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Gene information
General information
TC003460 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 835822..851454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003460
    iBeetle screen iB_03560
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: It's not sure whether larvae are still alive. Almost none of them hatched.  
    larval musculature partially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature partially orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 6

    oblique dorsal thoracic muscles size increased - Number of animals showing the phenotype: 6

    Comment: the inner oblique muscles are thicker
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003460 RA
      MFVVRAWTG LLAVLVLRL ETVQGEILT PPYFNLAEG 
      RKITATATC GVDTEGPEL YCKLVGANA ENDLNVNVI 
      QGQFCDVCD PTRPDKMHP PEFAVDGME TWWQSPPLS 
      RGMKYNEVN LTIDLGQEF HVAYVFIRM GNSPRPGLW 
      VLEKSADYG KTYTPWQYF SDSPGDCET YFGKESLQP 
      ITKDDSVIC TTEYSKIVP LEGGEIAIS LLNNRPSAK 
      NYFNSTILQ EWTRATNVR LKLLRTKNF LGHLMSVAR 
      QDPTVTRRY FYSIKDISI GGRCMCNGH ADTCDIQDS 
      QDPSVLLCR CQHNTCGAK CDTCCPGFE QKKWQQSKI 
      NHPFVCEPC NCFQHSNEC VYDEEVDRK GLSLDIHGR 
      YAGGGVCLN CQHNTDGIN CNKCKPTFY RPFGKFEND 
      TDACQPCNC NHFYSTGNC AEGSGQCEC KKQFQPPNC 
      DSCSFGYFG YPNCRPCEC FLNGTKDLQ CEARNGQCP 
      CLYNYGGQF CGECAPGYY SFPDCKHCE CNPEGSVTN 
      ACEQESGNC TCKNNFGGK RCDECQHGY YNYPTCDYC 
      NCDIKGTKE GICDKTTGQ CMCKEGYTG DRCDSCLAG 
      YYGYPDCKP CNCSKVGSV GFSCSATGK CSCSTNYGG 
      KTCDQCNPG YFKYPECLP CECDSHGSI GVSCDGEGK 
      CECHHNFAG LRCDSCREG FYNFPACED CNCHPAGVV 
      AGFAGCGSV PAGELCQCK ERVEGRICN KCRPLYWNL 
      SPNNPQGCE ECKCNISGV LGGIAVCDS ESGECVCKS 
      LVMGRGCTE CVDGTYNLQ EENLFGCTD CDCDVGGSV 
      NNICDKATG QCLCHSRVK GRTCKEPLE THYFPTLYQ 
      YQYEVEDGR TPANTLVRY ANDERTFPG YSWKGYAVF 
      GPLQKQILQ DVIILKPSL YRMVLRFVN KNPETVIGK 
      IRITPDNPS DNEQEIQVQ FRNSTVPAF VTVSGPVGN 
      TPQPFVINP GRWQISIEV TKDLLLDYF VLLPEDFYL 
      ATILNKKVE TPCTIDGPG LCHDYTYLN LTQFSTTYG 
      VGGYVPEGR RSTKLREYF QDNEHLAKI GVGNRVPVL 
      NLAQPNVSF NITVPKPGQ YVIIINYVT PKEDQRTHK 
      IDVTLQSGR HTNDGRIVL YSCQYTTLC RQVITTPEG 
      IVAVQQVDG NEIKVDLHS SNADVGIHS IVAIPYEEW 
      SLDYIKPKS VCIRKDGKC LAASFRYPP ETKKIQFEE 
      NNADLATTL WNNTYMWLD PSYDTLDLK GKVPTPGYY 
      TFIVHYYQP NFQDFDVTV LMQNGQFYE ATLPVHYCP 
      SRSGCRSVI TQVDGNTKF SLTENFMLT LKEPGNKNV 
      YLDYLLVIP TDFYSDRYL EEEDVDRTG EFISTCGSN 
      HFNINTTTE GFCKDSVFS ITAGHNSGA LPCGCDFAG 
      SLSFECDKF GGQCRCKPN IIGRKCEAC KTGYFGFPD 
      CKPCNCPSI AFCEPTTGQ CICPPHVVG ERCDQCEPL 
      TYGYDPIIG CEECKCNIL GIEGTEQCD LNNGSCHCK 
      PNVVGRQCD KCLAGHYAF PYCEFCSCD YRGTTSEIC 
      DQATAECFC KKNVVGPTC EICREGTFN LQEENEHGC 
      TECFCFGKT TRCTSSKLI RTSVLQMDD WELVGLNFS 
      TRLEIYPLN LTVHNAEEN SIGVAFNSV DFKNTTVYF 
      TAPSDYTGK KLTAYGGFL NYTLYYTIT PGEGSAVSE 
      PDVILEGRK TYLTYSSYE QPTAAVEYH ASVQLVEEN 
      FELPTGLPA RRDHIMEVL EDVRGVYIR ATYWTASVT 
      TRLSHVIQD DAISYQYYS GDNRNLAFA LTVEECRCP 
      ENYQGLSCE ECAPGYYRV EGGPHGGYC VPCQCHGHA 
      TECDVNTGI CLNCTHNTR GDHCELCEV GYHGNALAG 
      TPMDCLICA CPLPVGSNN FATACDVSA DGEKISCEC 
      KEGYFGARC QSCAAGFYG RPESLGDFC KPCQCSGNI 
      NPDDPSSCD TVSGECLRC LNNTYGKAC ALCAPGYFG 
      DAVNLKDCQ SCICDELGT DHCNSFTGE CVCKPNVIG 
      EKCDRCEVK HYGFQTGAG CVPCDCAEA SDSSQCDDV 
      TGQCRCKPG VSGRSCNRC SAGFWNYTT DGCVSCGCK 
      SEYSLGFGC NALTGQCEC LQGVVGEKC DQCPHRWVF 
      VPDYGCHQC DSCTHALLD DTDALRHLI DPIIVEFDS 
      ANSGYFTRR KLDNMRDLL NELKPKFDE VDPKQINLD 
      LYIDELQTL EQDSKNLNR KTNYSLENS DSLRVSSID 
      LKERAELLL DDVQNAEDD SFRVIDEIN KITFQLNKD 
      ENPEIDSAL EEGQELLDA IKRYNLTGR EEMADDELD 
      KVSNLLLNV SNFKIPVDY LEEKTDKVK ESLKNFNDK 
      LDDLYNQTQ YSLSKANEA QRIIEKSG
    CDS sequence
    • >TC003460 RA
      atgttcgtg gtacgcgcg tggacgggc cttttggcc 
      gtccttgtg cttcggctt gaaacagtg cagggtgaa 
      attttaacc ccgccgtat ttcaatctg gcagagggt 
      agaaaaatt acagcgact gcgacttgc ggagtcgac 
      accgaaggg cccgagctt tactgcaaa cttgtgggg 
      gctaatgcc gaaaatgat ctaaatgtt aatgtgata 
      caggggcag ttttgtgat gtctgcgac cccacaaga 
      ccggacaaa atgcaccca ccggagttt gctgtggac 
      ggcatggaa acttggtgg caaagtcct ccactatct 
      cgaggaatg aaatataac gaggtcaac ttaaccatt 
      gatctcggc caagaattt cacgtcgct tacgttttt 
      attcgaatg gggaattcc cccaggccc ggcttatgg 
      gtgttggaa aaatcggcc gattacggc aaaacctac 
      acgccttgg caatatttc tcggattct cctggcgat 
      tgtgaaact tactttggc aaagaaagt ctgcagcca 
      atcactaaa gacgattcc gtcatttgt acgactgaa 
      tattcaaaa atagtacca ctagaaggc ggagaaatc 
      gcaatttcg ttgttgaac aaccggcca tcagctaag 
      aattatttc aattctacg attttgcaa gaatggacc 
      cgagccacc aacgtccga ttgaagctt ttaagaacg 
      aaaaacttc ctagggcat ctcatgtcg gtggctaga 
      caagatccg actgttaca cgaagatac ttttattcg 
      attaaagat atcagtatt ggaggccgt tgcatgtgc 
      aatggacac gctgatacc tgcgatatt caagactct 
      caagacccg agcgttttg ttatgtcgc tgtcaacat 
      aatacttgt ggggcgaaa tgtgacact tgctgtccc 
      ggctttgag cagaagaaa tggcaacag tccaaaata 
      aatcatccg ttcgtttgt gaaccttgt aattgtttc 
      caacacagt aatgagtgc gtctatgac gaagaagtg 
      gatcgcaaa ggattgtct ctggatatt catggcaga 
      tatgcgggc ggaggtgtt tgcctcaat tgccaacat 
      aacactgac ggaatcaat tgcaacaaa tgcaaaccg 
      actttttac cgaccgttt ggcaaattt gagaacgac 
      accgatgct tgtcaaccc tgtaattgt aaccacttt 
      tactccacg ggtaattgt gcggaaggt tcaggccaa 
      tgtgagtgt aaaaaacaa tttcaaccg cccaactgc 
      gacagttgc agttttggg tactttggg tacccaaat 
      tgtagaccg tgtgagtgt tttttgaac ggaaccaaa 
      gatctacag tgtgaggcg cgaaatgga caatgcccg 
      tgtttgtac aactatggg gggcagttc tgtggagag 
      tgtgcacct gggtattac agttttcca gattgtaaa 
      cattgcgag tgcaacccg gaaggttcc gtcacgaat 
      gcttgcgaa caagaaagt ggcaattgc acctgtaag 
      aataatttc ggcggaaag cgatgtgat gagtgccaa 
      cacggttac tacaactac ccaacttgt gactattgc 
      aactgcgac ataaaagga acgaaagaa ggcatttgc 
      gacaaaacc accggccag tgcatgtgc aaagagggc 
      tatacaggc gaccgttgc gattcctgc ctggccggc 
      tactacggc taccccgat tgcaagcct tgcaactgt 
      agcaaggtg ggttcggtt ggtttcagt tgcagcgcc 
      accggaaaa tgctcctgt tcgaccaac tacggcggc 
      aagacttgc gaccagtgc aaccccgga tatttcaaa 
      taccctgaa tgtttacct tgcgaatgc gacagccac 
      ggctccatc ggggtttcg tgcgacggt gaaggcaaa 
      tgcgagtgt caccacaat tttgccggc cttcgttgc 
      gactcgtgc agagaaggt ttctacaat ttcccggct 
      tgtgaggac tgcaactgc catccggct ggagttgtg 
      gctggtttt gccgggtgt gggtcggtc ccagccggg 
      gagctctgc cagtgtaaa gaacgggtc gagggacgg 
      atctgtaat aaatgtagg cccttgtac tggaacttg 
      agtccaaat aatccccaa ggatgcgaa gaatgtaaa 
      tgcaatatt tcgggggtt cttggaggg attgctgtt 
      tgcgactcg gagagtgga gaatgcgtc tgtaaatca 
      ctggtgatg ggtcggggg tgcacggag tgtgtcgat 
      ggcacgtac aacttgcaa gaagagaac ctctttggg 
      tgcaccgat tgtgactgt gatgttggg ggttcggtt 
      aacaatatt tgtgacaag gccaccgga cagtgctta 
      tgtcactcg agggttaaa gggcgcact tgcaaggaa 
      ccacttgaa acgcactat tttccaacc ctttatcag 
      taccagtat gaggttgag gatggacga actccggcg 
      aatacgcta gtgagatat gctaatgat gagcgcacc 
      ttcccgggc tattcctgg aagggatat gccgtgttt 
      ggaccctta caaaaacaa attttgcaa gacgtgatt 
      attctaaaa ccgtcactg tacagaatg gtgttgcgt 
      ttcgtaaac aaaaacccg gaaacagtt attggaaaa 
      attagaata actccggat aatcctagt gataatgag 
      caggaaatc caagtccag tttaggaac agcacagtt 
      ccggcgttc gtaactgtt tcaggcccc gttggaaat 
      actcctcaa ccgtttgtg ataaatccg ggacgttgg 
      cagataagc atcgaagtg acaaaagac ctgcttttg 
      gactatttc gtacttctc cctgaagat ttctacttg 
      gctacgatt ttaaacaaa aaagttgag actccttgc 
      accatcgat ggacctggc ctctgtcac gactacact 
      tatttgaat ttgacgcaa ttttcgacg acttacggc 
      gttggcggg tatgtgcct gaaggtcgc aggtccacc 
      aaacttagg gagtacttc caagacaat gagcatttg 
      gccaaaatt ggggtcgga aaccgggtc ccggtttta 
      aatcttgcc caacctaac gtttcgttt aatatcact 
      gtgcctaaa cccggccag tacgtcatt ataattaat 
      tatgtgacc ccgaaagag gaccaaagg acccacaaa 
      atcgatgtt acactccag tcgggccgg catacaaat 
      gacgggagg attgtgttg tattcgtgt caatacacc 
      acactgtgt cggcaagtt atcaccact cctgagggt 
      atagttgct gtgcagcaa gttgacgga aatgagatt 
      aaagtcgat ttacacagt tctaacgcc gatgttgga 
      atacattcg attgttgca attccatac gaagagtgg 
      tcgttggat tacattaaa ccgaaatcg gtgtgtatt 
      agaaaggac gggaagtgt ttagctgcc tctttccga 
      tatccaccc gaaacgaaa aaaatccag ttcgaggaa 
      aataatgcc gatctcgcg accacactg tggaataac 
      acgtacatg tggttggat ccgagctat gacaccctt 
      gatttgaag ggaaaagtg cccacacct gggtattac 
      acgtttata gtgcactat taccaacct aatttccaa 
      gattttgac gtgactgtc ctcatgcaa aacggtcaa 
      ttttacgaa gcgacttta cccgtccat tactgccca 
      tctcggtca ggctgtcgt tcggttata acccaagtc 
      gatggcaac actaaattt tcattaacc gaaaacttc 
      atgctaaca ttgaaggaa ccaggcaat aaaaacgtc 
      tacctcgac tatttacta gtcatcccg accgacttt 
      tacagcgat cgctacttg gaggaagaa gacgtggat 
      cgcaccggc gaattcatc tccacttgc ggaagcaac 
      cacttcaat atcaacaca acaacggaa ggtttttgc 
      aaagattcg gtattttcc ataaccgcc ggccacaac 
      agcggggct ttgccctgc gggtgcgac tttgccggc 
      tctcttagc ttcgagtgc gacaaattt gggggccag 
      tgccgctgc aaacccaac ataatcggc cgaaagtgt 
      gaggcttgc aagacgggc tatttcggc tttcccgac 
      tgtaaaccg tgcaattgc ccatcgatt gcgttttgc 
      gagcccacc acaggccag tgcatttgc ccaccgcat 
      gtggtgggc gaacgctgc gaccagtgc gagcctctg 
      acctatggg tacgacccc atcattggc tgcgaggag 
      tgcaaatgc aacattctg ggtattgag ggaacggaa 
      cagtgcgac ctcaacaac ggcagttgc cattgcaag 
      ccgaatgtt gtggggaga cagtgcgat aagtgtctg 
      gccggtcat tacgccttc ccttactgc gagttttgc 
      tcttgcgac tacagaggc acgactagt gagatttgc 
      gatcaagcc acggctgag tgtttctgt aaaaagaac 
      gttgtgggg cccacttgt gagatatgt cgcgagggg 
      actttcaat ctgcaagag gagaatgag cacggttgt 
      actgagtgt ttctgtttt gggaaaact acaaggtgt 
      acaagctcc aagttgatt aggacgtct gttcttcaa 
      atggatgat tgggaattg gttgggctt aatttcagt 
      accagactg gaaatctat cctttgaac ttgactgtt 
      cacaatgca gaagagaat tcgatcgga gttgctttc 
      aattcggtt gattttaaa aacaccacc gtatatttt 
      accgcacct agtgattat acagggaag aagttgacg 
      gcttatggg ggttttctc aattatacg ctctactat 
      accataaca ccgggtgaa ggttcggcc gtaagcgag 
      cctgatgtc atccttgag ggcagaaag acctacttg 
      acttattcg agttacgaa caaccgaca gccgcagtt 
      gaatatcac gctagtgtt cagttagtt gaggaaaac 
      tttgagttg cctactggt ttaccggcg agaagggac 
      cacataatg gaggtattg gaggatgtg aggggtgtt 
      tacattagg gccacctac tggactgcg agtgttacg 
      actagattg tctcacgtg attcaagac gacgcaatc 
      tcttatcaa tattattct ggagacaac agaaatttg 
      gcgtttgct ctcacagtg gaagagtgc cgctgtccg 
      gagaattac caaggtttg tcgtgtgaa gaatgcgcc 
      cctggctat taccgggtc gaaggtggc ccgcatggg 
      ggctattgt gtcccgtgc caatgccac ggacacgcc 
      actgaatgc gacgttaac acaggaatt tgtcttaat 
      tgtacgcac aatactcga ggagaccat tgcgaactt 
      tgtgaagtt ggatatcat ggcaatgct ttagccggc 
      actccgatg gattgtttg atttgtgca tgtccttta 
      ccagtcgga agcaataac tttgccaca gcgtgtgat 
      gttagtgcc gatggtgag aaaatcagt tgtgagtgc 
      aaggaggga tattttggg gccaggtgt cagtcgtgt 
      gccgcagga ttttacgga agaccggaa tctttaggt 
      gatttttgc aagccatgt caatgctct ggcaatatc 
      aaccccgac gatcccagc tcttgcgac acagtctct 
      ggtgaatgt ctacgatgt ctaaataac acctacgga 
      aaagcttgt gctctttgt gctcctgga tatttcgga 
      gatgccgtc aatttaaaa gattgtcaa agttgcatt 
      tgcgacgag ctgggcacc gaccactgc aacagtttc 
      accggagaa tgcgtctgc aaacccaac gtaatcggc 
      gaaaaatgt gaccgttgc gaggtgaaa cattacggt 
      ttccaaacg ggggccgga tgtgtccct tgcgattgt 
      gccgaagct tccgatagt tcgcagtgt gacgacgtc 
      acgggtcag tgtagatgc aaacctgga gtctccgga 
      aggtcgtgc aatcgttgc agtgccggc ttttggaac 
      tacacaaca gacggatgt gtttcttgc ggttgcaaa 
      agcgagtat tcgcttggt tttggatgc aacgctttg 
      acgggacaa tgtgagtgc cttcagggg gtcgtgggg 
      gagaaatgc gaccagtgc ccccatcgg tgggtgttt 
      gtcccggat tatggctgt caccagtgt gatagctgt 
      acccacgct ttgcttgac gatacggat gctttgagg 
      cacctgatt gacccaatt attgttgaa tttgactcg 
      gccaactcc gggtacttc acccgccga aagctcgac 
      aacatgaga gatctttta aacgaattg aagccgaag 
      tttgacgaa gtcgacccg aaacagatc aacttggac 
      ttgtacatt gacgagttg caaactctg gaacaggac 
      tcgaagaat ttaaatcga aaaacgaac tattcgcta 
      gaaaatagt gactccctc cgagttagt tctatcgat 
      cttaaagaa agggccgaa cttcttctg gatgatgtt 
      caaaacgct gaagacgat tctttccgc gtaattgac 
      gaaattaat aaaatcacg ttccagttg aacaaagat 
      gaaaatccg gagattgat tcggcgtta gaggaaggc 
      caggaactc ctggatgcg attaaaaga tataattta 
      acaggtcgt gaagaaatg gccgatgac gagttggac 
      aaagtgtcc aacttgctg cttaatgtg tccaacttc 
      aagattcca gtcgattat ttggaagag aaaaccgac 
      aaagtcaag gagagtttg aagaatttt aacgacaag 
      ttggatgat ttgtacaat cagacgcag tactcgtta 
      agcaaagct aacgaggcc cagaggatc attgaaaag 
      agcgggtaa
    mRNA sequence
    • >TC003460 RA
      ttgcggacg aagatgctg tgtctgatt gttgtgccg 
      gcatctcag tagcgtagc gatgttcgt ggtacgcgc 
      gtggacggg ccttttggc cgtccttgt gcttcggct 
      tgaaacagt gcagggtga aattttaac cccgccgta 
      tttcaatct ggcagaggg tagaaaaat tacagcgac 
      tgcgacttg cggagtcga caccgaagg gcccgagct 
      ttactgcaa acttgtggg ggctaatgc cgaaaatga 
      tctaaatgt taatgtgat acaggggca gttttgtga 
      tgtctgcga ccccacaag accggacaa aatgcaccc 
      accggagtt tgctgtgga cggcatgga aacttggtg 
      gcaaagtcc tccactatc tcgaggaat gaaatataa 
      cgaggtcaa cttaaccat tgatctcgg ccaagaatt 
      tcacgtcgc ttacgtttt tattcgaat ggggaattc 
      ccccaggcc cggcttatg ggtgttgga aaaatcggc 
      cgattacgg caaaaccta cacgccttg gcaatattt 
      ctcggattc tcctggcga ttgtgaaac ttactttgg 
      caaagaaag tctgcagcc aatcactaa agacgattc 
      cgtcatttg tacgactga atattcaaa aatagtacc 
      actagaagg cggagaaat cgcaatttc gttgttgaa 
      caaccggcc atcagctaa gaattattt caattctac 
      gattttgca agaatggac ccgagccac caacgtccg 
      attgaagct tttaagaac gaaaaactt cctagggca 
      tctcatgtc ggtggctag acaagatcc gactgttac 
      acgaagata cttttattc gattaaaga tatcagtat 
      tggaggccg ttgcatgtg caatggaca cgctgatac 
      ctgcgatat tcaagactc tcaagaccc gagcgtttt 
      gttatgtcg ctgtcaaca taatacttg tggggcgaa 
      atgtgacac ttgctgtcc cggctttga gcagaagaa 
      atggcaaca gtccaaaat aaatcatcc gttcgtttg 
      tgaaccttg taattgttt ccaacacag taatgagtg 
      cgtctatga cgaagaagt ggatcgcaa aggattgtc 
      tctggatat tcatggcag atatgcggg cggaggtgt 
      ttgcctcaa ttgccaaca taacactga cggaatcaa 
      ttgcaacaa atgcaaacc gacttttta ccgaccgtt 
      tggcaaatt tgagaacga caccgatgc ttgtcaacc 
      ctgtaattg taaccactt ttactccac gggtaattg 
      tgcggaagg ttcaggcca atgtgagtg taaaaaaca 
      atttcaacc gcccaactg cgacagttg cagttttgg 
      gtactttgg gtacccaaa ttgtagacc gtgtgagtg 
      ttttttgaa cggaaccaa agatctaca gtgtgaggc 
      gcgaaatgg acaatgccc gtgtttgta caactatgg 
      ggggcagtt ctgtggaga gtgtgcacc tgggtatta 
      cagttttcc agattgtaa acattgcga gtgcaaccc 
      ggaaggttc cgtcacgaa tgcttgcga acaagaaag 
      tggcaattg cacctgtaa gaataattt cggcggaaa 
      gcgatgtga tgagtgcca acacggtta ctacaacta 
      cccaacttg tgactattg caactgcga cataaaagg 
      aacgaaaga aggcatttg cgacaaaac caccggcca 
      gtgcatgtg caaagaggg ctatacagg cgaccgttg 
      cgattcctg cctggccgg ctactacgg ctaccccga 
      ttgcaagcc ttgcaactg tagcaaggt gggttcggt 
      tggtttcag ttgcagcgc caccggaaa atgctcctg 
      ttcgaccaa ctacggcgg caagacttg cgaccagtg 
      caaccccgg atatttcaa ataccctga atgtttacc 
      ttgcgaatg cgacagcca cggctccat cggggtttc 
      gtgcgacgg tgaaggcaa atgcgagtg tcaccacaa 
      ttttgccgg ccttcgttg cgactcgtg cagagaagg 
      tttctacaa tttcccggc ttgtgagga ctgcaactg 
      ccatccggc tggagttgt ggctggttt tgccgggtg 
      tgggtcggt cccagccgg ggagctctg ccagtgtaa 
      agaacgggt cgagggacg gatctgtaa taaatgtag 
      gcccttgta ctggaactt gagtccaaa taatcccca 
      aggatgcga agaatgtaa atgcaatat ttcgggggt 
      tcttggagg gattgctgt ttgcgactc ggagagtgg 
      agaatgcgt ctgtaaatc actggtgat gggtcgggg 
      gtgcacgga gtgtgtcga tggcacgta caacttgca 
      agaagagaa cctctttgg gtgcaccga ttgtgactg 
      tgatgttgg gggttcggt taacaatat ttgtgacaa 
      ggccaccgg acagtgctt atgtcactc gagggttaa 
      agggcgcac ttgcaagga accacttga aacgcacta 
      ttttccaac cctttatca gtaccagta tgaggttga 
      ggatggacg aactccggc gaatacgct agtgagata 
      tgctaatga tgagcgcac cttcccggg ctattcctg 
      gaagggata tgccgtgtt tggaccctt acaaaaaca 
      aattttgca agacgtgat tattctaaa accgtcact 
      gtacagaat ggtgttgcg tttcgtaaa caaaaaccc 
      ggaaacagt tattggaaa aattagaat aactccgga 
      taatcctag tgataatga gcaggaaat ccaagtcca 
      gtttaggaa cagcacagt tccggcgtt cgtaactgt 
      ttcaggccc cgttggaaa tactcctca accgtttgt 
      gataaatcc gggacgttg gcagataag catcgaagt 
      gacaaaaga cctgctttt ggactattt cgtacttct 
      ccctgaaga tttctactt ggctacgat tttaaacaa 
      aaaagttga gactccttg caccatcga tggacctgg 
      cctctgtca cgactacac ttatttgaa tttgacgca 
      attttcgac gacttacgg cgttggcgg gtatgtgcc 
      tgaaggtcg caggtccac caaacttag ggagtactt 
      ccaagacaa tgagcattt ggccaaaat tggggtcgg 
      aaaccgggt cccggtttt aaatcttgc ccaacctaa 
      cgtttcgtt taatatcac tgtgcctaa acccggcca 
      gtacgtcat tataattaa ttatgtgac cccgaaaga 
      ggaccaaag gacccacaa aatcgatgt tacactcca 
      gtcgggccg gcatacaaa tgacgggag gattgtgtt 
      gtattcgtg tcaatacac cacactgtg tcggcaagt 
      tatcaccac tcctgaggg tatagttgc tgtgcagca 
      agttgacgg aaatgagat taaagtcga tttacacag 
      ttctaacgc cgatgttgg aatacattc gattgttgc 
      aattccata cgaagagtg gtcgttgga ttacattaa 
      accgaaatc ggtgtgtat tagaaagga cgggaagtg 
      tttagctgc ctctttccg atatccacc cgaaacgaa 
      aaaaatcca gttcgagga aaataatgc cgatctcgc 
      gaccacact gtggaataa cacgtacat gtggttgga 
      tccgagcta tgacaccct tgatttgaa gggaaaagt 
      gcccacacc tgggtatta cacgtttat agtgcacta 
      ttaccaacc taatttcca agattttga cgtgactgt 
      cctcatgca aaacggtca attttacga agcgacttt 
      acccgtcca ttactgccc atctcggtc aggctgtcg 
      ttcggttat aacccaagt cgatggcaa cactaaatt 
      ttcattaac cgaaaactt catgctaac attgaagga 
      accaggcaa taaaaacgt ctacctcga ctatttact 
      agtcatccc gaccgactt ttacagcga tcgctactt 
      ggaggaaga agacgtgga tcgcaccgg cgaattcat 
      ctccacttg cggaagcaa ccacttcaa tatcaacac 
      aacaacgga aggtttttg caaagattc ggtattttc 
      cataaccgc cggccacaa cagcggggc tttgccctg 
      cgggtgcga ctttgccgg ctctcttag cttcgagtg 
      cgacaaatt tgggggcca gtgccgctg caaacccaa 
      cataatcgg ccgaaagtg tgaggcttg caagacggg 
      ctatttcgg ctttcccga ctgtaaacc gtgcaattg 
      cccatcgat tgcgttttg cgagcccac cacaggcca 
      gtgcatttg cccaccgca tgtggtggg cgaacgctg 
      cgaccagtg cgagcctct gacctatgg gtacgaccc 
      catcattgg ctgcgagga gtgcaaatg caacattct 
      gggtattga gggaacgga acagtgcga cctcaacaa 
      cggcagttg ccattgcaa gccgaatgt tgtggggag 
      acagtgcga taagtgtct ggccggtca ttacgcctt 
      cccttactg cgagttttg ctcttgcga ctacagagg 
      cacgactag tgagatttg cgatcaagc cacggctga 
      gtgtttctg taaaaagaa cgttgtggg gcccacttg 
      tgagatatg tcgcgaggg gactttcaa tctgcaaga 
      ggagaatga gcacggttg tactgagtg tttctgttt 
      tgggaaaac tacaaggtg tacaagctc caagttgat 
      taggacgtc tgttcttca aatggatga ttgggaatt 
      ggttgggct taatttcag taccagact ggaaatcta 
      tcctttgaa cttgactgt tcacaatgc agaagagaa 
      ttcgatcgg agttgcttt caattcggt tgattttaa 
      aaacaccac cgtatattt taccgcacc tagtgatta 
      tacagggaa gaagttgac ggcttatgg gggttttct 
      caattatac gctctacta taccataac accgggtga 
      aggttcggc cgtaagcga gcctgatgt catccttga 
      gggcagaaa gacctactt gacttattc gagttacga 
      acaaccgac agccgcagt tgaatatca cgctagtgt 
      tcagttagt tgaggaaaa ctttgagtt gcctactgg 
      tttaccggc gagaaggga ccacataat ggaggtatt 
      ggaggatgt gaggggtgt ttacattag ggccaccta 
      ctggactgc gagtgttac gactagatt gtctcacgt 
      gattcaaga cgacgcaat ctcttatca atattattc 
      tggagacaa cagaaattt ggcgtttgc tctcacagt 
      ggaagagtg ccgctgtcc ggagaatta ccaaggttt 
      gtcgtgtga agaatgcgc ccctggcta ttaccgggt 
      cgaaggtgg cccgcatgg gggctattg tgtcccgtg 
      ccaatgcca cggacacgc cactgaatg cgacgttaa 
      cacaggaat ttgtcttaa ttgtacgca caatactcg 
      aggagacca ttgcgaact ttgtgaagt tggatatca 
      tggcaatgc tttagccgg cactccgat ggattgttt 
      gatttgtgc atgtccttt accagtcgg aagcaataa 
      ctttgccac agcgtgtga tgttagtgc cgatggtga 
      gaaaatcag ttgtgagtg caaggaggg atattttgg 
      ggccaggtg tcagtcgtg tgccgcagg attttacgg 
      aagaccgga atctttagg tgatttttg caagccatg 
      tcaatgctc tggcaatat caaccccga cgatcccag 
      ctcttgcga cacagtctc tggtgaatg tctacgatg 
      tctaaataa cacctacgg aaaagcttg tgctctttg 
      tgctcctgg atatttcgg agatgccgt caatttaaa 
      agattgtca aagttgcat ttgcgacga gctgggcac 
      cgaccactg caacagttt caccggaga atgcgtctg 
      caaacccaa cgtaatcgg cgaaaaatg tgaccgttg 
      cgaggtgaa acattacgg tttccaaac gggggccgg 
      atgtgtccc ttgcgattg tgccgaagc ttccgatag 
      ttcgcagtg tgacgacgt cacgggtca gtgtagatg 
      caaacctgg agtctccgg aaggtcgtg caatcgttg 
      cagtgccgg cttttggaa ctacacaac agacggatg 
      tgtttcttg cggttgcaa aagcgagta ttcgcttgg 
      ttttggatg caacgcttt gacgggaca atgtgagtg 
      ccttcaggg ggtcgtggg ggagaaatg cgaccagtg 
      cccccatcg gtgggtgtt tgtcccgga ttatggctg 
      tcaccagtg tgatagctg tacccacgc tttgcttga 
      cgatacgga tgctttgag gcacctgat tgacccaat 
      tattgttga atttgactc ggccaactc cgggtactt 
      cacccgccg aaagctcga caacatgag agatctttt 
      aaacgaatt gaagccgaa gtttgacga agtcgaccc 
      gaaacagat caacttgga cttgtacat tgacgagtt 
      gcaaactct ggaacagga ctcgaagaa tttaaatcg 
      aaaaacgaa ctattcgct agaaaatag tgactccct 
      ccgagttag ttctatcga tcttaaaga aagggccga 
      acttcttct ggatgatgt tcaaaacgc tgaagacga 
      ttctttccg cgtaattga cgaaattaa taaaatcac 
      gttccagtt gaacaaaga tgaaaatcc ggagattga 
      ttcggcgtt agaggaagg ccaggaact cctggatgc 
      gattaaaag atataattt aacaggtcg tgaagaaat 
      ggccgatga cgagttgga caaagtgtc caacttgct 
      gcttaatgt gtccaactt caagattcc agtcgatta 
      tttggaaga gaaaaccga caaagtcaa ggagagttt 
      gaagaattt taacgacaa gttggatga tttgtacaa 
      tcagacgca gtactcgtt aagcaaagc taacgaggc 
      ccagaggat cattgaaaa gagcgggta agtacaaac 
      aaattaata aaagacgaa cttattt