Status
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Gene information
General information
TC003240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 199797..206593
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003240
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (22 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003240 RA
      MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK 
      CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR 
      CLDRSLQVP HNNPLLCHR QNPLNSEFV MECCKNEDY 
      CNVHLSPRL MQKPQAPKF SNFTTAEIV LMSVIPTML 
      LCFIGVGVY YFYKTKHKR GGLHHHLRH GEDSIEAPD 
      HPILKGVSL QHMIEMTTS GSGSGLPLL VQRSIARQI 
      QLVEIIGQG RFGEVWRGR WRGENVAVK IFSSREERS 
      WFREAEIYQ TVMLRHDNI LGFIAADNK DNGTWTQLW 
      LITDYHEYG SLFDFLGRN TLDTTGMIR MALSIATGL 
      AHLHMDIMG TQGKPAIAH RDLKSKNIL VKSNFTCAI 
      GDLGLAVRH DATTDTVDI PLNNRVGTK RYMAPEVLD 
      ETMNVNHFD SFKRADVYA LGLIFWEIA RRCNVGGIY 
      DEYQLPFYD AVPSDPTIE EMRRVVCVD KQRPNIPNR 
      WQSCEALRV MLKLMKECW YHNATARLT ALRIKKTLS 
      NFRTSEELK M
    • >TC003240 RB
      MPPKTHRNN MARTSLLVF CCAVFWLSR LDNGVQALK 
      CKCDNCKET NYTCQTDGY CFTSTYLSK SGGVEHNYR 
      CLDKRQFFP PEEPRWCHS RPTTEYVQR CLDRSLQVP 
      HNNPLLCHR QNPLNSEFV MECCKNEDY CNVHLSPRL 
      MQKPQAPKF SNFTTAEIV LMSVIPTML LCFIGVGVY 
      YFYKTKHKR GGLHHHLRH GEDSIEAPD HPILKGVSL 
      QHMIEMTTS GSGSGLPLL VQRSIARQI QLVEIIGQG 
      RFGEVWRGR WRGENVAVK IFSSREERS WFREAEIYQ 
      TVMLRHDNI LGFIAADNK DNGTWTQLW LITDYHEYG 
      SLFDFLGRN TLDTTGMIR MALSIATGL AHLHMDIMG 
      TQGKPAIAH RDLKSKNIL VKSNFTCAI GDLGLAVRH 
      DATTDTVDI PLNNRVGTK RYMAPEVLD ETMNVNHFD 
      SFKRADVYA LGLIFWEIA RRCNVGGIY DEYQLPFYD 
      AVPSDPTIE EMRRVVCVD KQRPNIPNR WQSCEALRV 
      MLKLMKECW YHNATARLT ALRIKKTLS NFRTSEELK 
      M
    CDS sequence
    • >TC003240 RA
      atgcctcct aaaacacat cgcaacaac atggccaga 
      actagcctc cttgtgttt tgttgtgct gtcttttgg 
      ctgagccga ttggataac ggggttcaa gcacttaaa 
      tgtaagtgc gataattgt aaggaaacc aactacacg 
      tgccagacc gatgggtac tgctttaca tcgacttac 
      ttgtccaaa tctggaggc gtcgaacat aattatcgg 
      tgcctggat cgatccctt caagttcct cataataat 
      cctttgctc tgccatcgg cagaaccct ctcaattct 
      gaatttgtc atggagtgc tgcaaaaac gaagactac 
      tgcaatgta cacctttca ccccgacta atgcaaaag 
      ccccaagcg ccgaaattc tccaatttt acaacagcg 
      gaaatcgtc ttgatgtca gtcatccca acaatgttg 
      ttgtgtttc atcggagtt ggtgtttat tacttttac 
      aaaacaaaa cataagcgt ggagggtta caccaccat 
      ttacgtcac ggtgaagac agtattgaa gctccagat 
      catcctatt ttaaagggt gtgagtttg caacatatg 
      attgagatg acaacgagt ggtagtggc tcaggcttg 
      cctttattg gtgcaacgt agcattgct cgccaaatc 
      caattggtt gaaataatc gggcagggg aggtttggc 
      gaggtgtgg agagggcgt tggaggggt gaaaatgtc 
      gctgtgaaa attttcagt tcacgagag gaacgttca 
      tggtttcgt gaagctgag atttatcaa accgttatg 
      ttacgacat gataacatc ctcggtttt attgcggcc 
      gataataaa gataatggg acatggacc caattgtgg 
      ttgattacc gattatcac gaatatggt tcgcttttt 
      gatttcctg ggtcgcaat actttggat accaccggt 
      atgatacga atggcttta tctatagcc acaggtctt 
      gcccatctc catatggat atcatgggt acgcagggc 
      aagccagca attgcccat cgtgacttg aagtcaaag 
      aatattttg gtcaagtcg aattttacg tgtgctatt 
      ggtgatttg ggattagct gttagacat gatgcgaca 
      actgatacc gttgatatt ccgctcaat aatcgagtg 
      ggtacgaaa cggtatatg gcaccggaa gttttggac 
      gaaacgatg aatgtaaat cattttgat tcgttcaaa 
      cgagccgat gtttatgcg ttagggttg attttttgg 
      gagattgcg aggcgatgt aatgtggga gggatttac 
      gatgagtat cagttaccg ttttatgat gctgtgccg 
      tctgatccc accattgaa gaaatgaga agggtggtg 
      tgtgtcgat aaacaaagg cctaacata ccaaaccgt 
      tggcagagt tgcgaagct ctccgcgtc atgttgaaa 
      ctaatgaaa gagtgttgg tatcacaac gcgacagct 
      cgtttaaca gctctaagg attaaaaag acactctct 
      aacttccga acatcggag gaacttaaa atgtga
    • >TC003240 RB
      atgcctcct aaaacacat cgcaacaac atggccaga 
      actagcctc cttgtgttt tgttgtgct gtcttttgg 
      ctgagccga ttggataac ggggttcaa gcacttaaa 
      tgtaagtgc gataattgt aaggaaacc aactacacg 
      tgccagacc gatgggtac tgctttaca tcgacttac 
      ttgtccaaa tctggaggc gtcgaacat aattatcgc 
      tgtttagac aaacgccag tttttccca cctgaagaa 
      ccccgatgg tgccacagc agacccacg acagaatac 
      gttcaaagg tgcctggat cgatccctt caagttcct 
      cataataat cctttgctc tgccatcgg cagaaccct 
      ctcaattct gaatttgtc atggagtgc tgcaaaaac 
      gaagactac tgcaatgta cacctttca ccccgacta 
      atgcaaaag ccccaagcg ccgaaattc tccaatttt 
      acaacagcg gaaatcgtc ttgatgtca gtcatccca 
      acaatgttg ttgtgtttc atcggagtt ggtgtttat 
      tacttttac aaaacaaaa cataagcgt ggagggtta 
      caccaccat ttacgtcac ggtgaagac agtattgaa 
      gctccagat catcctatt ttaaagggt gtgagtttg 
      caacatatg attgagatg acaacgagt ggtagtggc 
      tcaggcttg cctttattg gtgcaacgt agcattgct 
      cgccaaatc caattggtt gaaataatc gggcagggg 
      aggtttggc gaggtgtgg agagggcgt tggaggggt 
      gaaaatgtc gctgtgaaa attttcagt tcacgagag 
      gaacgttca tggtttcgt gaagctgag atttatcaa 
      accgttatg ttacgacat gataacatc ctcggtttt 
      attgcggcc gataataaa gataatggg acatggacc 
      caattgtgg ttgattacc gattatcac gaatatggt 
      tcgcttttt gatttcctg ggtcgcaat actttggat 
      accaccggt atgatacga atggcttta tctatagcc 
      acaggtctt gcccatctc catatggat atcatgggt 
      acgcagggc aagccagca attgcccat cgtgacttg 
      aagtcaaag aatattttg gtcaagtcg aattttacg 
      tgtgctatt ggtgatttg ggattagct gttagacat 
      gatgcgaca actgatacc gttgatatt ccgctcaat 
      aatcgagtg ggtacgaaa cggtatatg gcaccggaa 
      gttttggac gaaacgatg aatgtaaat cattttgat 
      tcgttcaaa cgagccgat gtttatgcg ttagggttg 
      attttttgg gagattgcg aggcgatgt aatgtggga 
      gggatttac gatgagtat cagttaccg ttttatgat 
      gctgtgccg tctgatccc accattgaa gaaatgaga 
      agggtggtg tgtgtcgat aaacaaagg cctaacata 
      ccaaaccgt tggcagagt tgcgaagct ctccgcgtc 
      atgttgaaa ctaatgaaa gagtgttgg tatcacaac 
      gcgacagct cgtttaaca gctctaagg attaaaaag 
      acactctct aacttccga acatcggag gaacttaaa 
      atgtga
    mRNA sequence
    • >TC003240 RA
      acatggctg atatttttg attcggtca ttctacttt 
      ttaaataat ttttaattt tgtttaaaa aaaagtgtt 
      gtttagttg cttgtcatg ctttggtgg ccccttggt 
      ggtgcagcg taacactta tcgataatg tcagcgtcg 
      tgcacaaag tggaagtgt aaacaatta tcaaataat 
      tgaagtgtc ctcctaatg gcagccaag tgtacgttt 
      ttcgccgaa tttttttgt aaataatta tgcaaatgt 
      gcgcgaatt caactagac tttttgttg tttacgatg 
      cctcctaaa acacatcgc aacaacatg gccagaact 
      agcctcctt gtgttttgt tgtgctgtc ttttggctg 
      agccgattg gataacggg gttcaagca cttaaatgt 
      aagtgcgat aattgtaag gaaaccaac tacacgtgc 
      cagaccgat gggtactgc tttacatcg acttacttg 
      tccaaatct ggaggcgtc gaacataat tatcggtgc 
      ctggatcga tcccttcaa gttcctcat aataatcct 
      ttgctctgc catcggcag aaccctctc aattctgaa 
      tttgtcatg gagtgctgc aaaaacgaa gactactgc 
      aatgtacac ctttcaccc cgactaatg caaaagccc 
      caagcgccg aaattctcc aattttaca acagcggaa 
      atcgtcttg atgtcagtc atcccaaca atgttgttg 
      tgtttcatc ggagttggt gtttattac ttttacaaa 
      acaaaacat aagcgtgga gggttacac caccattta 
      cgtcacggt gaagacagt attgaagct ccagatcat 
      cctatttta aagggtgtg agtttgcaa catatgatt 
      gagatgaca acgagtggt agtggctca ggcttgcct 
      ttattggtg caacgtagc attgctcgc caaatccaa 
      ttggttgaa ataatcggg caggggagg tttggcgag 
      gtgtggaga gggcgttgg aggggtgaa aatgtcgct 
      gtgaaaatt ttcagttca cgagaggaa cgttcatgg 
      tttcgtgaa gctgagatt tatcaaacc gttatgtta 
      cgacatgat aacatcctc ggttttatt gcggccgat 
      aataaagat aatgggaca tggacccaa ttgtggttg 
      attaccgat tatcacgaa tatggttcg ctttttgat 
      ttcctgggt cgcaatact ttggatacc accggtatg 
      atacgaatg gctttatct atagccaca ggtcttgcc 
      catctccat atggatatc atgggtacg cagggcaag 
      ccagcaatt gcccatcgt gacttgaag tcaaagaat 
      attttggtc aagtcgaat tttacgtgt gctattggt 
      gatttggga ttagctgtt agacatgat gcgacaact 
      gataccgtt gatattccg ctcaataat cgagtgggt 
      acgaaacgg tatatggca ccggaagtt ttggacgaa 
      acgatgaat gtaaatcat tttgattcg ttcaaacga 
      gccgatgtt tatgcgtta gggttgatt ttttgggag 
      attgcgagg cgatgtaat gtgggaggg atttacgat 
      gagtatcag ttaccgttt tatgatgct gtgccgtct 
      gatcccacc attgaagaa atgagaagg gtggtgtgt 
      gtcgataaa caaaggcct aacatacca aaccgttgg 
      cagagttgc gaagctctc cgcgtcatg ttgaaacta 
      atgaaagag tgttggtat cacaacgcg acagctcgt 
      ttaacagct ctaaggatt aaaaagaca ctctctaac 
      ttccgaaca tcggaggaa cttaaaatg tgaaagttt 
      agcatgatt gtgtcaggt aaaacgaaa cgaaagcaa 
      taattttta tttaataat taataatgt tttgttaca 
      ggtgtacaa aacaatgtg tgaacgctc acgcgattt 
      tttttttcg tttattttc cgagtatta caagtgttg 
      taagccggt cctatgtga tagagcgtt gatttgtta 
      aaaaaatgt ctcatagga tgtgatgaa aggtctgta 
      tgaaagagg agtgaaatg ttcactgcc ccggtttgg 
      ttcatatcc acaataata tacagtact gcctgccca 
      cccattcca tagtttacg agagcttct tccttggtt 
      ttgtgaccg gtaataatg tgatccttc ccgattctc 
      tgcttgatc tctatttcc gttgattgt tataatctt 
      tgatgaaat taactgccg acatgccaa agccataaa 
      cagagtcaa ttaattttt acacaaaca agtaatagc 
      tgttaaaga caatatttt ttattcaac tgttgaaat 
      caaccgtta tagcggata aagtagaga tctttttat 
      acatagtgt agatataat acgtaatag ttgtacatt 
      tgttttgtt cggtttgat tcaaaaggc tcctagtca 
      acaatagaa cagcagatt ctggcaaca cctgtgtga 
      atgtggagt gaaaatttt cctagttcc tacaaatat 
      ttggtggtt tttcgtttt gcaagcgtg taaaagttg 
      ttgcatact atttttcag ttgtaaata ttttaattt 
      tgactaagt gaggagaac gtaatttaa cgtgtataa 
      gaatttaat tgtgctaac tatattttt aaggatatt 
      tataaaaaa aataaattt actatgtaa tt
    • >TC003240 RB
      acatggctg atatttttg attcggtca ttctacttt 
      ttaaataat ttttaattt tgtttaaaa aaaagtgtt 
      gtttagttg cttgtcatg ctttggtgg ccccttggt 
      ggtgcagcg taacactta tcgataatg tcagcgtcg 
      tgcacaaag tggaagtgt aaacaatta tcaaataat 
      tgaagtgtc ctcctaatg gcagccaag tgtacgttt 
      ttcgccgaa tttttttgt aaataatta tgcaaatgt 
      gcgcgaatt caactagac tttttgttg tttacgatg 
      cctcctaaa acacatcgc aacaacatg gccagaact 
      agcctcctt gtgttttgt tgtgctgtc ttttggctg 
      agccgattg gataacggg gttcaagca cttaaatgt 
      aagtgcgat aattgtaag gaaaccaac tacacgtgc 
      cagaccgat gggtactgc tttacatcg acttacttg 
      tccaaatct ggaggcgtc gaacataat tatcgctgt 
      ttagacaaa cgccagttt ttcccacct gaagaaccc 
      cgatggtgc cacagcaga cccacgaca gaatacgtt 
      caaaggtgc ctggatcga tcccttcaa gttcctcat 
      aataatcct ttgctctgc catcggcag aaccctctc 
      aattctgaa tttgtcatg gagtgctgc aaaaacgaa 
      gactactgc aatgtacac ctttcaccc cgactaatg 
      caaaagccc caagcgccg aaattctcc aattttaca 
      acagcggaa atcgtcttg atgtcagtc atcccaaca 
      atgttgttg tgtttcatc ggagttggt gtttattac 
      ttttacaaa acaaaacat aagcgtgga gggttacac 
      caccattta cgtcacggt gaagacagt attgaagct 
      ccagatcat cctatttta aagggtgtg agtttgcaa 
      catatgatt gagatgaca acgagtggt agtggctca 
      ggcttgcct ttattggtg caacgtagc attgctcgc 
      caaatccaa ttggttgaa ataatcggg caggggagg 
      tttggcgag gtgtggaga gggcgttgg aggggtgaa 
      aatgtcgct gtgaaaatt ttcagttca cgagaggaa 
      cgttcatgg tttcgtgaa gctgagatt tatcaaacc 
      gttatgtta cgacatgat aacatcctc ggttttatt 
      gcggccgat aataaagat aatgggaca tggacccaa 
      ttgtggttg attaccgat tatcacgaa tatggttcg 
      ctttttgat ttcctgggt cgcaatact ttggatacc 
      accggtatg atacgaatg gctttatct atagccaca 
      ggtcttgcc catctccat atggatatc atgggtacg 
      cagggcaag ccagcaatt gcccatcgt gacttgaag 
      tcaaagaat attttggtc aagtcgaat tttacgtgt 
      gctattggt gatttggga ttagctgtt agacatgat 
      gcgacaact gataccgtt gatattccg ctcaataat 
      cgagtgggt acgaaacgg tatatggca ccggaagtt 
      ttggacgaa acgatgaat gtaaatcat tttgattcg 
      ttcaaacga gccgatgtt tatgcgtta gggttgatt 
      ttttgggag attgcgagg cgatgtaat gtgggaggg 
      atttacgat gagtatcag ttaccgttt tatgatgct 
      gtgccgtct gatcccacc attgaagaa atgagaagg 
      gtggtgtgt gtcgataaa caaaggcct aacatacca 
      aaccgttgg cagagttgc gaagctctc cgcgtcatg 
      ttgaaacta atgaaagag tgttggtat cacaacgcg 
      acagctcgt ttaacagct ctaaggatt aaaaagaca 
      ctctctaac ttccgaaca tcggaggaa cttaaaatg 
      tgaaagttt agcatgatt gtgtcaggt aaaacgaaa 
      cgaaagcaa taattttta tttaataat taataatgt 
      tttgttaca ggtgtacaa aacaatgtg tgaacgctc 
      acgcgattt tttttttcg tttattttc cgagtatta 
      caagtgttg taagccggt cctatgtga tagagcgtt 
      gatttgtta aaaaaatgt ctcatagga tgtgatgaa 
      aggtctgta tgaaagagg agtgaaatg ttcactgcc 
      ccggtttgg ttcatatcc acaataata tacagtact 
      gcctgccca cccattcca tagtttacg agagcttct 
      tccttggtt ttgtgaccg gtaataatg tgatccttc 
      ccgattctc tgcttgatc tctatttcc gttgattgt 
      tataatctt tgatgaaat taactgccg acatgccaa 
      agccataaa cagagtcaa ttaattttt acacaaaca 
      agtaatagc tgttaaaga caatatttt ttattcaac 
      tgttgaaat caaccgtta tagcggata aagtagaga 
      tctttttat acatagtgt agatataat acgtaatag 
      ttgtacatt tgttttgtt cggtttgat tcaaaaggc 
      tcctagtca acaatagaa cagcagatt ctggcaaca 
      cctgtgtga atgtggagt gaaaatttt cctagttcc 
      tacaaatat ttggtggtt tttcgtttt gcaagcgtg 
      taaaagttg ttgcatact atttttcag ttgtaaata 
      ttttaattt tgactaagt gaggagaac gtaatttaa 
      cgtgtataa gaatttaat tgtgctaac tatattttt 
      aaggatatt tataaaaaa aataaattt actatgtaa 
      tt
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