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Gene information
General information
TC003240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 199797..206593
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003240
    iBeetle screen iB_03525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (22 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003240 RA
      MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK 
CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR 
CLDRSLQVP HNNPLLCHR QNPLNSEFV MECCKNEDY 
CNVHLSPRL MQKPQAPKF SNFTTAEIV LMSVIPTML 
LCFIGVGVY YFYKTKHKR GGLHHHLRH GEDSIEAPD 
HPILKGVSL QHMIEMTTS GSGSGLPLL VQRSIARQI 
QLVEIIGQG RFGEVWRGR WRGENVAVK IFSSREERS 
WFREAEIYQ TVMLRHDNI LGFIAADNK DNGTWTQLW 
LITDYHEYG SLFDFLGRN TLDTTGMIR MALSIATGL 
AHLHMDIMG TQGKPAIAH RDLKSKNIL VKSNFTCAI 
GDLGLAVRH DATTDTVDI PLNNRVGTK RYMAPEVLD 
ETMNVNHFD SFKRADVYA LGLIFWEIA RRCNVGGIY 
DEYQLPFYD AVPSDPTIE EMRRVVCVD KQRPNIPNR 
WQSCEALRV MLKLMKECW YHNATARLT ALRIKKTLS 
NFRTSEELK M
    • >TC003240 RB
      MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK 
CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR 
CLDKRQFFP PEEPRWCHS RPTTEYVQR CLDRSLQVP 
HNNPLLCHR QNPLNSEFV MECCKNEDY CNVHLSPRL 
MQKPQAPKF SNFTTAEIV LMSVIPTML LCFIGVGVY 
YFYKTKHKR GGLHHHLRH GEDSIEAPD HPILKGVSL 
QHMIEMTTS GSGSGLPLL VQRSIARQI QLVEIIGQG 
RFGEVWRGR WRGENVAVK IFSSREERS WFREAEIYQ 
TVMLRHDNI LGFIAADNK DNGTWTQLW LITDYHEYG 
SLFDFLGRN TLDTTGMIR MALSIATGL AHLHMDIMG 
TQGKPAIAH RDLKSKNIL VKSNFTCAI GDLGLAVRH 
DATTDTVDI PLNNRVGTK RYMAPEVLD ETMNVNHFD 
SFKRADVYA LGLIFWEIA RRCNVGGIY DEYQLPFYD 
AVPSDPTIE EMRRVVCVD KQRPNIPNR WQSCEALRV 
MLKLMKECW YHNATARLT ALRIKKTLS NFRTSEELK 
M
    CDS sequence
    • >TC003240 RA
      atgcctcct aaaacacat cgcaacaac atggccaga 
actagcctc cttgtgttt tgttgtgct gtcttttgg 
ctgagccga ttggataac ggggttcaa gcacttaaa 
tgtaagtgc gataattgt aaggaaacc aactacacg 
tgccagacc gatgggtac tgctttaca tcgacttac 
ttgtccaaa tctggaggc gtcgaacat aattatcgg 
tgcctggat cgatccctt caagttcct cataataat 
cctttgctc tgccatcgg cagaaccct ctcaattct 
gaatttgtc atggagtgc tgcaaaaac gaagactac 
tgcaatgta cacctttca ccccgacta atgcaaaag 
ccccaagcg ccgaaattc tccaatttt acaacagcg 
gaaatcgtc ttgatgtca gtcatccca acaatgttg 
ttgtgtttc atcggagtt ggtgtttat tacttttac 
aaaacaaaa cataagcgt ggagggtta caccaccat 
ttacgtcac ggtgaagac agtattgaa gctccagat 
catcctatt ttaaagggt gtgagtttg caacatatg 
attgagatg acaacgagt ggtagtggc tcaggcttg 
cctttattg gtgcaacgt agcattgct cgccaaatc 
caattggtt gaaataatc gggcagggg aggtttggc 
gaggtgtgg agagggcgt tggaggggt gaaaatgtc 
gctgtgaaa attttcagt tcacgagag gaacgttca 
tggtttcgt gaagctgag atttatcaa accgttatg 
ttacgacat gataacatc ctcggtttt attgcggcc 
gataataaa gataatggg acatggacc caattgtgg 
ttgattacc gattatcac gaatatggt tcgcttttt 
gatttcctg ggtcgcaat actttggat accaccggt 
atgatacga atggcttta tctatagcc acaggtctt 
gcccatctc catatggat atcatgggt acgcagggc 
aagccagca attgcccat cgtgacttg aagtcaaag 
aatattttg gtcaagtcg aattttacg tgtgctatt 
ggtgatttg ggattagct gttagacat gatgcgaca 
actgatacc gttgatatt ccgctcaat aatcgagtg 
ggtacgaaa cggtatatg gcaccggaa gttttggac 
gaaacgatg aatgtaaat cattttgat tcgttcaaa 
cgagccgat gtttatgcg ttagggttg attttttgg 
gagattgcg aggcgatgt aatgtggga gggatttac 
gatgagtat cagttaccg ttttatgat gctgtgccg 
tctgatccc accattgaa gaaatgaga agggtggtg 
tgtgtcgat aaacaaagg cctaacata ccaaaccgt 
tggcagagt tgcgaagct ctccgcgtc atgttgaaa 
ctaatgaaa gagtgttgg tatcacaac gcgacagct 
cgtttaaca gctctaagg attaaaaag acactctct 
aacttccga acatcggag gaacttaaa atgtga
    • >TC003240 RB
      atgcctcct aaaacacat cgcaacaac atggccaga 
actagcctc cttgtgttt tgttgtgct gtcttttgg 
ctgagccga ttggataac ggggttcaa gcacttaaa 
tgtaagtgc gataattgt aaggaaacc aactacacg 
tgccagacc gatgggtac tgctttaca tcgacttac 
ttgtccaaa tctggaggc gtcgaacat aattatcgc 
tgtttagac aaacgccag tttttccca cctgaagaa 
ccccgatgg tgccacagc agacccacg acagaatac 
gttcaaagg tgcctggat cgatccctt caagttcct 
cataataat cctttgctc tgccatcgg cagaaccct 
ctcaattct gaatttgtc atggagtgc tgcaaaaac 
gaagactac tgcaatgta cacctttca ccccgacta 
atgcaaaag ccccaagcg ccgaaattc tccaatttt 
acaacagcg gaaatcgtc ttgatgtca gtcatccca 
acaatgttg ttgtgtttc atcggagtt ggtgtttat 
tacttttac aaaacaaaa cataagcgt ggagggtta 
caccaccat ttacgtcac ggtgaagac agtattgaa 
gctccagat catcctatt ttaaagggt gtgagtttg 
caacatatg attgagatg acaacgagt ggtagtggc 
tcaggcttg cctttattg gtgcaacgt agcattgct 
cgccaaatc caattggtt gaaataatc gggcagggg 
aggtttggc gaggtgtgg agagggcgt tggaggggt 
gaaaatgtc gctgtgaaa attttcagt tcacgagag 
gaacgttca tggtttcgt gaagctgag atttatcaa 
accgttatg ttacgacat gataacatc ctcggtttt 
attgcggcc gataataaa gataatggg acatggacc 
caattgtgg ttgattacc gattatcac gaatatggt 
tcgcttttt gatttcctg ggtcgcaat actttggat 
accaccggt atgatacga atggcttta tctatagcc 
acaggtctt gcccatctc catatggat atcatgggt 
acgcagggc aagccagca attgcccat cgtgacttg 
aagtcaaag aatattttg gtcaagtcg aattttacg 
tgtgctatt ggtgatttg ggattagct gttagacat 
gatgcgaca actgatacc gttgatatt ccgctcaat 
aatcgagtg ggtacgaaa cggtatatg gcaccggaa 
gttttggac gaaacgatg aatgtaaat cattttgat 
tcgttcaaa cgagccgat gtttatgcg ttagggttg 
attttttgg gagattgcg aggcgatgt aatgtggga 
gggatttac gatgagtat cagttaccg ttttatgat 
gctgtgccg tctgatccc accattgaa gaaatgaga 
agggtggtg tgtgtcgat aaacaaagg cctaacata 
ccaaaccgt tggcagagt tgcgaagct ctccgcgtc 
atgttgaaa ctaatgaaa gagtgttgg tatcacaac 
gcgacagct cgtttaaca gctctaagg attaaaaag 
acactctct aacttccga acatcggag gaacttaaa 
atgtga
    mRNA sequence
    • >TC003240 RA
      acatggctg atatttttg attcggtca ttctacttt 
ttaaataat ttttaattt tgtttaaaa aaaagtgtt 
gtttagttg cttgtcatg ctttggtgg ccccttggt 
ggtgcagcg taacactta tcgataatg tcagcgtcg 
tgcacaaag tggaagtgt aaacaatta tcaaataat 
tgaagtgtc ctcctaatg gcagccaag tgtacgttt 
ttcgccgaa tttttttgt aaataatta tgcaaatgt 
gcgcgaatt caactagac tttttgttg tttacgatg 
cctcctaaa acacatcgc aacaacatg gccagaact 
agcctcctt gtgttttgt tgtgctgtc ttttggctg 
agccgattg gataacggg gttcaagca cttaaatgt 
aagtgcgat aattgtaag gaaaccaac tacacgtgc 
cagaccgat gggtactgc tttacatcg acttacttg 
tccaaatct ggaggcgtc gaacataat tatcggtgc 
ctggatcga tcccttcaa gttcctcat aataatcct 
ttgctctgc catcggcag aaccctctc aattctgaa 
tttgtcatg gagtgctgc aaaaacgaa gactactgc 
aatgtacac ctttcaccc cgactaatg caaaagccc 
caagcgccg aaattctcc aattttaca acagcggaa 
atcgtcttg atgtcagtc atcccaaca atgttgttg 
tgtttcatc ggagttggt gtttattac ttttacaaa 
acaaaacat aagcgtgga gggttacac caccattta 
cgtcacggt gaagacagt attgaagct ccagatcat 
cctatttta aagggtgtg agtttgcaa catatgatt 
gagatgaca acgagtggt agtggctca ggcttgcct 
ttattggtg caacgtagc attgctcgc caaatccaa 
ttggttgaa ataatcggg caggggagg tttggcgag 
gtgtggaga gggcgttgg aggggtgaa aatgtcgct 
gtgaaaatt ttcagttca cgagaggaa cgttcatgg 
tttcgtgaa gctgagatt tatcaaacc gttatgtta 
cgacatgat aacatcctc ggttttatt gcggccgat 
aataaagat aatgggaca tggacccaa ttgtggttg 
attaccgat tatcacgaa tatggttcg ctttttgat 
ttcctgggt cgcaatact ttggatacc accggtatg 
atacgaatg gctttatct atagccaca ggtcttgcc 
catctccat atggatatc atgggtacg cagggcaag 
ccagcaatt gcccatcgt gacttgaag tcaaagaat 
attttggtc aagtcgaat tttacgtgt gctattggt 
gatttggga ttagctgtt agacatgat gcgacaact 
gataccgtt gatattccg ctcaataat cgagtgggt 
acgaaacgg tatatggca ccggaagtt ttggacgaa 
acgatgaat gtaaatcat tttgattcg ttcaaacga 
gccgatgtt tatgcgtta gggttgatt ttttgggag 
attgcgagg cgatgtaat gtgggaggg atttacgat 
gagtatcag ttaccgttt tatgatgct gtgccgtct 
gatcccacc attgaagaa atgagaagg gtggtgtgt 
gtcgataaa caaaggcct aacatacca aaccgttgg 
cagagttgc gaagctctc cgcgtcatg ttgaaacta 
atgaaagag tgttggtat cacaacgcg acagctcgt 
ttaacagct ctaaggatt aaaaagaca ctctctaac 
ttccgaaca tcggaggaa cttaaaatg tgaaagttt 
agcatgatt gtgtcaggt aaaacgaaa cgaaagcaa 
taattttta tttaataat taataatgt tttgttaca 
ggtgtacaa aacaatgtg tgaacgctc acgcgattt 
tttttttcg tttattttc cgagtatta caagtgttg 
taagccggt cctatgtga tagagcgtt gatttgtta 
aaaaaatgt ctcatagga tgtgatgaa aggtctgta 
tgaaagagg agtgaaatg ttcactgcc ccggtttgg 
ttcatatcc acaataata tacagtact gcctgccca 
cccattcca tagtttacg agagcttct tccttggtt 
ttgtgaccg gtaataatg tgatccttc ccgattctc 
tgcttgatc tctatttcc gttgattgt tataatctt 
tgatgaaat taactgccg acatgccaa agccataaa 
cagagtcaa ttaattttt acacaaaca agtaatagc 
tgttaaaga caatatttt ttattcaac tgttgaaat 
caaccgtta tagcggata aagtagaga tctttttat 
acatagtgt agatataat acgtaatag ttgtacatt 
tgttttgtt cggtttgat tcaaaaggc tcctagtca 
acaatagaa cagcagatt ctggcaaca cctgtgtga 
atgtggagt gaaaatttt cctagttcc tacaaatat 
ttggtggtt tttcgtttt gcaagcgtg taaaagttg 
ttgcatact atttttcag ttgtaaata ttttaattt 
tgactaagt gaggagaac gtaatttaa cgtgtataa 
gaatttaat tgtgctaac tatattttt aaggatatt 
tataaaaaa aataaattt actatgtaa tt
    • >TC003240 RB
      acatggctg atatttttg attcggtca ttctacttt 
ttaaataat ttttaattt tgtttaaaa aaaagtgtt 
gtttagttg cttgtcatg ctttggtgg ccccttggt 
ggtgcagcg taacactta tcgataatg tcagcgtcg 
tgcacaaag tggaagtgt aaacaatta tcaaataat 
tgaagtgtc ctcctaatg gcagccaag tgtacgttt 
ttcgccgaa tttttttgt aaataatta tgcaaatgt 
gcgcgaatt caactagac tttttgttg tttacgatg 
cctcctaaa acacatcgc aacaacatg gccagaact 
agcctcctt gtgttttgt tgtgctgtc ttttggctg 
agccgattg gataacggg gttcaagca cttaaatgt 
aagtgcgat aattgtaag gaaaccaac tacacgtgc 
cagaccgat gggtactgc tttacatcg acttacttg 
tccaaatct ggaggcgtc gaacataat tatcgctgt 
ttagacaaa cgccagttt ttcccacct gaagaaccc 
cgatggtgc cacagcaga cccacgaca gaatacgtt 
caaaggtgc ctggatcga tcccttcaa gttcctcat 
aataatcct ttgctctgc catcggcag aaccctctc 
aattctgaa tttgtcatg gagtgctgc aaaaacgaa 
gactactgc aatgtacac ctttcaccc cgactaatg 
caaaagccc caagcgccg aaattctcc aattttaca 
acagcggaa atcgtcttg atgtcagtc atcccaaca 
atgttgttg tgtttcatc ggagttggt gtttattac 
ttttacaaa acaaaacat aagcgtgga gggttacac 
caccattta cgtcacggt gaagacagt attgaagct 
ccagatcat cctatttta aagggtgtg agtttgcaa 
catatgatt gagatgaca acgagtggt agtggctca 
ggcttgcct ttattggtg caacgtagc attgctcgc 
caaatccaa ttggttgaa ataatcggg caggggagg 
tttggcgag gtgtggaga gggcgttgg aggggtgaa 
aatgtcgct gtgaaaatt ttcagttca cgagaggaa 
cgttcatgg tttcgtgaa gctgagatt tatcaaacc 
gttatgtta cgacatgat aacatcctc ggttttatt 
gcggccgat aataaagat aatgggaca tggacccaa 
ttgtggttg attaccgat tatcacgaa tatggttcg 
ctttttgat ttcctgggt cgcaatact ttggatacc 
accggtatg atacgaatg gctttatct atagccaca 
ggtcttgcc catctccat atggatatc atgggtacg 
cagggcaag ccagcaatt gcccatcgt gacttgaag 
tcaaagaat attttggtc aagtcgaat tttacgtgt 
gctattggt gatttggga ttagctgtt agacatgat 
gcgacaact gataccgtt gatattccg ctcaataat 
cgagtgggt acgaaacgg tatatggca ccggaagtt 
ttggacgaa acgatgaat gtaaatcat tttgattcg 
ttcaaacga gccgatgtt tatgcgtta gggttgatt 
ttttgggag attgcgagg cgatgtaat gtgggaggg 
atttacgat gagtatcag ttaccgttt tatgatgct 
gtgccgtct gatcccacc attgaagaa atgagaagg 
gtggtgtgt gtcgataaa caaaggcct aacatacca 
aaccgttgg cagagttgc gaagctctc cgcgtcatg 
ttgaaacta atgaaagag tgttggtat cacaacgcg 
acagctcgt ttaacagct ctaaggatt aaaaagaca 
ctctctaac ttccgaaca tcggaggaa cttaaaatg 
tgaaagttt agcatgatt gtgtcaggt aaaacgaaa 
cgaaagcaa taattttta tttaataat taataatgt 
tttgttaca ggtgtacaa aacaatgtg tgaacgctc 
acgcgattt tttttttcg tttattttc cgagtatta 
caagtgttg taagccggt cctatgtga tagagcgtt 
gatttgtta aaaaaatgt ctcatagga tgtgatgaa 
aggtctgta tgaaagagg agtgaaatg ttcactgcc 
ccggtttgg ttcatatcc acaataata tacagtact 
gcctgccca cccattcca tagtttacg agagcttct 
tccttggtt ttgtgaccg gtaataatg tgatccttc 
ccgattctc tgcttgatc tctatttcc gttgattgt 
tataatctt tgatgaaat taactgccg acatgccaa 
agccataaa cagagtcaa ttaattttt acacaaaca 
agtaatagc tgttaaaga caatatttt ttattcaac 
tgttgaaat caaccgtta tagcggata aagtagaga 
tctttttat acatagtgt agatataat acgtaatag 
ttgtacatt tgttttgtt cggtttgat tcaaaaggc 
tcctagtca acaatagaa cagcagatt ctggcaaca 
cctgtgtga atgtggagt gaaaatttt cctagttcc 
tacaaatat ttggtggtt tttcgtttt gcaagcgtg 
taaaagttg ttgcatact atttttcag ttgtaaata 
ttttaattt tgactaagt gaggagaac gtaatttaa 
cgtgtataa gaatttaat tgtgctaac tatattttt 
aaggatatt tataaaaaa aataaattt actatgtaa 
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