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Gene information
General information
TC003331 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 276376..279740
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003331
    iBeetle screen iB_03540
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    remarks: later than schedule,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost, remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003331 RA
      MGETSTENS TTVSDYVGG GLWITVYCI IIVCAIAGN 
TLTLFAIAA SRQLSSMVS NQFIFSLGV SDLLVGLSI 
PYHMCFYLT DSIIHNETT CLARFVLIS FACASSILN 
LLFVATDRY TAIVHPLKY NQYMTRQNA TFLISLVWI 
LSLTFSTVP IYWNTWTEE KTCDVQIIP EVYFNFVLT 
PMFVLIWGV MLLVYIKIW REASKHAKR IRSTTNLHK 
CHNINDTKS IQVVMLTLG CFSICWMPY FIVTTYFRI 
SGSSSQFSL LYEIAFTLA VCNSGMNPV IYAWKNSNF 
REAFWCLLT CHSPNNSTK KTNYITNHV PSSKKSSIG 
GHDNVVAQT EIEIESPTP KDYDEHECS VSTEMTTVN 
TSTWS
    CDS sequence
    • >TC003331 RA
      atgggagaa acgtcgacc gaaaattcc accacagtg 
tccgattat gtaggtggt ggtttgtgg ataacagtg 
tactgtata attatcgtt tgtgctatt gctggcaac 
actttgaca cttttcgca atagcggcc agccgacag 
ttgtcgtcg atggtgtcc aaccaattc atcttcagc 
ttgggagtg agcgactta ttggtgggg ctgtccatc 
ccttaccat atgtgcttc tacttgacg gactcaatc 
attcacaac gaaaccact tgtttggcc cgatttgtc 
ctcatatct ttcgcttgt gcaagctcg atattaaac 
cttcttttc gtcgccacg gacagatat acggccatc 
gtccatccc ttgaaatac aaccagtat atgacgcgg 
caaaatgca acttttctc atttctctc gtatggatc 
ttgtcgctg actttttcc accgttccg atttattgg 
aacacatgg accgaagag aaaacttgt gatgttcag 
ataattccg gaagtgtac tttaatttc gtccttacg 
ccgatgttt gttttgatt tggggtgtg atgctcctt 
gtttatatt aaaatttgg agagaggcc tcaaaacat 
gcgaaaagg attagaagc acgacaaat ttgcataaa 
tgtcacaac atcaacgat actaaatca atacaggtg 
gtgatgctc actctcggc tgcttttcc atctgctgg 
atgccgtac ttcatcgtg accacttat ttccggata 
agcggcagc agcagtcag ttcagcctt ttgtacgaa 
atcgcgttc acgctggcc gtttgcaat tcaggaatg 
aaccctgtg atatacgct tggaagaat tccaacttc 
cgtgaggct ttttggtgt ttgctgact tgccactcg 
ccgaacaat tccaccaaa aagacaaac tacatcacc 
aatcatgtt cccagcagc aagaaaagc tcgataggc 
ggacacgac aacgttgtc gctcaaacc gaaattgag 
atcgaatcg cccacaccc aaagactat gacgagcac 
gagtgcagt gtctcgacc gaaatgacc acagtgaac 
acttccact tggagttga
    mRNA sequence
    • >TC003331 RA
      gtcggttta aaatacaac ttatcgaaa atgtaaatc 
gtgagattt atgctgagt ggccacggg atgcaatta 
aatcaattg aaatgttgc caaacgatt aatcaatga 
tgggagaaa cgtcgaccg aaaattcca ccacagtgt 
ccgattatg taggtggtg gtttgtgga taacagtgt 
actgtataa ttatcgttt gtgctattg ctggcaaca 
ctttgacac ttttcgcaa tagcggcca gccgacagt 
tgtcgtcga tggtgtcca accaattca tcttcagct 
tgggagtga gcgacttat tggtggggc tgtccatcc 
cttaccata tgtgcttct acttgacgg actcaatca 
ttcacaacg aaaccactt gtttggccc gatttgtcc 
tcatatctt tcgcttgtg caagctcga tattaaacc 
ttcttttcg tcgccacgg acagatata cggccatcg 
tccatccct tgaaataca accagtata tgacgcggc 
aaaatgcaa cttttctca tttctctcg tatggatct 
tgtcgctga ctttttcca ccgttccga tttattgga 
acacatgga ccgaagaga aaacttgtg atgttcaga 
taattccgg aagtgtact ttaatttcg tccttacgc 
cgatgtttg ttttgattt ggggtgtga tgctccttg 
tttatatta aaatttgga gagaggcct caaaacatg 
cgaaaagga ttagaagca cgacaaatt tgcataaat 
gtcacaaca tcaacgata ctaaatcaa tacaggtgg 
tgatgctca ctctcggct gcttttcca tctgctgga 
tgccgtact tcatcgtga ccacttatt tccggataa 
gcggcagca gcagtcagt tcagccttt tgtacgaaa 
tcgcgttca cgctggccg tttgcaatt caggaatga 
accctgtga tatacgctt ggaagaatt ccaacttcc 
gtgaggctt tttggtgtt tgctgactt gccactcgc 
cgaacaatt ccaccaaaa agacaaact acatcacca 
atcatgttc ccagcagca agaaaagct cgataggcg 
gacacgaca acgttgtcg ctcaaaccg aaattgaga 
tcgaatcgc ccacaccca aagactatg acgagcacg 
agtgcagtg tctcgaccg aaatgacca cagtgaaca 
cttccactt ggagttgac tttagtttt aattctccg 
acgg