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Gene information
General information
TC003433 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5146157..5148503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003433
    iBeetle screen iB_03556

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003433 RA
      MVRRHNNQL PENLPQLQN LIKRDPASY HEEFTQQYQ 
HFQNTIQVF ELSPDQTNK TLDDLVMFM AQVSHCYPK 
ELINFPQQL IDLLEKNST VLNATMRMT FCRALILLR 
NKNLLAPTD LLELFFKLL RCQDKALRS FLETHIITD 
IKNLNAKHK NAKLNTTLQ NFMFTMLKD TNSRAAKMS 
VDIMIELYK KNVWNDAKT VNVIASGCF SKFTKVMVA 
SMKFFLGKD SDEKDSDSD SDEEIDPKE VMMANKVNK 
KTRKREKQL SKVKKLASK AYKKKSAAP VFNFSALHL 
IHDPQGMAE KLFNQMETT NKRFEIKIL TLDVISRLI 
GLHQLFLFS YYPYIQRFL QPHQREVTR ILQFAAQAS 
HELVPPDVI EPVLKTLAN NFITERNSS DVMAIGLNA 
VREICSRCP LAMNEDLLR DLAMYKNYR DRAVMMAAK 
SLIHLFRNT RPELLHKKD RGRPTEATE ELGSITYGQ 
VDAKDYVPG AEVLLKEDD KEVEINSES GSDDEEWVD 
IKHSSDEEG ESEDNCSEE EEEKEEEEK SESPERKIL 
KAKSSKREL AHKVTIDRI LTDEDFKKI DMANIRKQV 
QGPKKGTKR KIEEVKVSG ASELVKLGD IENIYKKRK 
HDKQTRIES VKAGQVDRE KFGYKDGRM NIHCSKTNR 
EKRKTKNYQ MVKHKMQRK GKRSFKDKQ IALRNHLLK 
LKKMK
    CDS sequence
    • >TC003433 RA
      atggtccga agacacaat aaccagctg cctgaaaac 
ctcccccag ctgcaaaat ctcatcaaa cgagacccc 
gcctcatac catgaagaa ttcacacaa cagtatcaa 
catttccaa aacacaatt caagttttt gagttaagc 
cccgaccaa actaacaaa actttggac gacctggtc 
atgttcatg gcacaagtg tcgcactgt taccccaag 
gaactcatc aactttccc caacagctg atcgatttg 
ttggaaaaa aatagcaca gttttaaac gccaccatg 
agaatgact ttttgccgg gctcttatc ctcctccgt 
aacaagaat ttgttagcc cccactgac ctactggaa 
ttatttttc aagcttctg aggtgccaa gacaaggcc 
ttgaggtcg ttcttggaa actcatatc atcacagat 
atcaagaac ttgaacgcg aaacataaa aatgccaag 
ttgaacaca accttacag aattttatg tttacaatg 
ctgaaggac actaattca agggctgcg aaaatgtca 
gtcgatatc atgattgaa ctttacaag aagaacgtt 
tggaatgat gccaaaaca gtgaacgtt attgcgtca 
ggttgtttc tcaaaattc acaaaagta atggtagct 
tccatgaag tttttccta gggaaggac tctgacgag 
aaagattca gactccgat tctgatgaa gaaatagac 
cccaaagaa gtgatgatg gccaacaaa gtcaacaaa 
aagacaaga aaaagggaa aaacagttg agtaaagta 
aaaaaattg gcatcaaag gcttacaag aagaaatca 
gcagcgcca gtttttaat ttttctgct ttacatttg 
atccatgac ccacaaggt atggcggaa aaactcttc 
aaccagatg gaaacaaca aataaacga tttgaaatc 
aaaatttta acactcgat gttatatca cggctcatt 
ggcttgcat cagttgttt ttgttcagt tattacccg 
tacattcag agatttctc caaccgcat cagcgtgaa 
gtgacaaga atattacaa tttgcggca caagcaagt 
catgagttg gtccctcca gacgttata gagcctgtt 
ttgaaaact ttagccaac aattttatt actgaaaga 
aattcctcg gatgttatg gctatagga ctgaatgca 
gtgcgtgag atatgctct aggtgtcct cttgcgatg 
aatgaagac ctactgcgt gacttggca atgtataaa 
aactacaga gatcgcgct gtcatgatg gccgcaaaa 
tctctcatc cacttattc agaaataca aggcctgag 
ttactgcat aagaaagac agggggcgc cctactgaa 
gcaactgaa gaacttggg tccataact tatggacaa 
gttgatgcc aaggattat gtaccaggg gctgaagtg 
cttttgaaa gaagatgac aaggaggtt gagattaat 
tcagaatct ggttctgat gatgaggaa tgggttgat 
attaaacac tcttctgat gaagaaggc gaaagtgaa 
gacaattgc agtgaagaa gaggaggaa aaagaagaa 
gaagaaaaa agcgaatca ccggaacgc aaaatattg 
aaagccaag tcttccaaa agagagctg gcgcacaaa 
gtcaccatc gaccgcatc ctaactgat gaagacttc 
aagaaaatt gacatggcc aacatcaga aaacaggtc 
cagggccct aagaaggga acaaagcgg aaaatcgaa 
gaagttaaa gtgagtggg gcgtccgaa cttgtcaaa 
ttgggtgat attgaaaac atatacaaa aaacgaaag 
catgataaa cagacaagg attgaaagt gttaaagcg 
ggccaagtg gatcgagag aagtttggc tacaaggac 
ggaagaatg aacattcac tgctcgaaa acaaaccgg 
gagaagagg aagacgaaa aactaccaa atggtcaaa 
cacaaaatg caaaggaag ggaaaacgc agcttcaaa 
gacaagcaa attgcgtta aggaatcac ttgttaaag 
ttgaaaaag atgaaataa
    mRNA sequence
    • >TC003433 RA
      tgaataaaa aaattcgtt cacgtaatt tatctacaa 
gtaaagttt ttagatttt gttatgttg gtgctagtg 
ttaggctcg ttttttagg ttaggtgtt ataacctca 
attgcacgt gttataaaa ttgcgaaaa tggtccgaa 
gacacaata accagctgc ctgaaaacc tcccccagc 
tgcaaaatc tcatcaaac gagaccccg cctcatacc 
atgaagaat tcacacaac agtatcaac atttccaaa 
acacaattc aagtttttg agttaagcc ccgaccaaa 
ctaacaaaa ctttggacg acctggtca tgttcatgg 
cacaagtgt cgcactgtt accccaagg aactcatca 
actttcccc aacagctga tcgatttgt tggaaaaaa 
atagcacag ttttaaacg ccaccatga gaatgactt 
tttgccggg ctcttatcc tcctccgta acaagaatt 
tgttagccc ccactgacc tactggaat tatttttca 
agcttctga ggtgccaag acaaggcct tgaggtcgt 
tcttggaaa ctcatatca tcacagata tcaagaact 
tgaacgcga aacataaaa atgccaagt tgaacacaa 
ccttacaga attttatgt ttacaatgc tgaaggaca 
ctaattcaa gggctgcga aaatgtcag tcgatatca 
tgattgaac tttacaaga agaacgttt ggaatgatg 
ccaaaacag tgaacgtta ttgcgtcag gttgtttct 
caaaattca caaaagtaa tggtagctt ccatgaagt 
ttttcctag ggaaggact ctgacgaga aagattcag 
actccgatt ctgatgaag aaatagacc ccaaagaag 
tgatgatgg ccaacaaag tcaacaaaa agacaagaa 
aaagggaaa aacagttga gtaaagtaa aaaaattgg 
catcaaagg cttacaaga agaaatcag cagcgccag 
tttttaatt tttctgctt tacatttga tccatgacc 
cacaaggta tggcggaaa aactcttca accagatgg 
aaacaacaa ataaacgat ttgaaatca aaattttaa 
cactcgatg ttatatcac ggctcattg gcttgcatc 
agttgtttt tgttcagtt attacccgt acattcaga 
gatttctcc aaccgcatc agcgtgaag tgacaagaa 
tattacaat ttgcggcac aagcaagtc atgagttgg 
tccctccag acgttatag agcctgttt tgaaaactt 
tagccaaca attttatta ctgaaagaa attcctcgg 
atgttatgg ctataggac tgaatgcag tgcgtgaga 
tatgctcta ggtgtcctc ttgcgatga atgaagacc 
tactgcgtg acttggcaa tgtataaaa actacagag 
atcgcgctg tcatgatgg ccgcaaaat ctctcatcc 
acttattca gaaatacaa ggcctgagt tactgcata 
agaaagaca gggggcgcc ctactgaag caactgaag 
aacttgggt ccataactt atggacaag ttgatgcca 
aggattatg taccagggg ctgaagtgc ttttgaaag 
aagatgaca aggaggttg agattaatt cagaatctg 
gttctgatg atgaggaat gggttgata ttaaacact 
cttctgatg aagaaggcg aaagtgaag acaattgca 
gtgaagaag aggaggaaa aagaagaag aagaaaaaa 
gcgaatcac cggaacgca aaatattga aagccaagt 
cttccaaaa gagagctgg cgcacaaag tcaccatcg 
accgcatcc taactgatg aagacttca agaaaattg 
acatggcca acatcagaa aacaggtcc agggcccta 
agaagggaa caaagcgga aaatcgaag aagttaaag 
tgagtgggg cgtccgaac ttgtcaaat tgggtgata 
ttgaaaaca tatacaaaa aacgaaagc atgataaac 
agacaagga ttgaaagtg ttaaagcgg gccaagtgg 
atcgagaga agtttggct acaaggacg gaagaatga 
acattcact gctcgaaaa caaaccggg agaagagga 
agacgaaaa actaccaaa tggtcaaac acaaaatgc 
aaaggaagg gaaaacgca gcttcaaag acaagcaaa 
ttgcgttaa ggaatcact tgttaaagt tgaaaaaga 
tgaaataat tttgtattt atcgttttg ttatttaat 
aaatgaaaa aaatcact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY