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Gene information
General information
TC003404 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid15: 135962..141474
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003404
    iBeetle screen iB_03550
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    legs tarsus partially necrotic - Number of animals showing the phenotype: 8


    legs tarsus distal cuticularisation lighter - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003404 RA
      MWKTLSILL LCLLLEPSW SRLAEDITY SSLDNCVTD 
      HCFHQPAEA CPSPDSKCR CRKLPYCKS AAVCCNVNK 
      FQLTEGLAC ANISGNGHV ESLHIRNAS LDILNVEDW 
      RKLKYLRYM TITDGQINS VVGEFAKHT IVSCLNLSS 
      NGIVNFENR SLVNLYNLS FLDLSHNNL SDVPRFKKD 
      GNITLDISA NQHMLCSNL FDALKRTEL HFSNINNSF 
      CLFARTFHW FNTTETVPL TQVIGTHKL RKDCRKNCT 
      CEPSRLDLV SGKQATIAV AVNCSGQSW TSLPSPLPP 
      NTIALNVSN NNITSLELF STDSSYQNI RELYADNNQ 
      ITSILPLEG SQFLSSFAV LSLRNNKLK SLPTYIFSN 
      ILDRNNYVR YVFLGLNRL QCDCNTAKV LKVWLLTKQ 
      KNIPDFEEI YCDNMDMKV IDLNPSKLC QTQQDWTDY 
      IYYIITIEV VLLVGLIAK VVYDYCVFK TSGYLPWPA 
      SKMPKLPCD WLCE
    CDS sequence
    • >TC003404 RA
      atgtggaag acactaagc attttgctg ctctgtctt 
      ctgctcgaa ccctcatgg agtcgctta gccgaagac 
      atcacctac tcatccctg gacaactgc gtaaccgac 
      cactgtttc catcaacca gcagaggca tgccctagc 
      cctgacagc aagtgccgt tgtcgcaaa ctcccatac 
      tgcaaaagt gcggccgtt tgctgcaac gttaataaa 
      ttccaacta acggaaggc ttggcatgc gcaaacatc 
      agtggcaac ggtcatgtg gagtcgctg cacatacgg 
      aacgccagt ctggacatc ctaaacgtg gaggactgg 
      aggaaactc aaatatctg agatacatg accataaca 
      gacggccaa ataaacagc gtagtcggg gaattcgcc 
      aaacacacc atagtctcc tgtttgaat ttatcctca 
      aacgggatc gtcaatttt gaaaataga tctctggtc 
      aatttgtac aatctgagc ttcttggat ctttcacac 
      aacaattta tcggacgtc cctcggttc aaaaaagat 
      gggaatata actctagac atatcagct aatcagcac 
      atgttatgc tcaaaccta ttcgacgct ttaaagcgc 
      accgaactc cacttcagc aacataaac aattccttt 
      tgtctattt gcccgaacc tttcactgg ttcaatacg 
      actgaaact gtccctctt acacaagtg attggaaca 
      cataagttg cgtaaagat tgccgcaaa aattgcact 
      tgtgagccc tcaagactg gacctagtg tcaggaaaa 
      caagcaacg attgcagtg gctgttaac tgctccgga 
      cagagctgg acatcactg ccttcacct ctgccaccc 
      aacactata gctctgaac gtttcgaat aataacata 
      acttcgctg gaactgttc agtaccgac tcctcctac 
      cagaatatt agggagctg tatgctgat aataaccag 
      attacgtcc attctcccg ctggagggg tcgcagttt 
      ttgagcagt ttcgcagtc ctgagtctt agaaacaac 
      aagttgaaa tcgctccca acttacata ttctcgaac 
      atactggac cggaataat tacgtccgg tacgttttt 
      ctcggtctg aacagactc cagtgtgat tgtaacacg 
      gccaaagtt ttgaaggtg tggctgttg acgaagcag 
      aaaaacatc cccgatttc gaagaaatc tactgtgat 
      aacatggat atgaaagtg atagatttg aacccttcc 
      aagctctgt cagacgcaa caagactgg actgactac 
      atctactac atcatcacc atagaggtg gtgcttctc 
      gtgggtctg attgccaaa gtcgtttac gattactgt 
      gtttttaaa acctccggt tatttgccg tggccggcg 
      agcaaaatg ccaaagtta ccatgcgat tggttatgt 
      gaatag
    mRNA sequence
    • >TC003404 RA
      taaaagtag agtgtgagg aaatattca aaatatttg 
      attttcctt gaatggggt cttttgaaa tggagtaat 
      ggaagtttg tttgaggtg tgggcagcg agtttcatt 
      gatccaatt actgaataa agtttaatt aacaataat 
      aattgggag taaagagtg gagaacaaa tagatggcg 
      ccttgatat caacattag tagtttact atatttatc 
      tgttattat gttcatttt atgttcacg tttccgtgt 
      gcctcatca aggccgtac caaggccat agcaaaagc 
      tatagtagt gtgttatgt ttaggttaa ttctgttaa 
      aaacatctc gtcccatag tgtctttgt gtaactttg 
      tagtaaagg cttaaaaca gttctatgg ccaaagaat 
      aatgtgtac cgttgattg tgttcctct acctgagtg 
      aaaagatgt ggaagacac taagcattt tgctgctct 
      gtcttctgc tcgaaccct catggagtc gcttagccg 
      aagacatca cctactcat ccctggaca actgcgtaa 
      ccgaccact gtttccatc aaccagcag aggcatgcc 
      ctagccctg acagcaagt gccgttgtc gcaaactcc 
      catactgca aaagtgcgg ccgtttgct gcaacgtta 
      ataaattcc aactaacgg aaggcttgg catgcgcaa 
      acatcagtg gcaacggtc atgtggagt cgctgcaca 
      tacggaacg ccagtctgg acatcctaa acgtggagg 
      actggagga aactcaaat atctgagat acatgacca 
      taacagacg gccaaataa acagcgtag tcggggaat 
      tcgccaaac acaccatag tctcctgtt tgaatttat 
      cctcaaacg ggatcgtca attttgaaa atagatctc 
      tggtcaatt tgtacaatc tgagcttct tggatcttt 
      cacacaaca atttatcgg acgtccctc ggttcaaaa 
      aagatggga atataactc tagacatat cagctaatc 
      agcacatgt tatgctcaa acctattcg acgctttaa 
      agcgcaccg aactccact tcagcaaca taaacaatt 
      ccttttgtc tatttgccc gaacctttc actggttca 
      atacgactg aaactgtcc ctcttacac aagtgattg 
      gaacacata agttgcgta aagattgcc gcaaaaatt 
      gcacttgtg agccctcaa gactggacc tagtgtcag 
      gaaaacaag caacgattg cagtggctg ttaactgct 
      ccggacaga gctggacat cactgcctt cacctctgc 
      cacccaaca ctatagctc tgaacgttt cgaataata 
      acataactt cgctggaac tgttcagta ccgactcct 
      cctaccaga atattaggg agctgtatg ctgataata 
      accagatta cgtccattc tcccgctgg aggggtcgc 
      agtttttga gcagtttcg cagtcctga gtcttagaa 
      acaacaagt tgaaatcgc tcccaactt acatattct 
      cgaacatac tggaccgga ataattacg tccggtacg 
      tttttctcg gtctgaaca gactccagt gtgattgta 
      acacggcca aagttttga aggtgtggc tgttgacga 
      agcagaaaa acatccccg atttcgaag aaatctact 
      gtgataaca tggatatga aagtgatag atttgaacc 
      cttccaagc tctgtcaga cgcaacaag actggactg 
      actacatct actacatca tcaccatag aggtggtgc 
      ttctcgtgg gtctgattg ccaaagtcg tttacgatt 
      actgtgttt ttaaaacct ccggttatt tgccgtggc 
      cggcgagca aaatgccaa agttaccat gcgattggt 
      tatgtgaat agttttatt ttagtcgca acgatgtgc 
      ctgttaatg cttccgcct tgtatcatg gttgtgaaa 
      tgtttactg tagtaggga ttagtgtag gaagttttg 
      tgctctcaa tcaagtttg aaacgaaaa gtctgacac 
      accaacagt catattagt tttctagac tcaaatcta 
      atacgaaaa gacagtaca attgcggaa atttaaata 
      aattcgcga atttatttt aaattttct atcttattt 
      acaaattat gaactatgg ctttatatg tcttttact 
      gtaaactat ttttcttca ttaggttta tgcaacttt 
      agctccgta tgtgttttt atttattgt cttcttttg 
      aaaacactg tttttaaca tcactttca gaatattaa 
      tcctctaat atgttattc ccttttatt tgcttatgc 
      aaatatttt cctaataat gtgcaattg tatatattt 
      catttttta cattatcta cggtatgtc tttatttgt 
      gcaactctt ccctttata tttttgttg tttaattgc 
      tcaatttat agtagttgt ttgtgtttt ttgttttga 
      tatttattt tatttattt ttttccaaa ttgtggtaa 
      aagacaata tatcaagta taccaattg atttacaat 
      aattccggt gtgtattta ctatttgtt gtaaaatac 
      atgaaccgc ctaagtatg ggtagcaac aaaatgaac 
      tctgtaatc attaatact ttttggaat gtaatttaa 
      ttgtgttta ctgtaagca cgtccaaat atttaattg 
      tagtcgttt tacagtttt tatactatt aagtaatac 
      tcatccaag gacaaaatc aaaaatcgt ctacgaatt 
      atcaaaacg tgtcacagc tttttagtt tttacaaat 
      gtatggtca aaaattgtt tgaaatctc tttacccaa 
      aatttttat tttatttag catatttta cttacttta 
      agtacaata tcttgtttg ctaatgtat agataaata 
      ttgtttctt ttgtaatat tgtttcgta taataaagt 
      gtcttatca aac