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Gene information
General information
TC003228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 108019..112997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003228
    iBeetle screen iB_03523

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003228 RA
      MAEKTGVST NNLVEAGDY GKSKKLPQY IAALSVCMG 
      SVAAGTVLG WTGNITKEN LANRTLNDI YVDPDNDYG 
      WIGSFSTLG ALCMCFPIG FICDLIGRK LAMLLTIIP 
      FSVGWLLII FADSTAMIF AGRFLTGLA GGAFCVSAP 
      MYTSEIAEK DIRGALGSY FQLLLTVGI LFAYLLGAF 
      LKPQIVSII CACVPLVFG VVFFLQPET PVYSLKKGN 
      EEAAIKALR KLRGDEYNV EAEIADIKA NIEKDEREK 
      IPLSKSLET RAAKISLLI CFGLMFFQQ LGGINAVIF 
      YVGTIFEEA DSGLSASDV TILVGVMQV IATFVSSLV 
      IDKFGRKIL LLISGFIMS IAGILIGIY FSLKDDVSD 
      IGFLPILGV CIFIIVFSL GFGPIPWMI SSEVFPAEI 
      KSNASSAAG TFNWFLAFL VTKFYGDLA AEIGKDVTF 
      YIFAGISLV GVVFIFFVI PETKGKTLD EIQRELNGE 
      KNVGQGIDN QGFNS
    CDS sequence
    • >TC003228 RA
      atggctgaa aaaactggt gttagcact aataatcta 
      gtagaagct ggtgattat ggcaagtct aagaagttg 
      ccccaatac attgctgct ctctcagta tgtatgggc 
      tcagtggcg gccggcacc gtcttaggc tggacgggc 
      aacatcacc aaagagaac ctcgctaat cgcaccctt 
      aacgacatc tacgtggac cctgacaac gactacggt 
      tggatcggt tctttcagc acccttgga gctctctgc 
      atgtgtttt cccatcggt ttcatctgc gacttaatc 
      ggccgtaaa ctcgctatg ctcctcacc atcatcccc 
      ttcagcgtt ggttggctt ctcattatt ttcgccgac 
      tccacagcg atgattttc gctggaaga ttcctcaca 
      ggtctcgct ggaggtgcg ttctgcgtc tccgccccc 
      atgtacacc agcgaaatc gccgagaaa gatatcaga 
      ggggcacta ggaagctac ttccaactc ctcctcacc 
      gttggaatt ctctttgcc taccttttg ggtgcattt 
      ctcaaaccg caaattgtt tcgattatt tgcgcttgc 
      gttccgttg gttttcggt gttgtgttt ttccttcaa 
      cccgaaact cccgtctac agtttgaag aaagggaac 
      gaagaagcg gcgattaaa gctttgagg aaactgcga 
      ggtgatgaa tataatgtt gaagcagaa attgctgat 
      attaaagcg aatattgaa aaagacgaa agggaaaaa 
      atccctttg tctaagtct ttggaaaca agagctgcg 
      aaaatatcg ctacttatt tgcttcggt ttaatgttt 
      ttccaacag ttgggaggc atcaatgct gttattttc 
      tatgtagga acaattttc gaagaagct gattcaggt 
      ttgtctgct tccgatgtt acaatcctc gttggtgtg 
      atgcaagtc attgcaact tttgtctcg tcccttgtt 
      atcgataaa ttcggacgg aaaattctt ctccttatt 
      tctggtttt atcatgtca attgctgga attcttatt 
      ggaatttat ttctcacta aaagacgac gttagtgat 
      attggtttt ctcccaatt ttgggagtt tgcattttc 
      attattgtt ttttcgtta ggttttgga ccaattcct 
      tggatgatt tcgtcggaa gtttttcca gccgaaatc 
      aaaagtaat gccagttcg gctgcggga actttcaat 
      tggtttttg gcttttctt gtaaccaaa ttttacggc 
      gatttggca gccgagatt ggaaaagac gttacgttt 
      tatattttt gcgggtatt tctttggtc ggagttgtt 
      tttattttc tttgtcatt ccggaaacg aaagggaag 
      actttggat gaaattcag agggaactc aatggagag 
      aaaaatgtg gggcaagga attgacaat caagggttt 
      aatagttag
    mRNA sequence
    • >TC003228 RA
      ttatggcgg gagaacgat ctgcacgca taaagcttt 
      tcatacatg gacaggact gttagaaat tatgtgaac 
      agatcgtga ccaaggagg aagtgttga gtggctttt 
      aagtgtgat tttgtgcaa aatggctga aaaaactgg 
      tgttagcac taataatct agtagaagc tggtgatta 
      tggcaagtc taagaagtt gccccaata cattgctgc 
      tctctcagt atgtatggg ctcagtggc ggccggcac 
      cgtcttagg ctggacggg caacatcac caaagagaa 
      cctcgctaa tcgcaccct taacgacat ctacgtgga 
      ccctgacaa cgactacgg ttggatcgg ttctttcag 
      cacccttgg agctctctg catgtgttt tcccatcgg 
      tttcatctg cgacttaat cggccgtaa actcgctat 
      gctcctcac catcatccc cttcagcgt tggttggct 
      tctcattat tttcgccga ctccacagc gatgatttt 
      cgctggaag attcctcac aggtctcgc tggaggtgc 
      gttctgcgt ctccgcccc catgtacac cagcgaaat 
      cgccgagaa agatatcag aggggcact aggaagcta 
      cttccaact cctcctcac cgttggaat tctctttgc 
      ctacctttt gggtgcatt tctcaaacc gcaaattgt 
      ttcgattat ttgcgcttg cgttccgtt ggttttcgg 
      tgttgtgtt tttccttca acccgaaac tcccgtcta 
      cagtttgaa gaaagggaa cgaagaagc ggcgattaa 
      agctttgag gaaactgcg aggtgatga atataatgt 
      tgaagcaga aattgctga tattaaagc gaatattga 
      aaaagacga aagggaaaa aatcccttt gtctaagtc 
      tttggaaac aagagctgc gaaaatatc gctacttat 
      ttgcttcgg tttaatgtt tttccaaca gttgggagg 
      catcaatgc tgttatttt ctatgtagg aacaatttt 
      cgaagaagc tgattcagg tttgtctgc ttccgatgt 
      tacaatcct cgttggtgt gatgcaagt cattgcaac 
      ttttgtctc gtcccttgt tatcgataa attcggacg 
      gaaaattct tctccttat ttctggttt tatcatgtc 
      aattgctgg aattcttat tggaattta tttctcact 
      aaaagacga cgttagtga tattggttt tctcccaat 
      tttgggagt ttgcatttt cattattgt tttttcgtt 
      aggttttgg accaattcc ttggatgat ttcgtcgga 
      agtttttcc agccgaaat caaaagtaa tgccagttc 
      ggctgcggg aactttcaa ttggttttt ggcttttct 
      tgtaaccaa attttacgg cgatttggc agccgagat 
      tggaaaaga cgttacgtt ttatatttt tgcgggtat 
      ttctttggt cggagttgt ttttatttt ctttgtcat 
      tccggaaac gaaagggaa gactttgga tgaaattca 
      gagggaact caatggaga gaaaaatgt ggggcaagg 
      aattgacaa tcaagggtt taatagtta gttttagtc 
      attagtgaa taaaagtat tatttataa agtacttaa 
      ctcaatact tgtattgtt atttataaa tatcatatg 
      acataaaac agaaagaag tgcccttaa acgcacttt 
      tcttacctg atatttagt aatgaccaa ctccatact 
      gtacttttc tagtcaagt tctattgta ttgaaaata 
      tcacaagca ataattatt gtacgagta ctgtgcaaa 
      actttataa aattaaacg atatttaaa cattaacag 
      tgttttctt catgctgtt tgggaaatt tattaagtt 
      aaaaaaatg ccaaaaaat taaaattag tccacacag 
      aaagcaata aataaaaaa gaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY