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Gene information
General information
TC003258 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 466691..470856
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003258
      iBeetle screen iB_03530

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
      Days post injection


      Fertility
      egg production: number no/almost no eggs -
      tropharium size decreased - Number of animals showing the phenotype: 4

      vitellarium size decreased - Number of animals showing the phenotype: 4
      egg chamber mostly not present - Number of animals showing the phenotype: 4
      Comment: only found one previtellogenic egg chamber in one ovariole
      ovary size decreased - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003258 RA
        MEDRPLSPI LSTQISRKW IRSKRSQKK SRVNLTNRF 
        LDASSSNVL TPSQTENFL ESIEKDWSL DKNSDDVLK 
        RAEILTPPQ GFNCKNEPN KVDNQGFKA SDEALKKAR 
        TLFDDMLNP EGCNKELST GFAGFKTAS GKNSLRSEN 
        AVKEARNRN SNEGTTTSF VGFKTASGK SSLMSKSAI 
        KQARNKLSE DFNTKISEY NASFSGFKT ASGKCTVMS 
        ERAINQARN KLNENFNFE DVKNAKKSD LSDEFRGFS 
        QDMIEKNRK KLDEFRSIV TQFNKGVKK EKNLETVRE 
        FPKNRGFQS VNLSKDRLE KARQLFQGL DDSFGRKRR 
        FFEQEKFGF SPVRKNPSV YNSTPLKNT SFTCLESDV 
        TPIKKIKNE TIITQNDRF VIQSCTKGN LDTWLQNLQ 
        NERKILEVK LKVIIEKEK VLNLQKEIL ENRVQRQSG 
        ILFTRKENS ERVLLKHGI NIDSCRVSS EINSENSPN 
        VHLKEKLPM IKTLDGACV VPNVDNFVG LSEIEVAFN 
        TIPGVETKL IPNGWVQNH FRWIVWKLA CYENFNFDC 
        LTVENIMQQ LKYRYDREI DRAQRPALR KIYETDDTP 
        QKRMVLCVA NIFNLGLNK FELELTDGW YSIRTLIDT 
        PLCQQIANG KIQIGTKLV IFGAELLNC DGCHPLEAP 
        DSVRLKIHC NSTRRALWH AKLGYQKCP DPCLLKLAS 
        FYPNGGLIG CIKVYIARV YPLKYLEKQ NTHSVWRNQ 
        AAEDLRVIE WESHIRDNL QKMEETIRK DYEMNRTKS 
        KKSNVSVNY SEIRNIHCP ETLFNICSN CNDPEGLQE 
        LLSDSQKKS IVSYQEQIS LTEQVEISR KIEDSINKH 
        ETTKRDVTA LLTLLVLDA HDPSQDKSY VLKIWKPTS 
        AHLQMFKES TTCHIYNVS LKKIGVLSA TNWTKFKPT 
        DYSEEHFTK YNRFLSPIS TIKPQFNEF DTVGVVVQI 
        TVNSFDQNL WLADNCTNL LFVKTFNGP KSCLLFDKL 
        KVGQVVAIT NLILFDKTT DFAQAEANH LTVVSSAPR 
        HKYLQEGLD LLRQQLPKE LDTFLQECN QKVAFCTVK 
        SDITKNYKN SSKSLLQTS DIFDDNLDS FYSTIDFDK 
        LK
      CDS sequence
      • >TC003258 RA
        atggaagac cggccttta tcacctatt ctaagcact 
        caaatatca cgcaaatgg attagaagc aagagatca 
        cagaaaaaa agtcgggtt aatttgacg aaccggttt 
        ctggacgcg tcttccagc aatgtttta accccaagc 
        caaaccgaa aactttttg gaaagtata gaaaaagac 
        tggagtttg gataaaaat tctgatgat gtgttgaaa 
        agagccgaa attttgact cctccccaa ggttttaat 
        tgtaaaaat gaacccaac aaagttgat aatcaaggt 
        ttcaaagcg tctgatgaa gccttgaaa aaggccaga 
        acgttgttt gacgacatg ctaaatcct gaaggttgt 
        aacaaggaa ttaagtact ggttttgca ggttttaaa 
        acagcttca ggaaaaaat tccttaaga tccgaaaat 
        gcagtcaaa gaagctagg aataggaac agtaatgag 
        ggaactacc acaagtttt gtcggtttt aaaacagct 
        tcagggaaa agttcccta atgtctaag agtgcaatc 
        aaacaagct aggaataaa ttaagcgaa gattttaat 
        acgaaaatt tcggaatat aacgcaagt ttttcgggt 
        tttaagaca gcttcaggg aaatgtaca gtaatgtcc 
        gaaagagca atcaaccag gccaggaat aaattaaac 
        gagaatttt aattttgaa gacgttaaa aatgcgaaa 
        aaatcagat ttgagtgac gagttcaga ggttttagt 
        caagatatg attgagaaa aatcggaaa aaacttgac 
        gaatttcgc tcgattgta acacaattt aacaaaggt 
        gtaaaaaaa gaaaaaaac ttagaaacc gttcgggaa 
        tttcctaaa aataggggc ttccaatca gtgaattta 
        tcaaaagac aggctggaa aaagcgcgt caattgttc 
        caaggtctg gacgattcg tttggtcgc aaaaggcgt 
        ttttttgag caagaaaaa tttggtttc tctcctgtt 
        agaaaaaat ccgagtgtt tacaactca acgcctttg 
        aaaaacacc tcttttacg tgtctagaa tcggacgta 
        acaccgatc aaaaaaatc aaaaacgag acaattatt 
        acacaaaat gatagattt gtaattcag agctgcact 
        aaaggaaat ctcgatacg tggcttcaa aatttgcaa 
        aacgaacga aaaattctt gaagtaaaa cttaaagtt 
        atcatcgag aaggagaaa gttttaaat ttgcaaaaa 
        gaaattctg gaaaatagg gttcagaga cagtctgga 
        attttattt actagaaaa gaaaatagc gaacgtgtt 
        ttgttaaaa catggcata aacatcgat tcgtgtcgt 
        gtttcaagt gaaattaat tcggaaaat tcgcccaat 
        gttcatttg aaggaaaaa ctgccaatg attaaaacg 
        ttagatggt gcttgtgtt gttccaaat gtcgataat 
        ttcgttggt ttaagtgag attgaagta gcgtttaat 
        acaatacca ggagtcgaa accaaatta ataccaaat 
        ggttgggtg caaaatcat ttcagatgg attgtttgg 
        aagttggcg tgttacgaa aattttaat ttcgattgt 
        ttaacagtc gaaaatatt atgcaacaa ttgaaatat 
        cgatatgat cgtgaaatt gatcgggcg caacgtcca 
        gcacttcga aaaatttat gaaacggac gatacgccc 
        caaaaacga atggtttta tgtgtggct aatattttt 
        aacttgggt ttaaataaa ttcgaactg gaattgact 
        gacggttgg tatagcatt cgaacgtta attgataca 
        cctttatgc cagcaaatt gctaatggg aaaattcaa 
        attggtacc aaattggtg atttttggt gcggaattg 
        ttgaactgt gatggatgc catccatta gaggcgcct 
        gattcagtc cgtttaaaa atccactgc aattcaacg 
        agaagagcc ctctggcat gccaaactc ggttatcaa 
        aagtgtcca gatccgtgc ttgctaaaa ttggcctcg 
        ttttatcca aatgggggc ctcatcggt tgtatcaaa 
        gtgtacata gctcgagta tatcctctg aaatatctc 
        gaaaaacaa aatacacat tctgtttgg cgaaatcaa 
        gcagctgaa gatttacgt gtaatagaa tgggagagt 
        cacattcga gataatttg caaaaaatg gaagaaaca 
        ataaggaaa gattacgaa atgaaccga acaaaatcg 
        aaaaaatca aatgtcagt gtgaattat tccgaaatt 
        agaaatatc cattgtcct gaaacgtta ttcaatatt 
        tgtagtaat tgtaatgat cctgagggt ctacaggaa 
        ttgttatca gattcgcaa aaaaagtca attgtgagt 
        taccaggag caaatttca ttgaccgaa caagtggaa 
        atatcccgt aaaattgaa gacagtata aataaacat 
        gaaacgacc aaaagagac gtaactgca ctgttaaca 
        cttttggta ttagatgct cacgatcct tcacaagac 
        aaaagttac gtcttaaaa atttggaaa ccaacaagc 
        gctcattta caaatgttt aaggaatca acaacttgt 
        cacatttac aacgtttcg ttgaaaaaa attggtgtt 
        ttgtccgca acgaattgg accaaattc aaaccaact 
        gattattcg gaagaacat tttacgaaa tacaaccga 
        ttcctttct ccaatctct acaatcaag ccacaattt 
        aacgaattt gatacagtg ggtgttgtt gtacaaata 
        actgtgaat agttttgat caaaacttg tggttggca 
        gataattgc accaatttg ttgtttgtt aaaactttc 
        aatggtccg aaaagttgt ctacttttt gataaactc 
        aaagttgga caagttgtt gcaattacg aatttaatt 
        ttattcgat aagacaaca gattttgca caagctgaa 
        gctaatcat ttgacggtc gtttcatcg gcacccaga 
        cacaagtat ttgcaagag gggcttgat ttattgcgt 
        caacaatta cccaaagaa ttggacact tttttgcaa 
        gagtgcaac cagaaagtg gctttttgt accgttaaa 
        agtgatata acgaaaaat tacaaaaat tcgtcaaaa 
        tctctgcta cagacgtcc gatattttt gatgataat 
        ttggatagt ttctactcc acgattgac ttcgataaa 
        ttgaagtga
      mRNA sequence
      • >TC003258 RA
        ttgttgatg tgtaatggc ggtgtttac tgtttgtaa 
        aaaataaaa atttggtgt ttgttgaaa acgttagtg 
        aattttggg caagtttgt gaaaattcg gttttccaa 
        cctcaaaat ggaagaccg gcctttatc acctattct 
        aagcactca aatatcacg caaatggat tagaagcaa 
        gagatcaca gaaaaaaag tcgggttaa tttgacgaa 
        ccggtttct ggacgcgtc ttccagcaa tgttttaac 
        cccaagcca aaccgaaaa ctttttgga aagtataga 
        aaaagactg gagtttgga taaaaattc tgatgatgt 
        gttgaaaag agccgaaat tttgactcc tccccaagg 
        ttttaattg taaaaatga acccaacaa agttgataa 
        tcaaggttt caaagcgtc tgatgaagc cttgaaaaa 
        ggccagaac gttgtttga cgacatgct aaatcctga 
        aggttgtaa caaggaatt aagtactgg ttttgcagg 
        ttttaaaac agcttcagg aaaaaattc cttaagatc 
        cgaaaatgc agtcaaaga agctaggaa taggaacag 
        taatgaggg aactaccac aagttttgt cggttttaa 
        aacagcttc agggaaaag ttccctaat gtctaagag 
        tgcaatcaa acaagctag gaataaatt aagcgaaga 
        ttttaatac gaaaatttc ggaatataa cgcaagttt 
        ttcgggttt taagacagc ttcagggaa atgtacagt 
        aatgtccga aagagcaat caaccaggc caggaataa 
        attaaacga gaattttaa ttttgaaga cgttaaaaa 
        tgcgaaaaa atcagattt gagtgacga gttcagagg 
        ttttagtca agatatgat tgagaaaaa tcggaaaaa 
        acttgacga atttcgctc gattgtaac acaatttaa 
        caaaggtgt aaaaaaaga aaaaaactt agaaaccgt 
        tcgggaatt tcctaaaaa taggggctt ccaatcagt 
        gaatttatc aaaagacag gctggaaaa agcgcgtca 
        attgttcca aggtctgga cgattcgtt tggtcgcaa 
        aaggcgttt ttttgagca agaaaaatt tggtttctc 
        tcctgttag aaaaaatcc gagtgttta caactcaac 
        gcctttgaa aaacacctc ttttacgtg tctagaatc 
        ggacgtaac accgatcaa aaaaatcaa aaacgagac 
        aattattac acaaaatga tagatttgt aattcagag 
        ctgcactaa aggaaatct cgatacgtg gcttcaaaa 
        tttgcaaaa cgaacgaaa aattcttga agtaaaact 
        taaagttat catcgagaa ggagaaagt tttaaattt 
        gcaaaaaga aattctgga aaatagggt tcagagaca 
        gtctggaat tttatttac tagaaaaga aaatagcga 
        acgtgtttt gttaaaaca tggcataaa catcgattc 
        gtgtcgtgt ttcaagtga aattaattc ggaaaattc 
        gcccaatgt tcatttgaa ggaaaaact gccaatgat 
        taaaacgtt agatggtgc ttgtgttgt tccaaatgt 
        cgataattt cgttggttt aagtgagat tgaagtagc 
        gtttaatac aataccagg agtcgaaac caaattaat 
        accaaatgg ttgggtgca aaatcattt cagatggat 
        tgtttggaa gttggcgtg ttacgaaaa ttttaattt 
        cgattgttt aacagtcga aaatattat gcaacaatt 
        gaaatatcg atatgatcg tgaaattga tcgggcgca 
        acgtccagc acttcgaaa aatttatga aacggacga 
        tacgcccca aaaacgaat ggttttatg tgtggctaa 
        tatttttaa cttgggttt aaataaatt cgaactgga 
        attgactga cggttggta tagcattcg aacgttaat 
        tgatacacc tttatgcca gcaaattgc taatgggaa 
        aattcaaat tggtaccaa attggtgat ttttggtgc 
        ggaattgtt gaactgtga tggatgcca tccattaga 
        ggcgcctga ttcagtccg tttaaaaat ccactgcaa 
        ttcaacgag aagagccct ctggcatgc caaactcgg 
        ttatcaaaa gtgtccaga tccgtgctt gctaaaatt 
        ggcctcgtt ttatccaaa tgggggcct catcggttg 
        tatcaaagt gtacatagc tcgagtata tcctctgaa 
        atatctcga aaaacaaaa tacacattc tgtttggcg 
        aaatcaagc agctgaaga tttacgtgt aatagaatg 
        ggagagtca cattcgaga taatttgca aaaaatgga 
        agaaacaat aaggaaaga ttacgaaat gaaccgaac 
        aaaatcgaa aaaatcaaa tgtcagtgt gaattattc 
        cgaaattag aaatatcca ttgtcctga aacgttatt 
        caatatttg tagtaattg taatgatcc tgagggtct 
        acaggaatt gttatcaga ttcgcaaaa aaagtcaat 
        tgtgagtta ccaggagca aatttcatt gaccgaaca 
        agtggaaat atcccgtaa aattgaaga cagtataaa 
        taaacatga aacgaccaa aagagacgt aactgcact 
        gttaacact tttggtatt agatgctca cgatccttc 
        acaagacaa aagttacgt cttaaaaat ttggaaacc 
        aacaagcgc tcatttaca aatgtttaa ggaatcaac 
        aacttgtca catttacaa cgtttcgtt gaaaaaaat 
        tggtgtttt gtccgcaac gaattggac caaattcaa 
        accaactga ttattcgga agaacattt tacgaaata 
        caaccgatt cctttctcc aatctctac aatcaagcc 
        acaatttaa cgaatttga tacagtggg tgttgttgt 
        acaaataac tgtgaatag ttttgatca aaacttgtg 
        gttggcaga taattgcac caatttgtt gtttgttaa 
        aactttcaa tggtccgaa aagttgtct actttttga 
        taaactcaa agttggaca agttgttgc aattacgaa 
        tttaatttt attcgataa gacaacaga ttttgcaca 
        agctgaagc taatcattt gacggtcgt ttcatcggc 
        acccagaca caagtattt gcaagaggg gcttgattt 
        attgcgtca acaattacc caaagaatt ggacacttt 
        tttgcaaga gtgcaacca gaaagtggc tttttgtac 
        cgttaaaag tgatataac gaaaaatta caaaaattc 
        gtcaaaatc tctgctaca gacgtccga tatttttga 
        tgataattt ggatagttt ctactccac gattgactt 
        cgataaatt gaagtgaaa gttgttaac tcgtcttag 
        tccagtttg ttcacgtct ttgtctact tgtgttaag 
        ttaagaata aagaaatcc ttcggtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY