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Gene information
General information
TC034577 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 254836..257834
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034577
    iBeetle screen iB_03539
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen dorsal partiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034577 RA
      MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE 
      YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA 
      SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH 
      GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV 
      ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA 
      HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA 
      VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV 
      TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL 
      SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES 
      RKNRVNERE ARIFWMALV ITPLLWGVF FITALFWLK 
      LKWMLLIMI ALALNGANF YGYFKCKVG SSESLSSAT 
      TNFLRKQVL QNAVSLVTR EATAPQANP MSQPSNTI
    • >TC034577 RB
      MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE 
      YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA 
      SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH 
      GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV 
      ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA 
      HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA 
      VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV 
      TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL 
      SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES 
      RKGRWQNRV NEREARIFW MALVITPLL WGVFFITAL 
      FWLKLKWML LIMIALALN GANFYGYFK CKVGSSESL 
      SSATTNFLR KQVLQNAVS LVTREATAP QANPMSQPS 
      NTI
    CDS sequence
    • >TC034577 RA
      atggtattg ttctcttta tacataact gtaacagcc 
      acgttgtta gtacaaagt tttaatagt gccggaaga 
      actaacttt caaatttat gcgaatcct ctccaggag 
      tatcaacga attgccttt ccgcaatca aaagatagt 
      aacacgaac aaaaatcgc ttaaacttt aacgaaatc 
      acacaagcg ctcagtaga ctgcttgaa aaggtcgct 
      tctccgatg aaccaacgg aaaagagat acgattatc 
      aaagccaaa aaagcttac cggcactta tccaggccc 
      cgggaacca agccacaag tcttctgga acaggacac 
      ggaaaaaaa cgctgggat agatggacc caatggtcc 
      caatgtagc gtaagttgc ggtaaaggc agagaaatc 
      cggtggaga cactgcgtg gagaactgt gggggcgtg 
      gagacggaa atggaggaa aaagcctgc cagctcccg 
      gcctgtgga ggaaaatta tttggaata attaagttg 
      acaagtcgt agtcacatt ttggtgaca aactcggca 
      cattattat tacaaaaag gaatttatt acgactttc 
      ggtttgata acgtttcgg tcccgcgag cagagactt 
      gacagctgt cgcgttgac atttgtaat ttattagcc 
      gtaaacatg tcatcagcg acgttgcct ttattggag 
      gatgacaca gtagcattc ggtgatgat ttaggtgat 
      agtggtgtc aaggggtcc ctgacacac ccttatgtc 
      acatttttc cacttggcc tttcggggg gcgtctata 
      gtaacgtac ttgttgtgc gaatggttc agtgatagt 
      tttatcgca agttttgta acggtagtt ttattatta 
      tcgatggac ttttggaca gtcaaaaac ataacaggc 
      cgtctgatg gttggcttg cgatggtgg aattacgtg 
      gacgatgac ggaaagtcg cactgggtc tttgaatca 
      cgcaagaac cgtgtaaac gagcgagaa gcgagaata 
      ttttggatg gctttggtt ataaccccc ctgttgtgg 
      ggcgtgttt ttcataact gctttgttt tggctaaaa 
      ctaaagtgg atgctgctc ataatgata gcgttggct 
      ttaaatggg gccaatttc tatgggtat tttaaatgt 
      aaagtgggt tcaagtgag agcttaagt tccgcaacc 
      acaaatttt ctcaggaaa caagtttta caaaatgct 
      gtatcactt gttacacgt gaggctacc gcccctcaa 
      gcaaatccc atgagccag ccatctaac actatttaa
    • >TC034577 RB
      atggtattg ttctcttta tacataact gtaacagcc 
      acgttgtta gtacaaagt tttaatagt gccggaaga 
      actaacttt caaatttat gcgaatcct ctccaggag 
      tatcaacga attgccttt ccgcaatca aaagatagt 
      aacacgaac aaaaatcgc ttaaacttt aacgaaatc 
      acacaagcg ctcagtaga ctgcttgaa aaggtcgct 
      tctccgatg aaccaacgg aaaagagat acgattatc 
      aaagccaaa aaagcttac cggcactta tccaggccc 
      cgggaacca agccacaag tcttctgga acaggacac 
      ggaaaaaaa cgctgggat agatggacc caatggtcc 
      caatgtagc gtaagttgc ggtaaaggc agagaaatc 
      cggtggaga cactgcgtg gagaactgt gggggcgtg 
      gagacggaa atggaggaa aaagcctgc cagctcccg 
      gcctgtgga ggaaaatta tttggaata attaagttg 
      acaagtcgt agtcacatt ttggtgaca aactcggca 
      cattattat tacaaaaag gaatttatt acgactttc 
      ggtttgata acgtttcgg tcccgcgag cagagactt 
      gacagctgt cgcgttgac atttgtaat ttattagcc 
      gtaaacatg tcatcagcg acgttgcct ttattggag 
      gatgacaca gtagcattc ggtgatgat ttaggtgat 
      agtggtgtc aaggggtcc ctgacacac ccttatgtc 
      acatttttc cacttggcc tttcggggg gcgtctata 
      gtaacgtac ttgttgtgc gaatggttc agtgatagt 
      tttatcgca agttttgta acggtagtt ttattatta 
      tcgatggac ttttggaca gtcaaaaac ataacaggc 
      cgtctgatg gttggcttg cgatggtgg aattacgtg 
      gacgatgac ggaaagtcg cactgggtc tttgaatca 
      cgcaagggc cggtggcag aaccgtgta aacgagcga 
      gaagcgaga atattttgg atggctttg gttataacc 
      cccctgttg tggggcgtg tttttcata actgctttg 
      ttttggcta aaactaaag tggatgctg ctcataatg 
      atagcgttg gctttaaat ggggccaat ttctatggg 
      tattttaaa tgtaaagtg ggttcaagt gagagctta 
      agttccgca accacaaat tttctcagg aaacaagtt 
      ttacaaaat gctgtatca cttgttaca cgtgaggct 
      accgcccct caagcaaat cccatgagc cagccatct 
      aacactatt taa
    mRNA sequence
    • >TC034577 RA
      aatttaaat taattgaaa acaactcgc ccccatttt 
      tattcgggt accgaaaat cgagttttg tatcacatt 
      cctggtatt tagtttaat ttgtgaaat tgagtactt 
      ttaattgca tttcacttc ctttaatca ttgtgtaag 
      taggtatat atgctgtat cgagagtgt agaagaaaa 
      atataactt gtagtggaa cgagtgggt aagaaaaag 
      actgagaga ttaccgcca agcattcct ttttgttac 
      gtgatcaaa agtttaata ttttggtgg tagtcgtaa 
      catgttgag ttggtttcg cattcactg aatggtcat 
      agttttata tgtgtggat tcatataag acttgatgt 
      tgagtgatg gctgacaca agttttcca tcggaacac 
      ttgtgtagg ccatcatga attacagta ggtacgtct 
      tattggttt attaatcaa attacaggg tgcttattg 
      acaaaattg atgcacaaa gcattttta ccaatttat 
      aagagtttt ctatcttca gaaaaacga gacaaaatt 
      ttctaagga ttttatacc ttatttaca cattccttt 
      taagtgcct acgaaatgc tgttataac tgcgtcgac 
      aatatgaat ttgattgtg ttgttacta tgatttaaa 
      ttagaatgt gacaatcgg tttgaaggc taaaacttc 
      acttcaaga ctgacattt gaacttttt ctggagaag 
      actggactt tgatacatt cagattagt tgaagataa 
      gttctgcgg tcttacttt ttagtaaaa ctagtcaaa 
      atttctagg tatgttcgt cttactagc atgttaagt 
      atctgattg tttgcgcca ttgcgatgt caattttcg 
      cgttagctg ttgtaatcg cctggtttt atacatagt 
      tagcagaag cgcagctaa ttgtccaaa aattgccat 
      cctgtatta actagcgtt ttgtaccca aatgtgcaa 
      cgatatact ctaaaaaat agttagtgt ccctcgttt 
      tataaaaca tttgaaaca aaaatgtca atctaaatt 
      tgtcacaat agtgtactg tgtaatggt attgttctc 
      tttatacat aactgtaac agccacgtt gttagtaca 
      aagttttaa tagtgccgg aagaactaa ctttcaaat 
      ttatgcgaa tcctctcca ggagtatca acgaattgc 
      ctttccgca atcaaaaga tagtaacac gaacaaaaa 
      tcgcttaaa ctttaacga aatcacaca agcgctcag 
      tagactgct tgaaaaggt cgcttctcc gatgaacca 
      acggaaaag agatacgat tatcaaagc caaaaaagc 
      ttaccggca cttatccag gccccggga accaagcca 
      caagtcttc tggaacagg acacggaaa aaaacgctg 
      ggatagatg gacccaatg gtcccaatg tagcgtaag 
      ttgcggtaa aggcagaga aatccggtg gagacactg 
      cgtggagaa ctgtggggg cgtggagac ggaaatgga 
      ggaaaaagc ctgccagct cccggcctg tggaggaaa 
      attatttgg aataattaa gttgacaag tcgtagtca 
      cattttggt gacaaactc ggcacatta ttattacaa 
      aaaggaatt tattacgac tttcggttt gataacgtt 
      tcggtcccg cgagcagag acttgacag ctgtcgcgt 
      tgacatttg taatttatt agccgtaaa catgtcatc 
      agcgacgtt gcctttatt ggaggatga cacagtagc 
      attcggtga tgatttagg tgatagtgg tgtcaaggg 
      gtccctgac acaccctta tgtcacatt tttccactt 
      ggcctttcg gggggcgtc tatagtaac gtacttgtt 
      gtgcgaatg gttcagtga tagttttat cgcaagttt 
      tgtaacggt agttttatt attatcgat ggacttttg 
      gacagtcaa aaacataac aggccgtct gatggttgg 
      cttgcgatg gtggaatta cgtggacga tgacggaaa 
      gtcgcactg ggtctttga atcacgcaa gaaccgtgt 
      aaacgagcg agaagcgag aatattttg gatggcttt 
      ggttataac ccccctgtt gtggggcgt gtttttcat 
      aactgcttt gttttggct aaaactaaa gtggatgct 
      gctcataat gatagcgtt ggctttaaa tggggccaa 
      tttctatgg gtattttaa atgtaaagt gggttcaag 
      tgagagctt aagttccgc aaccacaaa ttttctcag 
      gaaacaagt tttacaaaa tgctgtatc acttgttac 
      acgtgaggc taccgcccc tcaagcaaa tcccatgag 
      ccagccatc taacactat ttaataaat cgaccctcc 
      tttatttat aaagtattg tgataatta aacttaatt 
      ccacacc
    • >TC034577 RB
      aatttaaat taattgaaa acaactcgc ccccatttt 
      tattcgggt accgaaaat cgagttttg tatcacatt 
      cctggtatt tagtttaat ttgtgaaat tgagtactt 
      ttaattgca tttcacttc ctttaatca ttgtgtaag 
      taggtatat atgctgtat cgagagtgt agaagaaaa 
      atataactt gtagtggaa cgagtgggt aagaaaaag 
      actgagaga ttaccgcca agcattcct ttttgttac 
      gtgatcaaa agtttaata ttttggtgg tagtcgtaa 
      catgttgag ttggtttcg cattcactg aatggtcat 
      agttttata tgtgtggat tcatataag acttgatgt 
      tgagtgatg gctgacaca agttttcca tcggaacac 
      ttgtgtagg ccatcatga attacagta ggtacgtct 
      tattggttt attaatcaa attacaggg tgcttattg 
      acaaaattg atgcacaaa gcattttta ccaatttat 
      aagagtttt ctatcttca gaaaaacga gacaaaatt 
      ttctaagga ttttatacc ttatttaca cattccttt 
      taagtgcct acgaaatgc tgttataac tgcgtcgac 
      aatatgaat ttgattgtg ttgttacta tgatttaaa 
      ttagaatgt gacaatcgg tttgaaggc taaaacttc 
      acttcaaga ctgacattt gaacttttt ctggagaag 
      actggactt tgatacatt cagattagt tgaagataa 
      gttctgcgg tcttacttt ttagtaaaa ctagtcaaa 
      atttctagg tatgttcgt cttactagc atgttaagt 
      atctgattg tttgcgcca ttgcgatgt caattttcg 
      cgttagctg ttgtaatcg cctggtttt atacatagt 
      tagcagaag cgcagctaa ttgtccaaa aattgccat 
      cctgtatta actagcgtt ttgtaccca aatgtgcaa 
      cgatatact ctaaaaaat agttagtgt ccctcgttt 
      tataaaaca tttgaaaca aaaatgtca atctaaatt 
      tgtcacaat agtgtactg tgtaatggt attgttctc 
      tttatacat aactgtaac agccacgtt gttagtaca 
      aagttttaa tagtgccgg aagaactaa ctttcaaat 
      ttatgcgaa tcctctcca ggagtatca acgaattgc 
      ctttccgca atcaaaaga tagtaacac gaacaaaaa 
      tcgcttaaa ctttaacga aatcacaca agcgctcag 
      tagactgct tgaaaaggt cgcttctcc gatgaacca 
      acggaaaag agatacgat tatcaaagc caaaaaagc 
      ttaccggca cttatccag gccccggga accaagcca 
      caagtcttc tggaacagg acacggaaa aaaacgctg 
      ggatagatg gacccaatg gtcccaatg tagcgtaag 
      ttgcggtaa aggcagaga aatccggtg gagacactg 
      cgtggagaa ctgtggggg cgtggagac ggaaatgga 
      ggaaaaagc ctgccagct cccggcctg tggaggaaa 
      attatttgg aataattaa gttgacaag tcgtagtca 
      cattttggt gacaaactc ggcacatta ttattacaa 
      aaaggaatt tattacgac tttcggttt gataacgtt 
      tcggtcccg cgagcagag acttgacag ctgtcgcgt 
      tgacatttg taatttatt agccgtaaa catgtcatc 
      agcgacgtt gcctttatt ggaggatga cacagtagc 
      attcggtga tgatttagg tgatagtgg tgtcaaggg 
      gtccctgac acaccctta tgtcacatt tttccactt 
      ggcctttcg gggggcgtc tatagtaac gtacttgtt 
      gtgcgaatg gttcagtga tagttttat cgcaagttt 
      tgtaacggt agttttatt attatcgat ggacttttg 
      gacagtcaa aaacataac aggccgtct gatggttgg 
      cttgcgatg gtggaatta cgtggacga tgacggaaa 
      gtcgcactg ggtctttga atcacgcaa gggccggtg 
      gcagaaccg tgtaaacga gcgagaagc gagaatatt 
      ttggatggc tttggttat aacccccct gttgtgggg 
      cgtgttttt cataactgc tttgttttg gctaaaact 
      aaagtggat gctgctcat aatgatagc gttggcttt 
      aaatggggc caatttcta tgggtattt taaatgtaa 
      agtgggttc aagtgagag cttaagttc cgcaaccac 
      aaattttct caggaaaca agttttaca aaatgctgt 
      atcacttgt tacacgtga ggctaccgc ccctcaagc 
      aaatcccat gagccagcc atctaacac tatttaata 
      aatcgaccc tcctttatt tataaagta ttgtgataa 
      ttaaactta attccacac c