Status
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Gene information
General information
TC003258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 466691..470856
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC003258
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03530

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
      Days post injection

      Fertility
      egg production: number no/almost no eggs -
      tropharium size decreased - Number of animals showing the phenotype: 4

      vitellarium size decreased - Number of animals showing the phenotype: 4
      egg chamber mostly not present - Number of animals showing the phenotype: 4
      Comment: only found one previtellogenic egg chamber in one ovariole
      ovary size decreased - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003258 RA
        MEDRPLSPI LSTQISRKW IRSKRSQKK SRVNLTNRF 
LDASSSNVL TPSQTENFL ESIEKDWSL DKNSDDVLK 
RAEILTPPQ GFNCKNEPN KVDNQGFKA SDEALKKAR 
TLFDDMLNP EGCNKELST GFAGFKTAS GKNSLRSEN 
AVKEARNRN SNEGTTTSF VGFKTASGK SSLMSKSAI 
KQARNKLSE DFNTKISEY NASFSGFKT ASGKCTVMS 
ERAINQARN KLNENFNFE DVKNAKKSD LSDEFRGFS 
QDMIEKNRK KLDEFRSIV TQFNKGVKK EKNLETVRE 
FPKNRGFQS VNLSKDRLE KARQLFQGL DDSFGRKRR 
FFEQEKFGF SPVRKNPSV YNSTPLKNT SFTCLESDV 
TPIKKIKNE TIITQNDRF VIQSCTKGN LDTWLQNLQ 
NERKILEVK LKVIIEKEK VLNLQKEIL ENRVQRQSG 
ILFTRKENS ERVLLKHGI NIDSCRVSS EINSENSPN 
VHLKEKLPM IKTLDGACV VPNVDNFVG LSEIEVAFN 
TIPGVETKL IPNGWVQNH FRWIVWKLA CYENFNFDC 
LTVENIMQQ LKYRYDREI DRAQRPALR KIYETDDTP 
QKRMVLCVA NIFNLGLNK FELELTDGW YSIRTLIDT 
PLCQQIANG KIQIGTKLV IFGAELLNC DGCHPLEAP 
DSVRLKIHC NSTRRALWH AKLGYQKCP DPCLLKLAS 
FYPNGGLIG CIKVYIARV YPLKYLEKQ NTHSVWRNQ 
AAEDLRVIE WESHIRDNL QKMEETIRK DYEMNRTKS 
KKSNVSVNY SEIRNIHCP ETLFNICSN CNDPEGLQE 
LLSDSQKKS IVSYQEQIS LTEQVEISR KIEDSINKH 
ETTKRDVTA LLTLLVLDA HDPSQDKSY VLKIWKPTS 
AHLQMFKES TTCHIYNVS LKKIGVLSA TNWTKFKPT 
DYSEEHFTK YNRFLSPIS TIKPQFNEF DTVGVVVQI 
TVNSFDQNL WLADNCTNL LFVKTFNGP KSCLLFDKL 
KVGQVVAIT NLILFDKTT DFAQAEANH LTVVSSAPR 
HKYLQEGLD LLRQQLPKE LDTFLQECN QKVAFCTVK 
SDITKNYKN SSKSLLQTS DIFDDNLDS FYSTIDFDK 
LK
      CDS sequence
      • >TC003258 RA
        atggaagac cggccttta tcacctatt ctaagcact 
caaatatca cgcaaatgg attagaagc aagagatca 
cagaaaaaa agtcgggtt aatttgacg aaccggttt 
ctggacgcg tcttccagc aatgtttta accccaagc 
caaaccgaa aactttttg gaaagtata gaaaaagac 
tggagtttg gataaaaat tctgatgat gtgttgaaa 
agagccgaa attttgact cctccccaa ggttttaat 
tgtaaaaat gaacccaac aaagttgat aatcaaggt 
ttcaaagcg tctgatgaa gccttgaaa aaggccaga 
acgttgttt gacgacatg ctaaatcct gaaggttgt 
aacaaggaa ttaagtact ggttttgca ggttttaaa 
acagcttca ggaaaaaat tccttaaga tccgaaaat 
gcagtcaaa gaagctagg aataggaac agtaatgag 
ggaactacc acaagtttt gtcggtttt aaaacagct 
tcagggaaa agttcccta atgtctaag agtgcaatc 
aaacaagct aggaataaa ttaagcgaa gattttaat 
acgaaaatt tcggaatat aacgcaagt ttttcgggt 
tttaagaca gcttcaggg aaatgtaca gtaatgtcc 
gaaagagca atcaaccag gccaggaat aaattaaac 
gagaatttt aattttgaa gacgttaaa aatgcgaaa 
aaatcagat ttgagtgac gagttcaga ggttttagt 
caagatatg attgagaaa aatcggaaa aaacttgac 
gaatttcgc tcgattgta acacaattt aacaaaggt 
gtaaaaaaa gaaaaaaac ttagaaacc gttcgggaa 
tttcctaaa aataggggc ttccaatca gtgaattta 
tcaaaagac aggctggaa aaagcgcgt caattgttc 
caaggtctg gacgattcg tttggtcgc aaaaggcgt 
ttttttgag caagaaaaa tttggtttc tctcctgtt 
agaaaaaat ccgagtgtt tacaactca acgcctttg 
aaaaacacc tcttttacg tgtctagaa tcggacgta 
acaccgatc aaaaaaatc aaaaacgag acaattatt 
acacaaaat gatagattt gtaattcag agctgcact 
aaaggaaat ctcgatacg tggcttcaa aatttgcaa 
aacgaacga aaaattctt gaagtaaaa cttaaagtt 
atcatcgag aaggagaaa gttttaaat ttgcaaaaa 
gaaattctg gaaaatagg gttcagaga cagtctgga 
attttattt actagaaaa gaaaatagc gaacgtgtt 
ttgttaaaa catggcata aacatcgat tcgtgtcgt 
gtttcaagt gaaattaat tcggaaaat tcgcccaat 
gttcatttg aaggaaaaa ctgccaatg attaaaacg 
ttagatggt gcttgtgtt gttccaaat gtcgataat 
ttcgttggt ttaagtgag attgaagta gcgtttaat 
acaatacca ggagtcgaa accaaatta ataccaaat 
ggttgggtg caaaatcat ttcagatgg attgtttgg 
aagttggcg tgttacgaa aattttaat ttcgattgt 
ttaacagtc gaaaatatt atgcaacaa ttgaaatat 
cgatatgat cgtgaaatt gatcgggcg caacgtcca 
gcacttcga aaaatttat gaaacggac gatacgccc 
caaaaacga atggtttta tgtgtggct aatattttt 
aacttgggt ttaaataaa ttcgaactg gaattgact 
gacggttgg tatagcatt cgaacgtta attgataca 
cctttatgc cagcaaatt gctaatggg aaaattcaa 
attggtacc aaattggtg atttttggt gcggaattg 
ttgaactgt gatggatgc catccatta gaggcgcct 
gattcagtc cgtttaaaa atccactgc aattcaacg 
agaagagcc ctctggcat gccaaactc ggttatcaa 
aagtgtcca gatccgtgc ttgctaaaa ttggcctcg 
ttttatcca aatgggggc ctcatcggt tgtatcaaa 
gtgtacata gctcgagta tatcctctg aaatatctc 
gaaaaacaa aatacacat tctgtttgg cgaaatcaa 
gcagctgaa gatttacgt gtaatagaa tgggagagt 
cacattcga gataatttg caaaaaatg gaagaaaca 
ataaggaaa gattacgaa atgaaccga acaaaatcg 
aaaaaatca aatgtcagt gtgaattat tccgaaatt 
agaaatatc cattgtcct gaaacgtta ttcaatatt 
tgtagtaat tgtaatgat cctgagggt ctacaggaa 
ttgttatca gattcgcaa aaaaagtca attgtgagt 
taccaggag caaatttca ttgaccgaa caagtggaa 
atatcccgt aaaattgaa gacagtata aataaacat 
gaaacgacc aaaagagac gtaactgca ctgttaaca 
cttttggta ttagatgct cacgatcct tcacaagac 
aaaagttac gtcttaaaa atttggaaa ccaacaagc 
gctcattta caaatgttt aaggaatca acaacttgt 
cacatttac aacgtttcg ttgaaaaaa attggtgtt 
ttgtccgca acgaattgg accaaattc aaaccaact 
gattattcg gaagaacat tttacgaaa tacaaccga 
ttcctttct ccaatctct acaatcaag ccacaattt 
aacgaattt gatacagtg ggtgttgtt gtacaaata 
actgtgaat agttttgat caaaacttg tggttggca 
gataattgc accaatttg ttgtttgtt aaaactttc 
aatggtccg aaaagttgt ctacttttt gataaactc 
aaagttgga caagttgtt gcaattacg aatttaatt 
ttattcgat aagacaaca gattttgca caagctgaa 
gctaatcat ttgacggtc gtttcatcg gcacccaga 
cacaagtat ttgcaagag gggcttgat ttattgcgt 
caacaatta cccaaagaa ttggacact tttttgcaa 
gagtgcaac cagaaagtg gctttttgt accgttaaa 
agtgatata acgaaaaat tacaaaaat tcgtcaaaa 
tctctgcta cagacgtcc gatattttt gatgataat 
ttggatagt ttctactcc acgattgac ttcgataaa 
ttgaagtga
      mRNA sequence
      • >TC003258 RA
        ttgttgatg tgtaatggc ggtgtttac tgtttgtaa 
aaaataaaa atttggtgt ttgttgaaa acgttagtg 
aattttggg caagtttgt gaaaattcg gttttccaa 
cctcaaaat ggaagaccg gcctttatc acctattct 
aagcactca aatatcacg caaatggat tagaagcaa 
gagatcaca gaaaaaaag tcgggttaa tttgacgaa 
ccggtttct ggacgcgtc ttccagcaa tgttttaac 
cccaagcca aaccgaaaa ctttttgga aagtataga 
aaaagactg gagtttgga taaaaattc tgatgatgt 
gttgaaaag agccgaaat tttgactcc tccccaagg 
ttttaattg taaaaatga acccaacaa agttgataa 
tcaaggttt caaagcgtc tgatgaagc cttgaaaaa 
ggccagaac gttgtttga cgacatgct aaatcctga 
aggttgtaa caaggaatt aagtactgg ttttgcagg 
ttttaaaac agcttcagg aaaaaattc cttaagatc 
cgaaaatgc agtcaaaga agctaggaa taggaacag 
taatgaggg aactaccac aagttttgt cggttttaa 
aacagcttc agggaaaag ttccctaat gtctaagag 
tgcaatcaa acaagctag gaataaatt aagcgaaga 
ttttaatac gaaaatttc ggaatataa cgcaagttt 
ttcgggttt taagacagc ttcagggaa atgtacagt 
aatgtccga aagagcaat caaccaggc caggaataa 
attaaacga gaattttaa ttttgaaga cgttaaaaa 
tgcgaaaaa atcagattt gagtgacga gttcagagg 
ttttagtca agatatgat tgagaaaaa tcggaaaaa 
acttgacga atttcgctc gattgtaac acaatttaa 
caaaggtgt aaaaaaaga aaaaaactt agaaaccgt 
tcgggaatt tcctaaaaa taggggctt ccaatcagt 
gaatttatc aaaagacag gctggaaaa agcgcgtca 
attgttcca aggtctgga cgattcgtt tggtcgcaa 
aaggcgttt ttttgagca agaaaaatt tggtttctc 
tcctgttag aaaaaatcc gagtgttta caactcaac 
gcctttgaa aaacacctc ttttacgtg tctagaatc 
ggacgtaac accgatcaa aaaaatcaa aaacgagac 
aattattac acaaaatga tagatttgt aattcagag 
ctgcactaa aggaaatct cgatacgtg gcttcaaaa 
tttgcaaaa cgaacgaaa aattcttga agtaaaact 
taaagttat catcgagaa ggagaaagt tttaaattt 
gcaaaaaga aattctgga aaatagggt tcagagaca 
gtctggaat tttatttac tagaaaaga aaatagcga 
acgtgtttt gttaaaaca tggcataaa catcgattc 
gtgtcgtgt ttcaagtga aattaattc ggaaaattc 
gcccaatgt tcatttgaa ggaaaaact gccaatgat 
taaaacgtt agatggtgc ttgtgttgt tccaaatgt 
cgataattt cgttggttt aagtgagat tgaagtagc 
gtttaatac aataccagg agtcgaaac caaattaat 
accaaatgg ttgggtgca aaatcattt cagatggat 
tgtttggaa gttggcgtg ttacgaaaa ttttaattt 
cgattgttt aacagtcga aaatattat gcaacaatt 
gaaatatcg atatgatcg tgaaattga tcgggcgca 
acgtccagc acttcgaaa aatttatga aacggacga 
tacgcccca aaaacgaat ggttttatg tgtggctaa 
tatttttaa cttgggttt aaataaatt cgaactgga 
attgactga cggttggta tagcattcg aacgttaat 
tgatacacc tttatgcca gcaaattgc taatgggaa 
aattcaaat tggtaccaa attggtgat ttttggtgc 
ggaattgtt gaactgtga tggatgcca tccattaga 
ggcgcctga ttcagtccg tttaaaaat ccactgcaa 
ttcaacgag aagagccct ctggcatgc caaactcgg 
ttatcaaaa gtgtccaga tccgtgctt gctaaaatt 
ggcctcgtt ttatccaaa tgggggcct catcggttg 
tatcaaagt gtacatagc tcgagtata tcctctgaa 
atatctcga aaaacaaaa tacacattc tgtttggcg 
aaatcaagc agctgaaga tttacgtgt aatagaatg 
ggagagtca cattcgaga taatttgca aaaaatgga 
agaaacaat aaggaaaga ttacgaaat gaaccgaac 
aaaatcgaa aaaatcaaa tgtcagtgt gaattattc 
cgaaattag aaatatcca ttgtcctga aacgttatt 
caatatttg tagtaattg taatgatcc tgagggtct 
acaggaatt gttatcaga ttcgcaaaa aaagtcaat 
tgtgagtta ccaggagca aatttcatt gaccgaaca 
agtggaaat atcccgtaa aattgaaga cagtataaa 
taaacatga aacgaccaa aagagacgt aactgcact 
gttaacact tttggtatt agatgctca cgatccttc 
acaagacaa aagttacgt cttaaaaat ttggaaacc 
aacaagcgc tcatttaca aatgtttaa ggaatcaac 
aacttgtca catttacaa cgtttcgtt gaaaaaaat 
tggtgtttt gtccgcaac gaattggac caaattcaa 
accaactga ttattcgga agaacattt tacgaaata 
caaccgatt cctttctcc aatctctac aatcaagcc 
acaatttaa cgaatttga tacagtggg tgttgttgt 
acaaataac tgtgaatag ttttgatca aaacttgtg 
gttggcaga taattgcac caatttgtt gtttgttaa 
aactttcaa tggtccgaa aagttgtct actttttga 
taaactcaa agttggaca agttgttgc aattacgaa 
tttaatttt attcgataa gacaacaga ttttgcaca 
agctgaagc taatcattt gacggtcgt ttcatcggc 
acccagaca caagtattt gcaagaggg gcttgattt 
attgcgtca acaattacc caaagaatt ggacacttt 
tttgcaaga gtgcaacca gaaagtggc tttttgtac 
cgttaaaag tgatataac gaaaaatta caaaaattc 
gtcaaaatc tctgctaca gacgtccga tatttttga 
tgataattt ggatagttt ctactccac gattgactt 
cgataaatt gaagtgaaa gttgttaac tcgtcttag 
tccagtttg ttcacgtct ttgtctact tgtgttaag 
ttaagaata aagaaatcc ttcggtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY