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Gene information
General information
TC003262 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 518710..521746
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003262
    iBeetle screen iB_03531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    gnathal appendages partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs pretarsus bent - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003262 RA
      MVGMANMDE HERKVVMEF CHLLEKSKQ LFNGLRDLP 
      QYGHKQWQA YFGRTFDIY TKLWKFQQQ HRQILDTKY 
      GLKRWQIGE IASKIGQLY YHYYLRTSE TNYLNEAYS 
      FYAAIRGRA YYSRAAKED RSELMVKKL RYYARFIVV 
      CLLLRRMKL VRELIVELD RHIADYTST YEPDDQIEW 
      SLVLDEIKN FITADSIVT VLHADTNPI VLSHRLSPL 
      TTPPVEKSQ YMNLTLQEI LIVGSASEQ VKFSELTMD 
      MFRMIQTLE REPQEDYNH IYDASPAPG RVPYGVPGQ 
      KGYIENGER PLRRENPHK YLLYKPTLS QVLVFLASG 
      FKELPANGA LLLYLSSDG CFSTTKHPE DMGYDLGGV 
      LTSSRRDGE HKKQKEAHC LYPGDLYPF TRRPLFVVV 
      DSDNSFVFQ HIPRYFGQP LVTLMSPQD TPPAFQDQQ 
      HNGSLFTLF LHSPLTAFC YCCNLTSIP IHHWERCQS 
      FVDRFVTEA SRLFTRSRV DSSYLQFFG DDFLRLLLL 
      RYVFCDVVL HLHRAFRSR QYRPRCQPP LPEAELLEH 
      PSLQHLVLD LAAHLEVRT HFMDNHEID
    CDS sequence
    • >TC003262 RA
      atggtcggt atggcaaat atggacgag cacgagcga 
      aaagtagtg atggagttt tgtcatcta ttggaaaag 
      tccaaacaa ctgttcaac gggttacgg gatttgcca 
      caatacggc cacaaacaa tggcaagct tatttcgga 
      cgaactttc gacatctac acaaaatta tggaaattt 
      caacagcag caccgtcaa attttagat acaaagtat 
      gggcttaaa cgatggcaa atcggagag attgcatct 
      aaaattgga caactttat taccactat tatttgagg 
      accagcgag actaattat ttgaacgaa gcttactca 
      ttctatgct gctatcaga ggacgtgct tactactca 
      agagccgca aaagaagac aggtcagaa ttaatggtg 
      aagaaattg agatattac gcacgtttt atcgtagtt 
      tgcctttta ttacgtcga atgaaacta gtgagagaa 
      cttatagta gaattagat cgacacatt gcagattac 
      acgagtacc tacgaacct gacgatcag attgaatgg 
      agtttggtt ttagatgaa ataaaaaat ttcatcacc 
      gctgattcg atagtcact gtcttgcat gcagacaca 
      aatcccatc gttttatcc catcgatta agccctctg 
      acaacccct ccggtggaa aaatcccaa tacatgaac 
      ctaaccctt caagaaatc ctcatcgta ggcagcgcc 
      tctgaacaa gtcaagttt tccgaatta accatggac 
      atgttccgt atgattcaa acccttgaa cgcgaacct 
      caggaagat tacaatcac atctacgac gcgtctcca 
      gctccaggt cgtgtccct tatggcgtt cccggccaa 
      aagggctac attgagaac ggcgaaaga cctctacga 
      cgcgaaaat ccacacaag taccttctg tacaaaccg 
      acacttagc caagttctt gtgtttttg gctagcggc 
      ttcaaggag ttgccagca aatggagca ctgttgttg 
      tatttgtcg tccgatggg tgtttttcc accaccaag 
      catccggag gatatgggt tatgatctt gggggcgtt 
      ttgacgagc agtcggaga gatggagag cacaagaaa 
      cagaaggaa gctcactgc ctttatcct ggtgatttg 
      tatccgttc accaggcga ccgctgttt gttgtcgtt 
      gattcggac aatagtttt gtttttcaa cacattccg 
      aggtatttt ggacagccg ctggttacg ctaatgtcg 
      ccgcaagac acaccaccg gccttccaa gatcaacaa 
      cacaacggt tctttattc actctgttc ctgcactcg 
      cctctgacc gcattctgt tactgttgc aacttgaca 
      agcattccg atccaccac tgggagcgt tgtcagagt 
      ttcgtcgat cgcttcgtc accgaagcc tcccgcctt 
      ttcacacgt tcaagagtc gattcgtca tatcttcaa 
      ttcttcggt gacgatttc ctgcgcctc ctcctcctc 
      cgttacgta ttctgtgac gtggtgctg cacctccac 
      cgtgcgttt cgcagccga cagtatcgc ccccgttgc 
      cagccaccg ctcccagaa gccgaactc ctagagcac 
      ccctccctc cagcacctg gtgctcgat ctggctgca 
      catctcgaa gtgcgcacc cattttatg gacaaccac 
      gaaatcgac tga
    mRNA sequence
    • >TC003262 RA
      gatgtctgc tgttcgatt tttcgacga cgaccgtcg 
      ttcttttcc accagtgcg taatccggt caaaactaa 
      aagcatgcc cctctgaaa cgaagaaat catggtcgg 
      tatggcaaa tatggacga gcacgagcg aaaagtagt 
      gatggagtt ttgtcatct attggaaaa gtccaaaca 
      actgttcaa cgggttacg ggatttgcc acaatacgg 
      ccacaaaca atggcaagc ttatttcgg acgaacttt 
      cgacatcta cacaaaatt atggaaatt tcaacagca 
      gcaccgtca aattttaga tacaaagta tgggcttaa 
      acgatggca aatcggaga gattgcatc taaaattgg 
      acaacttta ttaccacta ttatttgag gaccagcga 
      gactaatta tttgaacga agcttactc attctatgc 
      tgctatcag aggacgtgc ttactactc aagagccgc 
      aaaagaaga caggtcaga attaatggt gaagaaatt 
      gagatatta cgcacgttt tatcgtagt ttgcctttt 
      attacgtcg aatgaaact agtgagaga acttatagt 
      agaattaga tcgacacat tgcagatta cacgagtac 
      ctacgaacc tgacgatca gattgaatg gagtttggt 
      tttagatga aataaaaaa tttcatcac cgctgattc 
      gatagtcac tgtcttgca tgcagacac aaatcccat 
      cgttttatc ccatcgatt aagccctct gacaacccc 
      tccggtgga aaaatccca atacatgaa cctaaccct 
      tcaagaaat cctcatcgt aggcagcgc ctctgaaca 
      agtcaagtt ttccgaatt aaccatgga catgttccg 
      tatgattca aacccttga acgcgaacc tcaggaaga 
      ttacaatca catctacga cgcgtctcc agctccagg 
      tcgtgtccc ttatggcgt tcccggcca aaagggcta 
      cattgagaa cggcgaaag acctctacg acgcgaaaa 
      tccacacaa gtaccttct gtacaaacc gacacttag 
      ccaagttct tgtgttttt ggctagcgg cttcaagga 
      gttgccagc aaatggagc actgttgtt gtatttgtc 
      gtccgatgg gtgtttttc caccaccaa gcatccgga 
      ggatatggg ttatgatct tgggggcgt tttgacgag 
      cagtcggag agatggaga gcacaagaa acagaagga 
      agctcactg cctttatcc tggtgattt gtatccgtt 
      caccaggcg accgctgtt tgttgtcgt tgattcgga 
      caatagttt tgtttttca acacattcc gaggtattt 
      tggacagcc gctggttac gctaatgtc gccgcaaga 
      cacaccacc ggccttcca agatcaaca acacaacgg 
      ttctttatt cactctgtt cctgcactc gcctctgac 
      cgcattctg ttactgttg caacttgac aagcattcc 
      gatccacca ctgggagcg ttgtcagag tttcgtcga 
      tcgcttcgt caccgaagc ctcccgcct tttcacacg 
      ttcaagagt cgattcgtc atatcttca attcttcgg 
      tgacgattt cctgcgcct cctcctcct ccgttacgt 
      attctgtga cgtggtgct gcacctcca ccgtgcgtt 
      tcgcagccg acagtatcg cccccgttg ccagccacc 
      gctcccaga agccgaact cctagagca cccctccct 
      ccagcacct ggtgctcga tctggctgc acatctcga 
      agtgcgcac ccattttat ggacaacca cgaaatcga 
      ctgatccag ggcttcctc ctcatggcc gtgcgtggg 
      gtttagtgc cacttcgag gaggcacac ccatgagga 
      tgggccccg tggccccct tcttctgct gatcgcctt 
      cgcagccgt gtttttggt cacaaacgc gcacttctc 
      ctcgtggat gatggaagc tttaccatt tttttggac 
      tcttttcga attcgcgac atgtaattt tcggccagt 
      tttggcgac ggctctgtg gctgaaatt gtcgtattt 
      ttggacacc ttcttcaac gttcaagtt tagttcatc 
      ggtgataat atagctcaa agtcgagca cagcgtctt 
      tcaatatat ccgttcact aggcgggcc cttcggtac 
      gaggctcga cggttggac cttcaactg cgttttaac 
      attgccact tttacccaa cttataata ttgtataaa 
      attagtcct ctattgaag ccgtgacca acagtgatt 
      aatacgcct ttcgtgccg tgtacgata cgcattact 
      tattataac taaatttta attactatt gaatgcatg 
      actagatcc ttagaacag gtaccgatc atcacatag 
      tcaattctt tcacacaca aaaattgta ccaccagta 
      cggacctga acaactcac tttttctca tccaaaaca 
      agacaaaac caccaatat aaaaaattt acgttacaa 
      aataccaaa atttcgtta acacagttt agagaaata 
      taaaattta gaaaaaaaa tcctaaaga gacaaaaag 
      cgacgtcga taatataat tttacttga attaaatca 
      ccaattttg ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY