| General information | |||
| TC003240 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid13: 199797..206593 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003240 | |
| General information | ||||
| iB_03525 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (19 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (22 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (41 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (41 dpi)
- >TC003240 RA
MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR CLDRSLQVP HNNPLLCHR QNPLNSEFV MECCKNEDY CNVHLSPRL MQKPQAPKF SNFTTAEIV LMSVIPTML LCFIGVGVY YFYKTKHKR GGLHHHLRH GEDSIEAPD HPILKGVSL QHMIEMTTS GSGSGLPLL VQRSIARQI QLVEIIGQG RFGEVWRGR WRGENVAVK IFSSREERS WFREAEIYQ TVMLRHDNI LGFIAADNK DNGTWTQLW LITDYHEYG SLFDFLGRN TLDTTGMIR MALSIATGL AHLHMDIMG TQGKPAIAH RDLKSKNIL VKSNFTCAI GDLGLAVRH DATTDTVDI PLNNRVGTK RYMAPEVLD ETMNVNHFD SFKRADVYA LGLIFWEIA RRCNVGGIY DEYQLPFYD AVPSDPTIE EMRRVVCVD KQRPNIPNR WQSCEALRV MLKLMKECW YHNATARLT ALRIKKTLS NFRTSEELK M
- >TC003240 RB
MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR CLDKRQFFP PEEPRWCHS RPTTEYVQR CLDRSLQVP HNNPLLCHR QNPLNSEFV MECCKNEDY CNVHLSPRL MQKPQAPKF SNFTTAEIV LMSVIPTML LCFIGVGVY YFYKTKHKR GGLHHHLRH GEDSIEAPD HPILKGVSL QHMIEMTTS GSGSGLPLL VQRSIARQI QLVEIIGQG RFGEVWRGR WRGENVAVK IFSSREERS WFREAEIYQ TVMLRHDNI LGFIAADNK DNGTWTQLW LITDYHEYG SLFDFLGRN TLDTTGMIR MALSIATGL AHLHMDIMG TQGKPAIAH RDLKSKNIL VKSNFTCAI GDLGLAVRH DATTDTVDI PLNNRVGTK RYMAPEVLD ETMNVNHFD SFKRADVYA LGLIFWEIA RRCNVGGIY DEYQLPFYD AVPSDPTIE EMRRVVCVD KQRPNIPNR WQSCEALRV MLKLMKECW YHNATARLT ALRIKKTLS NFRTSEELK M
- >TC003240 RA
atgcctcct aaaacacat cgcaacaac atggccaga actagcctc cttgtgttt tgttgtgct gtcttttgg ctgagccga ttggataac ggggttcaa gcacttaaa tgtaagtgc gataattgt aaggaaacc aactacacg tgccagacc gatgggtac tgctttaca tcgacttac ttgtccaaa tctggaggc gtcgaacat aattatcgg tgcctggat cgatccctt caagttcct cataataat cctttgctc tgccatcgg cagaaccct ctcaattct gaatttgtc atggagtgc tgcaaaaac gaagactac tgcaatgta cacctttca ccccgacta atgcaaaag ccccaagcg ccgaaattc tccaatttt acaacagcg gaaatcgtc ttgatgtca gtcatccca acaatgttg ttgtgtttc atcggagtt ggtgtttat tacttttac aaaacaaaa cataagcgt ggagggtta caccaccat ttacgtcac ggtgaagac agtattgaa gctccagat catcctatt ttaaagggt gtgagtttg caacatatg attgagatg acaacgagt ggtagtggc tcaggcttg cctttattg gtgcaacgt agcattgct cgccaaatc caattggtt gaaataatc gggcagggg aggtttggc gaggtgtgg agagggcgt tggaggggt gaaaatgtc gctgtgaaa attttcagt tcacgagag gaacgttca tggtttcgt gaagctgag atttatcaa accgttatg ttacgacat gataacatc ctcggtttt attgcggcc gataataaa gataatggg acatggacc caattgtgg ttgattacc gattatcac gaatatggt tcgcttttt gatttcctg ggtcgcaat actttggat accaccggt atgatacga atggcttta tctatagcc acaggtctt gcccatctc catatggat atcatgggt acgcagggc aagccagca attgcccat cgtgacttg aagtcaaag aatattttg gtcaagtcg aattttacg tgtgctatt ggtgatttg ggattagct gttagacat gatgcgaca actgatacc gttgatatt ccgctcaat aatcgagtg ggtacgaaa cggtatatg gcaccggaa gttttggac gaaacgatg aatgtaaat cattttgat tcgttcaaa cgagccgat gtttatgcg ttagggttg attttttgg gagattgcg aggcgatgt aatgtggga gggatttac gatgagtat cagttaccg ttttatgat gctgtgccg tctgatccc accattgaa gaaatgaga agggtggtg tgtgtcgat aaacaaagg cctaacata ccaaaccgt tggcagagt tgcgaagct ctccgcgtc atgttgaaa ctaatgaaa gagtgttgg tatcacaac gcgacagct cgtttaaca gctctaagg attaaaaag acactctct aacttccga acatcggag gaacttaaa atgtga
- >TC003240 RB
atgcctcct aaaacacat cgcaacaac atggccaga actagcctc cttgtgttt tgttgtgct gtcttttgg ctgagccga ttggataac ggggttcaa gcacttaaa tgtaagtgc gataattgt aaggaaacc aactacacg tgccagacc gatgggtac tgctttaca tcgacttac ttgtccaaa tctggaggc gtcgaacat aattatcgc tgtttagac aaacgccag tttttccca cctgaagaa ccccgatgg tgccacagc agacccacg acagaatac gttcaaagg tgcctggat cgatccctt caagttcct cataataat cctttgctc tgccatcgg cagaaccct ctcaattct gaatttgtc atggagtgc tgcaaaaac gaagactac tgcaatgta cacctttca ccccgacta atgcaaaag ccccaagcg ccgaaattc tccaatttt acaacagcg gaaatcgtc ttgatgtca gtcatccca acaatgttg ttgtgtttc atcggagtt ggtgtttat tacttttac aaaacaaaa cataagcgt ggagggtta caccaccat ttacgtcac ggtgaagac agtattgaa gctccagat catcctatt ttaaagggt gtgagtttg caacatatg attgagatg acaacgagt ggtagtggc tcaggcttg cctttattg gtgcaacgt agcattgct cgccaaatc caattggtt gaaataatc gggcagggg aggtttggc gaggtgtgg agagggcgt tggaggggt gaaaatgtc gctgtgaaa attttcagt tcacgagag gaacgttca tggtttcgt gaagctgag atttatcaa accgttatg ttacgacat gataacatc ctcggtttt attgcggcc gataataaa gataatggg acatggacc caattgtgg ttgattacc gattatcac gaatatggt tcgcttttt gatttcctg ggtcgcaat actttggat accaccggt atgatacga atggcttta tctatagcc acaggtctt gcccatctc catatggat atcatgggt acgcagggc aagccagca attgcccat cgtgacttg aagtcaaag aatattttg gtcaagtcg aattttacg tgtgctatt ggtgatttg ggattagct gttagacat gatgcgaca actgatacc gttgatatt ccgctcaat aatcgagtg ggtacgaaa cggtatatg gcaccggaa gttttggac gaaacgatg aatgtaaat cattttgat tcgttcaaa cgagccgat gtttatgcg ttagggttg attttttgg gagattgcg aggcgatgt aatgtggga gggatttac gatgagtat cagttaccg ttttatgat gctgtgccg tctgatccc accattgaa gaaatgaga agggtggtg tgtgtcgat aaacaaagg cctaacata ccaaaccgt tggcagagt tgcgaagct ctccgcgtc atgttgaaa ctaatgaaa gagtgttgg tatcacaac gcgacagct cgtttaaca gctctaagg attaaaaag acactctct aacttccga acatcggag gaacttaaa atgtga
- >TC003240 RA
acatggctg atatttttg attcggtca ttctacttt ttaaataat ttttaattt tgtttaaaa aaaagtgtt gtttagttg cttgtcatg ctttggtgg ccccttggt ggtgcagcg taacactta tcgataatg tcagcgtcg tgcacaaag tggaagtgt aaacaatta tcaaataat tgaagtgtc ctcctaatg gcagccaag tgtacgttt ttcgccgaa tttttttgt aaataatta tgcaaatgt gcgcgaatt caactagac tttttgttg tttacgatg cctcctaaa acacatcgc aacaacatg gccagaact agcctcctt gtgttttgt tgtgctgtc ttttggctg agccgattg gataacggg gttcaagca cttaaatgt aagtgcgat aattgtaag gaaaccaac tacacgtgc cagaccgat gggtactgc tttacatcg acttacttg tccaaatct ggaggcgtc gaacataat tatcggtgc ctggatcga tcccttcaa gttcctcat aataatcct ttgctctgc catcggcag aaccctctc aattctgaa tttgtcatg gagtgctgc aaaaacgaa gactactgc aatgtacac ctttcaccc cgactaatg caaaagccc caagcgccg aaattctcc aattttaca acagcggaa atcgtcttg atgtcagtc atcccaaca atgttgttg tgtttcatc ggagttggt gtttattac ttttacaaa acaaaacat aagcgtgga gggttacac caccattta cgtcacggt gaagacagt attgaagct ccagatcat cctatttta aagggtgtg agtttgcaa catatgatt gagatgaca acgagtggt agtggctca ggcttgcct ttattggtg caacgtagc attgctcgc caaatccaa ttggttgaa ataatcggg caggggagg tttggcgag gtgtggaga gggcgttgg aggggtgaa aatgtcgct gtgaaaatt ttcagttca cgagaggaa cgttcatgg tttcgtgaa gctgagatt tatcaaacc gttatgtta cgacatgat aacatcctc ggttttatt gcggccgat aataaagat aatgggaca tggacccaa ttgtggttg attaccgat tatcacgaa tatggttcg ctttttgat ttcctgggt cgcaatact ttggatacc accggtatg atacgaatg gctttatct atagccaca ggtcttgcc catctccat atggatatc atgggtacg cagggcaag ccagcaatt gcccatcgt gacttgaag tcaaagaat attttggtc aagtcgaat tttacgtgt gctattggt gatttggga ttagctgtt agacatgat gcgacaact gataccgtt gatattccg ctcaataat cgagtgggt acgaaacgg tatatggca ccggaagtt ttggacgaa acgatgaat gtaaatcat tttgattcg ttcaaacga gccgatgtt tatgcgtta gggttgatt ttttgggag attgcgagg cgatgtaat gtgggaggg atttacgat gagtatcag ttaccgttt tatgatgct gtgccgtct gatcccacc attgaagaa atgagaagg gtggtgtgt gtcgataaa caaaggcct aacatacca aaccgttgg cagagttgc gaagctctc cgcgtcatg ttgaaacta atgaaagag tgttggtat cacaacgcg acagctcgt ttaacagct ctaaggatt aaaaagaca ctctctaac ttccgaaca tcggaggaa cttaaaatg tgaaagttt agcatgatt gtgtcaggt aaaacgaaa cgaaagcaa taattttta tttaataat taataatgt tttgttaca ggtgtacaa aacaatgtg tgaacgctc acgcgattt tttttttcg tttattttc cgagtatta caagtgttg taagccggt cctatgtga tagagcgtt gatttgtta aaaaaatgt ctcatagga tgtgatgaa aggtctgta tgaaagagg agtgaaatg ttcactgcc ccggtttgg ttcatatcc acaataata tacagtact gcctgccca cccattcca tagtttacg agagcttct tccttggtt ttgtgaccg gtaataatg tgatccttc ccgattctc tgcttgatc tctatttcc gttgattgt tataatctt tgatgaaat taactgccg acatgccaa agccataaa cagagtcaa ttaattttt acacaaaca agtaatagc tgttaaaga caatatttt ttattcaac tgttgaaat caaccgtta tagcggata aagtagaga tctttttat acatagtgt agatataat acgtaatag ttgtacatt tgttttgtt cggtttgat tcaaaaggc tcctagtca acaatagaa cagcagatt ctggcaaca cctgtgtga atgtggagt gaaaatttt cctagttcc tacaaatat ttggtggtt tttcgtttt gcaagcgtg taaaagttg ttgcatact atttttcag ttgtaaata ttttaattt tgactaagt gaggagaac gtaatttaa cgtgtataa gaatttaat tgtgctaac tatattttt aaggatatt tataaaaaa aataaattt actatgtaa tt
- >TC003240 RB
acatggctg atatttttg attcggtca ttctacttt ttaaataat ttttaattt tgtttaaaa aaaagtgtt gtttagttg cttgtcatg ctttggtgg ccccttggt ggtgcagcg taacactta tcgataatg tcagcgtcg tgcacaaag tggaagtgt aaacaatta tcaaataat tgaagtgtc ctcctaatg gcagccaag tgtacgttt ttcgccgaa tttttttgt aaataatta tgcaaatgt gcgcgaatt caactagac tttttgttg tttacgatg cctcctaaa acacatcgc aacaacatg gccagaact agcctcctt gtgttttgt tgtgctgtc ttttggctg agccgattg gataacggg gttcaagca cttaaatgt aagtgcgat aattgtaag gaaaccaac tacacgtgc cagaccgat gggtactgc tttacatcg acttacttg tccaaatct ggaggcgtc gaacataat tatcgctgt ttagacaaa cgccagttt ttcccacct gaagaaccc cgatggtgc cacagcaga cccacgaca gaatacgtt caaaggtgc ctggatcga tcccttcaa gttcctcat aataatcct ttgctctgc catcggcag aaccctctc aattctgaa tttgtcatg gagtgctgc aaaaacgaa gactactgc aatgtacac ctttcaccc cgactaatg caaaagccc caagcgccg aaattctcc aattttaca acagcggaa atcgtcttg atgtcagtc atcccaaca atgttgttg tgtttcatc ggagttggt gtttattac ttttacaaa acaaaacat aagcgtgga gggttacac caccattta cgtcacggt gaagacagt attgaagct ccagatcat cctatttta aagggtgtg agtttgcaa catatgatt gagatgaca acgagtggt agtggctca ggcttgcct ttattggtg caacgtagc attgctcgc caaatccaa ttggttgaa ataatcggg caggggagg tttggcgag gtgtggaga gggcgttgg aggggtgaa aatgtcgct gtgaaaatt ttcagttca cgagaggaa cgttcatgg tttcgtgaa gctgagatt tatcaaacc gttatgtta cgacatgat aacatcctc ggttttatt gcggccgat aataaagat aatgggaca tggacccaa ttgtggttg attaccgat tatcacgaa tatggttcg ctttttgat ttcctgggt cgcaatact ttggatacc accggtatg atacgaatg gctttatct atagccaca ggtcttgcc catctccat atggatatc atgggtacg cagggcaag ccagcaatt gcccatcgt gacttgaag tcaaagaat attttggtc aagtcgaat tttacgtgt gctattggt gatttggga ttagctgtt agacatgat gcgacaact gataccgtt gatattccg ctcaataat cgagtgggt acgaaacgg tatatggca ccggaagtt ttggacgaa acgatgaat gtaaatcat tttgattcg ttcaaacga gccgatgtt tatgcgtta gggttgatt ttttgggag attgcgagg cgatgtaat gtgggaggg atttacgat gagtatcag ttaccgttt tatgatgct gtgccgtct gatcccacc attgaagaa atgagaagg gtggtgtgt gtcgataaa caaaggcct aacatacca aaccgttgg cagagttgc gaagctctc cgcgtcatg ttgaaacta atgaaagag tgttggtat cacaacgcg acagctcgt ttaacagct ctaaggatt aaaaagaca ctctctaac ttccgaaca tcggaggaa cttaaaatg tgaaagttt agcatgatt gtgtcaggt aaaacgaaa cgaaagcaa taattttta tttaataat taataatgt tttgttaca ggtgtacaa aacaatgtg tgaacgctc acgcgattt tttttttcg tttattttc cgagtatta caagtgttg taagccggt cctatgtga tagagcgtt gatttgtta aaaaaatgt ctcatagga tgtgatgaa aggtctgta tgaaagagg agtgaaatg ttcactgcc ccggtttgg ttcatatcc acaataata tacagtact gcctgccca cccattcca tagtttacg agagcttct tccttggtt ttgtgaccg gtaataatg tgatccttc ccgattctc tgcttgatc tctatttcc gttgattgt tataatctt tgatgaaat taactgccg acatgccaa agccataaa cagagtcaa ttaattttt acacaaaca agtaatagc tgttaaaga caatatttt ttattcaac tgttgaaat caaccgtta tagcggata aagtagaga tctttttat acatagtgt agatataat acgtaatag ttgtacatt tgttttgtt cggtttgat tcaaaaggc tcctagtca acaatagaa cagcagatt ctggcaaca cctgtgtga atgtggagt gaaaatttt cctagttcc tacaaatat ttggtggtt tttcgtttt gcaagcgtg taaaagttg ttgcatact atttttcag ttgtaaata ttttaattt tgactaagt gaggagaac gtaatttaa cgtgtataa gaatttaat tgtgctaac tatattttt aaggatatt tataaaaaa aataaattt actatgtaa tt