| General information | |||
| TC003464 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5511480..5520708 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003464 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03561 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003464 RA
MDLSSCTNW FCAKRNEKV PITSTDCHS VVTSGPGPS PGSSNSCDD VIPPQKNCF RLVVLGSAR VGKTCLVSR FLGGKFQES YTPTIEDFH RKLYRIRGE IYQLDILDT SGNHPFPAM RRLSFLTGD LFIIVFSMD SRETFEEAI RLREQILET KINAGAASN SGGLTRKKT LPRVPMILA GNKSDKEMK TVTAEEAQL YCDTQDSGC AFVETSAKK NLKVDEVFY QLFVVANLP QEMAPNHHK RISANFGAP CPLPPSTPS YHTKKYTLS VKRRLSDAC GVVTPNVRR PSIRTDLMI MRTKTCNVG DNISNPNNS PLSWRRVDS NCSMQ
- >TC003464 RA
atggatcta agctcttgt acgaattgg ttttgtgcc aaacggaac gagaaagtt ccaattaca tcaacagat tgtcattcg gtcgtaaca tcaggacct ggaccaagc cctggaagt tcaaattct tgtgacgac gtgatccct ccacaaaaa aactgtttc agattagta gttctggga tcagctaga gtaggcaaa acttgttta gtttcgcga ttcctgggt ggaaaattt caagagagc tatactccg accattgag gattttcac cggaagttg tatcgaatt agaggggaa atttaccag cttgatatt ctggacact tccggcaac cacccgttt cctgcaatg agaaggcta tcgtttctc acaggggat ttatttatt atcgtgttc agtatggat agccgtgag acgtttgaa gaggcaata agattacga gagcagata ttagaaacg aaaattaat gcaggggcg gcttcaaac agcggcggt ttaacgagg aagaaaacc ttaccgcga gttccgatg atcctagca ggaaataaa agcgataag gaaatgaag acagtgact gcagaggaa gctcaatta tactgtgac actcaagat tcaggctgt gcctttgta gaaacatca gccaagaaa aacttaaaa gtagatgaa gttttttac caattgttt gtggtggct aatctgccg caagaaatg gcacccaac catcacaaa agaatcagt gccaatttt ggggccccg tgtcctctg cctccttct acaccatcc taccataca aagaaatat acgctttcg gtgaaacgg agattaagc gacgcgtgt ggtgtcgtt acgcccaat gtcagacga ccgagtatt cggacagat ttaatgata atgaggacc aaaacgtgc aatgtgggc gataacatt tcgaacccc aataactca cctcttagt tggagacgg gtcgacagc aactgttca atgcaatag
- >TC003464 RA
cgcaggtcg caaatgtgg acgattaat ggtgttttt aatttttta tcagttgaa tattcatag ctttaatac acggactaa aatggaggc cttcaaaaa tttcgtatt caataaaat tatcaaaca ttgctgact attctcatt caaaaatat ctgcatgag tgcatttaa aatctcgtg atgtactgt aacacaatt aagccgaat tatggatct aagctcttg tacgaattg gttttgtgc caaacggaa cgagaaagt tccaattac atcaacaga ttgtcattc ggtcgtaac atcaggacc tggaccaag ccctggaag ttcaaattc ttgtgacga cgtgatccc tccacaaaa aaactgttt cagattagt agttctggg atcagctag agtaggcaa aacttgttt agtttcgcg attcctggg tggaaaatt tcaagagag ctatactcc gaccattga ggattttca ccggaagtt gtatcgaat tagagggga aatttacca gcttgatat tctggacac ttccggcaa ccacccgtt tcctgcaat gagaaggct atcgtttct cacagggga tttatttat tatcgtgtt cagtatgga tagccgtga gacgtttga agaggcaat aagattacg agagcagat attagaaac gaaaattaa tgcaggggc ggcttcaaa cagcggcgg tttaacgag gaagaaaac cttaccgcg agttccgat gatcctagc aggaaataa aagcgataa ggaaatgaa gacagtgac tgcagagga agctcaatt atactgtga cactcaaga ttcaggctg tgcctttgt agaaacatc agccaagaa aaacttaaa agtagatga agtttttta ccaattgtt tgtggtggc taatctgcc gcaagaaat ggcacccaa ccatcacaa aagaatcag tgccaattt tggggcccc gtgtcctct gcctccttc tacaccatc ctaccatac aaagaaata tacgctttc ggtgaaacg gagattaag cgacgcgtg tggtgtcgt tacgcccaa tgtcagacg accgagtat tcggacaga tttaatgat aatgaggac caaaacgtg caatgtggg cgataacat ttcgaaccc caataactc acctcttag ttggagacg ggtcgacag caactgttc aatgcaata gtcaaattt caaattttc aaaacggat actggccag tgaccacac agcaataat tcgacttta tttcatttt aaagtaaaa aaattacaa agaagttat ctaactgta agacctttt cctatttat ttagaaaga cctacagtt gaaaaacca ttttacaaa agtggtcaa ctaactcca ttgtttgag cgaaagata aacttaatt attatgatt taggaaccg ggctctact tattagtaa tttaattca cttaggtac tacacactc agcctaggt gtagatgta gtgtttcac aaaaaatat gtacctagt atttgtgta ggtaatcgt caatagcag taataaacg tgttaagac tgatcaaac atattgcga tacacttgt tgtgaatta ttccgatta ctctagtta attttttta cacctagca gctaaaacc aagaaaaac aaagcaatg caaatttta aaatccttg attatttgg tataaatgg agttcactt ctaacttta tcctctcat acttaccat ttgtctgtg gtttggcat ttgaaaggt tttcgcaaa caacgaatc taattaaaa agggtttac tattcgaaa gcgaaaact aacaaatat tgattcaac acaaaaata tctttactt ttatattga aatgctctc tccatgtgt gtaaccacc tataaactg taagctctt cctttctct acctccttt ctgaccact gaaatccac ttcgtagtt catattgca atgttattt atgtacaat ttgtagatg ttaattatt attattgta gacgcgcga gagtattat ttaaaaagt taaacagaa agcgattac ctactgaat tcataatat gagggatct aacagtacc attgttata atttacaga tcataaaat gtctattga tttatagta cataaatct ataaaatgt gggcgctaa tatataaca tatcttttt tgctcttgt gttgtgata ttgttagca aatcacttt atgtaaagg atgtgaaac ccctagata attgcctaa tttcggaaa ctttagttt cttcaaaga aagtaaatc aaatttttg ataattagt atatgcttt atatttgaa taacagaac taatgttct gccttttat acagggtga gtcaattta tcggtctga aattctgaa atcaaatgg tactaagtg tatttttgg ttgcatcta agaaaattt cacaattga ttcaccctg tactacgat tcagaaaaa aattataat tcagtaatt taaatttag caataaaaa ctgttctat ttcgagaaa agtattgct taattaaag aaaactgtt atagatatt acacgaaat aaagtgctc atttgagta attgattat gaccaattt tattatagt ttatagaca tatgtccaa tacgtacga ttttgtaat ttaattagt ttacgtata tgcatccaa attttacaa aatattttt gattctaca aaacttaga aatactcaa atgtgtaat cgtaactta gttcataaa atgtagtct atggctatg ccacatcag ctattattt atactacat attataatt ttattgtta gaaatggga tcaggaata agaactatt ctcatttta ttttttgta ctagtaaac aagatgtaa agctatgag tatttgagc cgtatttag tgttttttt ttgtttttg tagaaggat tattacatc attaaaagc agcgtattg ttaagcaaa tgtctcaat atttgtttt gatttattg aaacatttt ttacttgga acgggagat aaacgatac gattattat catgtgtct acattctta catgtgaat taaattcat gtaaatcta caatcagga acaaattgt tttataaaa atttgtgtg aagctttaa cgtactcgt atatttaac tgcaatgtt ctatcgtgg ctttaaaaa cagtacttg tctaataat taccaacta agcat
