General information | |||
TC034577 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1333347..1336345 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034577 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03539 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen dorsal partiallynot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
remarks: later than schedule,
- >TC034577 RA
MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES RKNRVNERE ARIFWMALV ITPLLWGVF FITALFWLK LKWMLLIMI ALALNGANF YGYFKCKVG SSESLSSAT TNFLRKQVL QNAVSLVTR EATAPQANP MSQPSNTI
- >TC034577 RB
MVLFSLYIT VTATLLVQS FNSAGRTNF QIYANPLQE YQRIAFPQS KDSNTNKNR LNFNEITQA LSRLLEKVA SPMNQRKRD TIIKAKKAY RHLSRPREP SHKSSGTGH GKKRWDRWT QWSQCSVSC GKGREIRWR HCVENCGGV ETEMEEKAC QLPACGGKL FGIIKLTSR SHILVTNSA HYYYKKEFI TTFGLITFR SREQRLDSC RVDICNLLA VNMSSATLP LLEDDTVAF GDDLGDSGV KGSLTHPYV TFFHLAFRG ASIVTYLLC EWFSDSFIA SFVTVVLLL SMDFWTVKN ITGRLMVGL RWWNYVDDD GKSHWVFES RKGRWQNRV NEREARIFW MALVITPLL WGVFFITAL FWLKLKWML LIMIALALN GANFYGYFK CKVGSSESL SSATTNFLR KQVLQNAVS LVTREATAP QANPMSQPS NTI
- >TC034577 RA
atggtattg ttctcttta tacataact gtaacagcc acgttgtta gtacaaagt tttaatagt gccggaaga actaacttt caaatttat gcgaatcct ctccaggag tatcaacga attgccttt ccgcaatca aaagatagt aacacgaac aaaaatcgc ttaaacttt aacgaaatc acacaagcg ctcagtaga ctgcttgaa aaggtcgct tctccgatg aaccaacgg aaaagagat acgattatc aaagccaaa aaagcttac cggcactta tccaggccc cgggaacca agccacaag tcttctgga acaggacac ggaaaaaaa cgctgggat agatggacc caatggtcc caatgtagc gtaagttgc ggtaaaggc agagaaatc cggtggaga cactgcgtg gagaactgt gggggcgtg gagacggaa atggaggaa aaagcctgc cagctcccg gcctgtgga ggaaaatta tttggaata attaagttg acaagtcgt agtcacatt ttggtgaca aactcggca cattattat tacaaaaag gaatttatt acgactttc ggtttgata acgtttcgg tcccgcgag cagagactt gacagctgt cgcgttgac atttgtaat ttattagcc gtaaacatg tcatcagcg acgttgcct ttattggag gatgacaca gtagcattc ggtgatgat ttaggtgat agtggtgtc aaggggtcc ctgacacac ccttatgtc acatttttc cacttggcc tttcggggg gcgtctata gtaacgtac ttgttgtgc gaatggttc agtgatagt tttatcgca agttttgta acggtagtt ttattatta tcgatggac ttttggaca gtcaaaaac ataacaggc cgtctgatg gttggcttg cgatggtgg aattacgtg gacgatgac ggaaagtcg cactgggtc tttgaatca cgcaagaac cgtgtaaac gagcgagaa gcgagaata ttttggatg gctttggtt ataaccccc ctgttgtgg ggcgtgttt ttcataact gctttgttt tggctaaaa ctaaagtgg atgctgctc ataatgata gcgttggct ttaaatggg gccaatttc tatgggtat tttaaatgt aaagtgggt tcaagtgag agcttaagt tccgcaacc acaaatttt ctcaggaaa caagtttta caaaatgct gtatcactt gttacacgt gaggctacc gcccctcaa gcaaatccc atgagccag ccatctaac actatttaa
- >TC034577 RB
atggtattg ttctcttta tacataact gtaacagcc acgttgtta gtacaaagt tttaatagt gccggaaga actaacttt caaatttat gcgaatcct ctccaggag tatcaacga attgccttt ccgcaatca aaagatagt aacacgaac aaaaatcgc ttaaacttt aacgaaatc acacaagcg ctcagtaga ctgcttgaa aaggtcgct tctccgatg aaccaacgg aaaagagat acgattatc aaagccaaa aaagcttac cggcactta tccaggccc cgggaacca agccacaag tcttctgga acaggacac ggaaaaaaa cgctgggat agatggacc caatggtcc caatgtagc gtaagttgc ggtaaaggc agagaaatc cggtggaga cactgcgtg gagaactgt gggggcgtg gagacggaa atggaggaa aaagcctgc cagctcccg gcctgtgga ggaaaatta tttggaata attaagttg acaagtcgt agtcacatt ttggtgaca aactcggca cattattat tacaaaaag gaatttatt acgactttc ggtttgata acgtttcgg tcccgcgag cagagactt gacagctgt cgcgttgac atttgtaat ttattagcc gtaaacatg tcatcagcg acgttgcct ttattggag gatgacaca gtagcattc ggtgatgat ttaggtgat agtggtgtc aaggggtcc ctgacacac ccttatgtc acatttttc cacttggcc tttcggggg gcgtctata gtaacgtac ttgttgtgc gaatggttc agtgatagt tttatcgca agttttgta acggtagtt ttattatta tcgatggac ttttggaca gtcaaaaac ataacaggc cgtctgatg gttggcttg cgatggtgg aattacgtg gacgatgac ggaaagtcg cactgggtc tttgaatca cgcaagggc cggtggcag aaccgtgta aacgagcga gaagcgaga atattttgg atggctttg gttataacc cccctgttg tggggcgtg tttttcata actgctttg ttttggcta aaactaaag tggatgctg ctcataatg atagcgttg gctttaaat ggggccaat ttctatggg tattttaaa tgtaaagtg ggttcaagt gagagctta agttccgca accacaaat tttctcagg aaacaagtt ttacaaaat gctgtatca cttgttaca cgtgaggct accgcccct caagcaaat cccatgagc cagccatct aacactatt taa
- >TC034577 RA
aatttaaat taattgaaa acaactcgc ccccatttt tattcgggt accgaaaat cgagttttg tatcacatt cctggtatt tagtttaat ttgtgaaat tgagtactt ttaattgca tttcacttc ctttaatca ttgtgtaag taggtatat atgctgtat cgagagtgt agaagaaaa atataactt gtagtggaa cgagtgggt aagaaaaag actgagaga ttaccgcca agcattcct ttttgttac gtgatcaaa agtttaata ttttggtgg tagtcgtaa catgttgag ttggtttcg cattcactg aatggtcat agttttata tgtgtggat tcatataag acttgatgt tgagtgatg gctgacaca agttttcca tcggaacac ttgtgtagg ccatcatga attacagta ggtacgtct tattggttt attaatcaa attacaggg tgcttattg acaaaattg atgcacaaa gcattttta ccaatttat aagagtttt ctatcttca gaaaaacga gacaaaatt ttctaagga ttttatacc ttatttaca cattccttt taagtgcct acgaaatgc tgttataac tgcgtcgac aatatgaat ttgattgtg ttgttacta tgatttaaa ttagaatgt gacaatcgg tttgaaggc taaaacttc acttcaaga ctgacattt gaacttttt ctggagaag actggactt tgatacatt cagattagt tgaagataa gttctgcgg tcttacttt ttagtaaaa ctagtcaaa atttctagg tatgttcgt cttactagc atgttaagt atctgattg tttgcgcca ttgcgatgt caattttcg cgttagctg ttgtaatcg cctggtttt atacatagt tagcagaag cgcagctaa ttgtccaaa aattgccat cctgtatta actagcgtt ttgtaccca aatgtgcaa cgatatact ctaaaaaat agttagtgt ccctcgttt tataaaaca tttgaaaca aaaatgtca atctaaatt tgtcacaat agtgtactg tgtaatggt attgttctc tttatacat aactgtaac agccacgtt gttagtaca aagttttaa tagtgccgg aagaactaa ctttcaaat ttatgcgaa tcctctcca ggagtatca acgaattgc ctttccgca atcaaaaga tagtaacac gaacaaaaa tcgcttaaa ctttaacga aatcacaca agcgctcag tagactgct tgaaaaggt cgcttctcc gatgaacca acggaaaag agatacgat tatcaaagc caaaaaagc ttaccggca cttatccag gccccggga accaagcca caagtcttc tggaacagg acacggaaa aaaacgctg ggatagatg gacccaatg gtcccaatg tagcgtaag ttgcggtaa aggcagaga aatccggtg gagacactg cgtggagaa ctgtggggg cgtggagac ggaaatgga ggaaaaagc ctgccagct cccggcctg tggaggaaa attatttgg aataattaa gttgacaag tcgtagtca cattttggt gacaaactc ggcacatta ttattacaa aaaggaatt tattacgac tttcggttt gataacgtt tcggtcccg cgagcagag acttgacag ctgtcgcgt tgacatttg taatttatt agccgtaaa catgtcatc agcgacgtt gcctttatt ggaggatga cacagtagc attcggtga tgatttagg tgatagtgg tgtcaaggg gtccctgac acaccctta tgtcacatt tttccactt ggcctttcg gggggcgtc tatagtaac gtacttgtt gtgcgaatg gttcagtga tagttttat cgcaagttt tgtaacggt agttttatt attatcgat ggacttttg gacagtcaa aaacataac aggccgtct gatggttgg cttgcgatg gtggaatta cgtggacga tgacggaaa gtcgcactg ggtctttga atcacgcaa gaaccgtgt aaacgagcg agaagcgag aatattttg gatggcttt ggttataac ccccctgtt gtggggcgt gtttttcat aactgcttt gttttggct aaaactaaa gtggatgct gctcataat gatagcgtt ggctttaaa tggggccaa tttctatgg gtattttaa atgtaaagt gggttcaag tgagagctt aagttccgc aaccacaaa ttttctcag gaaacaagt tttacaaaa tgctgtatc acttgttac acgtgaggc taccgcccc tcaagcaaa tcccatgag ccagccatc taacactat ttaataaat cgaccctcc tttatttat aaagtattg tgataatta aacttaatt ccacacc
- >TC034577 RB
aatttaaat taattgaaa acaactcgc ccccatttt tattcgggt accgaaaat cgagttttg tatcacatt cctggtatt tagtttaat ttgtgaaat tgagtactt ttaattgca tttcacttc ctttaatca ttgtgtaag taggtatat atgctgtat cgagagtgt agaagaaaa atataactt gtagtggaa cgagtgggt aagaaaaag actgagaga ttaccgcca agcattcct ttttgttac gtgatcaaa agtttaata ttttggtgg tagtcgtaa catgttgag ttggtttcg cattcactg aatggtcat agttttata tgtgtggat tcatataag acttgatgt tgagtgatg gctgacaca agttttcca tcggaacac ttgtgtagg ccatcatga attacagta ggtacgtct tattggttt attaatcaa attacaggg tgcttattg acaaaattg atgcacaaa gcattttta ccaatttat aagagtttt ctatcttca gaaaaacga gacaaaatt ttctaagga ttttatacc ttatttaca cattccttt taagtgcct acgaaatgc tgttataac tgcgtcgac aatatgaat ttgattgtg ttgttacta tgatttaaa ttagaatgt gacaatcgg tttgaaggc taaaacttc acttcaaga ctgacattt gaacttttt ctggagaag actggactt tgatacatt cagattagt tgaagataa gttctgcgg tcttacttt ttagtaaaa ctagtcaaa atttctagg tatgttcgt cttactagc atgttaagt atctgattg tttgcgcca ttgcgatgt caattttcg cgttagctg ttgtaatcg cctggtttt atacatagt tagcagaag cgcagctaa ttgtccaaa aattgccat cctgtatta actagcgtt ttgtaccca aatgtgcaa cgatatact ctaaaaaat agttagtgt ccctcgttt tataaaaca tttgaaaca aaaatgtca atctaaatt tgtcacaat agtgtactg tgtaatggt attgttctc tttatacat aactgtaac agccacgtt gttagtaca aagttttaa tagtgccgg aagaactaa ctttcaaat ttatgcgaa tcctctcca ggagtatca acgaattgc ctttccgca atcaaaaga tagtaacac gaacaaaaa tcgcttaaa ctttaacga aatcacaca agcgctcag tagactgct tgaaaaggt cgcttctcc gatgaacca acggaaaag agatacgat tatcaaagc caaaaaagc ttaccggca cttatccag gccccggga accaagcca caagtcttc tggaacagg acacggaaa aaaacgctg ggatagatg gacccaatg gtcccaatg tagcgtaag ttgcggtaa aggcagaga aatccggtg gagacactg cgtggagaa ctgtggggg cgtggagac ggaaatgga ggaaaaagc ctgccagct cccggcctg tggaggaaa attatttgg aataattaa gttgacaag tcgtagtca cattttggt gacaaactc ggcacatta ttattacaa aaaggaatt tattacgac tttcggttt gataacgtt tcggtcccg cgagcagag acttgacag ctgtcgcgt tgacatttg taatttatt agccgtaaa catgtcatc agcgacgtt gcctttatt ggaggatga cacagtagc attcggtga tgatttagg tgatagtgg tgtcaaggg gtccctgac acaccctta tgtcacatt tttccactt ggcctttcg gggggcgtc tatagtaac gtacttgtt gtgcgaatg gttcagtga tagttttat cgcaagttt tgtaacggt agttttatt attatcgat ggacttttg gacagtcaa aaacataac aggccgtct gatggttgg cttgcgatg gtggaatta cgtggacga tgacggaaa gtcgcactg ggtctttga atcacgcaa gggccggtg gcagaaccg tgtaaacga gcgagaagc gagaatatt ttggatggc tttggttat aacccccct gttgtgggg cgtgttttt cataactgc tttgttttg gctaaaact aaagtggat gctgctcat aatgatagc gttggcttt aaatggggc caatttcta tgggtattt taaatgtaa agtgggttc aagtgagag cttaagttc cgcaaccac aaattttct caggaaaca agttttaca aaatgctgt atcacttgt tacacgtga ggctaccgc ccctcaagc aaatcccat gagccagcc atctaacac tatttaata aatcgaccc tcctttatt tataaagta ttgtgataa ttaaactta attccacac c
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
