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Gene information
General information
TC003429 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 395666..436538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003429
    iBeetle screen iB_03555
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellarium not present - Number of animals showing the phenotype: 4
    tropharium shortened - Number of animals showing the phenotype: 4
    tropharium rounded - Number of animals showing the phenotype: 4
    egg chamber not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Comment: died with pupal and adult features

    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    Pupal morphology
    eyes size decreased - Number of animals showing the phenotype: 8

    antenna size shortened - Number of animals showing the phenotype: 8

    head bristle mostlynot present - Number of animals showing the phenotype: 8
    labrum size decreased - Number of animals showing the phenotype: 8
    labial palp broader - Number of animals showing the phenotype: 8

    Comment: labial palps distance broader
    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 8
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 8

    oblique dorsal thoracic muscles size shortened - Number of animals showing the phenotype: 8

    abdomen gin-traps not present - Number of animals showing the phenotype: 8

    abdomen gin-trap setae not present - Number of animals showing the phenotype: 8
    female genital lobes size decreased - Number of animals showing the phenotype: 8

    Adult morphology
    funicle number decreased - Number of animals showing the phenotype: 8


    club fused - Number of animals showing the phenotype: 8
    Comment: the 3 segments of the club seems to be fused

    antenna shortened - Number of animals showing the phenotype: 8


    eyes size decreased - Number of animals showing the phenotype: 6


    legs tibia thinner - Number of animals showing the phenotype: 6


    legs pretarsus decreased - Number of animals showing the phenotype: 6

    Comment: no pretarsal claws

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003429 RA
      MWPAWGHPA EAAAAASPL PLVLRCLAQ ALATGFHRF 
      LARFSGRRR SLYRYNPKY PPPWAFLVL VLASSLFSI 
      VDACSSRTT PKPRPPAPT ARPNITFHT YECPPAYAA 
      WYCLNGATC FTVKIGDSL LYNCECAEG YMGPRCEYK 
      DLDGSYLPS QRRFMLETA SIAGGATIA VFLVVIVCV 
      VVYLQYKRY NKMSRASTD CVDGHSTPL NTPTFGTRW 
      RTSPPADIP VAVQEAGDS KEVSWNVIR SQRYTLPIN 
      RDVCSA
    CDS sequence
    • >TC003429 RA
      atgtggccg gcatggggc catcccgcc gaggcggcg 
      gcggcggcg agccccctg cctctcgtc ctccggtgc 
      ctggcgcaa gcactcgcc accggcttc caccgcttc 
      ctagcaaga ttttcaggt cgaagacga tcactatat 
      cgatacaat ccgaaatat ccgccacct tgggccttc 
      ctagtttta gtgttagcg tcttcttta tttagtata 
      gtcgatgcg tgttcgtca cgcaccacg ccgaaaccg 
      aggcctcca gcacctact gcacggcct aacatcaca 
      ttccacacg tacgaatgt ccgccggcg tacgcggcc 
      tggtactgc cttaacgga gcaacgtgc tttaccgtc 
      aaaattggc gattccctc ctatacaat tgcgagtgt 
      gcagaaggt tacatgggt cctagatgt gaatataaa 
      gatttagac ggttcatat ttaccgtca caacgaagg 
      ttcatgttg gaaacggcg agtatagcc ggtggggcg 
      acgatagcc gttttttta gtggtgata gtgtgcgtc 
      gttgtgtat ctgcagtat aaacgctat aataaaatg 
      agtcgtgcg agcacggac tgcgtggac ggacacagc 
      acccctttg aacacgccg accttcggc acccgatgg 
      cggacgagt ccgccggcg gatatcccg gtggcggtg 
      caagaagcg ggcgactcg aaggaggtc agttggaac 
      gtgatcagg tcgcagcga tacacgctt ccaatcaat 
      cgcgacgtg tgcagtgca tag
    mRNA sequence
    • >TC003429 RA
      tgggttttc gcaaatcga atattaatt atttgctcc 
      gtttttaat tggctgacc gcgaatttt accgattcg 
      accccacgc aacctgtga acccgctcc gatatatcg 
      tcgaccgat ttcattttt tttcgttgc agtgttgtg 
      ttttgtgtg gtaattttt tatttcgtt atggttttt 
      tttgcggcc gcgatggct tgtgatgtc tgaaaactt 
      gtggaaaca ctgcgagcc ggattttcc gctgtgtga 
      tgtttttcg caaacagac cgagtgcta tggaatttt 
      tatttaatg tgagcttta ttggaccac cttcatgtt 
      gctgagatc ttttcaatt ggcgcacgt gatgtggcc 
      ggcatgggg ccatcccgc cgaggcggc ggcggcggc 
      gagccccct gcctctcgt cctccggtg cctggcgca 
      agcactcgc caccggctt ccaccgctt cctagcaag 
      attttcagg tcgaagacg atcactata tcgatacaa 
      tccgaaata tccgccacc ttgggcctt cctagtttt 
      agtgttagc gtcttcttt atttagtat agtcgatgc 
      gtgttcgtc acgcaccac gccgaaacc gaggcctcc 
      agcacctac tgcacggcc taacatcac attccacac 
      gtacgaatg tccgccggc gtacgcggc ctggtactg 
      ccttaacgg agcaacgtg ctttaccgt caaaattgg 
      cgattccct cctatacaa ttgcgagtg tgcagaagg 
      ttacatggg tcctagatg tgaatataa agatttaga 
      cggttcata tttaccgtc acaacgaag gttcatgtt 
      ggaaacggc gagtatagc cggtggggc gacgatagc 
      cgttttttt agtggtgat agtgtgcgt cgttgtgta 
      tctgcagta taaacgcta taataaaat gagtcgtgc 
      gagcacgga ctgcgtgga cggacacag caccccttt 
      gaacacgcc gaccttcgg cacccgatg gcggacgag 
      tccgccggc ggatatccc ggtggcggt gcaagaagc 
      gggcgactc gaaggaggt cagttggaa cgtgatcag 
      gtcgcagcg atacacgct tccaatcaa tcgcgacgt 
      gtgcagtgc atagctagc aaactgcta ttcaaatta 
      acagtgatg tcggcttta atgtgttat agaatcaaa 
      ctatgcctt tgtaaacca acaaataca gaagtgagt 
      agcacttgt tatacgcag ctttcgttc acaccgtgc 
      agcttattg tcaatcctt tggcagaac ctacagttt 
      ggagtaatg ttgtcaaac attttgcga aattcctac 
      gtccgaatg cgacttttt ggattgttc cgattttgc 
      tagctatga cttggcgtt ctcagtgag cagaactgt 
      gtcacaaca gtgtatttg tacagcaag tctgatata 
      tgtaagtct gaactttaa gttattaag ttatttaaa 
      tgaattata tgtaaatag gtacctcgc cgaggtatt 
      ttaattaaa ttgtataat acgcagtta cgtatgttt 
      tcgaactat tcgtgcaga ttgagtggt tttaaatta 
      ttatccttt ttagtttgt tgaaatatt acattcccg 
      gatgtatcg tttattcaa acctcgcta acaaacgtt 
      gttattgtt aaattgttg atttaataa gtgattttt 
      atagctgat ttattgata ggcgtgcaa gtatgttga 
      tgggattga gcagtgatt agtcgaatg tagcatagc 
      aataagtac ctacaccaa acgtacatc aaatggata 
      tttttcgta aatttttac gtacacgct atcaataac 
      tacaacacc gatgaacga acgaacttg ttgtataaa 
      tgtagatat acagtaact tattccaat aacggatgc 
      tcgcattcg caattattg gcaataata tgagatgta 
      atttagtaa tttaaatat ttattgtaa gcacttggt 
      tcctaactt attgaagta aacttttgt attatttta 
      gagacgcgt tcccatacc aaaactaca aatatcctt 
      aacccaatg tattgtttt aaattatcc aagtattct 
      aacatgatg tgtacctac ttagcaagc tccgccgat 
      atttgtgat tttggtatc aggccaaat acgtttgcc 
      agttgttta gccgtaaat gattattta cgcccccaa 
      accaaagcc caagtgtat cttttgtgc agcccaaac 
      gacacaatc gcattacca tttcactcc atgatatta 
      tttatgctt tctgatacg ttttgtttg ggctccaca 
      aaatatatc gaacaagta ttaatatca ttaatatat 
      taatagtta tatt