General information | |||
TC003404 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 4331472..4336984 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003404 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03550 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
legs tarsus distal cuticularisation lighter - Number of animals showing the phenotype: 8
- >TC003404 RA
MWKTLSILL LCLLLEPSW SRLAEDITY SSLDNCVTD HCFHQPAEA CPSPDSKCR CRKLPYCKS AAVCCNVNK FQLTEGLAC ANISGNGHV ESLHIRNAS LDILNVEDW RKLKYLRYM TITDGQINS VVGEFAKHT IVSCLNLSS NGIVNFENR SLVNLYNLS FLDLSHNNL SDVPRFKKD GNITLDISA NQHMLCSNL FDALKRTEL HFSNINNSF CLFARTFHW FNTTETVPL TQVIGTHKL RKDCRKNCT CEPSRLDLV SGKQATIAV AVNCSGQSW TSLPSPLPP NTIALNVSN NNITSLELF STDSSYQNI RELYADNNQ ITSILPLEG SQFLSSFAV LSLRNNKLK SLPTYIFSN ILDRNNYVR YVFLGLNRL QCDCNTAKV LKVWLLTKQ KNIPDFEEI YCDNMDMKV IDLNPSKLC QTQQDWTDY IYYIITIEV VLLVGLIAK VVYDYCVFK TSGYLPWPA SKMPKLPCD WLCE
- >TC003404 RA
atgtggaag acactaagc attttgctg ctctgtctt ctgctcgaa ccctcatgg agtcgctta gccgaagac atcacctac tcatccctg gacaactgc gtaaccgac cactgtttc catcaacca gcagaggca tgccctagc cctgacagc aagtgccgt tgtcgcaaa ctcccatac tgcaaaagt gcggccgtt tgctgcaac gttaataaa ttccaacta acggaaggc ttggcatgc gcaaacatc agtggcaac ggtcatgtg gagtcgctg cacatacgg aacgccagt ctggacatc ctaaacgtg gaggactgg aggaaactc aaatatctg agatacatg accataaca gacggccaa ataaacagc gtagtcggg gaattcgcc aaacacacc atagtctcc tgtttgaat ttatcctca aacgggatc gtcaatttt gaaaataga tctctggtc aatttgtac aatctgagc ttcttggat ctttcacac aacaattta tcggacgtc cctcggttc aaaaaagat gggaatata actctagac atatcagct aatcagcac atgttatgc tcaaaccta ttcgacgct ttaaagcgc accgaactc cacttcagc aacataaac aattccttt tgtctattt gcccgaacc tttcactgg ttcaatacg actgaaact gtccctctt acacaagtg attggaaca cataagttg cgtaaagat tgccgcaaa aattgcact tgtgagccc tcaagactg gacctagtg tcaggaaaa caagcaacg attgcagtg gctgttaac tgctccgga cagagctgg acatcactg ccttcacct ctgccaccc aacactata gctctgaac gtttcgaat aataacata acttcgctg gaactgttc agtaccgac tcctcctac cagaatatt agggagctg tatgctgat aataaccag attacgtcc attctcccg ctggagggg tcgcagttt ttgagcagt ttcgcagtc ctgagtctt agaaacaac aagttgaaa tcgctccca acttacata ttctcgaac atactggac cggaataat tacgtccgg tacgttttt ctcggtctg aacagactc cagtgtgat tgtaacacg gccaaagtt ttgaaggtg tggctgttg acgaagcag aaaaacatc cccgatttc gaagaaatc tactgtgat aacatggat atgaaagtg atagatttg aacccttcc aagctctgt cagacgcaa caagactgg actgactac atctactac atcatcacc atagaggtg gtgcttctc gtgggtctg attgccaaa gtcgtttac gattactgt gtttttaaa acctccggt tatttgccg tggccggcg agcaaaatg ccaaagtta ccatgcgat tggttatgt gaatag
- >TC003404 RA
taaaagtag agtgtgagg aaatattca aaatatttg attttcctt gaatggggt cttttgaaa tggagtaat ggaagtttg tttgaggtg tgggcagcg agtttcatt gatccaatt actgaataa agtttaatt aacaataat aattgggag taaagagtg gagaacaaa tagatggcg ccttgatat caacattag tagtttact atatttatc tgttattat gttcatttt atgttcacg tttccgtgt gcctcatca aggccgtac caaggccat agcaaaagc tatagtagt gtgttatgt ttaggttaa ttctgttaa aaacatctc gtcccatag tgtctttgt gtaactttg tagtaaagg cttaaaaca gttctatgg ccaaagaat aatgtgtac cgttgattg tgttcctct acctgagtg aaaagatgt ggaagacac taagcattt tgctgctct gtcttctgc tcgaaccct catggagtc gcttagccg aagacatca cctactcat ccctggaca actgcgtaa ccgaccact gtttccatc aaccagcag aggcatgcc ctagccctg acagcaagt gccgttgtc gcaaactcc catactgca aaagtgcgg ccgtttgct gcaacgtta ataaattcc aactaacgg aaggcttgg catgcgcaa acatcagtg gcaacggtc atgtggagt cgctgcaca tacggaacg ccagtctgg acatcctaa acgtggagg actggagga aactcaaat atctgagat acatgacca taacagacg gccaaataa acagcgtag tcggggaat tcgccaaac acaccatag tctcctgtt tgaatttat cctcaaacg ggatcgtca attttgaaa atagatctc tggtcaatt tgtacaatc tgagcttct tggatcttt cacacaaca atttatcgg acgtccctc ggttcaaaa aagatggga atataactc tagacatat cagctaatc agcacatgt tatgctcaa acctattcg acgctttaa agcgcaccg aactccact tcagcaaca taaacaatt ccttttgtc tatttgccc gaacctttc actggttca atacgactg aaactgtcc ctcttacac aagtgattg gaacacata agttgcgta aagattgcc gcaaaaatt gcacttgtg agccctcaa gactggacc tagtgtcag gaaaacaag caacgattg cagtggctg ttaactgct ccggacaga gctggacat cactgcctt cacctctgc cacccaaca ctatagctc tgaacgttt cgaataata acataactt cgctggaac tgttcagta ccgactcct cctaccaga atattaggg agctgtatg ctgataata accagatta cgtccattc tcccgctgg aggggtcgc agtttttga gcagtttcg cagtcctga gtcttagaa acaacaagt tgaaatcgc tcccaactt acatattct cgaacatac tggaccgga ataattacg tccggtacg tttttctcg gtctgaaca gactccagt gtgattgta acacggcca aagttttga aggtgtggc tgttgacga agcagaaaa acatccccg atttcgaag aaatctact gtgataaca tggatatga aagtgatag atttgaacc cttccaagc tctgtcaga cgcaacaag actggactg actacatct actacatca tcaccatag aggtggtgc ttctcgtgg gtctgattg ccaaagtcg tttacgatt actgtgttt ttaaaacct ccggttatt tgccgtggc cggcgagca aaatgccaa agttaccat gcgattggt tatgtgaat agttttatt ttagtcgca acgatgtgc ctgttaatg cttccgcct tgtatcatg gttgtgaaa tgtttactg tagtaggga ttagtgtag gaagttttg tgctctcaa tcaagtttg aaacgaaaa gtctgacac accaacagt catattagt tttctagac tcaaatcta atacgaaaa gacagtaca attgcggaa atttaaata aattcgcga atttatttt aaattttct atcttattt acaaattat gaactatgg ctttatatg tcttttact gtaaactat ttttcttca ttaggttta tgcaacttt agctccgta tgtgttttt atttattgt cttcttttg aaaacactg tttttaaca tcactttca gaatattaa tcctctaat atgttattc ccttttatt tgcttatgc aaatatttt cctaataat gtgcaattg tatatattt catttttta cattatcta cggtatgtc tttatttgt gcaactctt ccctttata tttttgttg tttaattgc tcaatttat agtagttgt ttgtgtttt ttgttttga tatttattt tatttattt ttttccaaa ttgtggtaa aagacaata tatcaagta taccaattg atttacaat aattccggt gtgtattta ctatttgtt gtaaaatac atgaaccgc ctaagtatg ggtagcaac aaaatgaac tctgtaatc attaatact ttttggaat gtaatttaa ttgtgttta ctgtaagca cgtccaaat atttaattg tagtcgttt tacagtttt tatactatt aagtaatac tcatccaag gacaaaatc aaaaatcgt ctacgaatt atcaaaacg tgtcacagc tttttagtt tttacaaat gtatggtca aaaattgtt tgaaatctc tttacccaa aatttttat tttatttag catatttta cttacttta agtacaata tcttgtttg ctaatgtat agataaata ttgtttctt ttgtaatat tgtttcgta taataaagt gtcttatca aac
