Status
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Gene information
General information
TC032303
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003281,TC003282
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 715770..721044
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032303
    iBeetle screen iB_03533

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032303 RA
      MDLDDTPRS VVVTTSRVY SDVFSNKPS SYFEYDNYN 
      PPTDDINNY SLIRKIGQG KYSLVFEGV HDGTNDSVV 
      VKLLKPIKK PKIKREIKI LEHLRNGPN IVSLYAVVS 
      VPSTALHAL IFESPSNNE DFKEVYLKL SDSDIRFYI 
      YEILKALDF CHSKGIMHR DVKPHNIII DRKNRKIRL 
      IDFGLAEFY RPGERYNVR VASRHYKGP ELLVEYGYY 
      DYSLDLWSL GCVFAAMIF RKEPFFQGF DNRNQLYCI 
      VKVLGTSKF YSYLNKYNI VLEGSMQEM LGIHSKKPW 
      QRFVNTENE HLVNQNAFD FLESLLCYD HMERCTAQE 
      ALGHKYFGP VRSSGALPG LDKLKVSGS GQAMRFLIA 
      IIILTCLKS SHSGEIFHV PLNGDGSIS LNWVLDYPT 
      QTVTFEVHL PENFGWFAI GFSDQGAHF PADYCILWK 
      TIKRKIQFE DTWADTTGI IRLDRQQDC QNFKIKRAG 
      NVTKFTFRR KFDTCDFED YVIEDGTTH IVWARGAHP 
      LYKVVGLNI SSPEKEQGM VRVQLLKNT NVKAILPNY 
      VQTLDVFAH EVRVPDKET TYWCHVHKL GEEFKEKHH 
      VYRYEAHIP SSSEGLVHH MEVFHCVAP PNQQIPLYV 
      GNCFAKDRP KETQVCKRV LAAWAMGAP PFTYPEEAG 
      LPLGGPDFN PYVMLEVHY NNPEHKTGF VDSSGIRFH 
      VSSKLKKMD AGVIELGLE YTDKMAIPP GQEAFPLTG 
      YCVSECTAV SLPPEGITI FGSQLHTHL TGVKVYTRH 
      IRDGIELRE LNRDDHYST HFQEIRRLK QPVKVLPGD 
      ALVTRCYYN TQERENITL GGFSITDEM CVNYVHYFP 
      ATQLEVCKS AISDQALST YFNYMKEWE GQKISLHHV 
      ISDNYKSIK WNKMRVQLL SDW
    CDS sequence
    • >TC032303 RA
      atggatttg gacgacaca cctcgtagc gttgttgtt 
      acaacatca cgggtatac tcggacgtg ttctcaaac 
      aagccctct tcctacttt gagtacgac aactacaac 
      cccccgact gacgatatc aacaattac agtttgatt 
      cggaaaatc ggtcaaggg aagtacagt ctagtgttc 
      gagggggtg cacgatggt actaacgat tcggtcgtg 
      gtcaagttg ctaaaaccg atcaaaaaa ccgaaaatc 
      aaacgtgaa attaaaatt ttggagcac ttgaggaac 
      ggccccaac attgtgagt ttatatgcc gtggtgtcg 
      gtgccttcc acagctttg cacgccctg attttcgaa 
      tccccgtca aataatgag gactttaag gaggtttat 
      ttgaaattg agtgattcc gatatccgg ttttacatt 
      tacgaaatt ttgaaagcc cttgatttc tgtcacagc 
      aagggcata atgcatcgt gacgtcaag ccgcacaat 
      atcataatt gaccggaaa aatcgcaaa atccggttg 
      attgatttc ggtttggct gagttttac cgtcccgga 
      gaacgttac aacgtccgg gtggcgtct cggcattac 
      aaaggcccg gaattgttg gtagagtac ggttattac 
      gactattcc ctcgacttg tggtcgctt gggtgcgtt 
      tttgccgca atgatcttc cgtaaagaa cctttcttc 
      caaggattt gataaccgc aaccaactg tattgcatt 
      gttaaagtg ttgggcacg agcaagttt tactcttat 
      ttgaataaa tacaacatt gtgctggaa ggtagcatg 
      caggagatg ttggggatt cactcgaaa aaaccctgg 
      cagagattt gtcaatacg gagaatgag catttagtg 
      aatcagaat gcctttgat tttttggaa tcgttgctt 
      tgttatgat cacatggaa agatgtacg gctcaggag 
      gccttgggg cataagtat ttcgggccg gtgagaagc 
      tcgggggct ttgccaggg ttggataag ctcaaggtt 
      tcagggtcg gggcaagcg atgcgcttt cttatcgca 
      atcataatc ctgacgtgt ctgaaaagt tcacactcc 
      ggtgaaatt ttccacgtc ccgttgaac ggtgatggg 
      tccatttcc ctcaactgg gtcctggat tatcccact 
      caaaccgtc actttcgag gtccacctc ccggaaaat 
      ttcggctgg tttgcgatt ggtttttcc gaccaaggg 
      gcgcatttc ccggccgat tattgcata ctatggaag 
      acaatcaaa cggaaaatt caatttgag gacacgtgg 
      gccgacacc actgggatt atacggctg gaccgccaa 
      caagattgc caaaatttc aaaataaaa cgcgcggga 
      aatgtaacc aagtttacg tttcggcgc aaattcgat 
      acttgcgac tttgaggat tatgttatc gaagatggg 
      acaacacat atcgtgtgg gcacgtggg gcccaccct 
      ttgtataaa gtggtgggg ttgaatatt agctcccct 
      gagaaggag caaggcatg gtgagggtg caattgttg 
      aaaaatacc aatgtgaag gcgattttg cccaattat 
      gtgcaaact ttggatgtt tttgcccat gaagtgagg 
      gttccggac aaggaaacg acgtattgg tgtcacgtc 
      cataaactg ggggaagaa tttaaggag aaacaccat 
      gtttacagg tacgaggcc catattccg agtagtagt 
      gaaggtttg gtccatcat atggaagtg tttcattgt 
      gtggcacct ccgaatcaa caaattccc ctatatgtt 
      ggcaactgt tttgctaaa gacagaccc aaagagacg 
      caagtttgt aaaagagtg ctagctgcg tgggccatg 
      ggggcacca cctttcaca tatccagag gaggccggg 
      cttcccctc ggcggtccc gacttcaac ccctacgtc 
      atgctcgag gtgcattac aacaatccg gagcataaa 
      accggattt gtggatagt tccggaatc cggtttcac 
      gtctcatca aaattgaaa aaaatggac gctggagtt 
      atcgaatta gggttggag tataccgac aaaatggcg 
      attcctcca ggtcaggag gcgtttcct ttgaccgga 
      tattgcgtc agcgagtgt accgctgtg agtttgcca 
      cctgaaggg attacaatt tttgggtca cagttacac 
      actcacctc acaggggtt aaggtgtac actaggcat 
      ataagggat gggattgag ttaagggaa ctgaatagg 
      gatgatcat tacagtacg cattttcag gaaatacgc 
      aggttgaaa caaccggtt aaagtctta ccgggcgat 
      gccttggtt acgcgatgt tattataac acacaagag 
      cgggaaaat atcacactt ggagggttt agtatcaca 
      gatgagatg tgtgttaat tatgttcat tacttccca 
      gccactcaa ttggaggtt tgcaagagc gcaattagt 
      gaccaggct ttaagtact tacttcaac tacatgaaa 
      gaatgggag ggccaaaaa atttcgctc caccacgta 
      atttcagat aattacaaa tcaatcaaa tggaataaa 
      atgcgcgtc caattactg agcgattgg tga
    mRNA sequence
    • >TC032303 RA
      aaatgtcaa aaaaagatt tcaaaaaat ttgtgccaa 
      aaatggatt tggacgaca cacctcgta gcgttgttg 
      ttacaacat cacgggtat actcggacg tgttctcaa 
      acaagccct cttcctact ttgagtacg acaactaca 
      accccccga ctgacgata tcaacaatt acagtttga 
      ttcggaaaa tcggtcaag ggaagtaca gtctagtgt 
      tcgaggggg tgcacgatg gtactaacg attcggtcg 
      tggtcaagt tgctaaaac cgatcaaaa aaccgaaaa 
      tcaaacgtg aaattaaaa ttttggagc acttgagga 
      acggcccca acattgtga gtttatatg ccgtggtgt 
      cggtgcctt ccacagctt tgcacgccc tgattttcg 
      aatccccgt caaataatg aggacttta aggaggttt 
      atttgaaat tgagtgatt ccgatatcc ggttttaca 
      tttacgaaa ttttgaaag cccttgatt tctgtcaca 
      gcaagggca taatgcatc gtgacgtca agccgcaca 
      atatcataa ttgaccgga aaaatcgca aaatccggt 
      tgattgatt tcggtttgg ctgagtttt accgtcccg 
      gagaacgtt acaacgtcc gggtggcgt ctcggcatt 
      acaaaggcc cggaattgt tggtagagt acggttatt 
      acgactatt ccctcgact tgtggtcgc ttgggtgcg 
      tttttgccg caatgatct tccgtaaag aacctttct 
      tccaaggat ttgataacc gcaaccaac tgtattgca 
      ttgttaaag tgttgggca cgagcaagt tttactctt 
      atttgaata aatacaaca ttgtgctgg aaggtagca 
      tgcaggaga tgttgggga ttcactcga aaaaaccct 
      ggcagagat ttgtcaata cggagaatg agcatttag 
      tgaatcaga atgcctttg attttttgg aatcgttgc 
      tttgttatg atcacatgg aaagatgta cggctcagg 
      aggccttgg ggcataagt atttcgggc cggtgagaa 
      gctcggggg ctttgccag ggttggata agctcaagg 
      tttcagggt cggggcaag cgatgcgct ttcttatcg 
      caatcataa tcctgacgt gtctgaaaa gttcacact 
      ccggtgaaa ttttccacg tcccgttga acggtgatg 
      ggtccattt ccctcaact gggtcctgg attatccca 
      ctcaaaccg tcactttcg aggtccacc tcccggaaa 
      atttcggct ggtttgcga ttggttttt ccgaccaag 
      gggcgcatt tcccggccg attattgca tactatgga 
      agacaatca aacggaaaa ttcaatttg aggacacgt 
      gggccgaca ccactggga ttatacggc tggaccgcc 
      aacaagatt gccaaaatt tcaaaataa aacgcgcgg 
      gaaatgtaa ccaagttta cgtttcggc gcaaattcg 
      atacttgcg actttgagg attatgtta tcgaagatg 
      ggacaacac atatcgtgt gggcacgtg gggcccacc 
      ctttgtata aagtggtgg ggttgaata ttagctccc 
      ctgagaagg agcaaggca tggtgaggg tgcaattgt 
      tgaaaaata ccaatgtga aggcgattt tgcccaatt 
      atgtgcaaa ctttggatg tttttgccc atgaagtga 
      gggttccgg acaaggaaa cgacgtatt ggtgtcacg 
      tccataaac tgggggaag aatttaagg agaaacacc 
      atgtttaca ggtacgagg cccatattc cgagtagta 
      gtgaaggtt tggtccatc atatggaag tgtttcatt 
      gtgtggcac ctccgaatc aacaaattc ccctatatg 
      ttggcaact gttttgcta aagacagac ccaaagaga 
      cgcaagttt gtaaaagag tgctagctg cgtgggcca 
      tgggggcac cacctttca catatccag aggaggccg 
      ggcttcccc tcggcggtc ccgacttca acccctacg 
      tcatgctcg aggtgcatt acaacaatc cggagcata 
      aaaccggat ttgtggata gttccggaa tccggtttc 
      acgtctcat caaaattga aaaaaatgg acgctggag 
      ttatcgaat tagggttgg agtataccg acaaaatgg 
      cgattcctc caggtcagg aggcgtttc ctttgaccg 
      gatattgcg tcagcgagt gtaccgctg tgagtttgc 
      cacctgaag ggattacaa tttttgggt cacagttac 
      acactcacc tcacagggg ttaaggtgt acactaggc 
      atataaggg atgggattg agttaaggg aactgaata 
      gggatgatc attacagta cgcattttc aggaaatac 
      gcaggttga aacaaccgg ttaaagtct taccgggcg 
      atgccttgg ttacgcgat gttattata acacacaag 
      agcgggaaa atatcacac ttggagggt ttagtatca 
      cagatgaga tgtgtgtta attatgttc attacttcc 
      cagccactc aattggagg tttgcaaga gcgcaatta 
      gtgaccagg ctttaagta cttacttca actacatga 
      aagaatggg agggccaaa aaatttcgc tccaccacg 
      taatttcag ataattaca aatcaatca aatggaata 
      aaatgcgcg tccaattac tgagcgatt ggtgacaga 
      tttcccggt tattgggaa aacaccccc atcaccccc 
      attttaacg cccctaccc ccaccccca cgaacatgt 
      taaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY