc 
      gcaaaagta tcgtcctca aagatttgt tagagcaac 
      gagggaaga ttttcatcg ctatgcctc gttgccttt 
      tggtcatgg ccccaggat gtgcccagg gaagagagt 
      cgctgaaaa tgagatagt aattttatt gacagagat 
      cctgaaaaa ctttgcctt agaatcggc gggaagttc 
      ggacgtggg catggtttt ccggatgaa ccggattcc 
      ggataagcc gataagtgt aagatttgt agatacaaa 
      tcactaaat atttattat ttcattgta caacaatac 
      atgttttca aaatta