General information | |||
TC003262 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 518710..521746 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003262 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03531 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
gnathal appendages partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs pretarsus bent - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003262 RA
MVGMANMDE HERKVVMEF CHLLEKSKQ LFNGLRDLP QYGHKQWQA YFGRTFDIY TKLWKFQQQ HRQILDTKY GLKRWQIGE IASKIGQLY YHYYLRTSE TNYLNEAYS FYAAIRGRA YYSRAAKED RSELMVKKL RYYARFIVV CLLLRRMKL VRELIVELD RHIADYTST YEPDDQIEW SLVLDEIKN FITADSIVT VLHADTNPI VLSHRLSPL TTPPVEKSQ YMNLTLQEI LIVGSASEQ VKFSELTMD MFRMIQTLE REPQEDYNH IYDASPAPG RVPYGVPGQ KGYIENGER PLRRENPHK YLLYKPTLS QVLVFLASG FKELPANGA LLLYLSSDG CFSTTKHPE DMGYDLGGV LTSSRRDGE HKKQKEAHC LYPGDLYPF TRRPLFVVV DSDNSFVFQ HIPRYFGQP LVTLMSPQD TPPAFQDQQ HNGSLFTLF LHSPLTAFC YCCNLTSIP IHHWERCQS FVDRFVTEA SRLFTRSRV DSSYLQFFG DDFLRLLLL RYVFCDVVL HLHRAFRSR QYRPRCQPP LPEAELLEH PSLQHLVLD LAAHLEVRT HFMDNHEID
- >TC003262 RA
atggtcggt atggcaaat atggacgag cacgagcga aaagtagtg atggagttt tgtcatcta ttggaaaag tccaaacaa ctgttcaac gggttacgg gatttgcca caatacggc cacaaacaa tggcaagct tatttcgga cgaactttc gacatctac acaaaatta tggaaattt caacagcag caccgtcaa attttagat acaaagtat gggcttaaa cgatggcaa atcggagag attgcatct aaaattgga caactttat taccactat tatttgagg accagcgag actaattat ttgaacgaa gcttactca ttctatgct gctatcaga ggacgtgct tactactca agagccgca aaagaagac aggtcagaa ttaatggtg aagaaattg agatattac gcacgtttt atcgtagtt tgcctttta ttacgtcga atgaaacta gtgagagaa cttatagta gaattagat cgacacatt gcagattac acgagtacc tacgaacct gacgatcag attgaatgg agtttggtt ttagatgaa ataaaaaat ttcatcacc gctgattcg atagtcact gtcttgcat gcagacaca aatcccatc gttttatcc catcgatta agccctctg acaacccct ccggtggaa aaatcccaa tacatgaac ctaaccctt caagaaatc ctcatcgta ggcagcgcc tctgaacaa gtcaagttt tccgaatta accatggac atgttccgt atgattcaa acccttgaa cgcgaacct caggaagat tacaatcac atctacgac gcgtctcca gctccaggt cgtgtccct tatggcgtt cccggccaa aagggctac attgagaac ggcgaaaga cctctacga cgcgaaaat ccacacaag taccttctg tacaaaccg acacttagc caagttctt gtgtttttg gctagcggc ttcaaggag ttgccagca aatggagca ctgttgttg tatttgtcg tccgatggg tgtttttcc accaccaag catccggag gatatgggt tatgatctt gggggcgtt ttgacgagc agtcggaga gatggagag cacaagaaa cagaaggaa gctcactgc ctttatcct ggtgatttg tatccgttc accaggcga ccgctgttt gttgtcgtt gattcggac aatagtttt gtttttcaa cacattccg aggtatttt ggacagccg ctggttacg ctaatgtcg ccgcaagac acaccaccg gccttccaa gatcaacaa cacaacggt tctttattc actctgttc ctgcactcg cctctgacc gcattctgt tactgttgc aacttgaca agcattccg atccaccac tgggagcgt tgtcagagt ttcgtcgat cgcttcgtc accgaagcc tcccgcctt ttcacacgt tcaagagtc gattcgtca tatcttcaa ttcttcggt gacgatttc ctgcgcctc ctcctcctc cgttacgta ttctgtgac gtggtgctg cacctccac cgtgcgttt cgcagccga cagtatcgc ccccgttgc cagccaccg ctcccagaa gccgaactc ctagagcac ccctccctc cagcacctg gtgctcgat ctggctgca catctcgaa gtgcgcacc cattttatg gacaaccac gaaatcgac tga
- >TC003262 RA
gatgtctgc tgttcgatt tttcgacga cgaccgtcg ttcttttcc accagtgcg taatccggt caaaactaa aagcatgcc cctctgaaa cgaagaaat catggtcgg tatggcaaa tatggacga gcacgagcg aaaagtagt gatggagtt ttgtcatct attggaaaa gtccaaaca actgttcaa cgggttacg ggatttgcc acaatacgg ccacaaaca atggcaagc ttatttcgg acgaacttt cgacatcta cacaaaatt atggaaatt tcaacagca gcaccgtca aattttaga tacaaagta tgggcttaa acgatggca aatcggaga gattgcatc taaaattgg acaacttta ttaccacta ttatttgag gaccagcga gactaatta tttgaacga agcttactc attctatgc tgctatcag aggacgtgc ttactactc aagagccgc aaaagaaga caggtcaga attaatggt gaagaaatt gagatatta cgcacgttt tatcgtagt ttgcctttt attacgtcg aatgaaact agtgagaga acttatagt agaattaga tcgacacat tgcagatta cacgagtac ctacgaacc tgacgatca gattgaatg gagtttggt tttagatga aataaaaaa tttcatcac cgctgattc gatagtcac tgtcttgca tgcagacac aaatcccat cgttttatc ccatcgatt aagccctct gacaacccc tccggtgga aaaatccca atacatgaa cctaaccct tcaagaaat cctcatcgt aggcagcgc ctctgaaca agtcaagtt ttccgaatt aaccatgga catgttccg tatgattca aacccttga acgcgaacc tcaggaaga ttacaatca catctacga cgcgtctcc agctccagg tcgtgtccc ttatggcgt tcccggcca aaagggcta cattgagaa cggcgaaag acctctacg acgcgaaaa tccacacaa gtaccttct gtacaaacc gacacttag ccaagttct tgtgttttt ggctagcgg cttcaagga gttgccagc aaatggagc actgttgtt gtatttgtc gtccgatgg gtgtttttc caccaccaa gcatccgga ggatatggg ttatgatct tgggggcgt tttgacgag cagtcggag agatggaga gcacaagaa acagaagga agctcactg cctttatcc tggtgattt gtatccgtt caccaggcg accgctgtt tgttgtcgt tgattcgga caatagttt tgtttttca acacattcc gaggtattt tggacagcc gctggttac gctaatgtc gccgcaaga cacaccacc ggccttcca agatcaaca acacaacgg ttctttatt cactctgtt cctgcactc gcctctgac cgcattctg ttactgttg caacttgac aagcattcc gatccacca ctgggagcg ttgtcagag tttcgtcga tcgcttcgt caccgaagc ctcccgcct tttcacacg ttcaagagt cgattcgtc atatcttca attcttcgg tgacgattt cctgcgcct cctcctcct ccgttacgt attctgtga cgtggtgct gcacctcca ccgtgcgtt tcgcagccg acagtatcg cccccgttg ccagccacc gctcccaga agccgaact cctagagca cccctccct ccagcacct ggtgctcga tctggctgc acatctcga agtgcgcac ccattttat ggacaacca cgaaatcga ctgatccag ggcttcctc ctcatggcc gtgcgtggg gtttagtgc cacttcgag gaggcacac ccatgagga tgggccccg tggccccct tcttctgct gatcgcctt cgcagccgt gtttttggt cacaaacgc gcacttctc ctcgtggat gatggaagc tttaccatt tttttggac tcttttcga attcgcgac atgtaattt tcggccagt tttggcgac ggctctgtg gctgaaatt gtcgtattt ttggacacc ttcttcaac gttcaagtt tagttcatc ggtgataat atagctcaa agtcgagca cagcgtctt tcaatatat ccgttcact aggcgggcc cttcggtac gaggctcga cggttggac cttcaactg cgttttaac attgccact tttacccaa cttataata ttgtataaa attagtcct ctattgaag ccgtgacca acagtgatt aatacgcct ttcgtgccg tgtacgata cgcattact tattataac taaatttta attactatt gaatgcatg actagatcc ttagaacag gtaccgatc atcacatag tcaattctt tcacacaca aaaattgta ccaccagta cggacctga acaactcac tttttctca tccaaaaca agacaaaac caccaatat aaaaaattt acgttacaa aataccaaa atttcgtta acacagttt agagaaata taaaattta gaaaaaaaa tcctaaaga gacaaaaag cgacgtcga taatataat tttacttga attaaatca ccaattttg ac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
