Status
Please wait ...
Gene information
General information
TC003205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 258594..259587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003205
    iBeetle screen iB_03519

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003205 RA
      MNLSRFRLI TFDVTDTLL KFRSAPGKQ YGEVGAMYG 
      VLVDSNSLS ANFKSHWHK MNAEHPNFG KNGLGWQSW 
      WKQIVVGTF KDSKLDLDD RKLDSIASH LIELYETSM 
      CWQPSYGAL GLLSYLRHR GVPMGVISN FDPRLDSTL 
      VNTKLRHYF KFVTASYEV GVAKPSQGI FEKAMEMSG 
      ISDIKPEEC LHVGNTVLL DYVGARKSG WSAALIHDK 
      DLKQVKEKY PFVNGNYVF SSLYELHRY FLENSDVKL 
      SAQSF
    CDS sequence
    • >TC003205 RA
      atgaacctc tcccgtttc cggttaata accttcgac 
      gtgaccgac accctcctc aaattccgc tcggctccg 
      ggcaagcaa tacggcgaa gtgggcgcc atgtacggc 
      gtcctcgtc gactccaac tcactcagc gccaatttc 
      aaatcacac tggcataaa atgaacgca gaacacccc 
      aatttcggc aaaaatggc cttggctgg caaagctgg 
      tggaagcaa attgtggtg ggcaccttc aaggactct 
      aaattggac ctggacgac cggaaactg gactcaatc 
      gcttcgcat ttaatcgaa ttgtatgaa acctccatg 
      tgctggcag cccagttat ggggctttg gggctgttg 
      tcgtatttg agacaccgg ggggtgccc atgggggtg 
      atctctaat tttgaccca agactggac tcgactttg 
      gtcaatacg aagttgagg cactatttt aagtttgtg 
      acagcgtct tatgaagtc ggagtggct aaacctagt 
      caggggatt ttcgaaaaa gcaatggaa atgtctggg 
      attagcgat atcaagcct gaggagtgt ctccatgtt 
      gggaacacc gttcttttg gattatgtt ggggccagg 
      aagagtgga tggagtgct gcgttgata catgacaaa 
      gacttgaaa caagttaaa gagaaatat cccttcgtt 
      aatggcaat tacgtgttt tcgagtttg tacgaactg 
      catagatat tttttagaa aatagtgac gttaaatta 
      agtgcgcaa tcgttttga
    mRNA sequence
    • >TC003205 RA
      tatcagttc gatgtagcc gtgagtgtc aacctcagc 
      aggactgaa ttcctgcca ttcagtcag tgcaaactc 
      ctcgtaagt gccacaatg aacctctcc cgtttccgg 
      ttaataacc ttcgacgtg accgacacc ctcctcaaa 
      ttccgctcg gctccgggc aagcaatac ggcgaagtg 
      ggcgccatg tacggcgtc ctcgtcgac tccaactca 
      ctcagcgcc aatttcaaa tcacactgg cataaaatg 
      aacgcagaa caccccaat ttcggcaaa aatggcctt 
      ggctggcaa agctggtgg aagcaaatt gtggtgggc 
      accttcaag gactctaaa ttggacctg gacgaccgg 
      aaactggac tcaatcgct tcgcattta atcgaattg 
      tatgaaacc tccatgtgc tggcagccc agttatggg 
      gctttgggg ctgttgtcg tatttgaga caccggggg 
      gtgcccatg ggggtgatc tctaatttt gacccaaga 
      ctggactcg actttggtc aatacgaag ttgaggcac 
      tattttaag tttgtgaca gcgtcttat gaagtcgga 
      gtggctaaa cctagtcag gggattttc gaaaaagca 
      atggaaatg tctgggatt agcgatatc aagcctgag 
      gagtgtctc catgttggg aacaccgtt cttttggat 
      tatgttggg gccaggaag agtggatgg agtgctgcg 
      ttgatacat gacaaagac ttgaaacaa gttaaagag 
      aaatatccc ttcgttaat ggcaattac gtgttttcg 
      agtttgtac gaactgcat agatatttt ttagaaaat 
      agtgacgtt aaattaagt gcgcaatcg ttttgaata 
      agaccaaag tattgtttt gttgtattt attttattt 
      tattttgga tcatgtctc ggaagacga atctttatc 
      ttatcgcag tggaatgaa tatgcgatt ctgtgaaac 
      aaaataaac tgttttcaa atct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY