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Gene information
General information
TC003242 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 231807..240523
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003242
    iBeetle screen iB_03526

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    gut partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: head and thorax are either partial missing or appear partial fused  
    prothorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: head and thorax are either partial missing or appear partial fused  
    abdomen segment partiallyfused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003242 RA
      MGVRSWRSF FVLLWVYFF GIKLGRGDT ICDLKGCNC 
      TVKVAKWKS VNCSLSGVQ ELRLDLYRI PLETVDVFI 
      TGGEKIALG PKSFDQMPG LALLHIEGT RSVVMEKQS 
      FRNVTTPSL IIQIQNCDQ LSIKTGAFD GVQSSIEVE 
      IVRCGSVNV EKTAFGKLK SALFRDVEE LVLATETFE 
      FKNQGSIGR HGPVTTIFF DHVTITTIP PRTFFSSLA 
      DVTFRNSHI TEIKSEAFS ANQISSITF TNSSIDYID 
      SNSFISRTL IYNFKLQKC TIKQIREGA VMSAITNLT 
      VQHSNITEI QSGAINSTV AKVDISDNA IRNLQSSSV 
      TFNNWNKIS FDHNIVFNL HAHSIQAPV NPDVVEFSF 
      SGNEIFKFS QSALSFVPD LDDHVFVFK DNFFEQTCH 
      CTLDEWIVE LINANITTK INKIANNTF CRVTDFLSQ 
      CFELKVGVI NVKNFTQLV CNSNFTVVC EPYKGETKI 
      VNTTIAIFT DQDSDSSSH WLTVIITTT SILILLVII 
      TSTVMLIRG SRWLKRKGY FRKIQYNQN NEHSNDEEN 
      TIETVDEGD KLDISEDLT MELLQDLSK KLDDPLTHQ 
      EASEMIEQL YQKYIHNMG SENNNQQED EAHLYEELG 
      NLQIADANK QQSGPRSIL RLMEEKFNT HFEDIDADD 
      RPALVGEYS EPSDAAVHL YSELRQNKD ESENKDSLK 
      SRGSSNMAF RPLPDKPGS SKML
    CDS sequence
    • >TC003242 RA
      atgggtgtc cgatcgtgg cggtccttc tttgtgctt 
      ctgtgggtg tattttttt gggataaag ctcgggaga 
      ggagacacg atatgtgac ctaaagggc tgcaattgc 
      accgtaaag gtggccaag tggaagagt gttaattgc 
      tccctgagt ggagttcag gaactgaga ctggacttg 
      taccggata cctctcgaa accgtcgat gtgttcatc 
      acaggcggt gaaaaaatc gccttgggt ccaaaatcg 
      ttcgaccag atgcccggt ctggccctt cttcacata 
      gaaggcact cgttcagtt gtgatggaa aaacagtcc 
      tttcgcaac gtaaccact cctagtcta ataattcaa 
      atccaaaac tgtgatcaa ttaagcata aagactggt 
      gcattcgac ggtgtacag agttccata gaagttgaa 
      attgtacga tgtggttca gtgaatgtg gaaaaaaca 
      gcgttcggg aagctaaaa agtgctctc tttagagac 
      gttgaggaa ttggtctta gctacggag acttttgag 
      tttaaaaat cagggcagt attggacgg catggtccc 
      gtcaccact attttcttc gaccatgtg acaataaca 
      acaatcccg ccccgcacg tttttctca agtttagcc 
      gacgtaacc tttcgtaat tcccacatt accgaaatc 
      aaatcggaa gcattctca gcgaaccaa atttcctcg 
      ataacattt acgaattca tcgatcgat tacatcgac 
      agcaactca ttcataagt cggacccta atttacaac 
      ttcaaactg cagaaatgc acgattaaa caaatccga 
      gaaggggct gtcatgtcc gccatcacc aacctaact 
      gttcaacat tccaatata acagaaatc cagagcgga 
      gccatcaac agcaccgtc gccaaagtg gacatctcc 
      gacaacgca atccgaaac ctccagtcc tcgagtgtg 
      actttcaac aattggaac aaaatctcg tttgatcac 
      aacatcgtt ttcaattta cacgcgcac tcgatccaa 
      gcgcctgtt aatccagac gttgttgaa ttctcattc 
      agcgggaac gaaatcttc aaattctcc caaagtgcc 
      ttaagtttc gtgccagat ttagacgac catgttttc 
      gtattcaaa gacaatttc ttcgaacaa acgtgccat 
      tgtacttta gacgaatgg atcgtcgaa ctcatcaat 
      gccaacatt acgaccaaa atcaacaaa atcgccaac 
      aacaccttc tgccgagtc accgatttt ttatcgcaa 
      tgtttcgaa ctaaaagtc ggtgttata aacgtgaaa 
      aacttcact caactcgtt tgcaactca aactttacc 
      gttgtctgc gaaccttac aaaggcgaa acgaaaatt 
      gtcaatacc actattgcg attttcacc gatcaagac 
      tcggattcg tcaagccat tggttgact gttatcata 
      actacgact tcaatccta attctgctc gtaattatc 
      acttccacc gttatgttg attagggga agtcgctgg 
      ttgaaacgt aaaggttac ttccgtaaa atccaatac 
      aaccaaaac aacgaacac agcaatgat gaagaaaac 
      accattgaa acggtcgat gaaggcgac aagcttgac 
      atttctgaa gacttgacc atggagtta ctccaagac 
      ttgtcaaag aaactcgat gacccatta acccatcaa 
      gaagcatcg gaaatgatc gaacaactt taccaaaaa 
