| General information | |||
| TC003433 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5146157..5148503 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003433 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03556 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
remarks: later than schedule,
- >TC003433 RA
MVRRHNNQL PENLPQLQN LIKRDPASY HEEFTQQYQ HFQNTIQVF ELSPDQTNK TLDDLVMFM AQVSHCYPK ELINFPQQL IDLLEKNST VLNATMRMT FCRALILLR NKNLLAPTD LLELFFKLL RCQDKALRS FLETHIITD IKNLNAKHK NAKLNTTLQ NFMFTMLKD TNSRAAKMS VDIMIELYK KNVWNDAKT VNVIASGCF SKFTKVMVA SMKFFLGKD SDEKDSDSD SDEEIDPKE VMMANKVNK KTRKREKQL SKVKKLASK AYKKKSAAP VFNFSALHL IHDPQGMAE KLFNQMETT NKRFEIKIL TLDVISRLI GLHQLFLFS YYPYIQRFL QPHQREVTR ILQFAAQAS HELVPPDVI EPVLKTLAN NFITERNSS DVMAIGLNA VREICSRCP LAMNEDLLR DLAMYKNYR DRAVMMAAK SLIHLFRNT RPELLHKKD RGRPTEATE ELGSITYGQ VDAKDYVPG AEVLLKEDD KEVEINSES GSDDEEWVD IKHSSDEEG ESEDNCSEE EEEKEEEEK SESPERKIL KAKSSKREL AHKVTIDRI LTDEDFKKI DMANIRKQV QGPKKGTKR KIEEVKVSG ASELVKLGD IENIYKKRK HDKQTRIES VKAGQVDRE KFGYKDGRM NIHCSKTNR EKRKTKNYQ MVKHKMQRK GKRSFKDKQ IALRNHLLK LKKMK
- >TC003433 RA
atggtccga agacacaat aaccagctg cctgaaaac ctcccccag ctgcaaaat ctcatcaaa cgagacccc gcctcatac catgaagaa ttcacacaa cagtatcaa catttccaa aacacaatt caagttttt gagttaagc cccgaccaa actaacaaa actttggac gacctggtc atgttcatg gcacaagtg tcgcactgt taccccaag gaactcatc aactttccc caacagctg atcgatttg ttggaaaaa aatagcaca gttttaaac gccaccatg agaatgact ttttgccgg gctcttatc ctcctccgt aacaagaat ttgttagcc cccactgac ctactggaa ttatttttc aagcttctg aggtgccaa gacaaggcc ttgaggtcg ttcttggaa actcatatc atcacagat atcaagaac ttgaacgcg aaacataaa aatgccaag ttgaacaca accttacag aattttatg tttacaatg ctgaaggac actaattca agggctgcg aaaatgtca gtcgatatc atgattgaa ctttacaag aagaacgtt tggaatgat gccaaaaca gtgaacgtt attgcgtca ggttgtttc tcaaaattc acaaaagta atggtagct tccatgaag tttttccta gggaaggac tctgacgag aaagattca gactccgat tctgatgaa gaaatagac cccaaagaa gtgatgatg gccaacaaa gtcaacaaa aagacaaga aaaagggaa aaacagttg agtaaagta aaaaaattg gcatcaaag gcttacaag aagaaatca gcagcgcca gtttttaat ttttctgct ttacatttg atccatgac ccacaaggt atggcggaa aaactcttc aaccagatg gaaacaaca aataaacga tttgaaatc aaaatttta acactcgat gttatatca cggctcatt ggcttgcat cagttgttt ttgttcagt tattacccg tacattcag agatttctc caaccgcat cagcgtgaa gtgacaaga atattacaa tttgcggca caagcaagt catgagttg gtccctcca gacgttata gagcctgtt ttgaaaact ttagccaac aattttatt actgaaaga aattcctcg gatgttatg gctatagga ctgaatgca gtgcgtgag atatgctct aggtgtcct cttgcgatg aatgaagac ctactgcgt gacttggca atgtataaa aactacaga gatcgcgct gtcatgatg gccgcaaaa tctctcatc cacttattc agaaataca aggcctgag ttactgcat aagaaagac agggggcgc cctactgaa gcaactgaa gaacttggg tccataact tatggacaa gttgatgcc aaggattat gtaccaggg gctgaagtg cttttgaaa gaagatgac aaggaggtt gagattaat tcagaatct ggttctgat gatgaggaa tgggttgat attaaacac tcttctgat gaagaaggc gaaagtgaa gacaattgc agtgaagaa gaggaggaa aaagaagaa gaagaaaaa agcgaatca ccggaacgc aaaatattg aaagccaag tcttccaaa agagagctg gcgcacaaa gtcaccatc gaccgcatc ctaactgat gaagacttc aagaaaatt gacatggcc aacatcaga aaacaggtc cagggccct aagaaggga acaaagcgg aaaatcgaa gaagttaaa gtgagtggg gcgtccgaa cttgtcaaa ttgggtgat attgaaaac atatacaaa aaacgaaag catgataaa cagacaagg attgaaagt gttaaagcg ggccaagtg gatcgagag aagtttggc tacaaggac ggaagaatg aacattcac tgctcgaaa acaaaccgg gagaagagg aagacgaaa aactaccaa atggtcaaa cacaaaatg caaaggaag ggaaaacgc agcttcaaa gacaagcaa attgcgtta aggaatcac ttgttaaag ttgaaaaag atgaaataa
- >TC003433 RA
tgaataaaa aaattcgtt cacgtaatt tatctacaa gtaaagttt ttagatttt gttatgttg gtgctagtg ttaggctcg ttttttagg ttaggtgtt ataacctca attgcacgt gttataaaa ttgcgaaaa tggtccgaa gacacaata accagctgc ctgaaaacc tcccccagc tgcaaaatc tcatcaaac gagaccccg cctcatacc atgaagaat tcacacaac agtatcaac atttccaaa acacaattc aagtttttg agttaagcc ccgaccaaa ctaacaaaa ctttggacg acctggtca tgttcatgg cacaagtgt cgcactgtt accccaagg aactcatca actttcccc aacagctga tcgatttgt tggaaaaaa atagcacag ttttaaacg ccaccatga gaatgactt tttgccggg ctcttatcc tcctccgta acaagaatt tgttagccc ccactgacc tactggaat tatttttca agcttctga ggtgccaag acaaggcct tgaggtcgt tcttggaaa ctcatatca tcacagata tcaagaact tgaacgcga aacataaaa atgccaagt tgaacacaa ccttacaga attttatgt ttacaatgc tgaaggaca ctaattcaa gggctgcga aaatgtcag tcgatatca tgattgaac tttacaaga agaacgttt ggaatgatg ccaaaacag tgaacgtta ttgcgtcag gttgtttct caaaattca caaaagtaa tggtagctt ccatgaagt ttttcctag ggaaggact ctgacgaga aagattcag actccgatt ctgatgaag aaatagacc ccaaagaag tgatgatgg ccaacaaag tcaacaaaa agacaagaa aaagggaaa aacagttga gtaaagtaa aaaaattgg catcaaagg cttacaaga agaaatcag cagcgccag tttttaatt tttctgctt tacatttga tccatgacc cacaaggta tggcggaaa aactcttca accagatgg aaacaacaa ataaacgat ttgaaatca aaattttaa cactcgatg ttatatcac ggctcattg gcttgcatc agttgtttt tgttcagtt attacccgt acattcaga gatttctcc aaccgcatc agcgtgaag tgacaagaa tattacaat ttgcggcac aagcaagtc atgagttgg tccctccag acgttatag agcctgttt tgaaaactt tagccaaca attttatta ctgaaagaa attcctcgg atgttatgg ctataggac tgaatgcag tgcgtgaga tatgctcta ggtgtcctc ttgcgatga atgaagacc tactgcgtg acttggcaa tgtataaaa actacagag atcgcgctg tcatgatgg ccgcaaaat ctctcatcc acttattca gaaatacaa ggcctgagt tactgcata agaaagaca gggggcgcc ctactgaag caactgaag aacttgggt ccataactt atggacaag ttgatgcca aggattatg taccagggg ctgaagtgc ttttgaaag aagatgaca aggaggttg agattaatt cagaatctg gttctgatg atgaggaat gggttgata ttaaacact cttctgatg aagaaggcg aaagtgaag acaattgca gtgaagaag aggaggaaa aagaagaag aagaaaaaa gcgaatcac cggaacgca aaatattga aagccaagt cttccaaaa gagagctgg cgcacaaag tcaccatcg accgcatcc taactgatg aagacttca agaaaattg acatggcca acatcagaa aacaggtcc agggcccta agaagggaa caaagcgga aaatcgaag aagttaaag tgagtgggg cgtccgaac ttgtcaaat tgggtgata ttgaaaaca tatacaaaa aacgaaagc atgataaac agacaagga ttgaaagtg ttaaagcgg gccaagtgg atcgagaga agtttggct acaaggacg gaagaatga acattcact gctcgaaaa caaaccggg agaagagga agacgaaaa actaccaaa tggtcaaac acaaaatgc aaaggaagg gaaaacgca gcttcaaag acaagcaaa ttgcgttaa ggaatcact tgttaaagt tgaaaaaga tgaaataat tttgtattt atcgttttg ttatttaat aaatgaaaa aaatcact
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
