General information | |||
TC003221 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 41056..52075 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC003221 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03520 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
remarks: later than schedule,
- >TC003221 RA
MWPRTVLMD YIFLLAFIG FCGGAIMGD ECDWTGSGL SSSATGRGV TPVYLRCSA GRVIWLYPR GALRVLLRL PGQDREFRA CIKVHPDEK KEPAARLFL EGPRSLLPL YADPANKAI RCFISKNGQ AAIYVEASD VDAITKRVA EIEYDLQAL PKGVKGFDP TEECRPCTN EELAHAFCT SDLVTRGVI HGVENHEDL EISQMSVKV TKLIRHTVD DAFTVDTNS ISPEEDTPL EKITLHVAQ HCGVSHGLG EFVFMARRK LGDLTLQCA PRLEDWALL VNQLSEEGK AHCVLRS
- >TC003221 RA
atgtggcct cggaccgtt cttatggat tatattttt ctcctcgct ttcatcgga ttttgcgga ggcgctatc atgggagac gagtgcgac tggaccggg agcggattg agctcgtca gcgacgggc agaggagtc actcctgtc tacctgcgc tgttcggct ggaagagtg atctggttg tacccaaga ggggcactc agagtcctc cttcgtctc ccgggacaa gaccgcgaa ttccgggcc tgcatcaaa gtccatccc gacgaaaaa aaagaaccg gcggccagg ctcttcctc gaagggccc agatctctt ctaccactc tacgcggac ccagccaat aaagccatc agatgcttc ataagtaag aacggccaa gcggccatc tacgtggag gcctctgac gtggatgcc atcaccaaa agggtggca gaaatcgaa tatgatctc caagcgttg cccaaaggc gtcaaagga ttcgacccc acagaagaa tgtcgaccg tgtactaac gaggaattg gcgcacgca ttctgcacc agtgatctc gtaactcga ggtgtcatc cacggcgtg gagaaccac gaggacctg gagatctct caaatgtcc gtcaaggtg acaaaactc atccgccac accgtcgac gacgccttc acagtcgac accaactct atctctcca gaggaggac acccctctc gaaaaaatt acacttcac gtggcccaa cactgtgga gtctcgcac ggtttgggc gaattcgtg tttatggcg cgccgaaaa ctcggcgat ttgactcta caatgcgcc cccagatta gaagactgg gctcttttg gtcaaccaa ttgtccgaa gagggcaaa gcccactgt gtcctgcga agttag
- >TC003221 RA
cccctaatg taaagctcc aacaagtgg gaggagcga tcggccatc tccggccat cgccgccgt tcaaatcac ttgttggtt gaaacaaat tcaaaagtc agtcgctct cgacggttt ccgcggatg gttggtgcc aagttacag cgatttgtt tacgatctt tctctctcc aaatgggtg aactgtgat gtggatgat tttcgtgat gtggcctcg gaccgttct tatggatta tatttttct cctcgcttt catcggatt ttgcggagg cgctatcat gggagacga gtgcgactg gaccgggag cggattgag ctcgtcagc gacgggcag aggagtcac tcctgtcta cctgcgctg ttcggctgg aagagtgat ctggttgta cccaagagg ggcactcag agtcctcct tcgtctccc gggacaaga ccgcgaatt ccgggcctg catcaaagt ccatcccga cgaaaaaaa agaaccggc ggccaggct cttcctcga agggcccag atctcttct accactcta cgcggaccc agccaataa agccatcag atgcttcat aagtaagaa cggccaagc ggccatcta cgtggaggc ctctgacgt ggatgccat caccaaaag ggtggcaga aatcgaata tgatctcca agcgttgcc caaaggcgt caaaggatt cgaccccac agaagaatg tcgaccgtg tactaacga ggaattggc gcacgcatt ctgcaccag tgatctcgt aactcgagg tgtcatcca cggcgtgga gaaccacga ggacctgga gatctctca aatgtccgt caaggtgac aaaactcat ccgccacac cgtcgacga cgccttcac agtcgacac caactctat ctctccaga ggaggacac ccctctcga aaaaattac acttcacgt ggcccaaca ctgtggagt ctcgcacgg tttgggcga attcgtgtt tatggcgcg ccgaaaact cggcgattt gactctaca atgcgcccc cagattaga agactgggc tcttttggt caaccaatt gtccgaaga gggcaaagc ccactgtgt cctgcgaag ttagcccca ccacaaagt aacttaagt gcgctgctg tgatttgtt tgtatgtac ataggaaag acttgaatg cgacggtgc ttagcgaaa aatcgagcc agattggga ctagtgtgc aatgtgcaa tttctctgg ttttagtgt acatagatg tgctccaca ttggagctt tacggtctc tttttttat aaaacgagg ttccattat tgaattctt ggtgccaaa atagagccg cttaaaact aatctactt tctgttgta tgtacttgt aattactaa ttactttta acttttttc tttcttaat gtgtacata aagtattca ttggatctc acattagat tgtgttacg aatgcgaac aaatgaaat cttattatt tattgttcc tagtactgt aaaatcgtg ataagattt cgtttcgtt gcactttta ttattatag tttttaatc gtctgttgc ttaagaaca cgaatgcca taatcgtct tctcactag acagagaac gctgtgata tgtaaaaaa ctatatttt tattgaatt gtattgttt gcaaagaaa tatatgaat tttaaattt taatacgat ttcattttc attccagtt caataagta aaaaaagtt tgaaattgt gaccgccgt ttttatttg taatatgac gcaatatag aaaaaaagc actgtaaaa tctccttcc ttgtgtatt tcccgcttg tttgttgtt tattggaca aaaagcaac ttcctcgtg ccccgattg agatcatcc aaacaattg gttaaattg ctacaaatt atgcatttt tatcgccgc cttgcacac ttgtgatgc ttaactttt tccacttcc tctggcgct tccgaatgc gtgacttga aatcgtgtg aatgaatac acaattgtt ttattacca catgtgtca ataaatctt gttttgaaa g
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
