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Gene information
General information
TC034577 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 254836..257834
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034577
    iBeetle screen iB_03539
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen dorsal partiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034577 RA
      MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE 
YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA 
SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH 
GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV 
ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA 
HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA 
VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV 
TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL 
SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES 
RKNRVNERE ARIFWMALV ITPLLWGVF FITALFWLK 
LKWMLLIMI ALALNGANF YGYFKCKVG SSESLSSAT 
TNFLRKQVL QNAVSLVTR EATAPQANP MSQPSNTI
    • >TC034577 RB
      MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE 
YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA 
SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH 
GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV 
ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA 
HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA 
VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV 
TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL 
SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES 
RKGRWQNRV NEREARIFW MALVITPLL WGVFFITAL 
FWLKLKWML LIMIALALN GANFYGYFK CKVGSSESL 
SSATTNFLR KQVLQNAVS LVTREATAP QANPMSQPS 
NTI
    CDS sequence
    • >TC034577 RA
      atggtattg ttctcttta tacataact gtaacagcc 
acgttgtta gtacaaagt tttaatagt gccggaaga 
actaacttt caaatttat gcgaatcct ctccaggag 
tatcaacga attgccttt ccgcaatca aaagatagt 
aacacgaac aaaaatcgc ttaaacttt aacgaaatc 
acacaagcg ctcagtaga ctgcttgaa aaggtcgct 
tctccgatg aaccaacgg aaaagagat acgattatc 
aaagccaaa aaagcttac cggcactta tccaggccc 
cgggaacca agccacaag tcttctgga acaggacac 
ggaaaaaaa cgctgggat agatggacc caatggtcc 
caatgtagc gtaagttgc ggtaaaggc agagaaatc 
cggtggaga cactgcgtg gagaactgt gggggcgtg 
gagacggaa atggaggaa aaagcctgc cagctcccg 
gcctgtgga ggaaaatta tttggaata attaagttg 
acaagtcgt agtcacatt ttggtgaca aactcggca 
cattattat tacaaaaag gaatttatt acgactttc 
ggtttgata acgtttcgg tcccgcgag cagagactt 
gacagctgt cgcgttgac atttgtaat ttattagcc 
gtaaacatg tcatcagcg acgttgcct ttattggag 
gatgacaca gtagcattc ggtgatgat ttaggtgat 
agtggtgtc aaggggtcc ctgacacac ccttatgtc 
acatttttc cacttggcc tttcggggg gcgtctata 
gtaacgtac ttgttgtgc gaatggttc agtgatagt 
tttatcgca agttttgta acggtagtt ttattatta 
tcgatggac ttttggaca gtcaaaaac ataacaggc 
cgtctgatg gttggcttg cgatggtgg aattacgtg 
gacgatgac ggaaagtcg cactgggtc tttgaatca 
cgcaagaac cgtgtaaac gagcgagaa gcgagaata 
ttttggatg gctttggtt ataaccccc ctgttgtgg 
ggcgtgttt ttcataact gctttgttt tggctaaaa 
ctaaagtgg atgctgctc ataatgata gcgttggct 
ttaaatggg gccaatttc tatgggtat tttaaatgt 
aaagtgggt tcaagtgag agcttaagt tccgcaacc 
acaaatttt ctcaggaaa caagtttta caaaatgct 
gtatcactt gttacacgt gaggctacc gcccctcaa 
gcaaatccc atgagccag ccatctaac actatttaa
    • >TC034577 RB
      atggtattg ttctcttta tacataact gtaacagcc 
acgttgtta gtacaaagt tttaatagt gccggaaga 
actaacttt caaatttat gcgaatcct ctccaggag 
tatcaacga attgccttt ccgcaatca aaagatagt 
aacacgaac aaaaatcgc ttaaacttt aacgaaatc 
acacaagcg ctcagtaga ctgcttgaa aaggtcgct 
tctccgatg aaccaacgg aaaagagat acgattatc 
aaagccaaa aaagcttac cggcactta tccaggccc 
cgggaacca agccacaag tcttctgga acaggacac 
ggaaaaaaa cgctgggat agatggacc caatggtcc 
caatgtagc gtaagttgc ggtaaaggc agagaaatc 
cggtggaga cactgcgtg gagaactgt gggggcgtg 
gagacggaa atggaggaa aaagcctgc cagctcccg 
gcctgtgga ggaaaatta tttggaata attaagttg 
acaagtcgt agtcacatt ttggtgaca aactcggca 
cattattat tacaaaaag gaatttatt acgactttc 
ggtttgata acgtttcgg tcccgcgag cagagactt 
gacagctgt cgcgttgac atttgtaat ttattagcc 
gtaaacatg tcatcagcg acgttgcct ttattggag 
gatgacaca gtagcattc ggtgatgat ttaggtgat 
agtggtgtc aaggggtcc ctgacacac ccttatgtc 
acatttttc cacttggcc tttcggggg gcgtctata 
gtaacgtac ttgttgtgc gaatggttc agtgatagt 
tttatcgca agttttgta acggtagtt ttattatta 
tcgatggac ttttggaca gtcaaaaac ataacaggc 
cgtctgatg gttggcttg cgatggtgg aattacgtg 
gacgatgac ggaaagtcg cactgggtc tttgaatca 
cgcaagggc cggtggcag aaccgtgta aacgagcga 
gaagcgaga atattttgg atggctttg gttataacc 
cccctgttg tggggcgtg tttttcata actgctttg 
ttttggcta aaactaaag tggatgctg ctcataatg 
atagcgttg gctttaaat ggggccaat ttctatggg 
tattttaaa tgtaaagtg ggttcaagt gagagctta 
agttccgca accacaaat tttctcagg aaacaagtt 
ttacaaaat gctgtatca cttgttaca cgtgaggct 
accgcccct caagcaaat cccatgagc cagccatct 
aacactatt taa
    mRNA sequence
    • >TC034577 RA
      aatttaaat taattgaaa acaactcgc ccccatttt 
tattcgggt accgaaaat cgagttttg tatcacatt 
cctggtatt tagtttaat ttgtgaaat tgagtactt 
ttaattgca tttcacttc ctttaatca ttgtgtaag 
taggtatat atgctgtat cgagagtgt agaagaaaa 
atataactt gtagtggaa cgagtgggt aagaaaaag 
actgagaga ttaccgcca agcattcct ttttgttac 
gtgatcaaa agtttaata ttttggtgg tagtcgtaa 
catgttgag ttggtttcg cattcactg aatggtcat 
agttttata tgtgtggat tcatataag acttgatgt 
tgagtgatg gctgacaca agttttcca tcggaacac 
ttgtgtagg ccatcatga attacagta ggtacgtct 
tattggttt attaatcaa attacaggg tgcttattg 
acaaaattg atgcacaaa gcattttta ccaatttat 
aagagtttt ctatcttca gaaaaacga gacaaaatt 
ttctaagga ttttatacc ttatttaca cattccttt 
taagtgcct acgaaatgc tgttataac tgcgtcgac 
aatatgaat ttgattgtg ttgttacta tgatttaaa 
ttagaatgt gacaatcgg tttgaaggc taaaacttc 
acttcaaga ctgacattt gaacttttt ctggagaag 
actggactt tgatacatt cagattagt tgaagataa 
gttctgcgg tcttacttt ttagtaaaa ctagtcaaa 
atttctagg tatgttcgt cttactagc atgttaagt 
atctgattg tttgcgcca ttgcgatgt caattttcg 
cgttagctg ttgtaatcg cctggtttt atacatagt 
tagcagaag cgcagctaa ttgtccaaa aattgccat 
cctgtatta actagcgtt ttgtaccca aatgtgcaa 
cgatatact ctaaaaaat agttagtgt ccctcgttt 
tataaaaca tttgaaaca aaaatgtca atctaaatt 
tgtcacaat agtgtactg tgtaatggt attgttctc 
tttatacat aactgtaac agccacgtt gttagtaca 
aagttttaa tagtgccgg aagaactaa ctttcaaat 
ttatgcgaa tcctctcca ggagtatca acgaattgc 
ctttccgca atcaaaaga tagtaacac gaacaaaaa 
tcgcttaaa ctttaacga aatcacaca agcgctcag 
tagactgct tgaaaaggt cgcttctcc gatgaacca 
acggaaaag agatacgat tatcaaagc caaaaaagc 
ttaccggca cttatccag gccccggga accaagcca 
caagtcttc tggaacagg acacggaaa aaaacgctg 
ggatagatg gacccaatg gtcccaatg tagcgtaag 
ttgcggtaa aggcagaga aatccggtg gagacactg 
cgtggagaa ctgtggggg cgtggagac ggaaatgga 
ggaaaaagc ctgccagct cccggcctg tggaggaaa 
attatttgg aataattaa gttgacaag tcgtagtca 
cattttggt gacaaactc ggcacatta ttattacaa 
aaaggaatt tattacgac tttcggttt gataacgtt 
tcggtcccg cgagcagag acttgacag ctgtcgcgt 
tgacatttg taatttatt agccgtaaa catgtcatc 
agcgacgtt gcctttatt ggaggatga cacagtagc 
attcggtga tgatttagg tgatagtgg tgtcaaggg 
gtccctgac acaccctta tgtcacatt tttccactt 
ggcctttcg gggggcgtc tatagtaac gtacttgtt 
gtgcgaatg gttcagtga tagttttat cgcaagttt 
tgtaacggt agttttatt attatcgat ggacttttg 
gacagtcaa aaacataac aggccgtct gatggttgg 
cttgcgatg gtggaatta cgtggacga tgacggaaa 
gtcgcactg ggtctttga atcacgcaa gaaccgtgt 
aaacgagcg agaagcgag aatattttg gatggcttt 
ggttataac ccccctgtt gtggggcgt gtttttcat 
aactgcttt gttttggct aaaactaaa gtggatgct 
gctcataat gatagcgtt ggctttaaa tggggccaa 
tttctatgg gtattttaa atgtaaagt gggttcaag 
tgagagctt aagttccgc aaccacaaa ttttctcag 
gaaacaagt tttacaaaa tgctgtatc acttgttac 
acgtgaggc taccgcccc tcaagcaaa tcccatgag 
ccagccatc taacactat ttaataaat cgaccctcc 
tttatttat aaagtattg tgataatta aacttaatt 
ccacacc
    • >TC034577 RB
      aatttaaat taattgaaa acaactcgc ccccatttt 
tattcgggt accgaaaat cgagttttg tatcacatt 
cctggtatt tagtttaat ttgtgaaat tgagtactt 
ttaattgca tttcacttc ctttaatca ttgtgtaag 
taggtatat atgctgtat cgagagtgt agaagaaaa 
atataactt gtagtggaa cgagtgggt aagaaaaag 
actgagaga ttaccgcca agcattcct ttttgttac 
gtgatcaaa agtttaata ttttggtgg tagtcgtaa 
catgttgag ttggtttcg cattcactg aatggtcat 
agttttata tgtgtggat tcatataag acttgatgt 
tgagtgatg gctgacaca agttttcca tcggaacac 
ttgtgtagg ccatcatga attacagta ggtacgtct 
tattggttt attaatcaa attacaggg tgcttattg 
acaaaattg atgcacaaa gcattttta ccaatttat 
aagagtttt ctatcttca gaaaaacga gacaaaatt 
ttctaagga ttttatacc ttatttaca cattccttt 
taagtgcct acgaaatgc tgttataac tgcgtcgac 
aatatgaat ttgattgtg ttgttacta tgatttaaa 
ttagaatgt gacaatcgg tttgaaggc taaaacttc 
acttcaaga ctgacattt gaacttttt ctggagaag 
actggactt tgatacatt cagattagt tgaagataa 
gttctgcgg tcttacttt ttagtaaaa ctagtcaaa 
atttctagg tatgttcgt cttactagc atgttaagt 
atctgattg tttgcgcca ttgcgatgt caattttcg 
cgttagctg ttgtaatcg cctggtttt atacatagt 
tagcagaag cgcagctaa ttgtccaaa aattgccat 
cctgtatta actagcgtt ttgtaccca aatgtgcaa 
cgatatact ctaaaaaat agttagtgt ccctcgttt 
tataaaaca tttgaaaca aaaatgtca atctaaatt 
tgtcacaat agtgtactg tgtaatggt attgttctc 
tttatacat aactgtaac agccacgtt gttagtaca 
aagttttaa tagtgccgg aagaactaa ctttcaaat 
ttatgcgaa tcctctcca ggagtatca acgaattgc 
ctttccgca atcaaaaga tagtaacac gaacaaaaa 
tcgcttaaa ctttaacga aatcacaca agcgctcag 
tagactgct tgaaaaggt cgcttctcc gatgaacca 
acggaaaag agatacgat tatcaaagc caaaaaagc 
ttaccggca cttatccag gccccggga accaagcca 
caagtcttc tggaacagg acacggaaa aaaacgctg 
ggatagatg gacccaatg gtcccaatg tagcgtaag 
ttgcggtaa aggcagaga aatccggtg gagacactg 
cgtggagaa ctgtggggg cgtggagac ggaaatgga 
ggaaaaagc ctgccagct cccggcctg tggaggaaa 
attatttgg aataattaa gttgacaag tcgtagtca 
cattttggt gacaaactc ggcacatta ttattacaa 
aaaggaatt tattacgac tttcggttt gataacgtt 
tcggtcccg cgagcagag acttgacag ctgtcgcgt 
tgacatttg taatttatt agccgtaaa catgtcatc 
agcgacgtt gcctttatt ggaggatga cacagtagc 
attcggtga tgatttagg tgatagtgg tgtcaaggg 
gtccctgac acaccctta tgtcacatt tttccactt 
ggcctttcg gggggcgtc tatagtaac gtacttgtt 
gtgcgaatg gttcagtga tagttttat cgcaagttt 
tgtaacggt agttttatt attatcgat ggacttttg 
gacagtcaa aaacataac aggccgtct gatggttgg 
cttgcgatg gtggaatta cgtggacga tgacggaaa 
gtcgcactg ggtctttga atcacgcaa gggccggtg 
gcagaaccg tgtaaacga gcgagaagc gagaatatt 
ttggatggc tttggttat aacccccct gttgtgggg 
cgtgttttt cataactgc tttgttttg gctaaaact 
aaagtggat gctgctcat aatgatagc gttggcttt 
aaatggggc caatttcta tgggtattt taaatgtaa 
agtgggttc aagtgagag cttaagttc cgcaaccac 
aaattttct caggaaaca agttttaca aaatgctgt 
atcacttgt tacacgtga ggctaccgc ccctcaagc 
aaatcccat gagccagcc atctaacac tatttaata 
aatcgaccc tcctttatt tataaagta ttgtgataa 
ttaaactta attccacac c