Status
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Gene information
General information
TC032336
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003361,TC003360
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2365559..2412292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032336
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032336 RA
      MDYVRNIFC FMTISLSLV YGQQQYFRV QPRDVKVQE 
      GGEAMLECE VANLAGQVQ WTKDGFALG FSSVIPGFP 
      RYSVIGDRR HGIYNLRVS NASLEDDAE YQCQVGPAR 
      LHKAIRANA RLNVISPPS SVEILDHRH NSKIEIKEN 
      QEFHLECRV RNAKPAAKI VWYRGNVEL NIPDREDQI 
      IEVPGKNGD KRVTRYDTH SRISLKPTA EDDYAEYTC 
      EARHEALSP DIPLRTTVQ LSVFYPPGP PYIEGYTEG 
      ETIRRGQTV ELVCRSRGG NPPAQLIWY KNGEQIRMA 
      YRTAGRVSE NVYTFTADA SDNKARYKC EASNIMSKA 
      PLKAEVDMT VLFSPAHVT ISGPTEARV GDPVPLTCT 
      TANSNPPAE IKWMVAGRQ VRNATSRTV VSPEGGWIT 
      TSNITAVVE PNRRSLVVI CHGLNMQLT ENVVSTHTI 
      NVLYPPSAP FIHGYTQGS YIPAGTVQK ISCTSSGGN 
      PLATLTWYK NDKKINSVL KTTDKSVTA EITILTNVT 
      DNEARYRCE AANSATEIP LFETITMNV YFPPDHVKI 
      RKEPLELKP NEQATLTCD SSSSNPPAK LSWWREGIP 
      VQGLLNTTK PGLHGGTVS SIELKLNIT EQLNGIVYT 
      CQATNEALQ RSVHDAITL QVLYKPVFD KDNEEAVTG 
      IEGEPLIIT LKADGNPQN IAYTWTKDG LPIIQSSSS 
      SGIERIISD GPVLNITRL SRHDAGTYS CEALNSQGS 
      SVAQVNITV QYAATIVAT SENVVVNPN EDATLSCTA 
      DGNPLSDDT ITWKRDDFP DFDARTSVM YDKNGTSYL 
      RITDVTRDD LGNFQCIVN NGVGNVTTK EVMLIVKHK 
      PEIDQHPAL LKFASDAGD TGRVVCRSQ ASPLAKYKW 
      ARSGAPITA NTTGKYYTT YRQIDALTS ESVLFITHV 
      TSSDYGNYE CVARNELGF ATASPRLEV TSAPDTPTL 
      LTILNVTHD TVTLGWTPG FDGGMKAAY RIRYRQVND 
      DGYKYVDVV PQNATSFLI RDLEVNTQY VFSIMAMNK 
      LGNSKYMPD LLSARTSMY SDHIEKIIP EDLLEKQDL 
      PRIVIISVS VIGIFLLIV NIGLVAGCM LKRRAKRIR 
      EQSNQTSKS ATIEMYAPS SYNDTVTGE TLSSVSEKS 
      ETYSNEGSN NDYVDEGRK QATSTYLMD QQDYPGAYP 
      VCPSYELQM QPNMAHKTH TLPHPHHHH QHHMHDRNR 
      ARDDQSLAY HGTMSAKSS YVSAPSPAP PADGSYYNM 
      SDRYLSYPP PLDFQNPPP IPAHPHMGH MHNPVTPPL 
      PANGSLLRH QRGAVPPDV LHNTSQNPQ ILQAQTLAQ 
      KRELTSFGN GYGVNEQEG HLV
    CDS sequence
    • >TC032336 RA
      atggattat gtgcgaaac atattctgc ttcatgacc 
      attagtctg agtttggtt tatggccag caacagtac 
      ttcagagtt cagccccgg gacgtcaaa gtgcaggag 
      ggtggcgaa gcgatgctg gaatgcgaa gttgccaat 
      ctggcaggt caagtgcaa tggaccaag gacggattt 
      gccttaggg ttctcgtcg gtcatcccg ggtttccct 
      cgatactct gtgatcggt gaccgtcgt catggcata 
      tataacttg agggtgagc aatgcttcc cttgaggac 
      gacgccgaa taccagtgt caagtggga ccggcaaga 
      cttcataag gcaattcga gcaaacgcc agactgaac 
      gtcatatcg ccgccgtcg tccgtcgaa attttggac 
      catcggcat aattccaag attgaaata aaggaaaat 
      caagagttt catcttgaa tgcagggtt cggaacgcc 
      aaacccgcc gccaaaatc gtttggtac agaggaaac 
      gtggaacta aatataccc gatcgcgag gaccaaata 
      attgaggtc cccggcaaa aacggcgac aaaagggtc 
      accagatac gacacgcac tcccgtatc tctttgaag 
      ccaacagct gaggatgat tacgcggaa tacacgtgc 
      gaagcgcgc cacgaagcg ttatccccc gacatcccg 
      ttacgaaca accgtacag ttgagcgtt ttctatccg 
      ccgggcccg ccctacatc gagggctac acggaaggc 
      gagaccatc cgccgcggc cagaccgtg gagctggtg 
      tgccgcagt cggggcggc aatcccccg gcccagctc 
      atctggtac aaaaacggg gagcagatc cgcatggcc 
      tacaggacg gcgggccgc gtgtccgag aacgtctac 
      acgttcacg gccgacgcc agcgacaac aaggcgcgc 
      tacaagtgc gaggcgagc aacatcatg tccaaggcg 
      cccctcaaa gccgaagtg gatatgacg gtgctattt 
      tcgccggca catgtcacc atatcaggg ccgaccgaa 
      gccagggtt ggcgatccg gtgcctctc acttgcacg 
      accgcaaac tccaatccg cccgctgaa attaaatgg 
      atggtggcc ggcaggcag gttagaaat gccacctcc 
      aggacggtc gtctccccg gaaggaggc tggattaca 
      acttcgaac atcacagcc gttgttgaa ccgaatcgg 
      agaagtttg gtcgtgatt tgtcacgga ctgaacatg 
      caattaacc gaaaatgtt gtctcgacg catactatc 
      aacgtttta tatccacca agtgcccct tttatccac 
      ggctacacg cagggctcg tacatccct gccggaacc 
      gttcaaaaa atctcctgc acctcatcc gggggcaac 
      cccttagcc accctaacg tggtacaag aacgacaaa 
      aagataaat tcagtgttg aaaacgacg gacaagtca 
      gtaactgca gaaataaca attctgaca aatgtaacc 
      gataatgag gcgcgatat cggtgcgaa gcagcaaac 
      tcagctact gaaatcccg ctcttcgaa acgataaca 
      atgaatgtt tactttcca cctgatcac gtgaaaatt 
      cgtaaagaa ccgcttgaa ctgaaaccg aacgagcaa 
      gcgactcta acttgcgac tcgagctcg agcaatcct 
      ccagcgaaa ttatcgtgg tggcgtgag ggcatccca 
      gtccaaggt ctcctcaat acgaccaaa cccgggctt 
      catgggggc acagtttcc agtattgag ctcaaactc 
      aacatcacg gaacaactc aatgggatt gtttacacg 
      tgtcaagcc acgaatgag gcgctgcaa cgctcggtc 
      catgacgcc atcaccctg caagtttta tataagccc 
      gtcttcgac aaagataac gaagaagcg gtcacgggc 
      atagaaggc gagccatta ataatcacg ttaaaagca 
      gacggcaat ccgcagaac atcgcctac acctggaca 
      aaggacggc ctcccgatt atccaatcg tcctcgagt 
      tcgggcatc gagaggatt atctccgac ggtccggtt 
      ttaaatata accagatta agccgccac gacgctgga 
      acctactct tgcgaagcc ctgaactcg cagggcagt 
      tcagtggcc caagttaac atcaccgtc caatacgca 
      gcgacaatc gtcgccacg agcgaaaat gtcgtcgtg 
      aatcccaat gaggacgcg acgctttcc tgcacagcg 
      gacgggaat ccgctgtct gatgacaca attacgtgg 
      aaaagggac gattttccg gattttgac gcgcgcacc 
      tccgttatg tacgataaa aacgggacg tcgtatctg 
      cggataact gatgttaca agggacgat ttggggaac 
      tttcaatgc atcgtcaac aacggcgtg ggaaatgtc 
      accacgaaa gaagtgatg ctcattgtc aagcataaa 
      cccgaaatt gatcagcat ccggctctt cttaaattt 
      gcaagtgat gcgggcgac accgggaga gtggtttgc 
      cggagccag gcctcgcct ttggccaag tacaaatgg 
      gctaggtcg ggggcgccc atcacggct aataccacc 
      gggaaatac tacaccacc tacagacag atcgacgct 
      cttacatcg gaatccgtc ctgttcatc acccatgtg 
      acctcctcc gactacggc aactacgaa tgcgttgcc 
      cgcaacgaa ttgggattt gcgaccgct tctcctcgc 
      cttgaagta acttctgct ccagatact cccacactg 
      ctgacaatc ctaaacgtg actcatgat accgttacg 
      ttaggatgg acacctggt tttgatgga ggcatgaaa 
      gccgcttat cgcattaga tacagacaa gtaaacgat 
      gatggctat aagtacgtg gacgtcgtg ccacagaac 
      gccacttct ttcctgata cgagatttg gaagttaac 
      acacaatac gtgttctcc attatggcg atgaataaa 
      ttaggcaac agcaagtac atgcccgat cttttatca 
      gcccggacg tcaatgtac agcgaccac attgagaag 
      attatcccc gaggatttg ctcgagaag caagacttg 
      cccagaatt gttattatt agtgtttct gttattggt 
      attttcctt ttgattgtc aacatcgga cttgttgcc 
      ggttgcatg ctaaagcga cgagctaag cgaatacga 
      gaacaaagt aaccaaacg agcaaatca gcaaccatc 
      gaaatgtac gcccccagc agctacaac gacacggtc 
      actggagag accttatct tcggttagc gagaaaagt 
      gagacttat agcaacgaa ggaagcaac aacgattac 
      gtcgatgaa gggcgcaag caagcaacg agcacttat 
      ctaatggac cagcaggac tacccaggt gcgtaccca 
      gtgtgtccc agttacgag cttcagatg caacctaac 
      atggctcac aaaactcac actcttcct catcctcat 
      catcaccac cagcatcac atgcatgat aggaacaga 
      gctagagat gaccagagt cttgcctat catggcacg 
      atgtcagcg aaatcgtcg tacgtgagc gcgccctcg 
      ccagcgccc ccagccgac ggttcatac tacaacatg 
      tccgaccgc tacttgtca tatccgccg ccgctggac 
      ttccagaat ccgcccccg attccggcg cacccccac 
      atggggcac atgcacaac ccggtgacg ccgcccctg 
      cccgccaac ggctccctg ctgcgccac cagcgaggg 
      gccgttccg cccgacgtc ctgcacaac accagccaa 
      aacccccaa atactccaa gctcaaact ctcgctcaa 
      aagcgggag ctcaccagt tttggcaac ggttacggt 
      gttaacgaa caggaagga catcttgtc tga
    mRNA sequence
    • >TC032336 RA
      ggtggagtc agggcagct ccgggcgaa ctggacaca 
      aatccggtt taagttaac tcgatcaaa acaaatcgc 
      gaggaaatt cctgcaact gcaggatta gtcgagagt 
      gtgccgcga aatcgggcg gagtcattc ataaattca 
      cctggtgtg aagtaacca ttcatggat tcggtgctt 
      taacggtgt gtgaagcca gcgttattt gtaataaca 
      attttaact atgtggttc attagtgtt actaccggt 
      ttaactctt cagccaacc tgggttgct ttaagtttt 
      cagtgaggt gacttcaat gatgcgtga gcgtgaaac 
      ggccacaaa aagttctgt gataatttc ggtggatca 
      gtgatattt gtgatagat ttttccggg gatacacta 
      actcaaata tggattatg tgcgaaaca tattctgct 
      tcatgacca ttagtctga gtttggttt atggccagc 
      aacagtact tcagagttc agccccggg acgtcaaag 
      tgcaggagg gtggcgaag cgatgctgg aatgcgaag 
      ttgccaatc tggcaggtc aagtgcaat ggaccaagg 
      acggatttg ccttagggt tctcgtcgg tcatcccgg 
      gtttccctc gatactctg tgatcggtg accgtcgtc 
      atggcatat ataacttga gggtgagca atgcttccc 
      ttgaggacg acgccgaat accagtgtc aagtgggac 
      cggcaagac ttcataagg caattcgag caaacgcca 
      gactgaacg tcatatcgc cgccgtcgt ccgtcgaaa 
      ttttggacc atcggcata attccaaga ttgaaataa 
      aggaaaatc aagagtttc atcttgaat gcagggttc 
      ggaacgcca aacccgccg ccaaaatcg tttggtaca 
      gaggaaacg tggaactaa atatacccg atcgcgagg 
      accaaataa ttgaggtcc ccggcaaaa acggcgaca 
      aaagggtca ccagatacg acacgcact cccgtatct 
      ctttgaagc caacagctg aggatgatt acgcggaat 
      acacgtgcg aagcgcgcc acgaagcgt tatcccccg 
      acatcccgt tacgaacaa ccgtacagt tgagcgttt 
      tctatccgc cgggcccgc cctacatcg agggctaca 
      cggaaggcg agaccatcc gccgcggcc agaccgtgg 
      agctggtgt gccgcagtc ggggcggca atcccccgg 
      cccagctca tctggtaca aaaacgggg agcagatcc 
      gcatggcct acaggacgg cgggccgcg tgtccgaga 
      acgtctaca cgttcacgg ccgacgcca gcgacaaca 
      aggcgcgct acaagtgcg aggcgagca acatcatgt 
      ccaaggcgc ccctcaaag ccgaagtgg atatgacgg 
      tgctatttt cgccggcac atgtcacca tatcagggc 
      cgaccgaag ccagggttg gcgatccgg tgcctctca 
      cttgcacga ccgcaaact ccaatccgc ccgctgaaa 
      ttaaatgga tggtggccg gcaggcagg ttagaaatg 
      ccacctcca ggacggtcg tctccccgg aaggaggct 
      ggattacaa cttcgaaca tcacagccg ttgttgaac 
      cgaatcgga gaagtttgg tcgtgattt gtcacggac 
      tgaacatgc aattaaccg aaaatgttg tctcgacgc 
      atactatca acgttttat atccaccaa gtgcccctt 
      ttatccacg gctacacgc agggctcgt acatccctg 
      ccggaaccg ttcaaaaaa tctcctgca cctcatccg 
      ggggcaacc ccttagcca ccctaacgt ggtacaaga 
      acgacaaaa agataaatt cagtgttga aaacgacgg 
      acaagtcag taactgcag aaataacaa ttctgacaa 
      atgtaaccg ataatgagg cgcgatatc ggtgcgaag 
      cagcaaact cagctactg aaatcccgc tcttcgaaa 
      cgataacaa tgaatgttt actttccac ctgatcacg 
      tgaaaattc gtaaagaac cgcttgaac tgaaaccga 
      acgagcaag cgactctaa cttgcgact cgagctcga 
      gcaatcctc cagcgaaat tatcgtggt ggcgtgagg 
      gcatcccag tccaaggtc tcctcaata cgaccaaac 
      ccgggcttc atgggggca cagtttcca gtattgagc 
      tcaaactca acatcacgg aacaactca atgggattg 
      tttacacgt gtcaagcca cgaatgagg cgctgcaac 
      gctcggtcc atgacgcca tcaccctgc aagttttat 
      ataagcccg tcttcgaca aagataacg aagaagcgg 
      tcacgggca tagaaggcg agccattaa taatcacgt 
      taaaagcag acggcaatc cgcagaaca tcgcctaca 
      cctggacaa aggacggcc tcccgatta tccaatcgt 
      cctcgagtt cgggcatcg agaggatta tctccgacg 
      gtccggttt taaatataa ccagattaa gccgccacg 
      acgctggaa cctactctt gcgaagccc tgaactcgc 
      agggcagtt cagtggccc aagttaaca tcaccgtcc 
      aatacgcag cgacaatcg tcgccacga gcgaaaatg 
      tcgtcgtga atcccaatg aggacgcga cgctttcct 
      gcacagcgg acgggaatc cgctgtctg atgacacaa 
      ttacgtgga aaagggacg attttccgg attttgacg 
      cgcgcacct ccgttatgt acgataaaa acgggacgt 
      cgtatctgc ggataactg atgttacaa gggacgatt 
      tggggaact ttcaatgca tcgtcaaca acggcgtgg 
      gaaatgtca ccacgaaag aagtgatgc tcattgtca 
      agcataaac ccgaaattg atcagcatc cggctcttc 
      ttaaatttg caagtgatg cgggcgaca ccgggagag 
      tggtttgcc ggagccagg cctcgcctt tggccaagt 
      acaaatggg ctaggtcgg gggcgccca tcacggcta 
      ataccaccg ggaaatact acaccacct acagacaga 
      tcgacgctc ttacatcgg aatccgtcc tgttcatca 
      cccatgtga cctcctccg actacggca actacgaat 
      gcgttgccc gcaacgaat tgggatttg cgaccgctt 
      ctcctcgcc ttgaagtaa cttctgctc cagatactc 
      ccacactgc tgacaatcc taaacgtga ctcatgata 
      ccgttacgt taggatgga cacctggtt ttgatggag 
      gcatgaaag ccgcttatc gcattagat acagacaag 
      taaacgatg atggctata agtacgtgg acgtcgtgc 
      cacagaacg ccacttctt tcctgatac gagatttgg 
      aagttaaca cacaatacg tgttctcca ttatggcga 
      tgaataaat taggcaaca gcaagtaca tgcccgatc 
      ttttatcag cccggacgt caatgtaca gcgaccaca 
      ttgagaaga ttatccccg aggatttgc tcgagaagc 
      aagacttgc ccagaattg ttattatta gtgtttctg 
      ttattggta ttttccttt tgattgtca acatcggac 
      ttgttgccg gttgcatgc taaagcgac gagctaagc 
      gaatacgag aacaaagta accaaacga gcaaatcag 
      caaccatcg aaatgtacg cccccagca gctacaacg 
      acacggtca ctggagaga ccttatctt cggttagcg 
      agaaaagtg agacttata gcaacgaag gaagcaaca 
      acgattacg tcgatgaag ggcgcaagc aagcaacga 
      gcacttatc taatggacc agcaggact acccaggtg 
      cgtacccag tgtgtccca gttacgagc ttcagatgc 
      aacctaaca tggctcaca aaactcaca ctcttcctc 
      atcctcatc atcaccacc agcatcaca tgcatgata 
      ggaacagag ctagagatg accagagtc ttgcctatc 
      atggcacga tgtcagcga aatcgtcgt acgtgagcg 
      cgccctcgc cagcgcccc cagccgacg gttcatact 
      acaacatgt ccgaccgct acttgtcat atccgccgc 
      cgctggact tccagaatc cgcccccga ttccggcgc 
      acccccaca tggggcaca tgcacaacc cggtgacgc 
      cgcccctgc ccgccaacg gctccctgc tgcgccacc 
      agcgagggg ccgttccgc ccgacgtcc tgcacaaca 
      ccagccaaa acccccaaa tactccaag ctcaaactc 
      tcgctcaaa agcgggagc tcaccagtt ttggcaacg 
      gttacggtg ttaacgaac aggaaggac atcttgtct 
      gagacaagg aggacattt tgtatgtcc tgtaaaata 
      aagtgcgac tgattcgga gttaaacag actgtgagt 
      tctaatgaa tgataatat cactgcaat ataaagaca 
      cggataatc aattaacag tgtattttg ctaatttta 
      cttaaagaa actataaat aattaacag tgttgttct 
      agagttgtt acgtgttga attattgtc agtactgaa 
      gaaaagcgc ataaggaga taataatgc aactgtagg 
      ttaagtact tagatttgt ttgcagatt tactaaaat 
      actcgtgta aaattgtga taactctca tttcgtctg 
      ttattatct tcttatgct ttgcaataa tttaactga 
      atgatttcg tcacgaaat aaaacgaat aacttgcta 
      actgataat cagtttatt taaaattaa tgtgatcgt 
      tgtatgtaa agaattatt gttattttt gtaagaagc 
      ttcttttgc aacattatt atcacttaa cgttcttat 
      tctaagttg tagcgttta gttttttgt acatacttt 
      ccgttaagg tttgaataa tttaaaaaa tgttattta 
      ctagtccaa tccaacaac caaagtgtt tcttttgta 
      catgaacgc cttttaatt tgagtgtaa ctactttaa 
      atttcattt gtagctttc tattttctc tttatttat 
      tcttttatc tttatttgt acacaactt tgttacgtt 
      aataaattt tgtacctat atcaagaat acttttgtg 
      ttgtttttt tttattttc cctttcgtt ttgagtaac 
      gaggcaata ttacggctt aaatcaaaa tagacgcag 
      taaaaattg atctctacc acgaatgta ggaaacaac 
      gaaacggcc aattttgat caagcattt atgtttagt 
      ttgtactag aaacggaca aatggggta tgaatacaa 
      attctctga aacataaaa ttcgaagcg ttatcattt 
      gctgaataa aattgttat agtcgagaa acaatcatt 
      tttatagac tattatgaa gttttatag gtggactgt 
      aaccttcgt ttaaaccaa ttaatcaaa ggtgtaaat 
      atgtaaaat gtattataa aatatgaaa ctaatacct 
      tccattttg ccattgtaa taagaataa taataaatg 
      tttaaaagt gcctttact attaaaaca atctgctgt 
      gtattatat tagtgcagt gttttaatt ttaggttta 
      aatcacgat tgtcgcatt tcggttcgt tataggtaa 
      ctgtaaata attttcgtc aattctcta attacctac 
      cgtaacttt acaaaaata tttttctca gtgtgcttg 
      taattcgga gaattggtt ttacagttg tacctatat 
      tttcccttt attgttgtt gatacgaat gaatgctca 
      ttgtaaatt atactgttg aattgtcca gtcaatata 
      aacttgtat gtctg
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