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Gene information
General information
TC003467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5529840..5531410
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003467
      iBeetle screen iB_03562

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003467 RA
        MLPTLLLIV TAFASNTRA NLRDDCNKL 
        FNPLNYMDQ LPSLALPYV NNVPIQPSP 
        VVPTKVINV VTEYVYRNP VCVKISGKK 
        SLCKSKNPQ ANNIEHLVT KQLFVKDRQ 
        MKAHRGPFS LEDGLGMYL QGSEEPRPF 
        MKQENTPQL SPEEVKDML IEDRLDQLE 
        TILPHYTRR RTYQTTTIT VTKVKSNNR 
        ATATLLVKN CIPQGFELC PPKIKAKRR 
        KSKIAKASN VTFEQNYFG
      CDS sequence
      • >TC003467 RA
        atgctaccg actctattg ctgattgtt accgcgttt 
        gcttcaaac acgcgagct aatttgcgc gatgattgc 
        aacaaatta ttcaatccg ctcaattat atggaccaa 
        ctaccgtct ttggcgctc ccttacgtt aacaacgtc 
        cccatccaa ccatcccca gtggttccc accaaggtg 
        ataaatgtg gtgacggaa tacgtgtac cggaacccc 
        gtgtgtgtg aaaatcagt ggcaagaag tccttgtgc 
        aagtccaaa aatccgcaa gccaacaac attgagcat 
        ttggtcacg aagcagtta tttgtgaag gacagacaa 
        atgaaggca catcggggg ccgttcagt ctggaggac 
        ggcttaggg atgtatttg caaggaagc gaagaaccg 
        aggcccttt atgaagcaa gagaacacg ccgcagtta 
        tcgccggaa gaagttaaa gatatgctg attgaagat 
        aggttggat cagttggag acgattctt ccgcattac 
        acgagacga aggacttat caaaccacg accatcaca 
        gtaacaaaa gtgaaaagt aataatagg gccactgcc 
        acacttctt gtcaaaaac tgtattcct caggggttt 
        gaattgtgt ccgccgaaa attaaggcg aaacggagg 
        aaatcgaaa attgcaaaa gctagcaat gtaacgttc 
        gaacaaaat tatttcggt tga
      mRNA sequence
      • >TC003467 RA
        gtcgagcga cccatccga tcgcaaaaa tgctaccga 
        ctctattgc tgattgtta ccgcgtttg cttcaaaca 
        cgcgagcta atttgcgcg atgattgca acaaattat 
        tcaatccgc tcaattata tggaccaac taccgtctt 
        tggcgctcc cttacgtta acaacgtcc ccatccaac 
        catccccag tggttccca ccaaggtga taaatgtgg 
        tgacggaat acgtgtacc ggaaccccg tgtgtgtga 
        aaatcagtg gcaagaagt ccttgtgca agtccaaaa 
        atccgcaag ccaacaaca ttgagcatt tggtcacga 
        agcagttat ttgtgaagg acagacaaa tgaaggcac 
        atcgggggc cgttcagtc tggaggacg gcttaggga 
        tgtatttgc aaggaagcg aagaaccga ggcccttta 
        tgaagcaag agaacacgc cgcagttat cgccggaag 
        aagttaaag atatgctga ttgaagata ggttggatc 
        agttggaga cgattcttc cgcattaca cgagacgaa 
        ggacttatc aaaccacga ccatcacag taacaaaag 
        tgaaaagta ataataggg ccactgcca cacttcttg 
        tcaaaaact gtattcctc aggggtttg aattgtgtc 
        cgccgaaaa ttaaggcga aacggagga aatcgaaaa 
        ttgcaaaag ctagcaatg taacgttcg aacaaaatt 
        atttcggtt gaacgcttt acacatttt taacaaata 
        tttattaat gcaatttgc aattacaga actgttgtg 
        cttgttctt tacttgaac tgtttaatt gccttcttt 
        aatagtttt ataagagta atatttgtt acatacttt 
        tgtaattta ttgttcaat actctaatt aacacgtcc 
        acaatttag tgctaacta cggtaatct atcctcata 
        atcctgttt taattaaaa aattacgct aatttgaaa 
        tgactttga taaaagcta gtctcaaac ataaaataa 
        tgtgaatta atgttgtcc ttacctttg tgcttttgt 
        aaaaaaaat acgtatttt taggaactt aaataaata 
        aacattaaa ctacattta tggtgacaa aactatcag 
        caagacagc aagttacaa acgtgtata gttataagt 
        gttattgtt ttttttagt agatttgtt tagcttata 
        aagtttata aataattta taggtaata attgtacag 
        aaaaaaatg ttcctttta ggtgccttt acatttaat 
        ttaattatg taattagtt aagttatgt attatcgta 
        tttatggtt acctttagt ttttagtta catttcatc 
        aaagaatta tttggaacc gaaaaacct atactttgt 
        tacaactaa aacctaata aaattgttg tttgacttt 
        agagtacaa aaagctaat tatttttag cagtgtttg 
        tgcttagta atgcaaata atgaacagt tttcttgtt 
        gtgttattg tacttattg taaacgtta cagttagat 
        gcctgtaat ttaatttta ctcaacttt tctgtctga 
        tcgaataaa agccgttag atatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY