Status
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Gene information
General information
TC032338
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003364,TC003365
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 1404966..1542187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032338
    iBeetle screen iB_03545
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    gut partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032338 RA
      MEGTFSASF GVLLYFMIF QGTGGVQRF EQQPSYTEV 
      NPGQDALLV CKVFNKRGS CSWQKDNKP MGMYPHKYE 
      WASGSQQNG DCSIWVRAA QLEFDDGNW ECQVTASDF 
      HTQDALTSQ PIHLVVRVP PQRPRIEYN GTQVLPGHN 
      LTALHGEVA VIKCVSHYG NPPPVLKWF LDQHEITPT 
      KQQTNTTEL DNPRTWLAL SVLELTISK ENHGKTLRC 
      VAVHESYST KSSSIEVRL DVMYVPETR LVGIPTTDI 
      EDLKDTVAI RCVVSANPK AAVVWRREG QSQPASLQE 
      LLQFSPAIR QHSGLYTCQ ARNKAGESQ PIRVQVDVK 
      YPPKIISIG RERVKTVTL FSPATFECL GEGNPQPTY 
      KWLQKLPTP FNRVEERGR DAKLYIANV TYDYQGEYR 
      CKVTNIIKG EERSELSEP IILQVHGAP QVLRQSATP 
      EVFVEKGEL ADLSMVVCA DPRPRVVAW EWGSLRLEA 
      GSEMGRYKV DEVVQEERE DCYLATLHI KDASATDSR 
      AYYLAVEND RGTDRHAVQ LYVNEPLQM STLVSLTGG 
      LLVTFLLLV CGCVYAVRT EKCCFARKG DFKPTDIDG 
      QKVDIEKTA TGPGGIPAD AIYTTTPRG HGSPEAMKQ 
      TTLTFNGRN SVEERPKEN VYSGLEPKT LHLRNRDLA 
      LNFFESDDN DTEKSFQDR SKYYSFNRY SGQKPFDNW 
      SYNTMAAGS HRASLPVDN KYVRRSQKK YNKKDRPRP 
      KPLQFENEK YYDHVFPKN RSSSTDLFY QPNSLDHAE 
      L
    CDS sequence
    • >TC032338 RA
      atggagggc acatttagc gctagcttt ggagtactc 
      ctttatttt atgatcttc caaggaacg ggcggcgta 
      caaaggttt gagcagcag ccctcatac accgaagtc 
      aatcccggc caggatgct cttcttgta tgcaaagtg 
      ttcaacaaa cgtggatcg tgttcttgg cagaaagat 
      aacaagccc atgggcatg tacccacac aaatacgaa 
      tgggcatcg ggaagtcag cagaacggg gattgttcc 
      atatgggtg cgtgctgcc cagttagag ttcgacgat 
      ggtaattgg gagtgtcag gtgacagcc agcgatttt 
      cacacacaa gacgcacta actagccaa ccaatccac 
      ctagtcgtt agagttccg ccacaacgg ccccgaata 
      gaatacaat ggcacgcaa gtgctcccc ggtcacaac 
      ttgaccgct ctccacggt gaagtggcc gtcattaag 
      tgcgtatcg cactacgga aacccacca cctgtactt 
      aagtggttt ttggatcag catgaaatt acgccgacg 
      aagcaacag accaacacc accgagttg gataatccg 
      aggacctgg ttggcgctg tcggtgctc gaactcacc 
      atctccaaa gaaaatcac ggaaaaacg ctcagatgt 
      gtcgcagta catgaatca tactcaacc aagtcgagc 
      agcatagaa gttagatta gacgtaatg tatgttcca 
      gagaccaga cttgtggga ataccaacc actgacatc 
      gaggacttg aaagacaca gttgccatc cgctgtgtc 
      gtcagcgcg aaccccaaa gctgccgtt gtgtggcgg 
      agagaaggt caaagtcaa cctgcgagc ttgcaggaa 
      ttattacaa tttagtcct gccattaga caacattca 
      ggcctgtat acgtgtcag gcccggaat aaagcggga 
      gaatcgcaa ccaattagg gtccaagtc gatgtcaaa 
      taccctcca aaaatcatc agcatcgga cgggaaaga 
      gtcaagact gtaacgtta ttttcgcca gccacattc 
      gagtgttta ggagaagga aatccacaa cccacatac 
      aagtggctg cagaaattg ccaacgcct ttcaatcga 
      gtcgaagaa cggggaagg gacgccaaa ttgtacatc 
      gccaatgtc acttacgac taccagggg gagtacagg 
      tgtaaagtt actaacatt atcaagggg gaggagagg 
      tcggaactc agcgagcca atcattttg caagtccat 
      ggtgcaccc caagtccta cggcaatca gcgacccca 
      gaagtgttc gtggaaaag ggcgagctt gcggatctt 
      agcatggta gtttgtgca gatcccagg cctcgagta 
      gttgcttgg gaatgggga tcgcttcgc ttggaagcc 
      gggtccgag atgggccga tacaaggtg gacgaggtg 
      gtgcaggaa gaacgagaa gactgttac ttggccact 
      ttgcacatc aaagatgct tcagcgact gattcaaga 
      gcgtattat ttagctgta gaaaatgat cgaggaact 
      gatcgacat gctgtacaa ctctacgtt aacgaaccc 
      ttgcaaatg agtacactc gtcagtctg acgggaggc 
      ctcctcgta accttcctt ctgctcgta tgtgggtgt 
      gtatatgcc gtccgaacc gaaaaatgc tgcttcgcc 
      cgtaagggc gacttcaaa ccgaccgat attgacggg 
      caaaaggtg gacatagag aaaaccgcc accggcccc 
      ggaggtatt cctgccgat gccatttat acgacaact 
      cctagaggt cacggcagc cccgaggcc atgaagcaa 
      accacactc acattcaat ggtagaaat agtgtagaa 
      gagagacca aaagaaaac gtctactct ggcctagaa 
      cccaaaact ctccacttg cgaaatcgc gatttagct 
      ctgaatttc ttcgagtca gacgataac gataccgaa 
      aagagtttc caggaccgg agcaagtac tatagtttt 
      aacagatac tcgggccaa aaacctttc gataactgg 
      tcttacaac acgatggcg gcgggctcg cacagggcc 
      tctctgccc gtagataac aaatacgtt cgccgatcg 
      cagaaaaaa tacaataaa aaagacagg cctagacct 
      aagcccttg cagttcgag aacgaaaag tattacgac 
      cacgtgttc cccaagaat aggtcgagc agtactgac 
      ttgttttat caaccaaat agcttagac catgcggag 
      ttgtaa
    mRNA sequence
    • >TC032338 RA
      taggttttt tttgtcact gttgccaac tttggtttt 
      gcatggggc agttaagta gcagcgcgt cgtcggcgt 
      cgaaggcac tgttgaagg agcgtcgga ggtgagcgt 
      cgaacttac gcgccttct ccaacttgc accaaactt 
      tgtcaaact gttccaagt gcgtctgta atctggatg 
      ggctcatcc tgacttgaa aaactacca ttaggcaaa 
      cttttatgt gtgtcgtgg gcaataacc gttcggtat 
      aaatagtgg taaattagt tttgtgaaa tgtaatttg 
      ctgacaata gccagtgac ttttaagaa atggagggc 
      acatttagc gctagcttt ggagtactc ctttatttt 
      atgatcttc caaggaacg ggcggcgta caaaggttt 
      gagcagcag ccctcatac accgaagtc aatcccggc 
      caggatgct cttcttgta tgcaaagtg ttcaacaaa 
      cgtggatcg tgttcttgg cagaaagat aacaagccc 
      atgggcatg tacccacac aaatacgaa tgggcatcg 
      ggaagtcag cagaacggg gattgttcc atatgggtg 
      cgtgctgcc cagttagag ttcgacgat ggtaattgg 
      gagtgtcag gtgacagcc agcgatttt cacacacaa 
      gacgcacta actagccaa ccaatccac ctagtcgtt 
      agagttccg ccacaacgg ccccgaata gaatacaat 
      ggcacgcaa gtgctcccc ggtcacaac ttgaccgct 
      ctccacggt gaagtggcc gtcattaag tgcgtatcg 
      cactacgga aacccacca cctgtactt aagtggttt 
      ttggatcag catgaaatt acgccgacg aagcaacag 
      accaacacc accgagttg gataatccg aggacctgg 
      ttggcgctg tcggtgctc gaactcacc atctccaaa 
      gaaaatcac ggaaaaacg ctcagatgt gtcgcagta 
      catgaatca tactcaacc aagtcgagc agcatagaa 
      gttagatta gacgtaatg tatgttcca gagaccaga 
      cttgtggga ataccaacc actgacatc gaggacttg 
      aaagacaca gttgccatc cgctgtgtc gtcagcgcg 
      aaccccaaa gctgccgtt gtgtggcgg agagaaggt 
      caaagtcaa cctgcgagc ttgcaggaa ttattacaa 
      tttagtcct gccattaga caacattca ggcctgtat 
      acgtgtcag gcccggaat aaagcggga gaatcgcaa 
      ccaattagg gtccaagtc gatgtcaaa taccctcca 
      aaaatcatc agcatcgga cgggaaaga gtcaagact 
      gtaacgtta ttttcgcca gccacattc gagtgttta 
      ggagaagga aatccacaa cccacatac aagtggctg 
      cagaaattg ccaacgcct ttcaatcga gtcgaagaa 
      cggggaagg gacgccaaa ttgtacatc gccaatgtc 
      acttacgac taccagggg gagtacagg tgtaaagtt 
      actaacatt atcaagggg gaggagagg tcggaactc 
      agcgagcca atcattttg caagtccat ggtgcaccc 
      caagtccta cggcaatca gcgacccca gaagtgttc 
      gtggaaaag ggcgagctt gcggatctt agcatggta 
      gtttgtgca gatcccagg cctcgagta gttgcttgg 
      gaatgggga tcgcttcgc ttggaagcc gggtccgag 
      atgggccga tacaaggtg gacgaggtg gtgcaggaa 
      gaacgagaa gactgttac ttggccact ttgcacatc 
      aaagatgct tcagcgact gattcaaga gcgtattat 
      ttagctgta gaaaatgat cgaggaact gatcgacat 
      gctgtacaa ctctacgtt aacgaaccc ttgcaaatg 
      agtacactc gtcagtctg acgggaggc ctcctcgta 
      accttcctt ctgctcgta tgtgggtgt gtatatgcc 
      gtccgaacc gaaaaatgc tgcttcgcc cgtaagggc 
      gacttcaaa ccgaccgat attgacggg caaaaggtg 
      gacatagag aaaaccgcc accggcccc ggaggtatt 
      cctgccgat gccatttat acgacaact cctagaggt 
      cacggcagc cccgaggcc atgaagcaa accacactc 
      acattcaat ggtagaaat agtgtagaa gagagacca 
      aaagaaaac gtctactct ggcctagaa cccaaaact 
      ctccacttg cgaaatcgc gatttagct ctgaatttc 
      ttcgagtca gacgataac gataccgaa aagagtttc 
      caggaccgg agcaagtac tatagtttt aacagatac 
      tcgggccaa aaacctttc gataactgg tcttacaac 
      acgatggcg gcgggctcg cacagggcc tctctgccc 
      gtagataac aaatacgtt cgccgatcg cagaaaaaa 
      tacaataaa aaagacagg cctagacct aagcccttg 
      cagttcgag aacgaaaag tattacgac cacgtgttc 
      cccaagaat aggtcgagc agtactgac ttgttttat 
      caaccaaat agcttagac catgcggag ttgtaaata 
      tacgtttaa gtcgtcgtc agattgttg tacatttta 
      atattaaat atgtaaata tctcgctgg gatttgttc 
      gttcgtttt catttctaa cattattta tttgataat 
      catactagt ccactgcac cttatgacg ttagaatta 
      aaccatttt tcacacact tctaccagt catggcgct 
      agcaagtag gtagaccgg gtcagaaac actggtttt 
      atgtcgttt caaatttta tgttgtgag tagctttaa 
      gacttttct acaggtgtg tgcctgcct tctacctac 
      tttgtgtat tagttttag gcgtttttt taacaaaaa 
      tttcccaaa cgtttgata gagctgtag ccctttttt 
      atattttta taatttagg tatttaatt acgtagacg 
      agttgattt tgtacgaac gtaaaccaa gtctaatgt 
      taatagata gagatcttc tgaatatat gtgatgtta 
      caataataa taaaatgac ttaaatcaa acgtgttat 
      tattcttag gctttaaat gtaaaatat aattaatta 
      ttatacaaa tcatataat ctgttatca tgtttaaaa 
      ttataaatt cgtgtcata tttttgatt ttttatgat 
      tgagaattt tatatcaag actaatatt gtttggtta 
      tttgttaga agtaaaacc atttgtatg atgacgtag 
      gagatctta ttttttaat gtaaaaaca acttatagc 
      attattata tgttataaa ataaattaa taataaaat 
      c