Status
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Gene information
General information
TC003243 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 243179..246695
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003243
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03527

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003243 RA
      MKTSETGAV VSLIFTLCS AILTGNYEH ITDNPVFGC 
      RWIGGISPG VVCLCRDDA EEMYITKKS ISSYDTSSI 
      EIHCKSVRF GPESITEMR NLRNIKLKS IQSLNFDEH 
      SLKWYGYRD IDHHDEPYD ISTPSLQIE IENSTIGTI 
      SSYTFEGRI NKIRFDGVT VQNLSPFAF NSLRQTQEI 
      VLRNVELLN AREQAFKKF STDNFELNG VKSTIITSR 
      FVFNVTVYN SFKVTNCVF DTVRPGGFI VFNPRNFQV 
      SDTTINHLD AEAFKVTTR GDVLFRSNK FNVINDGAF 
      LGIDLNPDE TAIEATMSF DSNVFTKLT RDSLTVNEN 
      FRPKFANLF INETCDCTS IDHNIKDTE FYSEIKCWH 
      EREFVTVKE FKTNKCSII QSYSTVIVI VVVVFTLCV 
      IIGTVLVVY YRRVYLSKK YGSEKGTKN GNLSMIVPD 
      GRTYRETEL HVIVERADL LTTDL
    CDS sequence
    • >TC003243 RA
      atgaagaca tctgaaacg ggagcagtc gtttcgtta 
      atatttact ttatgttcc gcaatttta accggaaat 
      tatgaacac atcaccgat aatccggtg tttggatgt 
      agatggata ggtggaatt agtccagga gttgtctgc 
      ttgtgtcgg gatgatgcc gaggaaatg tacatcacg 
      aaaaaatcc atctcgagt tacgacacc tccagcatt 
      gagatccac tgtaaatcg gttcgtttc ggcccggaa 
      tcaattaca gaaatgcgt aatcttcgc aacatcaaa 
      ttaaaatca atccaatca ctaaatttc gacgaacac 
      agcttaaaa tggtacggc tatcgagac atagaccat 
      catgacgaa ccctacgac atcagcaca ccttccctt 
      caaatcgaa atcgaaaat tcaaccatt ggaacaatt 
      tcttcgtac accttcgaa ggccgcatt aataaaatc 
      cgattcgat ggagtcacc gttcagaat ttatcaccg 
      ttcgctttt aattccctg agacagaca caggagatc 
      gttttgcga aacgtcgaa ttgctaaat gcacgggaa 
      caagccttc aagaaattt tcaacggat aatttcgaa 
      ctgaacggc gttaaatcc acgattatt acaagccgg 
      ttcgtgttc aacgtcaca gtttacaac agttttaaa 
      gtcacgaat tgtgtgttt gacactgta cgcccaggg 
      ggtttcatc gttttcaac cctcgtaat ttccaagtt 
      agcgacact acaatcaac catttggac gccgaagcg 
      tttaaagtg accacacga ggggatgtt ctgttccgt 
      agcaataaa ttcaacgta attaacgac ggtgcgttc 
      cttggaatc gatctcaat ccggatgaa acggcgatt 
      gaggcgaca atgtcgttc gattccaac gtttttacc 
      aaattaacg cgagattct ttgactgtt aacgaaaat 
      ttccggccg aaatttgcc aatttgttc atcaacgag 
      acgtgtgat tgcacaagc atcgaccac aatattaaa 
      gacactgaa ttctactcg gaaattaag tgttggcac 
      gagagggaa tttgttact gtcaaggaa ttcaaaacc 
      aacaagtgt tcgattatt cagagttac tccactgtt 
      attgttatt gtcgttgtg gtttttact ctctgtgtt 
      atcatcggg actgttctt gtggtgtat tatcgtaga 
      gtgtatttg agcaagaaa tacggtagt gagaaaggt 
      accaaaaat gggaattta agtatgatt gttccggat 
      ggaaggact tatcgtgaa acggaactt catgtgata 
      gtggaacga gctgatctc ttgaccacc gatttatag
    mRNA sequence
    • >TC003243 RA
      gatagtcga taacaataa atgttcacg tcggtgtga 
      gattttgtc cataatcag tagtgacgt gttgtgata 
      acattgttt cccgaaaaa acaagtttt ctgaaacgc 
      aataactgt gaaaattac cttttccaa cacaaacta 
      gtctttgtg ggtttcgca acgagaaaa atgatcctt 
      ccaaggaat aaaaaatga agacatctg aaacgggag 
      cagtcgttt cgttaatat ttactttat gttccgcaa 
      ttttaaccg gaaattatg aacacatca ccgataatc 
      cggtgtttg gatgtagat ggataggtg gaattagtc 
      caggagttg tctgcttgt gtcgggatg atgccgagg 
      aaatgtaca tcacgaaaa aatccatct cgagttacg 
      acacctcca gcattgaga tccactgta aatcggttc 
      gtttcggcc cggaatcaa ttacagaaa tgcgtaatc 
      ttcgcaaca tcaaattaa aatcaatcc aatcactaa 
      atttcgacg aacacagct taaaatggt acggctatc 
      gagacatag accatcatg acgaaccct acgacatca 
      gcacacctt cccttcaaa tcgaaatcg aaaattcaa 
      ccattggaa caatttctt cgtacacct tcgaaggcc 
      gcattaata aaatccgat tcgatggag tcaccgttc 
      agaatttat caccgttcg cttttaatt ccctgagac 
      agacacagg agatcgttt tgcgaaacg tcgaattgc 
      taaatgcac gggaacaag ccttcaaga aattttcaa 
      cggataatt tcgaactga acggcgtta aatccacga 
      ttattacaa gccggttcg tgttcaacg tcacagttt 
      acaacagtt ttaaagtca cgaattgtg tgtttgaca 
      ctgtacgcc cagggggtt tcatcgttt tcaaccctc 
      gtaatttcc aagttagcg acactacaa tcaaccatt 
      tggacgccg aagcgttta aagtgacca cacgagggg 
      atgttctgt tccgtagca ataaattca acgtaatta 
      acgacggtg cgttccttg gaatcgatc tcaatccgg 
      atgaaacgg cgattgagg cgacaatgt cgttcgatt 
      ccaacgttt ttaccaaat taacgcgag attctttga 
      ctgttaacg aaaatttcc ggccgaaat ttgccaatt 
      tgttcatca acgagacgt gtgattgca caagcatcg 
      accacaata ttaaagaca ctgaattct actcggaaa 
      ttaagtgtt ggcacgaga gggaatttg ttactgtca 
      aggaattca aaaccaaca agtgttcga ttattcaga 
      gttactcca ctgttattg ttattgtcg ttgtggttt 
      ttactctct gtgttatca tcgggactg ttcttgtgg 
      tgtattatc gtagagtgt atttgagca agaaatacg 
      gtagtgaga aaggtacca aaaatggga atttaagta 
      tgattgttc cggatggaa ggacttatc gtgaaacgg 
      aacttcatg tgatagtgg aacgagctg atctcttga 
      ccaccgatt tatagtcat tgcacagta ttgttggac 
      tgatactag aactaggtt ttaatttat tttggtacg 
      aattgcttc ataaacgtg tacatgtac ataaaaatt 
      gtataaata attgactca gttaccgaa aataaagta 
      gtaaacgaa cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY