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Gene information
General information
TC003221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 41056..52075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003221
      iBeetle screen iB_03520
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection



      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      remarks: later than schedule,
      Adult morphology
      wings not closed - Number of animals showing the phenotype: 2

      remarks: later than schedule,
      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003221 RA
        MWPRTVLMD YIFLLAFIG FCGGAIMGD ECDWTGSGL 
        SSSATGRGV TPVYLRCSA GRVIWLYPR GALRVLLRL 
        PGQDREFRA CIKVHPDEK KEPAARLFL EGPRSLLPL 
        YADPANKAI RCFISKNGQ AAIYVEASD VDAITKRVA 
        EIEYDLQAL PKGVKGFDP TEECRPCTN EELAHAFCT 
        SDLVTRGVI HGVENHEDL EISQMSVKV TKLIRHTVD 
        DAFTVDTNS ISPEEDTPL EKITLHVAQ HCGVSHGLG 
        EFVFMARRK LGDLTLQCA PRLEDWALL VNQLSEEGK 
        AHCVLRS
      CDS sequence
      • >TC003221 RA
        atgtggcct cggaccgtt cttatggat tatattttt 
        ctcctcgct ttcatcgga ttttgcgga ggcgctatc 
        atgggagac gagtgcgac tggaccggg agcggattg 
        agctcgtca gcgacgggc agaggagtc actcctgtc 
        tacctgcgc tgttcggct ggaagagtg atctggttg 
        tacccaaga ggggcactc agagtcctc cttcgtctc 
        ccgggacaa gaccgcgaa ttccgggcc tgcatcaaa 
        gtccatccc gacgaaaaa aaagaaccg gcggccagg 
        ctcttcctc gaagggccc agatctctt ctaccactc 
        tacgcggac ccagccaat aaagccatc agatgcttc 
        ataagtaag aacggccaa gcggccatc tacgtggag 
        gcctctgac gtggatgcc atcaccaaa agggtggca 
        gaaatcgaa tatgatctc caagcgttg cccaaaggc 
        gtcaaagga ttcgacccc acagaagaa tgtcgaccg 
        tgtactaac gaggaattg gcgcacgca ttctgcacc 
        agtgatctc gtaactcga ggtgtcatc cacggcgtg 
        gagaaccac gaggacctg gagatctct caaatgtcc 
        gtcaaggtg acaaaactc atccgccac accgtcgac 
        gacgccttc acagtcgac accaactct atctctcca 
        gaggaggac acccctctc gaaaaaatt acacttcac 
        gtggcccaa cactgtgga gtctcgcac ggtttgggc 
        gaattcgtg tttatggcg cgccgaaaa ctcggcgat 
        ttgactcta caatgcgcc cccagatta gaagactgg 
        gctcttttg gtcaaccaa ttgtccgaa gagggcaaa 
        gcccactgt gtcctgcga agttag
      mRNA sequence
      • >TC003221 RA
        cccctaatg taaagctcc aacaagtgg gaggagcga 
        tcggccatc tccggccat cgccgccgt tcaaatcac 
        ttgttggtt gaaacaaat tcaaaagtc agtcgctct 
        cgacggttt ccgcggatg gttggtgcc aagttacag 
        cgatttgtt tacgatctt tctctctcc aaatgggtg 
        aactgtgat gtggatgat tttcgtgat gtggcctcg 
        gaccgttct tatggatta tatttttct cctcgcttt 
        catcggatt ttgcggagg cgctatcat gggagacga 
        gtgcgactg gaccgggag cggattgag ctcgtcagc 
        gacgggcag aggagtcac tcctgtcta cctgcgctg 
        ttcggctgg aagagtgat ctggttgta cccaagagg 
        ggcactcag agtcctcct tcgtctccc gggacaaga 
        ccgcgaatt ccgggcctg catcaaagt ccatcccga 
        cgaaaaaaa agaaccggc ggccaggct cttcctcga 
        agggcccag atctcttct accactcta cgcggaccc 
        agccaataa agccatcag atgcttcat aagtaagaa 
        cggccaagc ggccatcta cgtggaggc ctctgacgt 
        ggatgccat caccaaaag ggtggcaga aatcgaata 
        tgatctcca agcgttgcc caaaggcgt caaaggatt 
        cgaccccac agaagaatg tcgaccgtg tactaacga 
        ggaattggc gcacgcatt ctgcaccag tgatctcgt 
        aactcgagg tgtcatcca cggcgtgga gaaccacga 
        ggacctgga gatctctca aatgtccgt caaggtgac 
        aaaactcat ccgccacac cgtcgacga cgccttcac 
        agtcgacac caactctat ctctccaga ggaggacac 
        ccctctcga aaaaattac acttcacgt ggcccaaca 
        ctgtggagt ctcgcacgg tttgggcga attcgtgtt 
        tatggcgcg ccgaaaact cggcgattt gactctaca 
        atgcgcccc cagattaga agactgggc tcttttggt 
        caaccaatt gtccgaaga gggcaaagc ccactgtgt 
        cctgcgaag ttagcccca ccacaaagt aacttaagt 
        gcgctgctg tgatttgtt tgtatgtac ataggaaag 
        acttgaatg cgacggtgc ttagcgaaa aatcgagcc 
        agattggga ctagtgtgc aatgtgcaa tttctctgg 
        ttttagtgt acatagatg tgctccaca ttggagctt 
        tacggtctc tttttttat aaaacgagg ttccattat 
        tgaattctt ggtgccaaa atagagccg cttaaaact 
        aatctactt tctgttgta tgtacttgt aattactaa 
        ttactttta acttttttc tttcttaat gtgtacata 
        aagtattca ttggatctc acattagat tgtgttacg 
        aatgcgaac aaatgaaat cttattatt tattgttcc 
        tagtactgt aaaatcgtg ataagattt cgtttcgtt 
        gcactttta ttattatag tttttaatc gtctgttgc 
        ttaagaaca cgaatgcca taatcgtct tctcactag 
        acagagaac gctgtgata tgtaaaaaa ctatatttt 
        tattgaatt gtattgttt gcaaagaaa tatatgaat 
        tttaaattt taatacgat ttcattttc attccagtt 
        caataagta aaaaaagtt tgaaattgt gaccgccgt 
        ttttatttg taatatgac gcaatatag aaaaaaagc 
        actgtaaaa tctccttcc ttgtgtatt tcccgcttg 
        tttgttgtt tattggaca aaaagcaac ttcctcgtg 
        ccccgattg agatcatcc aaacaattg gttaaattg 
        ctacaaatt atgcatttt tatcgccgc cttgcacac 
        ttgtgatgc ttaactttt tccacttcc tctggcgct 
        tccgaatgc gtgacttga aatcgtgtg aatgaatac 
        acaattgtt ttattacca catgtgtca ataaatctt 
        gttttgaaa g