Status
Please wait ...
Gene information
General information
TC003401 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4303152..4306973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003401
    iBeetle screen iB_03549

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003401 RA
      MELLTPHLS SVRVPTATS KVYKDECVF SFDNPETET 
      GLYVGLKSF FGLGRDYVE KHFHKTGEA VYLHIRRVR 
      HEVSSPPQG DGPEKKITR LAIGVDGGF DPEAMSKKF 
      EFEDSYNIV VLPGFSTIP WPNTELPQI VKSSIEGIL 
      EAPSASKVA EMEALTGTW DGEARVVSQ FAHNLPQLD 
      NGKKIPPSG WQCEKCDKT DNLWLNLTD GSILCGRRF 
      YDGSGGNNH AIEHYNATG HPLAVKLGT ITKEGKGDV 
      FSYKEDDMV EDPNLVQHL AHWGINIAN MEKTEKSMV 
      ELELELNQK SNEWSALQE SDGQLKPIY GPGYTGMTN 
      MGNSCYLNS VMQMVFSIP DFIQRYYHD AELFFNNVV 
      GDPCEDFNA QMAKLAVGL HSGKYSVPP PAGSPVDAD 
      PPGICPLMF KALVGRGHP EFSTKKQQD AQEFFLHIL 
      TLLERNSKN KVNPGDCFK FKVEEKFQC SASKKVKYL 
      TRSDVLLPL IIPMDAAIN KDEVAAYET KKLEAEKTG 
      KKLLANTIV RPKINFLSC LELFTQSEV INNFYSSAV 
      NANVTARKT TRLATFPDY LCIQLKKFM LREDWVPIK 
      LDVSIEMPD ILDISSLRG TGPQSDEEL LPEPKVQPP 
      APVMDEGVL AQLADMGFP PEACKRAVF FTHNSGLEA 
      ATAWIMEHI TDSDFSDPF VPPGTESSH FTPNAEALA 
      SIVAMGFTQ HQATKALKA TDNNVERAM DWIFSHQDE 
      LDNVTSPPP PEFRDGDGR YKLVAFVSH MGTSTMVGH 
      YVVHILKNG QWVIFNDNK VALSERPPK DLGYMYLYE 
      RL
    CDS sequence
    • >TC003401 RA
      atggaatta cttacaccg catttaagc agcgttaga 
      gtcccaaca gcgaccagc aaagtatac aaggacgaa 
      tgtgtcttc tctttcgat aatccggaa accgagacc 
      ggtctgtac gtaggtctg aagagtttt ttcggactt 
      gggcgcgat tatgttgaa aaacacttc cacaaaacg 
      ggagaagcc gtctatctc cacatccgg cgagtgcgt 
      catgaagtc tcatcaccc ccacagggc gatggaccc 
      gagaagaaa atcacgcgc ttggccatt ggagttgac 
      ggtggtttt gatccagag gccatgtca aagaagttt 
      gagtttgag gactcctac aatattgtc gttttgcca 
      ggtttcagc accatcccc tggcccaac acagagctt 
      ccccaaatt gtaaaatcg tctattgag ggcattttg 
      gaagccccc tcggccagt aaagtcgcc gaaatggag 
      gctttgacg gggacgtgg gatggcgaa gctcgggtt 
      gtctcccag tttgcccac aacttgcca cagttagat 
      aatgggaaa aaaattcca ccttctggg tggcagtgc 
      gaaaaatgt gataaaact gataatctg tggctaaac 
      ttaaccgat gggtctatc ttatgtggc aggaggttt 
      tatgatgga agtggagga aataatcac gccattgaa 
      cattataac gccaccggt catccctta gctgttaaa 
      ttggggacg atcacaaag gaaggaaaa ggcgatgtg 
      ttcagctac aaggaagac gacatggtg gaagacccg 
      aatctagtc cagcattta gcacactgg ggaataaat 
      atcgcgaat atggaaaaa accgaaaag tcgatggtc 
      gagctcgag ctggaattg aaccaaaaa tcgaacgaa 
      tggtcggct ttgcaggaa agcgacggc caattgaaa 
      cccatctac ggccctgga tacactgga atgaccaac 
      atgggcaac tcctgttac ctcaacagc gtgatgcaa 
      atggtattc tctattcca gatttcatc caaagatac 
      taccatgac gctgaatta tttttcaac aacgtggtt 
      ggtgaccca tgtgaagat tttaacgct caaatggct 
      aaattagct gttggtcta cattcggga aaatattca 
      gtcccacca cctgcagga tctcccgta gatgcggat 
      cctccagga atatgtccc ttgatgttc aaggctttg 
      gtgggtcga ggacatcca gaattctca acgaaaaag 
      caacaagac gcccaggag tttttcctc cacatactc 
      actctattg gagcggaac agcaaaaat aaagtcaat 
      ccgggcgat tgttttaaa tttaaagtg gaggagaaa 
      tttcaatgc agtgccagc aagaaagtc aagtatttg 
      acgaggtct gacgttctt ctgccactg atcatccca 
      atggatgca gccatcaat aaagacgag gttgcagcc 
      tacgaaacg aagaagcta gaggcggaa aaaacgggc 
      aaaaaactc ctcgctaac accatcgtg cggccaaaa 
      ataaatttc ctttcgtgt ttggaactc ttcacgcaa 
      tcggaagtc attaacaat ttttacagc tcggcagtc 
      aacgcaaac gttactgcc cggaaaact acgcgattg 
      gcaacattt ccagactac ctctgcatt cagttaaaa 
      aaattcatg ctgagggaa gactgggtt ccgattaaa 
      ctggacgtt tctattgaa atgccagac attttggac 
      atatcatcc ctgcgtggt actggcccc cagtcggac 
      gaagagctg cttccggag cccaaagtg cagcccccg 
      gcgcctgtg atggacgag ggggtcctg gcacagctg 
      gctgacatg ggttttccg cccgaggcc tgcaaaaga 
      gcggtgttt ttcacccat aactccggc ctggaggcg 
      gccaccgcc tggatcatg gagcacatc acggattcc 
      gatttttcg gacccgttt gttcctccc ggcacggag 
      agttcgcac tttacgccg aatgccgaa gctttggcg 
      agtattgtg gcgatgggg ttcacacaa catcaagca 
      actaaggct ctgaaggcg acggataat aatgtggaa 
      agagcgatg gattggatt ttttcgcat caggatgag 
      ttggataat gttacgtca ccgccgccg cccgaattc 
      agggacggg gacgggagg tataaattg gtggcgttt 
      gtttctcat atggggact tcaacaatg gtcggccac 
      tatgtggtc catattctc aaaaatggg caatgggtc 
      atctttaac gacaataag gtggctctg tcggaacgg 
      cccccgaag gatctaggt tatatgtat ttatatgag 
      cgactttaa
    mRNA sequence
    • >TC003401 RA
      tcatcgtca aaaaagccg tcaaacgaa ttgtcatca 
      ttgcaaaat tcaaattat tgtttaaaa gtattaaac 
      ccacaaacc atggaatta cttacaccg catttaagc 
      agcgttaga gtcccaaca gcgaccagc aaagtatac 
      aaggacgaa tgtgtcttc tctttcgat aatccggaa 
      accgagacc ggtctgtac gtaggtctg aagagtttt 
      ttcggactt gggcgcgat tatgttgaa aaacacttc 
      cacaaaacg ggagaagcc gtctatctc cacatccgg 
      cgagtgcgt catgaagtc tcatcaccc ccacagggc 
      gatggaccc gagaagaaa atcacgcgc ttggccatt 
      ggagttgac ggtggtttt gatccagag gccatgtca 
      aagaagttt gagtttgag gactcctac aatattgtc 
      gttttgcca ggtttcagc accatcccc tggcccaac 
      acagagctt ccccaaatt gtaaaatcg tctattgag 
      ggcattttg gaagccccc tcggccagt aaagtcgcc 
      gaaatggag gctttgacg gggacgtgg gatggcgaa 
      gctcgggtt gtctcccag tttgcccac aacttgcca 
      cagttagat aatgggaaa aaaattcca ccttctggg 
      tggcagtgc gaaaaatgt gataaaact gataatctg 
      tggctaaac ttaaccgat gggtctatc ttatgtggc 
      aggaggttt tatgatgga agtggagga aataatcac 
      gccattgaa cattataac gccaccggt catccctta 
      gctgttaaa ttggggacg atcacaaag gaaggaaaa 
      ggcgatgtg ttcagctac aaggaagac gacatggtg 
      gaagacccg aatctagtc cagcattta gcacactgg 
      ggaataaat atcgcgaat atggaaaaa accgaaaag 
      tcgatggtc gagctcgag ctggaattg aaccaaaaa 
      tcgaacgaa tggtcggct ttgcaggaa agcgacggc 
      caattgaaa cccatctac ggccctgga tacactgga 
      atgaccaac atgggcaac tcctgttac ctcaacagc 
      gtgatgcaa atggtattc tctattcca gatttcatc 
      caaagatac taccatgac gctgaatta tttttcaac 
      aacgtggtt ggtgaccca tgtgaagat tttaacgct 
      caaatggct aaattagct gttggtcta cattcggga 
      aaatattca gtcccacca cctgcagga tctcccgta 
      gatgcggat cctccagga atatgtccc ttgatgttc 
      aaggctttg gtgggtcga ggacatcca gaattctca 
      acgaaaaag caacaagac gcccaggag tttttcctc 
      cacatactc actctattg gagcggaac agcaaaaat 
      aaagtcaat ccgggcgat tgttttaaa tttaaagtg 
      gaggagaaa tttcaatgc agtgccagc aagaaagtc 
      aagtatttg acgaggtct gacgttctt ctgccactg 
      atcatccca atggatgca gccatcaat aaagacgag 
      gttgcagcc tacgaaacg aagaagcta gaggcggaa 
      aaaacgggc aaaaaactc ctcgctaac accatcgtg 
      cggccaaaa ataaatttc ctttcgtgt ttggaactc 
      ttcacgcaa tcggaagtc attaacaat ttttacagc 
      tcggcagtc aacgcaaac gttactgcc cggaaaact 
      acgcgattg gcaacattt ccagactac ctctgcatt 
      cagttaaaa aaattcatg ctgagggaa gactgggtt 
      ccgattaaa ctggacgtt tctattgaa atgccagac 
      attttggac atatcatcc ctgcgtggt actggcccc 
      cagtcggac gaagagctg cttccggag cccaaagtg 
      cagcccccg gcgcctgtg atggacgag ggggtcctg 
      gcacagctg gctgacatg ggttttccg cccgaggcc 
      tgcaaaaga gcggtgttt ttcacccat aactccggc 
      ctggaggcg gccaccgcc tggatcatg gagcacatc 
      acggattcc gatttttcg gacccgttt gttcctccc 
      ggcacggag agttcgcac tttacgccg aatgccgaa 
      gctttggcg agtattgtg gcgatgggg ttcacacaa 
      catcaagca actaaggct ctgaaggcg acggataat 
      aatgtggaa agagcgatg gattggatt ttttcgcat 
      caggatgag ttggataat gttacgtca ccgccgccg 
      cccgaattc agggacggg gacgggagg tataaattg 
      gtggcgttt gtttctcat atggggact tcaacaatg 
      gtcggccac tatgtggtc catattctc aaaaatggg 
      caatgggtc atctttaac gacaataag gtggctctg 
      tcggaacgg cccccgaag gatctaggt tatatgtat 
      ttatatgag cgactttaa gtagccaat gggtttgtt 
      tctctgatt ttgtatgta taaatacca aataaattg 
      tcaagattt tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY