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Gene information
General information
TC003433 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 584870..587216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003433
    iBeetle screen iB_03556
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003433 RA
      MVRRHNNQL PENLPQLQN LIKRDPASY HEEFTQQYQ 
      HFQNTIQVF ELSPDQTNK TLDDLVMFM AQVSHCYPK 
      ELINFPQQL IDLLEKNST VLNATMRMT FCRALILLR 
      NKNLLAPTD LLELFFKLL RCQDKALRS FLETHIITD 
      IKNLNAKHK NAKLNTTLQ NFMFTMLKD TNSRAAKMS 
      VDIMIELYK KNVWNDAKT VNVIASGCF SKFTKVMVA 
      SMKFFLGKD SDEKDSDSD SDEEIDPKE VMMANKVNK 
      KTRKREKQL SKVKKLASK AYKKKSAAP VFNFSALHL 
      IHDPQGMAE KLFNQMETT NKRFEIKIL TLDVISRLI 
      GLHQLFLFS YYPYIQRFL QPHQREVTR ILQFAAQAS 
      HELVPPDVI EPVLKTLAN NFITERNSS DVMAIGLNA 
      VREICSRCP LAMNEDLLR DLAMYKNYR DRAVMMAAK 
      SLIHLFRNT RPELLHKKD RGRPTEATE ELGSITYGQ 
      VDAKDYVPG AEVLLKEDD KEVEINSES GSDDEEWVD 
      IKHSSDEEG ESEDNCSEE EEEKEEEEK SESPERKIL 
      KAKSSKREL AHKVTIDRI LTDEDFKKI DMANIRKQV 
      QGPKKGTKR KIEEVKVSG ASELVKLGD IENIYKKRK 
      HDKQTRIES VKAGQVDRE KFGYKDGRM NIHCSKTNR 
      EKRKTKNYQ MVKHKMQRK GKRSFKDKQ IALRNHLLK 
      LKKMK
    CDS sequence
    • >TC003433 RA
      atggtccga agacacaat aaccagctg cctgaaaac 
      ctcccccag ctgcaaaat ctcatcaaa cgagacccc 
      gcctcatac catgaagaa ttcacacaa cagtatcaa 
      catttccaa aacacaatt caagttttt gagttaagc 
      cccgaccaa actaacaaa actttggac gacctggtc 
      atgttcatg gcacaagtg tcgcactgt taccccaag 
      gaactcatc aactttccc caacagctg atcgatttg 
      ttggaaaaa aatagcaca gttttaaac gccaccatg 
      agaatgact ttttgccgg gctcttatc ctcctccgt 
      aacaagaat ttgttagcc cccactgac ctactggaa 
      ttatttttc aagcttctg aggtgccaa gacaaggcc 
      ttgaggtcg ttcttggaa actcatatc atcacagat 
      atcaagaac ttgaacgcg aaacataaa aatgccaag 
      ttgaacaca accttacag aattttatg tttacaatg 
      ctgaaggac actaattca agggctgcg aaaatgtca 
      gtcgatatc atgattgaa ctttacaag aagaacgtt 
      tggaatgat gccaaaaca gtgaacgtt attgcgtca 
      ggttgtttc tcaaaattc acaaaagta atggtagct 
      tccatgaag tttttccta gggaaggac tctgacgag 
      aaagattca gactccgat tctgatgaa gaaatagac 
      cccaaagaa gtgatgatg gccaacaaa gtcaacaaa 
      aagacaaga aaaagggaa aaacagttg agtaaagta 
      aaaaaattg gcatcaaag gcttacaag aagaaatca 
      gcagcgcca gtttttaat ttttctgct ttacatttg 
      atccatgac ccacaaggt atggcggaa aaactcttc 
      aaccagatg gaaacaaca aataaacga tttgaaatc 
      aaaatttta acactcgat gttatatca cggctcatt 
      ggcttgcat cagttgttt ttgttcagt tattacccg 
      tacattcag agatttctc caaccgcat cagcgtgaa 
      gtgacaaga atattacaa tttgcggca caagcaagt 
      catgagttg gtccctcca gacgttata gagcctgtt 
      ttgaaaact ttagccaac aattttatt actgaaaga 
      aattcctcg gatgttatg gctatagga ctgaatgca 
      gtgcgtgag atatgctct aggtgtcct cttgcgatg 
      aatgaagac ctactgcgt gacttggca atgtataaa 
      aactacaga gatcgcgct gtcatgatg gccgcaaaa 
      tctctcatc cacttattc agaaataca aggcctgag 
      ttactgcat aagaaagac agggggcgc cctactgaa 
      gcaactgaa gaacttggg tccataact tatggacaa 
      gttgatgcc aaggattat gtaccaggg gctgaagtg 
      cttttgaaa gaagatgac aaggaggtt gagattaat 
      tcagaatct ggttctgat gatgaggaa tgggttgat 
      attaaacac tcttctgat gaagaaggc gaaagtgaa 
      gacaattgc agtgaagaa gaggaggaa aaagaagaa 
      gaagaaaaa agcgaatca ccggaacgc aaaatattg 
      aaagccaag tcttccaaa agagagctg gcgcacaaa 
      gtcaccatc gaccgcatc ctaactgat gaagacttc 
      aagaaaatt gacatggcc aacatcaga aaacaggtc 
      cagggccct aagaaggga acaaagcgg aaaatcgaa 
      gaagttaaa gtgagtggg gcgtccgaa cttgtcaaa 
      ttgggtgat attgaaaac atatacaaa aaacgaaag 
      catgataaa cagacaagg attgaaagt gttaaagcg 
      ggccaagtg gatcgagag aagtttggc tacaaggac 
      ggaagaatg aacattcac tgctcgaaa acaaaccgg 
      gagaagagg aagacgaaa aactaccaa atggtcaaa 
      cacaaaatg caaaggaag ggaaaacgc agcttcaaa 
      gacaagcaa attgcgtta aggaatcac ttgttaaag 
      ttgaaaaag atgaaataa
    mRNA sequence
    • >TC003433 RA
      tgaataaaa aaattcgtt cacgtaatt tatctacaa 
      gtaaagttt ttagatttt gttatgttg gtgctagtg 
      ttaggctcg ttttttagg ttaggtgtt ataacctca 
      attgcacgt gttataaaa ttgcgaaaa tggtccgaa 
      gacacaata accagctgc ctgaaaacc tcccccagc 
      tgcaaaatc tcatcaaac gagaccccg cctcatacc 
      atgaagaat tcacacaac agtatcaac atttccaaa 
      acacaattc aagtttttg agttaagcc ccgaccaaa 
      ctaacaaaa ctttggacg acctggtca tgttcatgg 
      cacaagtgt cgcactgtt accccaagg aactcatca 
      actttcccc aacagctga tcgatttgt tggaaaaaa 
      atagcacag ttttaaacg ccaccatga gaatgactt 
      tttgccggg ctcttatcc tcctccgta acaagaatt 
      tgttagccc ccactgacc tactggaat tatttttca 
      agcttctga ggtgccaag acaaggcct tgaggtcgt 
      tcttggaaa ctcatatca tcacagata tcaagaact 
      tgaacgcga aacataaaa atgccaagt tgaacacaa 
      ccttacaga attttatgt ttacaatgc tgaaggaca 
      ctaattcaa gggctgcga aaatgtcag tcgatatca 
      tgattgaac tttacaaga agaacgttt ggaatgatg 
      ccaaaacag tgaacgtta ttgcgtcag gttgtttct 
      caaaattca caaaagtaa tggtagctt ccatgaagt 
      ttttcctag ggaaggact ctgacgaga aagattcag 
      actccgatt ctgatgaag aaatagacc ccaaagaag 
      tgatgatgg ccaacaaag tcaacaaaa agacaagaa 
      aaagggaaa aacagttga gtaaagtaa aaaaattgg 
      catcaaagg cttacaaga agaaatcag cagcgccag 
      tttttaatt tttctgctt tacatttga tccatgacc 
      cacaaggta tggcggaaa aactcttca accagatgg 
      aaacaacaa ataaacgat ttgaaatca aaattttaa 
      cactcgatg ttatatcac ggctcattg gcttgcatc 
      agttgtttt tgttcagtt attacccgt acattcaga 
      gatttctcc aaccgcatc agcgtgaag tgacaagaa 
      tattacaat ttgcggcac aagcaagtc atgagttgg 
      tccctccag acgttatag agcctgttt tgaaaactt 
      tagccaaca attttatta ctgaaagaa attcctcgg 
      atgttatgg ctataggac tgaatgcag tgcgtgaga 
      tatgctcta ggtgtcctc ttgcgatga atgaagacc 
      tactgcgtg acttggcaa tgtataaaa actacagag 
      atcgcgctg tcatgatgg ccgcaaaat ctctcatcc 
      acttattca gaaatacaa ggcctgagt tactgcata 
      agaaagaca gggggcgcc ctactgaag caactgaag 
      aacttgggt ccataactt atggacaag ttgatgcca 
      aggattatg taccagggg ctgaagtgc ttttgaaag 
      aagatgaca aggaggttg agattaatt cagaatctg 
      gttctgatg atgaggaat gggttgata ttaaacact 
      cttctgatg aagaaggcg aaagtgaag acaattgca 
      gtgaagaag aggaggaaa aagaagaag aagaaaaaa 
      gcgaatcac cggaacgca aaatattga aagccaagt 
      cttccaaaa gagagctgg cgcacaaag tcaccatcg 
      accgcatcc taactgatg aagacttca agaaaattg 
      acatggcca acatcagaa aacaggtcc agggcccta 
      agaagggaa caaagcgga aaatcgaag aagttaaag 
      tgagtgggg cgtccgaac ttgtcaaat tgggtgata 
      ttgaaaaca tatacaaaa aacgaaagc atgataaac 
      agacaagga ttgaaagtg ttaaagcgg gccaagtgg 
      atcgagaga agtttggct acaaggacg gaagaatga 
      acattcact gctcgaaaa caaaccggg agaagagga 
      agacgaaaa actaccaaa tggtcaaac acaaaatgc 
      aaaggaagg gaaaacgca gcttcaaag acaagcaaa 
      ttgcgttaa ggaatcact tgttaaagt tgaaaaaga 
      tgaaataat tttgtattt atcgttttg ttatttaat 
      aaatgaaaa aaatcact