      tacatacat aacatgggc tcggaaaac aacaaccaa 
      caagaagac gaggcccat ttgtacgaa gagctgggg 
      aatttgcaa attgctgat gcaaataaa caacaaagt 
      ggccccaga agcatttta cgtttgatg gaagagaag 
      tttaacaca cattttgag gatatagat gccgatgat 
      cgtcctgcc cttgttggg gaatattca gaaccgtcg 
      gatgccgca gttcatttg tattccgaa ctgaggcaa 
      aataaggac gaaagtgag aataaagac tcgctaaag 
      tcgcgaggt tcgagcaat atggccttc aggccattg 
      cctgataaa ccaggatcg tcgaaaatg ttgtag
    mRNA sequence
    • >TC003242 RA
      actcccaag agagacaac agcgcccca aacaaagac 
      accacatga aacaccagc caagagcaa agcaaagca 
      attcaaaag cgccagtcg ttcagaaca gtcccaagt 
      gatagtttc cctatcgca aaaaagcac caaaacaga 
      ctcatgcag gtcaaatca tgtcgtttg cagtgtttg 
      tgccaaacg taaccgatg tacaaccag tgcaagaat 
      gagtgtacg aaatgggtg tccgatcgt ggcggtcct 
      tctttgtgc ttctgtggg tgtattttt ttgggataa 
      agctcggga gaggagaca cgatatgtg acctaaagg 
      gctgcaatt gcaccgtaa aggtggcca agtggaaga 
      gtgttaatt gctccctga gtggagttc aggaactga 
      gactggact tgtaccgga tacctctcg aaaccgtcg 
      atgtgttca tcacaggcg gtgaaaaaa tcgccttgg 
      gtccaaaat cgttcgacc agatgcccg gtctggccc 
      ttcttcaca tagaaggca ctcgttcag ttgtgatgg 
      aaaaacagt cctttcgca acgtaacca ctcctagtc 
      taataattc aaatccaaa actgtgatc aattaagca 
      taaagactg gtgcattcg acggtgtac agagttcca 
      tagaagttg aaattgtac gatgtggtt cagtgaatg 
      tggaaaaaa cagcgttcg ggaagctaa aaagtgctc 
      tctttagag acgttgagg aattggtct tagctacgg 
      agacttttg agtttaaaa atcagggca gtattggac 
      ggcatggtc ccgtcacca ctattttct tcgaccatg 
      tgacaataa caacaatcc cgccccgca cgtttttct 
      caagtttag ccgacgtaa cctttcgta attcccaca 
      ttaccgaaa tcaaatcgg aagcattct cagcgaacc 
      aaatttcct cgataacat ttacgaatt catcgatcg 
      attacatcg acagcaact cattcataa gtcggaccc 
      taatttaca acttcaaac tgcagaaat gcacgatta 
      aacaaatcc gagaagggg ctgtcatgt ccgccatca 
      ccaacctaa ctgttcaac attccaata taacagaaa 
      tccagagcg gagccatca acagcaccg tcgccaaag 
      tggacatct ccgacaacg caatccgaa acctccagt 
      cctcgagtg tgactttca acaattgga acaaaatct 
      cgtttgatc acaacatcg ttttcaatt tacacgcgc 
      actcgatcc aagcgcctg ttaatccag acgttgttg 
      aattctcat tcagcggga acgaaatct tcaaattct 
      cccaaagtg ccttaagtt tcgtgccag atttagacg 
      accatgttt tcgtattca aagacaatt tcttcgaac 
      aaacgtgcc attgtactt tagacgaat ggatcgtcg 
      aactcatca atgccaaca ttacgacca aaatcaaca 
      aaatcgcca acaacacct tctgccgag tcaccgatt 
      ttttatcgc aatgtttcg aactaaaag tcggtgtta 
      taaacgtga aaaacttca ctcaactcg tttgcaact 
      caaacttta ccgttgtct gcgaacctt acaaaggcg 
      aaacgaaaa ttgtcaata ccactattg cgattttca 
      ccgatcaag actcggatt cgtcaagcc attggttga 
      ctgttatca taactacga cttcaatcc taattctgc 
      tcgtaatta tcacttcca ccgttatgt tgattaggg 
      gaagtcgct ggttgaaac gtaaaggtt acttccgta 
      aaatccaat acaaccaaa acaacgaac acagcaatg 
      atgaagaaa acaccattg aaacggtcg atgaaggcg 
      acaagcttg acatttctg aagacttga ccatggagt 
      tactccaag acttgtcaa agaaactcg atgacccat 
      taacccatc aagaagcat cggaaatga tcgaacaac 
      tttaccaaa aatacatac ataacatgg gctcggaaa 
      acaacaacc aacaagaag acgaggccc atttgtacg 
      aagagctgg ggaatttgc aaattgctg atgcaaata 
      aacaacaaa gtggcccca gaagcattt tacgtttga 
      tggaagaga agtttaaca cacattttg aggatatag 
      atgccgatg atcgtcctg cccttgttg gggaatatt 
      cagaaccgt cggatgccg cagttcatt tgtattccg 
      aactgaggc aaaataagg acgaaagtg agaataaag 
      actcgctaa agtcgcgag gttcgagca atatggcct 
      tcaggccat tgcctgata aaccaggat cgtcgaaaa 
      tgttgtagt gatctcgtg ttattctat tccagacaa 
      tcagtgcct cgtgaaatg agttgtagc tgtgataat 
      gtacagtgg tggcaacgc aatttgtgt tgtttacgt 
      gtatatttt gtaatttta ttttgattc aatagtatt 
      ttatattaa tgattactt gcgttgtat agtttatgt 
      tatcggcat cattttatc aagttactt atgttcttt 
      gtgcgatgt gagaggatg tgtaatgaa attattatg 
      aaatatact ttccgtaaa act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY