Status
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Gene information
General information
TC003457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5375625..5378015
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003457
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03559

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003457 RA
      MSDTEEKQK YPKQQIAAI LTMTVFKKL CLVGIRTAL 
      YAYLENIAK YSKDDAEMV YNAFRSFNY CCPLLGALI 
      ADCFIGKFY TIVFFLIAY TIGTLVLSG SAVQFSEDI 
      SLPLFFFSL GLITLSVGV IKPCIAAFG GDQFKLPEQ 
      HKHLEHFFL LAYVAVNTG AAIGIYEAP NLRKMHCFG 
      ADSCYSAAF LLSTVSIII SLIVFVAAK RWYVCVKPQ 
      RYNTVLWVL CMLNGVKEK IVSKEKQEH WLDYSKKSY 
      GESFVRQTK NTLKVVVIF TVLQFHGAT VIITMTGWS 
      TQSHFMRNE QGGRVFNPS TIRFLNYVA VILVTPILC 
      LIVLFLRSK CHIRVTPLQ RIACGAFIA SVTMFVAAV 
      LSLIIEAEV PNLPGQGEC HIRFYNPLN CAVKIDAPP 
      FIDKVQIDP MGYKFISVK VKGTKKLKH TISGCDKTI 
      SNTTGPFVV VEEVSLGYF TTTNGLQAF KEDLEKDAD 
      GDAKIRSLV GSLGKTDEK IKYGCANDH VIKIASQDY 
      SVHKVDYGF YDIGGVKDA ARFFRGGIY RVLLYFQDN 
      KVGNVTFYE VGKPHQLHI LWQIPQCVL HAISRVLVT 
      PAISEFAYT QTPFKSLKC TITAIGLMA TGVGGIIVI 
      LIELMHLKQ SHYYFTFGG FTMLFTLIF IFLAVRYRY 
      VDQRYGKEE NILTEDTES KDE
    CDS sequence
    • >TC003457 RA
      atgagtgac actgaagag aaacaaaaa tatccgaag 
      cagcaaatt gctgcgatt cttacaatg acagtcttc 
      aaaaaactg tgtttggta ggaatacga acggcttta 
      tatgcctac ttggagaat atcgcaaag tacagtaaa 
      gacgacgct gaaatggta tataacgcc tttcgcagc 
      tttaactat tgttgtccc ttgctgggg gcattaatt 
      gccgactgt tttattgga aaattttac acaattgtg 
      tttttcctg attgcttac acaatcgga acattggtg 
      ttgtcagga tcggctgtc caattctca gaggacatt 
      tcgctaccg cttttcttc ttctccctt ggtttgatt 
      accctgagt gtgggggtg attaaaccg tgcattgct 
      gcgttcgga ggagaccag tttaagtta ccggaacag 
      cacaagcac ctggaacat ttctttttg ttggcttac 
      gttgctgtg aacacggga gcagcaatt ggcatttac 
      gaagcacca aatcttcga aaaatgcat tgctttggg 
      gctgatagt tgttattca gctgcgttt ctattatcc 
      actgttagc atcattatc agcttaatt gtatttgtg 
      gcggcgaaa cgctggtac gtctgtgtc aagccacaa 
      aggtataat accgtcctt tgggtgttg tgtatgctg 
      aacggagtt aaggaaaaa atcgtttcg aaagaaaaa 
      caagagcac tggttggac tactctaaa aaatcgtat 
      ggagagtcg tttgtgaga caaacaaaa aatacctta 
      aaagtggtt gtgattttt acggttctt caatttcat 
      ggggccact gttattatt acgatgact ggttggagc 
      acacagtca cactttatg cgaaatgaa caaggaggc 
      agagttttt aacccgtca acaattcgg ttcctgaat 
      tatgtagct gtgattctt gttacgcca atactgtgt 
      ctgattgtg ttgttttta cgttcaaaa tgccacatc 
      cgtgttaca ccacttcag cgtatagcc tgtggtgcc 
      tttattgcg tccgtaact atgttcgta gcagccgtt 
      ttatcttta ataatagaa gccgaagtt ccaaatttg 
      ccaggacaa ggagaatgt catatcaga ttttacaac 
      cctcttaac tgtgcagtt aaaatagac gctcctcct 
      ttcatagat aaggtgcaa attgatcca atgggatac 
      aagtttatc tcagtcaaa gtgaaaggt accaagaag 
      ttaaagcat acgatttct gggtgtgat aaaacaatt 
      tcaaacacc accggccca tttgttgtg gtcgaagaa 
      gtgagcttg ggttatttt accacgaca aatggtcta 
      caagccttt aaagaagat ttggaaaaa gatgcggat 
      ggtgatgca aaaatcaga agtttagtt ggaagtttg 
      ggaaaaaca gacgagaaa atcaaatat ggttgtgca 
      aatgatcat gtcattaaa attgcgtct caggattat 
      tcggtgcat aaggttgac tatggtttt tacgatatt 
      ggaggtgtt aaagacgct gcaaggttt tttcgtggt 
      ggcatttat agagttttg ctttatttc caggataat 
      aaagtcggg aacgtcacc ttttatgaa gttgggaaa 
      ccacatcag ttacacatt ttgtggcaa attccccag 
      tgtgtgctc cacgccata agcagggtt ttggtaaca 
      ccagcaatt tcagaattt gcatataca caaacgcct 
      tttaagtcg ttgaaatgc accatcact gcaattgga 
      ttgatggca acaggggta ggaggaatt atagttata 
      ctgatagaa ttgatgcat cttaaacag tcgcattat 
      tactttaca tttggggga tttacgatg ctgtttaca 
      ttaattttt attttctta gcagttaga tatagatat 
      gtggatcaa cggtacggt aaagaagaa aatatatta 
      actgaagat accgagagc aaagatgag tag
    mRNA sequence
    • >TC003457 RA
      tacgacagc taatacctg ataaaaaaa tattaactt 
      cttattacc tcaagcaaa atgagtgac actgaagag 
      aaacaaaaa tatccgaag cagcaaatt gctgcgatt 
      cttacaatg acagtcttc aaaaaactg tgtttggta 
      ggaatacga acggcttta tatgcctac ttggagaat 
      atcgcaaag tacagtaaa gacgacgct gaaatggta 
      tataacgcc tttcgcagc tttaactat tgttgtccc 
      ttgctgggg gcattaatt gccgactgt tttattgga 
      aaattttac acaattgtg tttttcctg attgcttac 
      acaatcgga acattggtg ttgtcagga tcggctgtc 
      caattctca gaggacatt tcgctaccg cttttcttc 
      ttctccctt ggtttgatt accctgagt gtgggggtg 
      attaaaccg tgcattgct gcgttcgga ggagaccag 
      tttaagtta ccggaacag cacaagcac ctggaacat 
      ttctttttg ttggcttac gttgctgtg aacacggga 
      gcagcaatt ggcatttac gaagcacca aatcttcga 
      aaaatgcat tgctttggg gctgatagt tgttattca 
      gctgcgttt ctattatcc actgttagc atcattatc 
      agcttaatt gtatttgtg gcggcgaaa cgctggtac 
      gtctgtgtc aagccacaa aggtataat accgtcctt 
      tgggtgttg tgtatgctg aacggagtt aaggaaaaa 
      atcgtttcg aaagaaaaa caagagcac tggttggac 
      tactctaaa aaatcgtat ggagagtcg tttgtgaga 
      caaacaaaa aatacctta aaagtggtt gtgattttt 
      acggttctt caatttcat ggggccact gttattatt 
      acgatgact ggttggagc acacagtca cactttatg 
      cgaaatgaa caaggaggc agagttttt aacccgtca 
      acaattcgg ttcctgaat tatgtagct gtgattctt 
      gttacgcca atactgtgt ctgattgtg ttgttttta 
      cgttcaaaa tgccacatc cgtgttaca ccacttcag 
      cgtatagcc tgtggtgcc tttattgcg tccgtaact 
      atgttcgta gcagccgtt ttatcttta ataatagaa 
      gccgaagtt ccaaatttg ccaggacaa ggagaatgt 
      catatcaga ttttacaac cctcttaac tgtgcagtt 
      aaaatagac gctcctcct ttcatagat aaggtgcaa 
      attgatcca atgggatac aagtttatc tcagtcaaa 
      gtgaaaggt accaagaag ttaaagcat acgatttct 
      gggtgtgat aaaacaatt tcaaacacc accggccca 
      tttgttgtg gtcgaagaa gtgagcttg ggttatttt 
      accacgaca aatggtcta caagccttt aaagaagat 
      ttggaaaaa gatgcggat ggtgatgca aaaatcaga 
      agtttagtt ggaagtttg ggaaaaaca gacgagaaa 
      atcaaatat ggttgtgca aatgatcat gtcattaaa 
      attgcgtct caggattat tcggtgcat aaggttgac 
      tatggtttt tacgatatt ggaggtgtt aaagacgct 
      gcaaggttt tttcgtggt ggcatttat agagttttg 
      ctttatttc caggataat aaagtcggg aacgtcacc 
      ttttatgaa gttgggaaa ccacatcag ttacacatt 
      ttgtggcaa attccccag tgtgtgctc cacgccata 
      agcagggtt ttggtaaca ccagcaatt tcagaattt 
      gcatataca caaacgcct tttaagtcg ttgaaatgc 
      accatcact gcaattgga ttgatggca acaggggta 
      ggaggaatt atagttata ctgatagaa ttgatgcat 
      cttaaacag tcgcattat tactttaca tttggggga 
      tttacgatg ctgtttaca ttaattttt attttctta 
      gcagttaga tatagatat gtggatcaa cggtacggt 
      aaagaagaa aatatatta actgaagat accgagagc 
      aaagatgag taggatttc aaaaataat aaatagttt 
      taaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY