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Gene information
General information
TC003221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 41056..52075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC003221
      iBeetle screen iB_03520

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection

      remarks: later than schedule,
      Adult morphology
      wings not closed - Number of animals showing the phenotype: 2

      remarks: later than schedule,
      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003221 RA
        MWPRTVLMD YIFLLAFIG FCGGAIMGD ECDWTGSGL 
SSSATGRGV TPVYLRCSA GRVIWLYPR GALRVLLRL 
PGQDREFRA CIKVHPDEK KEPAARLFL EGPRSLLPL 
YADPANKAI RCFISKNGQ AAIYVEASD VDAITKRVA 
EIEYDLQAL PKGVKGFDP TEECRPCTN EELAHAFCT 
SDLVTRGVI HGVENHEDL EISQMSVKV TKLIRHTVD 
DAFTVDTNS ISPEEDTPL EKITLHVAQ HCGVSHGLG 
EFVFMARRK LGDLTLQCA PRLEDWALL VNQLSEEGK 
AHCVLRS
      CDS sequence
      • >TC003221 RA
        atgtggcct cggaccgtt cttatggat tatattttt 
ctcctcgct ttcatcgga ttttgcgga ggcgctatc 
atgggagac gagtgcgac tggaccggg agcggattg 
agctcgtca gcgacgggc agaggagtc actcctgtc 
tacctgcgc tgttcggct ggaagagtg atctggttg 
tacccaaga ggggcactc agagtcctc cttcgtctc 
ccgggacaa gaccgcgaa ttccgggcc tgcatcaaa 
gtccatccc gacgaaaaa aaagaaccg gcggccagg 
ctcttcctc gaagggccc agatctctt ctaccactc 
tacgcggac ccagccaat aaagccatc agatgcttc 
ataagtaag aacggccaa gcggccatc tacgtggag 
gcctctgac gtggatgcc atcaccaaa agggtggca 
gaaatcgaa tatgatctc caagcgttg cccaaaggc 
gtcaaagga ttcgacccc acagaagaa tgtcgaccg 
tgtactaac gaggaattg gcgcacgca ttctgcacc 
agtgatctc gtaactcga ggtgtcatc cacggcgtg 
gagaaccac gaggacctg gagatctct caaatgtcc 
gtcaaggtg acaaaactc atccgccac accgtcgac 
gacgccttc acagtcgac accaactct atctctcca 
gaggaggac acccctctc gaaaaaatt acacttcac 
gtggcccaa cactgtgga gtctcgcac ggtttgggc 
gaattcgtg tttatggcg cgccgaaaa ctcggcgat 
ttgactcta caatgcgcc cccagatta gaagactgg 
gctcttttg gtcaaccaa ttgtccgaa gagggcaaa 
gcccactgt gtcctgcga agttag
      mRNA sequence
      • >TC003221 RA
        cccctaatg taaagctcc aacaagtgg gaggagcga 
tcggccatc tccggccat cgccgccgt tcaaatcac 
ttgttggtt gaaacaaat tcaaaagtc agtcgctct 
cgacggttt ccgcggatg gttggtgcc aagttacag 
cgatttgtt tacgatctt tctctctcc aaatgggtg 
aactgtgat gtggatgat tttcgtgat gtggcctcg 
gaccgttct tatggatta tatttttct cctcgcttt 
catcggatt ttgcggagg cgctatcat gggagacga 
gtgcgactg gaccgggag cggattgag ctcgtcagc 
gacgggcag aggagtcac tcctgtcta cctgcgctg 
ttcggctgg aagagtgat ctggttgta cccaagagg 
ggcactcag agtcctcct tcgtctccc gggacaaga 
ccgcgaatt ccgggcctg catcaaagt ccatcccga 
cgaaaaaaa agaaccggc ggccaggct cttcctcga 
agggcccag atctcttct accactcta cgcggaccc 
agccaataa agccatcag atgcttcat aagtaagaa 
cggccaagc ggccatcta cgtggaggc ctctgacgt 
ggatgccat caccaaaag ggtggcaga aatcgaata 
tgatctcca agcgttgcc caaaggcgt caaaggatt 
cgaccccac agaagaatg tcgaccgtg tactaacga 
ggaattggc gcacgcatt ctgcaccag tgatctcgt 
aactcgagg tgtcatcca cggcgtgga gaaccacga 
ggacctgga gatctctca aatgtccgt caaggtgac 
aaaactcat ccgccacac cgtcgacga cgccttcac 
agtcgacac caactctat ctctccaga ggaggacac 
ccctctcga aaaaattac acttcacgt ggcccaaca 
ctgtggagt ctcgcacgg tttgggcga attcgtgtt 
tatggcgcg ccgaaaact cggcgattt gactctaca 
atgcgcccc cagattaga agactgggc tcttttggt 
caaccaatt gtccgaaga gggcaaagc ccactgtgt 
cctgcgaag ttagcccca ccacaaagt aacttaagt 
gcgctgctg tgatttgtt tgtatgtac ataggaaag 
acttgaatg cgacggtgc ttagcgaaa aatcgagcc 
agattggga ctagtgtgc aatgtgcaa tttctctgg 
ttttagtgt acatagatg tgctccaca ttggagctt 
tacggtctc tttttttat aaaacgagg ttccattat 
tgaattctt ggtgccaaa atagagccg cttaaaact 
aatctactt tctgttgta tgtacttgt aattactaa 
ttactttta acttttttc tttcttaat gtgtacata 
aagtattca ttggatctc acattagat tgtgttacg 
aatgcgaac aaatgaaat cttattatt tattgttcc 
tagtactgt aaaatcgtg ataagattt cgtttcgtt 
gcactttta ttattatag tttttaatc gtctgttgc 
ttaagaaca cgaatgcca taatcgtct tctcactag 
acagagaac gctgtgata tgtaaaaaa ctatatttt 
tattgaatt gtattgttt gcaaagaaa tatatgaat 
tttaaattt taatacgat ttcattttc attccagtt 
caataagta aaaaaagtt tgaaattgt gaccgccgt 
ttttatttg taatatgac gcaatatag aaaaaaagc 
actgtaaaa tctccttcc ttgtgtatt tcccgcttg 
tttgttgtt tattggaca aaaagcaac ttcctcgtg 
ccccgattg agatcatcc aaacaattg gttaaattg 
ctacaaatt atgcatttt tatcgccgc cttgcacac 
ttgtgatgc ttaactttt tccacttcc tctggcgct 
tccgaatgc gtgacttga aatcgtgtg aatgaatac 
acaattgtt ttattacca catgtgtca ataaatctt 
gttttgaaa g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY