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Gene information
General information
TC003355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 1005070..1022227
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003355
    iBeetle screen iB_03543
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003355 RA
      MWSNSLTAG CNPDSELFP GFGSTCGGS SSSMAYLKS 
      APYPVPGLG LHGLPVDSL HSSMAGYPA GNQRKQRRE 
      RTTFTRAQL DVLEALFGK TRYPDIFMR EEVALKINL 
      PESRVQVWF KNRRAKCRQ QQKQHNQQQ SVEKSSKLK 
      NKSAPILTK TSPTPVSIN NNTSTSSSA SSPSVTAAP 
      HLRDSPNYI KPQLHVSTG STSSPTIAS ATYTNSANS 
      SIWSPASID SFTLEQHRS WCSSSQPVL STTNSTTNC 
      YNNYPYYSN MDYLSSSTM SHSQFGENG LETSWGKSR 
      DESSWFYNS GWERK
    • >TC003355 RB
      MWSNSLTAG CNPDSELFP GFGSTCGGS SSSMAYLKS 
      APYPVPGLG LHGLPVDSL HSSMAGYPA GNQRKQRRE 
      RTTFTRAQL DVLEALFGK TRYPDIFMR EEVALKINL 
      PESRVQITF GNRHPKATS LSVWFKNRR AKCRQQQKQ 
      HNQQQSVEK SSKLKNKSA PILTKTSPT PVSINNNTS 
      TSSSASSPS VTAAPHLRD SPNYIKPQL HVSTGSTSS 
      PTIASATYT NSANSSIWS PASIDSFTL EQHRSWCSS 
      SQPVLSTTN STTNCYNNY PYYSNMDYL SSSTMSHSQ 
      FGENGLETS WGKSRDESS WFYNSGWER K
    CDS sequence
    • >TC003355 RA
      atgtggtca aacagcctc actgctgga tgcaacccc 
      gacagcgaa ttatttccg ggttttgga agcacttgc 
      ggcggaagt tcctcaagt atggcctat ttaaagtcg 
      gccccttat cccgtgcca ggcttaggt ttacatgga 
      ctcccagta gactccctc cactcgtcc atggctgga 
      tatccagca ggtaatcaa cgaaaacag cgtcgggag 
      agaacgacc ttcaccagg gctcaactt gacgtcctg 
      gaggccctc ttcggaaag acgcgatat cctgacatt 
      tttatgaga gaagaggtc gcactcaaa attaatcta 
      ccggaatcc agagttcag gtgtggttc aaaaatcgc 
      cgggcaaag tgccgccag cagcaaaag cagcacaac 
      cagcaacaa agcgtggaa aaaagctcg aaacttaaa 
      aacaaaagt gctcccatt ttaacaaaa acatcacca 
      acaccagtt tcgataaac aacaacacg agtacgagt 
      tcttcagcg agctcaccg agcgtcact gcagcgccc 
      caccttaga gacagcccc aactacatc aagccgcaa 
      ctacacgta tctacaggg agtacgagc tcaccaacg 
      attgcgtca gcaacttac acaaattcg gcaaattcg 
      agcatttgg tcgccagcc tcgatagat agctttact 
      ttggagcaa cacaggtcc tggtgttcg tcctcacag 
      ccggtgcta agcaccact aactcgacc acaaactgt 
      tataacaat tacccttat tactccaat atggattat 
      ctgagttcg tcgacaatg agccactcg cagttcggg 
      gagaacggc ttagaaacc agttggggg aaatcccgg 
      gacgagtcc tcctggttc tacaactcc ggatgggag 
      cggaaatga
    • >TC003355 RB
      atgtggtca aacagcctc actgctgga tgcaacccc 
      gacagcgaa ttatttccg ggttttgga agcacttgc 
      ggcggaagt tcctcaagt atggcctat ttaaagtcg 
      gccccttat cccgtgcca ggcttaggt ttacatgga 
      ctcccagta gactccctc cactcgtcc atggctgga 
      tatccagca ggtaatcaa cgaaaacag cgtcgggag 
      agaacgacc ttcaccagg gctcaactt gacgtcctg 
      gaggccctc ttcggaaag acgcgatat cctgacatt 
      tttatgaga gaagaggtc gcactcaaa attaatcta 
      ccggaatcc agagttcag atcactttc ggtaaccgg 
      cacccaaaa gcgacttca ctttcggtg tggttcaaa 
      aatcgccgg gcaaagtgc cgccagcag caaaagcag 
      cacaaccag caacaaagc gtggaaaaa agctcgaaa 
      cttaaaaac aaaagtgct cccatttta acaaaaaca 
      tcaccaaca ccagtttcg ataaacaac aacacgagt 
      acgagttct tcagcgagc tcaccgagc gtcactgca 
      gcgccccac cttagagac agccccaac tacatcaag 
      ccgcaacta cacgtatct acagggagt acgagctca 
      ccaacgatt gcgtcagca acttacaca aattcggca 
      aattcgagc atttggtcg ccagcctcg atagatagc 
      tttactttg gagcaacac aggtcctgg tgttcgtcc 
      tcacagccg gtgctaagc accactaac tcgaccaca 
      aactgttat aacaattac ccttattac tccaatatg 
      gattatctg agttcgtcg acaatgagc cactcgcag 
      ttcggggag aacggctta gaaaccagt tgggggaaa 
      tcccgggac gagtcctcc tggttctac aactccgga 
      tgggagcgg aaatga
    mRNA sequence
    • >TC003355 RA
      ctcatctgt ttctttgca cctcttttt ttcccaaag 
      ggaccaaag tagatgtgg tcaaacagc ctcactgct 
      ggatgcaac cccgacagc gaattattt ccgggtttt 
      ggaagcact tgcggcgga agttcctca agtatggcc 
      tatttaaag tcggcccct tatcccgtg ccaggctta 
      ggtttacat ggactccca gtagactcc ctccactcg 
      tccatggct ggatatcca gcaggtaat caacgaaaa 
      cagcgtcgg gagagaacg accttcacc agggctcaa 
      cttgacgtc ctggaggcc ctcttcgga aagacgcga 
      tatcctgac atttttatg agagaagag gtcgcactc 
      aaaattaat ctaccggaa tccagagtt caggtgtgg 
      ttcaaaaat cgccgggca aagtgccgc cagcagcaa 
      aagcagcac aaccagcaa caaagcgtg gaaaaaagc 
      tcgaaactt aaaaacaaa agtgctccc attttaaca 
      aaaacatca ccaacacca gtttcgata aacaacaac 
      acgagtacg agttcttca gcgagctca ccgagcgtc 
      actgcagcg ccccacctt agagacagc cccaactac 
      atcaagccg caactacac gtatctaca gggagtacg 
      agctcacca acgattgcg tcagcaact tacacaaat 
      tcggcaaat tcgagcatt tggtcgcca gcctcgata 
      gatagcttt actttggag caacacagg tcctggtgt 
      tcgtcctca cagccggtg ctaagcacc actaactcg 
      accacaaac tgttataac aattaccct tattactcc 
      aatatggat tatctgagt tcgtcgaca atgagccac 
      tcgcagttc ggggagaac ggcttagaa accagttgg 
      gggaaatcc cgggacgag tcctcctgg ttctacaac 
      tccggatgg gagcggaaa tgaaaagaa acagccggg 
      ggaagcaaa gagaattgc aatttttag tcaaatctt 
      tcatgtaac taactaact gcttcgatt catcactat 
      atcaatcaa aattgcaat aggagatat ttttgtaag 
      tttggttca gccaacgtg caataactt ggggtttca 
      tatcattag ttgtgtata tacctacta gatggaaca 
      ttctgtaca gtgttagta tagttgtac gagtagttg 
      tatagtttt gaccaatac aataaattt tacgaattt 
      at
    • >TC003355 RB
      ctcatctgt ttctttgca cctcttttt ttcccaaag 
      ggaccaaag tagatgtgg tcaaacagc ctcactgct 
      ggatgcaac cccgacagc gaattattt ccgggtttt 
      ggaagcact tgcggcgga agttcctca agtatggcc 
      tatttaaag tcggcccct tatcccgtg ccaggctta 
      ggtttacat ggactccca gtagactcc ctccactcg 
      tccatggct ggatatcca gcaggtaat caacgaaaa 
      cagcgtcgg gagagaacg accttcacc agggctcaa 
      cttgacgtc ctggaggcc ctcttcgga aagacgcga 
      tatcctgac atttttatg agagaagag gtcgcactc 
      aaaattaat ctaccggaa tccagagtt cagatcact 
      ttcggtaac cggcaccca aaagcgact tcactttcg 
      gtgtggttc aaaaatcgc cgggcaaag tgccgccag 
      cagcaaaag cagcacaac cagcaacaa agcgtggaa 
      aaaagctcg aaacttaaa aacaaaagt gctcccatt 
      ttaacaaaa acatcacca acaccagtt tcgataaac 
      aacaacacg agtacgagt tcttcagcg agctcaccg 
      agcgtcact gcagcgccc caccttaga gacagcccc 
      aactacatc aagccgcaa ctacacgta tctacaggg 
      agtacgagc tcaccaacg attgcgtca gcaacttac 
      acaaattcg gcaaattcg agcatttgg tcgccagcc 
      tcgatagat agctttact ttggagcaa cacaggtcc 
      tggtgttcg tcctcacag ccggtgcta agcaccact 
      aactcgacc acaaactgt tataacaat tacccttat 
      tactccaat atggattat ctgagttcg tcgacaatg 
      agccactcg cagttcggg gagaacggc ttagaaacc 
      agttggggg aaatcccgg gacgagtcc tcctggttc 
      tacaactcc ggatgggag cggaaatga aaagaaaca 
      gccggggga agcaaagag aattgcaat ttttagtca 
      aatctttca tgtaactaa ctaactgct tcgattcat 
      cactatatc aatcaaaat tgcaatagg agatatttt 
      tgtaagttt ggttcagcc aacgtgcaa taacttggg 
      gtttcatat cattagttg tgtatatac ctactagat 
      ggaacattc tgtacagtg ttagtatag ttgtacgag 
      tagttgtat agttttgac caatacaat aaattttac 
      gaatttat