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Gene information
General information
TC003238 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 181247..195869
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003238
    iBeetle screen iB_03524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    eyes fluorescence present - Number of animals showing the phenotype: 8

    Comment: we use crossings where only the female larvae show red fluorescence. because of the fact, that the pupa have no genital lobes, I checked the red fluorescence to be sure that they are females and no males
    female genital lobes not present - Number of animals showing the phenotype: 8

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003238 RA
      MKTEYRLCS ACGEPISDK FLLEVSGRS WHARCLRCC 
      VCQLQLDRQ PSCFIRDRA IYCKADYAK SFGAKCSVC 
      SRGISSSDW VRKARDHVY HLACFACAA CHRQLSTGE 
      EFALHEDRV LCKAHYLET LDGGTTSSD DGCDAEGYH 
      KNKAKRVRT TFTEEQLQV LQANFQLDS NPDGQDLER 
      IAQITGLSK RVTQVWFQN SRARQKKHM HTGKIKTQM 
      HNAREDNGF GRHINLHLT YSFQQQNNS KTSLFGQPE 
      SALDDLSQE STMHCMQGE V
    • >TC003238 RB
      MKTEYRLCS ACGEPISDK FLLEVSGRS WHARCLRCC 
      VCQLQLDRQ PSCFIRDRA IYCKADYAK SFGAKCSVC 
      SRGISSSDW VRKARDHVY HLACFACAA CHRQLSTGE 
      EFALHEDRV LCKAHYLET LDGGTTSSD DFTDGCDAE 
      GYHKNKAKR VRTTFTEEQ LQVLQANFQ LDSNPDGQD 
      LERIAQITG LSKRVTQVW FQNSRARQK KHMHTGKIK 
      TQMHNARED NGFGRHINL HLTYSFQQQ NNSKTSLFG 
      QPESALDDL SQESTMHCM QGEV
    CDS sequence
    • >TC003238 RA
      atgaagacg gagtatcgc ctgtgctcg gcatgcggt 
      gaaccaatt agcgacaag ttccttttg gaagtttcc 
      gggaggagt tggcacgcg agatgtctc cggtgttgt 
      gtctgtcag ctgcaattg gaccgtcaa ccctcttgt 
      ttcatccgg gaccgagca atttattgt aaggcagac 
      tatgcaaaa agtttcggc gccaaatgc tccgtatgt 
      tcccgaggc atctcctcc agcgactgg gtgcggaaa 
      gcccgtgac cacgtctac cacttggcc tgttttgca 
      tgtgctgcc tgtcatcgg cagctatcc accggtgag 
      gagtttgcc ctccatgaa gaccgcgtc ctgtgcaaa 
      gcccattac ctggagacc ctcgatgga ggcaccacc 
      tcctccgac gacggttgc gacgccgaa ggctaccac 
      aagaacaag gcgaaaagg gtgcgcacc acttttacc 
      gaagaacag ctccaagtg ctccaagcc aacttccag 
      ctggactcg aatcccgac ggccaggac ttggagcgc 
      attgctcag attacggga ctgagcaaa cgggtgaca 
      caagtgtgg tttcagaat tcgagggcg aggcaaaag 
      aaacacatg catactggg aaaattaag acacagatg 
      cataatgct agagaggat aatggattt ggacgccac 
      ataaatcta catttgacg tactctttc caacagcag 
      aacaactcg aaaacgtca ctgtttgga caacctgaa 
      tcagcttta gatgatctc tcacaggaa tcaacgatg 
      cactgtatg cagggtgaa gtgtaa
    • >TC003238 RB
      atgaagacg gagtatcgc ctgtgctcg gcatgcggt 
      gaaccaatt agcgacaag ttccttttg gaagtttcc 
      gggaggagt tggcacgcg agatgtctc cggtgttgt 
      gtctgtcag ctgcaattg gaccgtcaa ccctcttgt 
      ttcatccgg gaccgagca atttattgt aaggcagac 
      tatgcaaaa agtttcggc gccaaatgc tccgtatgt 
      tcccgaggc atctcctcc agcgactgg gtgcggaaa 
      gcccgtgac cacgtctac cacttggcc tgttttgca 
      tgtgctgcc tgtcatcgg cagctatcc accggtgag 
      gagtttgcc ctccatgaa gaccgcgtc ctgtgcaaa 
      gcccattac ctggagacc ctcgatgga ggcaccacc 
      tcctccgac gactttaca gacggttgc gacgccgaa 
      ggctaccac aagaacaag gcgaaaagg gtgcgcacc 
      acttttacc gaagaacag ctccaagtg ctccaagcc 
      aacttccag ctggactcg aatcccgac ggccaggac 
      ttggagcgc attgctcag attacggga ctgagcaaa 
      cgggtgaca caagtgtgg tttcagaat tcgagggcg 
      aggcaaaag aaacacatg catactggg aaaattaag 
      acacagatg cataatgct agagaggat aatggattt 
      ggacgccac ataaatcta catttgacg tactctttc 
      caacagcag aacaactcg aaaacgtca ctgtttgga 
      caacctgaa tcagcttta gatgatctc tcacaggaa 
      tcaacgatg cactgtatg cagggtgaa gtgtaa
    mRNA sequence
    • >TC003238 RA
      tcttgttga attgaaaat tttttgtgg agatttggg 
      gtcgggcta acagtgtgg tggtgaaat gggattttt 
      cagacattt gcaaatttt tcgtgcaat attttgaga 
      cttgtgtgc gcgctggag gcctgaaaa acattcggg 
      aaaggagat tttttgaaa atgaagacg gagtatcgc 
      ctgtgctcg gcatgcggt gaaccaatt agcgacaag 
      ttccttttg gaagtttcc gggaggagt tggcacgcg 
      agatgtctc cggtgttgt gtctgtcag ctgcaattg 
      gaccgtcaa ccctcttgt ttcatccgg gaccgagca 
      atttattgt aaggcagac tatgcaaaa agtttcggc 
      gccaaatgc tccgtatgt tcccgaggc atctcctcc 
      agcgactgg gtgcggaaa gcccgtgac cacgtctac 
      cacttggcc tgttttgca tgtgctgcc tgtcatcgg 
      cagctatcc accggtgag gagtttgcc ctccatgaa 
      gaccgcgtc ctgtgcaaa gcccattac ctggagacc 
      ctcgatgga ggcaccacc tcctccgac gacggttgc 
      gacgccgaa ggctaccac aagaacaag gcgaaaagg 
      gtgcgcacc acttttacc gaagaacag ctccaagtg 
      ctccaagcc aacttccag ctggactcg aatcccgac 
      ggccaggac ttggagcgc attgctcag attacggga 
      ctgagcaaa cgggtgaca caagtgtgg tttcagaat 
      tcgagggcg aggcaaaag aaacacatg catactggg 
      aaaattaag acacagatg cataatgct agagaggat 
      aatggattt ggacgccac ataaatcta catttgacg 
      tactctttc caacagcag aacaactcg aaaacgtca 
      ctgtttgga caacctgaa tcagcttta gatgatctc 
      tcacaggaa tcaacgatg cactgtatg cagggtgaa 
      gtgtaaaca gctttatta tttggaaat acttgtatt 
      ggagcgccc caatatact acgtgattt ttt
    • >TC003238 RB
      tcttgttga attgaaaat tttttgtgg agatttggg 
      gtcgggcta acagtgtgg tggtgaaat gggattttt 
      cagacattt gcaaatttt tcgtgcaat attttgaga 
      cttgtgtgc gcgctggag gcctgaaaa acattcggg 
      aaaggagat tttttgaaa atgaagacg gagtatcgc 
      ctgtgctcg gcatgcggt gaaccaatt agcgacaag 
      ttccttttg gaagtttcc gggaggagt tggcacgcg 
      agatgtctc cggtgttgt gtctgtcag ctgcaattg 
      gaccgtcaa ccctcttgt ttcatccgg gaccgagca 
      atttattgt aaggcagac tatgcaaaa agtttcggc 
      gccaaatgc tccgtatgt tcccgaggc atctcctcc 
      agcgactgg gtgcggaaa gcccgtgac cacgtctac 
      cacttggcc tgttttgca tgtgctgcc tgtcatcgg 
      cagctatcc accggtgag gagtttgcc ctccatgaa 
      gaccgcgtc ctgtgcaaa gcccattac ctggagacc 
      ctcgatgga ggcaccacc tcctccgac gactttaca 
      gacggttgc gacgccgaa ggctaccac aagaacaag 
      gcgaaaagg gtgcgcacc acttttacc gaagaacag 
      ctccaagtg ctccaagcc aacttccag ctggactcg 
      aatcccgac ggccaggac ttggagcgc attgctcag 
      attacggga ctgagcaaa cgggtgaca caagtgtgg 
      tttcagaat tcgagggcg aggcaaaag aaacacatg 
      catactggg aaaattaag acacagatg cataatgct 
      agagaggat aatggattt ggacgccac ataaatcta 
      catttgacg tactctttc caacagcag aacaactcg 
      aaaacgtca ctgtttgga caacctgaa tcagcttta 
      gatgatctc tcacaggaa tcaacgatg cactgtatg 
      cagggtgaa gtgtaaaca gctttatta tttggaaat 
      acttgtatt ggagcgccc caatatact acgtgattt 
      ttt
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