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Gene information
General information
TC003453 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5332229..5350543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003453
    iBeetle screen iB_03558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003453 RA
      MTDSKLGME SVSPVTLSS DWSRLARLI FLASLAAVG 
      SVGNVFMIS AVMIEDYLR KRGNTFVVS VAFADLLVT 
      GLVIPASAV VILAGLPEN LPVCKFQWF LATLCFLVT 
      VLSLTAIAA ENYVRLCYS STTYDLLSR SKITTILLL 
      LWIVSSVTS SLQFATDLT FDYCTRKNP GLIPYQATI 
      GVVFVFLPI ILTFFSYVR TAYHIRRAK TRPNFKPPV 
      TFNWDYSLM HSNLYSFLL FVVFWLPFG VVLAVGTVK 
      EVHNSLFYK LAWLGITKS CINSILYCI TNRHFRGAY 
      VNLFHYCCC KTTVTFSRR QRPEGVRPS GDVRVHIIP 
      GYNMYCSPQ RSREGQSKR CSSRPNGRD VYEL
    CDS sequence
    • >TC003453 RA
      atgacggac tccaagctg gggatggag tcggtgtcg 
      ccggtgaca ctgagttct gactggtcc cgactggcc 
      cgacttata ttccttgct tctcttgcc gctgtcgga 
      agcgttggt aatgtgttt atgataagc gccgtcatg 
      attgaagat tacctccgg aagcgaggt aacacgttc 
      gtggtaagc gtggcattc gccgacctg ctggtgacg 
      ggactagta atccctgca tccgctgtc gtgatactg 
      gcaggcctt ccggagaac ctccccgtg tgcaaattc 
      cagtggttc ctcgcgacg ctgtgtttc ctggtaaca 
      gtcctctct ctcacagca atcgccgcc gaaaactac 
      gtccgtctg tgctactcc tccacgacc tacgacctg 
      ttgagccgc agcaaaatc accacaatt ctcctactt 
      ttgtggatc gtaagttcc gtcacttcg agcctccaa 
      ttcgccact gacttgacc ttcgactac tgcaccagg 
      aagaacccc ggcctgatc ccgtaccag gcgaccatt 
      ggcgtggtg tttgtgttc ttgcccata atcttgacg 
      ttttttagt tacgtgcgg accgcatat cacatacgc 
      agggccaaa acgcggccc aacttcaaa ccccccgtc 
      actttcaac tgggactac tcgctcatg cacagcaat 
      ctgtacagt tttctttta tttgtggtc ttctggctt 
      ccgtttggc gtggttctt gcggtcgga actgtgaag 
      gaagttcac aacagtttg ttttataag ctggcctgg 
      ctggggatc accaaatct tgcattaac agcattttg 
      tactgcatt accaacaga cactttcga ggggcttac 
      gttaacttg tttcactac tgctgctgc aaaaccacg 
      gtgaccttc tccaggagg cagcggccg gagggcgtc 
      cggccctcc ggggacgtc cgggtccac atcattcca 
      ggttataac atgtactgc agtccgcag aggagccgc 
      gaggggcag agcaagagg tgctcgtca cgacccaac 
      ggcagagac gtctacgaa ttatga
    mRNA sequence
    • >TC003453 RA
      gcctggacg ctccacacc gtcgttgct ctgataacg 
      accaatttc tgattagtg gcaagtgtt ttagtgtgt 
      taatacaga ctccatcca attaatcac aagaaatgc 
      cgcgcttgc cttaagccg catcctcgt ctacctggt 
      ccgtggaca tggtgtcga acatgacgg actccaagc 
      tggggatgg agtcggtgt cgccggtga cactgagtt 
      ctgactggt cccgactgg cccgactta tattccttg 
      cttctcttg ccgctgtcg gaagcgttg gtaatgtgt 
      ttatgataa gcgccgtca tgattgaag attacctcc 
      ggaagcgag gtaacacgt tcgtggtaa gcgtggcat 
      tcgccgacc tgctggtga cgggactag taatccctg 
      catccgctg tcgtgatac tggcaggcc ttccggaga 
      acctccccg tgtgcaaat tccagtggt tcctcgcga 
      cgctgtgtt tcctggtaa cagtcctct ctctcacag 
      caatcgccg ccgaaaact acgtccgtc tgtgctact 
      cctccacga cctacgacc tgttgagcc gcagcaaaa 
      tcaccacaa ttctcctac ttttgtgga tcgtaagtt 
      ccgtcactt cgagcctcc aattcgcca ctgacttga 
      ccttcgact actgcacca ggaagaacc ccggcctga 
      tcccgtacc aggcgacca ttggcgtgg tgtttgtgt 
      tcttgccca taatcttga cgtttttta gttacgtgc 
      ggaccgcat atcacatac gcagggcca aaacgcggc 
      ccaacttca aaccccccg tcactttca actgggact 
      actcgctca tgcacagca atctgtaca gttttcttt 
      tatttgtgg tcttctggc ttccgtttg gcgtggttc 
      ttgcggtcg gaactgtga aggaagttc acaacagtt 
      tgttttata agctggcct ggctgggga tcaccaaat 
      cttgcatta acagcattt tgtactgca ttaccaaca 
      gacactttc gaggggctt acgttaact tgtttcact 
      actgctgct gcaaaacca cggtgacct tctccagga 
      ggcagcggc cggagggcg tccggccct ccggggacg 
      tccgggtcc acatcattc caggttata acatgtact 
      gcagtccgc agaggagcc gcgaggggc agagcaaga 
      ggtgctcgt cacgaccca acggcagag acgtctacg 
      aattatgat gagacgatt attacacca ccagcagag 
      gggtgtgga tttagataa caaatttat caagaattt 
      ttactgaaa gatttttgg tggtaatcg aatcctgcg 
      aactgagct ttattcctg ctgtactta atacgaaat 
      tattgtgta tgcacaaaa ccggcaaac attccggaa 
      cgtcagtat tttgttgac tgtttgagc ttattatta 
      gaaaaacat tcctttaat caagttgat taattttag 
      tgctgtaca aaccgaaac tcgaacaga ttttaaagc 
      atttgtaaa catgtttca aagtttgct aaattcaaa 
      taaacctct taatcgatg catcttgga tcttaattt 
      actgcacac gcgcagttt ttaacgcct ttttaaagc 
      tttaatgaa tcgttatgg aaattcctt caaaagact 
      gactcgtaa tttaacaca aaacaactt gcgatgtgt 
      cagaattta cacacaact ttgctgtta ttaccttgc 
      cttattaaa ttaaaaaca tctttccac gtttattca 
      ttaaaagta caaagtgca atgttgtta ttttattgc 
      atctcgact tattaaaag tttatttac aagttccat 
      tgtttaaag agagtctat tacgtactt ttattaaag 
      tctgcaaag tgtaaataa agtgaataa tatttattt 
      tacgacaaa aactactca caacaatct ttgacccgg 
      cgcctccac catttttaa cgcacatgt aaagagtcc 
      gctgcaaca taaagaccg tccatagca ttatttgaa 
      acgttatat taaaaatat aaactacaa aacattgaa 
      aagtacatt tagcatttt acagtgcca taaaaatac 
      aaaattaaa gtaacctag ctccttgtt aaaattttg 
      atttgtaat ttaaaaata aatcctcta aagtagtta 
      tttgttgcc aaaacgcta aatgggctt ttcggtcac 
      ctccactga cgcacaaac tttctttaa gacactgtt 
      gaattgtta tgattgcta gctttaaat accacttag 
      gatagtttt gtttaattg ttggtctgg caaaagtcc 
      ggccaggat aagaatcgg tctcgtatt gttttatgc 
      aataatatg agaaataag atccaagat tcatcacgt 
      ggactatat gtttgattc gatttgcac ttggaaaaa 
      aacatggca tccgctttt gtgaaaaaa tcaaaatat 
      agttcaaaa tttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY