General information | |||
TC003258 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 466691..470856 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC003258 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03530 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
tropharium size decreased - Number of animals showing the phenotype: 4
vitellarium size decreased - Number of animals showing the phenotype: 4
egg chamber mostly not present - Number of animals showing the phenotype: 4
Comment: only found one previtellogenic egg chamber in one ovariole |
- >TC003258 RA
MEDRPLSPI LSTQISRKW IRSKRSQKK SRVNLTNRF LDASSSNVL TPSQTENFL ESIEKDWSL DKNSDDVLK RAEILTPPQ GFNCKNEPN KVDNQGFKA SDEALKKAR TLFDDMLNP EGCNKELST GFAGFKTAS GKNSLRSEN AVKEARNRN SNEGTTTSF VGFKTASGK SSLMSKSAI KQARNKLSE DFNTKISEY NASFSGFKT ASGKCTVMS ERAINQARN KLNENFNFE DVKNAKKSD LSDEFRGFS QDMIEKNRK KLDEFRSIV TQFNKGVKK EKNLETVRE FPKNRGFQS VNLSKDRLE KARQLFQGL DDSFGRKRR FFEQEKFGF SPVRKNPSV YNSTPLKNT SFTCLESDV TPIKKIKNE TIITQNDRF VIQSCTKGN LDTWLQNLQ NERKILEVK LKVIIEKEK VLNLQKEIL ENRVQRQSG ILFTRKENS ERVLLKHGI NIDSCRVSS EINSENSPN VHLKEKLPM IKTLDGACV VPNVDNFVG LSEIEVAFN TIPGVETKL IPNGWVQNH FRWIVWKLA CYENFNFDC LTVENIMQQ LKYRYDREI DRAQRPALR KIYETDDTP QKRMVLCVA NIFNLGLNK FELELTDGW YSIRTLIDT PLCQQIANG KIQIGTKLV IFGAELLNC DGCHPLEAP DSVRLKIHC NSTRRALWH AKLGYQKCP DPCLLKLAS FYPNGGLIG CIKVYIARV YPLKYLEKQ NTHSVWRNQ AAEDLRVIE WESHIRDNL QKMEETIRK DYEMNRTKS KKSNVSVNY SEIRNIHCP ETLFNICSN CNDPEGLQE LLSDSQKKS IVSYQEQIS LTEQVEISR KIEDSINKH ETTKRDVTA LLTLLVLDA HDPSQDKSY VLKIWKPTS AHLQMFKES TTCHIYNVS LKKIGVLSA TNWTKFKPT DYSEEHFTK YNRFLSPIS TIKPQFNEF DTVGVVVQI TVNSFDQNL WLADNCTNL LFVKTFNGP KSCLLFDKL KVGQVVAIT NLILFDKTT DFAQAEANH LTVVSSAPR HKYLQEGLD LLRQQLPKE LDTFLQECN QKVAFCTVK SDITKNYKN SSKSLLQTS DIFDDNLDS FYSTIDFDK LK
- >TC003258 RA
atggaagac cggccttta tcacctatt ctaagcact caaatatca cgcaaatgg attagaagc aagagatca cagaaaaaa agtcgggtt aatttgacg aaccggttt ctggacgcg tcttccagc aatgtttta accccaagc caaaccgaa aactttttg gaaagtata gaaaaagac tggagtttg gataaaaat tctgatgat gtgttgaaa agagccgaa attttgact cctccccaa ggttttaat tgtaaaaat gaacccaac aaagttgat aatcaaggt ttcaaagcg tctgatgaa gccttgaaa aaggccaga acgttgttt gacgacatg ctaaatcct gaaggttgt aacaaggaa ttaagtact ggttttgca ggttttaaa acagcttca ggaaaaaat tccttaaga tccgaaaat gcagtcaaa gaagctagg aataggaac agtaatgag ggaactacc acaagtttt gtcggtttt aaaacagct tcagggaaa agttcccta atgtctaag agtgcaatc aaacaagct aggaataaa ttaagcgaa gattttaat acgaaaatt tcggaatat aacgcaagt ttttcgggt tttaagaca gcttcaggg aaatgtaca gtaatgtcc gaaagagca atcaaccag gccaggaat aaattaaac gagaatttt aattttgaa gacgttaaa aatgcgaaa aaatcagat ttgagtgac gagttcaga ggttttagt caagatatg attgagaaa aatcggaaa aaacttgac gaatttcgc tcgattgta acacaattt aacaaaggt gtaaaaaaa gaaaaaaac ttagaaacc gttcgggaa tttcctaaa aataggggc ttccaatca gtgaattta tcaaaagac aggctggaa aaagcgcgt caattgttc caaggtctg gacgattcg tttggtcgc aaaaggcgt ttttttgag caagaaaaa tttggtttc tctcctgtt agaaaaaat ccgagtgtt tacaactca acgcctttg aaaaacacc tcttttacg tgtctagaa tcggacgta acaccgatc aaaaaaatc aaaaacgag acaattatt acacaaaat gatagattt gtaattcag agctgcact aaaggaaat ctcgatacg tggcttcaa aatttgcaa aacgaacga aaaattctt gaagtaaaa cttaaagtt atcatcgag aaggagaaa gttttaaat ttgcaaaaa gaaattctg gaaaatagg gttcagaga cagtctgga attttattt actagaaaa gaaaatagc gaacgtgtt ttgttaaaa catggcata aacatcgat tcgtgtcgt gtttcaagt gaaattaat tcggaaaat tcgcccaat gttcatttg aaggaaaaa ctgccaatg attaaaacg ttagatggt gcttgtgtt gttccaaat gtcgataat ttcgttggt ttaagtgag attgaagta gcgtttaat acaatacca ggagtcgaa accaaatta ataccaaat ggttgggtg caaaatcat ttcagatgg attgtttgg aagttggcg tgttacgaa aattttaat ttcgattgt ttaacagtc gaaaatatt atgcaacaa ttgaaatat cgatatgat cgtgaaatt gatcgggcg caacgtcca gcacttcga aaaatttat gaaacggac gatacgccc caaaaacga atggtttta tgtgtggct aatattttt aacttgggt ttaaataaa ttcgaactg gaattgact gacggttgg tatagcatt cgaacgtta attgataca cctttatgc cagcaaatt gctaatggg aaaattcaa attggtacc aaattggtg atttttggt gcggaattg ttgaactgt gatggatgc catccatta gaggcgcct gattcagtc cgtttaaaa atccactgc aattcaacg agaagagcc ctctggcat gccaaactc ggttatcaa aagtgtcca gatccgtgc ttgctaaaa ttggcctcg ttttatcca aatgggggc ctcatcggt tgtatcaaa gtgtacata gctcgagta tatcctctg aaatatctc gaaaaacaa aatacacat tctgtttgg cgaaatcaa gcagctgaa gatttacgt gtaatagaa tgggagagt cacattcga gataatttg caaaaaatg gaagaaaca ataaggaaa gattacgaa atgaaccga acaaaatcg aaaaaatca aatgtcagt gtgaattat tccgaaatt agaaatatc cattgtcct gaaacgtta ttcaatatt tgtagtaat tgtaatgat cctgagggt ctacaggaa ttgttatca gattcgcaa aaaaagtca attgtgagt taccaggag caaatttca ttgaccgaa caagtggaa atatcccgt aaaattgaa gacagtata aataaacat gaaacgacc aaaagagac gtaactgca ctgttaaca cttttggta ttagatgct cacgatcct tcacaagac aaaagttac gtcttaaaa atttggaaa ccaacaagc gctcattta caaatgttt aaggaatca acaacttgt cacatttac aacgtttcg ttgaaaaaa attggtgtt ttgtccgca acgaattgg accaaattc aaaccaact gattattcg gaagaacat tttacgaaa tacaaccga ttcctttct ccaatctct acaatcaag ccacaattt aacgaattt gatacagtg ggtgttgtt gtacaaata actgtgaat agttttgat caaaacttg tggttggca gataattgc accaatttg ttgtttgtt aaaactttc aatggtccg aaaagttgt ctacttttt gataaactc aaagttgga caagttgtt gcaattacg aatttaatt ttattcgat aagacaaca gattttgca caagctgaa gctaatcat ttgacggtc gtttcatcg gcacccaga cacaagtat ttgcaagag gggcttgat ttattgcgt caacaatta cccaaagaa ttggacact tttttgcaa gagtgcaac cagaaagtg gctttttgt accgttaaa agtgatata acgaaaaat tacaaaaat tcgtcaaaa tctctgcta cagacgtcc gatattttt gatgataat ttggatagt ttctactcc acgattgac ttcgataaa ttgaagtga
- >TC003258 RA
ttgttgatg tgtaatggc ggtgtttac tgtttgtaa aaaataaaa atttggtgt ttgttgaaa acgttagtg aattttggg caagtttgt gaaaattcg gttttccaa cctcaaaat ggaagaccg gcctttatc acctattct aagcactca aatatcacg caaatggat tagaagcaa gagatcaca gaaaaaaag tcgggttaa tttgacgaa ccggtttct ggacgcgtc ttccagcaa tgttttaac cccaagcca aaccgaaaa ctttttgga aagtataga aaaagactg gagtttgga taaaaattc tgatgatgt gttgaaaag agccgaaat tttgactcc tccccaagg ttttaattg taaaaatga acccaacaa agttgataa tcaaggttt caaagcgtc tgatgaagc cttgaaaaa ggccagaac gttgtttga cgacatgct aaatcctga aggttgtaa caaggaatt aagtactgg ttttgcagg ttttaaaac agcttcagg aaaaaattc cttaagatc cgaaaatgc agtcaaaga agctaggaa taggaacag taatgaggg aactaccac aagttttgt cggttttaa aacagcttc agggaaaag ttccctaat gtctaagag tgcaatcaa acaagctag gaataaatt aagcgaaga ttttaatac gaaaatttc ggaatataa cgcaagttt ttcgggttt taagacagc ttcagggaa atgtacagt aatgtccga aagagcaat caaccaggc caggaataa attaaacga gaattttaa ttttgaaga cgttaaaaa tgcgaaaaa atcagattt gagtgacga gttcagagg ttttagtca agatatgat tgagaaaaa tcggaaaaa acttgacga atttcgctc gattgtaac acaatttaa caaaggtgt aaaaaaaga aaaaaactt agaaaccgt tcgggaatt tcctaaaaa taggggctt ccaatcagt gaatttatc aaaagacag gctggaaaa agcgcgtca attgttcca aggtctgga cgattcgtt tggtcgcaa aaggcgttt ttttgagca agaaaaatt tggtttctc tcctgttag aaaaaatcc gagtgttta caactcaac gcctttgaa aaacacctc ttttacgtg tctagaatc ggacgtaac accgatcaa aaaaatcaa aaacgagac aattattac acaaaatga tagatttgt aattcagag ctgcactaa aggaaatct cgatacgtg gcttcaaaa tttgcaaaa cgaacgaaa aattcttga agtaaaact taaagttat catcgagaa ggagaaagt tttaaattt gcaaaaaga aattctgga aaatagggt tcagagaca gtctggaat tttatttac tagaaaaga aaatagcga acgtgtttt gttaaaaca tggcataaa catcgattc gtgtcgtgt ttcaagtga aattaattc ggaaaattc gcccaatgt tcatttgaa ggaaaaact gccaatgat taaaacgtt agatggtgc ttgtgttgt tccaaatgt cgataattt cgttggttt aagtgagat tgaagtagc gtttaatac aataccagg agtcgaaac caaattaat accaaatgg ttgggtgca aaatcattt cagatggat tgtttggaa gttggcgtg ttacgaaaa ttttaattt cgattgttt aacagtcga aaatattat gcaacaatt gaaatatcg atatgatcg tgaaattga tcgggcgca acgtccagc acttcgaaa aatttatga aacggacga tacgcccca aaaacgaat ggttttatg tgtggctaa tatttttaa cttgggttt aaataaatt cgaactgga attgactga cggttggta tagcattcg aacgttaat tgatacacc tttatgcca gcaaattgc taatgggaa aattcaaat tggtaccaa attggtgat ttttggtgc ggaattgtt gaactgtga tggatgcca tccattaga ggcgcctga ttcagtccg tttaaaaat ccactgcaa ttcaacgag aagagccct ctggcatgc caaactcgg ttatcaaaa gtgtccaga tccgtgctt gctaaaatt ggcctcgtt ttatccaaa tgggggcct catcggttg tatcaaagt gtacatagc tcgagtata tcctctgaa atatctcga aaaacaaaa tacacattc tgtttggcg aaatcaagc agctgaaga tttacgtgt aatagaatg ggagagtca cattcgaga taatttgca aaaaatgga agaaacaat aaggaaaga ttacgaaat gaaccgaac aaaatcgaa aaaatcaaa tgtcagtgt gaattattc cgaaattag aaatatcca ttgtcctga aacgttatt caatatttg tagtaattg taatgatcc tgagggtct acaggaatt gttatcaga ttcgcaaaa aaagtcaat tgtgagtta ccaggagca aatttcatt gaccgaaca agtggaaat atcccgtaa aattgaaga cagtataaa taaacatga aacgaccaa aagagacgt aactgcact gttaacact tttggtatt agatgctca cgatccttc acaagacaa aagttacgt cttaaaaat ttggaaacc aacaagcgc tcatttaca aatgtttaa ggaatcaac aacttgtca catttacaa cgtttcgtt gaaaaaaat tggtgtttt gtccgcaac gaattggac caaattcaa accaactga ttattcgga agaacattt tacgaaata caaccgatt cctttctcc aatctctac aatcaagcc acaatttaa cgaatttga tacagtggg tgttgttgt acaaataac tgtgaatag ttttgatca aaacttgtg gttggcaga taattgcac caatttgtt gtttgttaa aactttcaa tggtccgaa aagttgtct actttttga taaactcaa agttggaca agttgttgc aattacgaa tttaatttt attcgataa gacaacaga ttttgcaca agctgaagc taatcattt gacggtcgt ttcatcggc acccagaca caagtattt gcaagaggg gcttgattt attgcgtca acaattacc caaagaatt ggacacttt tttgcaaga gtgcaacca gaaagtggc tttttgtac cgttaaaag tgatataac gaaaaatta caaaaattc gtcaaaatc tctgctaca gacgtccga tatttttga tgataattt ggatagttt ctactccac gattgactt cgataaatt gaagtgaaa gttgttaac tcgtcttag tccagtttg ttcacgtct ttgtctact tgtgttaag ttaagaata aagaaatcc ttcggtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
