Status
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Gene information
General information
TC003377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4140805..4143831
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003377
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03546

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdomen posterior # of remaining segments remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      remarks: later than schedule,
      Adult morphology
      remarks: later than schedule,
      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003377 RA
        MSPDSKKVC FKNFERQHK IPFVIYAHI ESARIPTPG 
        QKSVFVQKP CGFAYSIKC CYDRSLSKC ESYYGEDCV 
        SLLWKLLKR EAKEISEKL KTKVEPLGL NEEQINQIS 
        KLTKCYLCS EALHEDKDI DFHPLTGDY LGLGHKECR 
        GFRKIPKSI PVVVSKNGL TFLLGALSK GELDEILFI 
        CGNKEGRFG FMTPIPGFQ YLKFVESCK FLDEEFENQ 
        DRILSDSKK KGVRRWFKA LSEFDLMKR KTWVPFLYL 
        DSFDKLTRS DFPPPSGFY DELEDEEIS PEEYDRAVR 
        IWTVFGCET VGDYLKLCL ESSVLLLGD VFESFRMLC 
        LDKYKLDPF HYFTIASFA WDVMLKHIK VKLELLEDQ 
        EMIAFIKDN IRGGIVQCS KRYALANNR FCQLDQTKP 
        HTFIVYLDA NNLGGWAMS QCLPFSEFR WMSRNEIEE 
        IAPKVGILE EEGAYGYIF EVDLDYPKK LHEKHNDLP 
        FCYEHLTVP ELGQTVRIL NFYNKRNYV MDFRNLKQC 
        LSNGLILKK IHRVLQFKQ SKWLQPYIA YNTNLRASA 
        VSDIEKKLY KLLNNSVFG KTIQRLSTD KPIYAGFCI 
        LELSKKFMY DFHYKIMLS RYPQRINLL YMDTDSFIY 
        EIQTCNFFR DLQFDEKLL ELFDTSNLD KRWGIPPRN 
        KKVPGKMKD ECDGKVVRE FCGVGAKVY CLDVDGEEV 
        KKAMGVRKY VKRKYISVE DYRNCVLGD VTVRRGFLE 
        TGCKNYELK AYWVKRDVL MPGDKKRRI RENKVDTDA 
        WGPLVDD
      CDS sequence
      • >TC003377 RA
        atgtccccg gactctaaa aaagtgtgt tttaaaaat 
        ttcgaaaga caacacaag attcctttc gtaatttac 
        gcacatatt gaatccgca agaattcca acgcctggg 
        cagaagtca gtatttgtg caaaaacct tgcgggttt 
        gcttattca attaaatgt tgttacgac cgttccctg 
        tcaaaatgt gagagttac tacggagaa gactgcgtg 
        agtcttttg tggaagctt ttgaaaagg gaagcgaag 
        gaaataagt gagaaactg aaaacaaaa gtggaacct 
        ttgggcctg aacgaggaa caaatcaat caaatttcc 
        aaactaacc aaatgctac ctctgcagt gaggcttta 
        catgaagac aaagacatc gattttcat cccctaact 
        ggggactat ttgggatta gggcacaag gagtgtcga 
        gggtttcgc aaaatcccg aagtccatt ccagttgtg 
        gtctcaaaa aatggactg actttttta ctaggagcg 
        ctgagtaag ggcgaactt gacgaaata cttttcatt 
        tgtgggaac aaagaaggc agatttgga ttcatgact 
        ccaattcct ggcttccaa tatttgaaa tttgtggag 
        tcttgcaaa tttctcgac gaagagttt gaaaatcaa 
        gacaggatt ttgagcgat tccaaaaag aagggcgtc 
        agacggtgg tttaaagcc ctttccgag tttgatttg 
        atgaaaagg aagacttgg gtcccgttt ttgtatctg 
        gactctttc gacaaactc accagaagt gattttcct 
        ccaccttct ggcttctac gatgagctt gaagacgaa 
        gaaatttcg ccagaggag tatgaccgt gctgtgcgt 
        atttggacg gtttttggg tgtgaaact gtcggtgac 
        tatttaaaa ctttgcttg gaatccagc gtgttatta 
        ctgggagac gtcttcgaa agttttcga atgctgtgt 
        ttggataag tacaagtta gatccgttt cattacttc 
        acaattgcc tcgtttgcg tgggacgtt atgttgaaa 
        cacatcaaa gtcaaatta gagttgttg gaagatcag 
        gaaatgatt gcttttatc aaggataat atccgcggc 
        ggaattgta caatgttca aagcgatat gcacttgca 
        aataaccgg ttttgccaa cttgaccaa accaaaccg 
        cacacattt attgtgtat ttggacgcg aataatttg 
        ggagggtgg gcgatgtcg cagtgtttg cctttttcc 
        gaatttcgt tggatgagt cgaaacgaa attgaagaa 
        attgcgccc aaagtcgga atccttgaa gaagaggga 
        gcttacggt tacattttt gaagtcgac ttggattat 
        ccgaaaaag ttacacgag aaacacaac gatttgcct 
        ttttgttac gaacacttg actgtcccc gagctgggc 
        caaaccgtg cgcattttg aatttctac aataaacgg 
        aattacgtg atggatttc cggaattta aagcaatgt 
        ctttcgaac ggccttatc ctgaaaaaa atccaccgt 
        gtcttgcaa ttcaaacag tcaaagtgg ttgcaacct 
        tacatcgcc tacaacaca aacctgcgg gcaagtgct 
        gtgtctgac attgagaag aaactctac aaactgctc 
        aacaattct gttttcggg aaaaccatt cagagatta 
        agcaccgac aagccaatc tacgccggt ttctgcatc 
        ctagagtta tcgaaaaaa ttcatgtac gactttcac 
        tacaaaatc atgctttcc cgatacccc caaagaatt 
        aaccttctc tatatggac acagactcc tttatttac 
        gaaatccaa acatgcaat tttttccgc gacttgcag 
        tttgatgag aagttgttg gagcttttc gacacttcc 
        aatttggat aaacggtgg ggaatcccc ccaaggaac 
        aaaaaagtt ccgggaaaa atgaaagac gaatgtgat 
        gggaaagtc gtacgtgag ttttgcgga gtcggagct 
        aaggtttac tgtttggat gttgatggg gaagaggtg 
        aagaaggcc atgggggtg agaaaatat gtgaagagg 
        aagtatatt tcagtggag gactatcgg aattgtgtg 
        ttgggagat gttacagtt aggaggggt ttcctcgaa 
        acggggtgc aaaaattac gaactgaag gcgtattgg 
        gtgaaacga gatgtctta atgccggga gataaaaaa 
        cgccgtatt agggaaaat aaagttgat accgatgcg 
        tggggaccg ttggttgat gattaa
      mRNA sequence
      • >TC003377 RA
        ctataaaga agtgttaaa taaattaat gtggataaa 
        gaaacaact ggttgtttc tttgagttt tcggagaat 
        ttgtttatt ctaaaagca ctaaaagta accttcgct 
        gataataaa aacagctgc acaattgac atgccacat 
        taaagtgca atcaaggga agaaaaagc acaattcgt 
        ttgataaaa ccattaaag cccgctttc ctgtaccat 
        ttcttccaa cacatgtgc gggttaagt cgagacaaa 
        tggaatcac aagaccaag gtcgctggg agaaacagt 
        ctctaacag ttctaatct cttcctgta tgttttatt 
        gattactca aagcattca gtcgtaaat aaaattgtg 
        cttgcaaaa tgtccccgg actctaaaa aagtgtgtt 
        ttaaaaatt tcgaaagac aacacaaga ttcctttcg 
        taatttacg cacatattg aatccgcaa gaattccaa 
        cgcctgggc agaagtcag tatttgtgc aaaaacctt 
        gcgggtttg cttattcaa ttaaatgtt gttacgacc 
        gttccctgt caaaatgtg agagttact acggagaag 
        actgcgtga gtcttttgt ggaagcttt tgaaaaggg 
        aagcgaagg aaataagtg agaaactga aaacaaaag 
        tggaacctt tgggcctga acgaggaac aaatcaatc 
        aaatttcca aactaacca aatgctacc tctgcagtg 
        aggctttac atgaagaca aagacatcg attttcatc 
        ccctaactg gggactatt tgggattag ggcacaagg 
        agtgtcgag ggtttcgca aaatcccga agtccattc 
        cagttgtgg tctcaaaaa atggactga cttttttac 
        taggagcgc tgagtaagg gcgaacttg acgaaatac 
        ttttcattt gtgggaaca aagaaggca gatttggat 
        tcatgactc caattcctg gcttccaat atttgaaat 
        ttgtggagt cttgcaaat ttctcgacg aagagtttg 
        aaaatcaag acaggattt tgagcgatt ccaaaaaga 
        agggcgtca gacggtggt ttaaagccc tttccgagt 
        ttgatttga tgaaaagga agacttggg tcccgtttt 
        tgtatctgg actctttcg acaaactca ccagaagtg 
        attttcctc caccttctg gcttctacg atgagcttg 
        aagacgaag aaatttcgc cagaggagt atgaccgtg 
        ctgtgcgta tttggacgg tttttgggt gtgaaactg 
        tcggtgact atttaaaac tttgcttgg aatccagcg 
        tgttattac tgggagacg tcttcgaaa gttttcgaa 
        tgctgtgtt tggataagt acaagttag atccgtttc 
        attacttca caattgcct cgtttgcgt gggacgtta 
        tgttgaaac acatcaaag tcaaattag agttgttgg 
        aagatcagg aaatgattg cttttatca aggataata 
        tccgcggcg gaattgtac aatgttcaa agcgatatg 
        cacttgcaa ataaccggt tttgccaac ttgaccaaa 
        ccaaaccgc acacattta ttgtgtatt tggacgcga 
        ataatttgg gagggtggg cgatgtcgc agtgtttgc 
        ctttttccg aatttcgtt ggatgagtc gaaacgaaa 
        ttgaagaaa ttgcgccca aagtcggaa tccttgaag 
        aagagggag cttacggtt acatttttg aagtcgact 
        tggattatc cgaaaaagt tacacgaga aacacaacg 
        atttgcctt tttgttacg aacacttga ctgtccccg 
        agctgggcc aaaccgtgc gcattttga atttctaca 
        ataaacgga attacgtga tggatttcc ggaatttaa 
        agcaatgtc tttcgaacg gccttatcc tgaaaaaaa 
        tccaccgtg tcttgcaat tcaaacagt caaagtggt 
        tgcaacctt acatcgcct acaacacaa acctgcggg 
        caagtgctg tgtctgaca ttgagaaga aactctaca 
        aactgctca acaattctg ttttcggga aaaccattc 
        agagattaa gcaccgaca agccaatct acgccggtt 
        tctgcatcc tagagttat cgaaaaaat tcatgtacg 
        actttcact acaaaatca tgctttccc gataccccc 
        aaagaatta accttctct atatggaca cagactcct 
        ttatttacg aaatccaaa catgcaatt ttttccgcg 
        acttgcagt ttgatgaga agttgttgg agcttttcg 
        acacttcca atttggata aacggtggg gaatccccc 
        caaggaaca aaaaagttc cgggaaaaa tgaaagacg 
        aatgtgatg ggaaagtcg tacgtgagt tttgcggag 
        tcggagcta aggtttact gtttggatg ttgatgggg 
        aagaggtga agaaggcca tgggggtga gaaaatatg 
        tgaagagga agtatattt cagtggagg actatcgga 
        attgtgtgt tgggagatg ttacagtta ggaggggtt 
        tcctcgaaa cggggtgca aaaattacg aactgaagg 
        cgtattggg tgaaacgag atgtcttaa tgccgggag 
        ataaaaaac gccgtatta gggaaaata aagttgata 
        ccgatgcgt ggggaccgt tggttgatg attaaatga 
        cttaaataa tttattacg atatttttg gcccaaaat 
        aaatatttt tggatactt attttggaa cagaggtag 
        tattattta ttgttcttt gcttcaaca actttagtt 
        ttttagaaa tgtaatttt tctttacaa ggtgcaaac 
        agtcttaaa tgtataaca tatcagtat tttgctttg 
        tatgcgttt aactggtct caccagttc tattatttt 
        cttttaaaa cattttgga gtttatttt tgttattga 
        tttactatt tatcacaag tgactataa taaaatgtt 
        tagttttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY