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Gene information
General information
TC003194 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 401237..405688
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003194
    iBeetle screen iB_03517

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003194 RA
      MSLLLQLKA AQLEWNHNW QILNEKKPT MDFESPDQT 
      HPSRHQRLL KKHVLDLEK TSKLLHREN ERLKTRFLK 
      VEEELDLLQ KYVENKDER INGNEIQDY INRERVQEV 
      GRDEFGRRL LKNSESIAN LTKQMSNFD KLHLSMLEL 
      LENVESIEN KVDVGLPDF RKEISKLEI QIGDAVSQI 
      SLLREDQTN TRQSVKAIG VSVSNLQDK ADTERKRVR 
      KVEEQLESL TKAASMQTA KLHDHILKT ESSTLDTNQ 
      TKSTIHLVQ ELKSFENEY KSIVNKLPH DCGAVSGPS 
      GVYLISPGD GEPILAYCT NGWTTIQKR YDGSVDFNR 
      NWNDYSNGF GSATGEHWL GNRNLHYLT QKNCTRLQI 
      NMKDIYGKY WQAEYEEFN VGDYSMGFK LVVGGYSGN 
      ASDALDYQN HMEFSTIDN DRDISNTHC ASNYEGGWW 
      FSHCQHANL NGRYNLGLT WFDSSRNEW IAVSKSEMK 
      VKQRENC
    CDS sequence
    • >TC003194 RA
      atgtctcta ttgttgcag ttgaaagcc gcccaattg 
      gaatggaat cacaattgg caaattttg aacgagaaa 
      aaaccaaca atggacttt gaatctccg gaccaaacc 
      cacccatca cgccatcaa agactcctc aaaaaacac 
      gtcctcgac ctggagaag acgtcaaaa ctcctccat 
      cgcgagaat gaacgtctg aaaacgcgc ttcttgaaa 
      gtggaggag gaattagac ctattgcag aaatacgtc 
      gagaataag gacgaaagg atcaacggc aacgaaatc 
      caggactac atcaaccgg gagcgcgtc caggaagtc 
      ggccgggat gagttcggc cgtcgcttg ttgaaaaac 
      agcgaaagc atcgccaat ttgactaaa caaatgtcg 
      aatttcgat aaattacac ttgtccatg ttggagttg 
      ttggaaaac gtcgaaagt atcgagaat aaggtcgat 
      gtgggtctg cctgacttc cgcaaagag atctcgaaa 
      ttggaaatt caaatcggc gatgccgtt tcgcagatt 
      tctttgtta agggaagat caaacaaat acgagacag 
      tcggttaaa gccatcggg gtgagtgtg tccaatttg 
      caagataaa gccgacacg gagcgaaaa cgagtgcgg 
      aaagtcgag gagcagctt gagagtttg accaaagcg 
      gcttccatg cagacggcc aagttgcac gatcatatt 
      ttgaagacc gagtcgtcg acactcgac accaaccag 
      accaagtcc accatccac ctggtgcag gaactgaaa 
      tcgttcgaa aacgagtac aagagcatc gtcaataag 
      ttaccacac gattgtggc gcggtttct ggacctagt 
      ggagtctat ttgatttca ccaggagat ggtgaaccg 
      atcctggct tattgcacc aacggctgg accaccatc 
      cagaaacgc tacgacggt tcggtggat ttcaaccgc 
      aactggaac gactactcc aacgggttc ggctctgcc 
      acaggcgag cactggctg ggtaacagg aacttgcac 
      tacctcacc cagaaaaac tgcaccagg ttgcaaatc 
      aacatgaag gacatctac gggaagtac tggcaagcc 
      gagtacgag gagttcaac gtgggggac tacagcatg 
      gggttcaag cttgtggtg ggtggctac agtgggaat 
      gcgagtgat gcacttgat taccaaaac catatggaa 
      ttttcgacg attgataac gatcgtgat atttccaat 
      acccactgt gcgagtaat tatgagggg ggttggtgg 
      ttctcccac tgccagcat gccaattta aacgggaga 
      tacaacctg ggtctgacc tggtttgat agttcgagg 
      aacgagtgg atagcggtg tcgaagagc gagatgaag 
      gtgaagcaa agagagaac tgttga
    mRNA sequence
    • >TC003194 RA
      tgtcaggtc aggaatgcg tcagcgtat aatatgtct 
      ctattgttg cagttgaaa gccgcccaa ttggaatgg 
      aatcacaat tggcaaatt ttgaacgag aaaaaacca 
      acaatggac tttgaatct ccggaccaa acccaccca 
      tcacgccat caaagactc ctcaaaaaa cacgtcctc 
      gacctggag aagacgtca aaactcctc catcgcgag 
      aatgaacgt ctgaaaacg cgcttcttg aaagtggag 
      gaggaatta gacctattg cagaaatac gtcgagaat 
      aaggacgaa aggatcaac ggcaacgaa atccaggac 
      tacatcaac cgggagcgc gtccaggaa gtcggccgg 
      gatgagttc ggccgtcgc ttgttgaaa aacagcgaa 
      agcatcgcc aatttgact aaacaaatg tcgaatttc 
      gataaatta cacttgtcc atgttggag ttgttggaa 
      aacgtcgaa agtatcgag aataaggtc gatgtgggt 
      ctgcctgac ttccgcaaa gagatctcg aaattggaa 
      attcaaatc ggcgatgcc gtttcgcag atttctttg 
      ttaagggaa gatcaaaca aatacgaga cagtcggtt 
      aaagccatc ggggtgagt gtgtccaat ttgcaagat 
      aaagccgac acggagcga aaacgagtg cggaaagtc 
      gaggagcag cttgagagt ttgaccaaa gcggcttcc 
      atgcagacg gccaagttg cacgatcat attttgaag 
      accgagtcg tcgacactc gacaccaac cagaccaag 
      tccaccatc cacctggtg caggaactg aaatcgttc 
      gaaaacgag tacaagagc atcgtcaat aagttacca 
      cacgattgt ggcgcggtt tctggacct agtggagtc 
      tatttgatt tcaccagga gatggtgaa ccgatcctg 
      gcttattgc accaacggc tggaccacc atccagaaa 
      cgctacgac ggttcggtg gatttcaac cgcaactgg 
      aacgactac tccaacggg ttcggctct gccacaggc 
      gagcactgg ctgggtaac aggaacttg cactacctc 
      acccagaaa aactgcacc aggttgcaa atcaacatg 
      aaggacatc tacgggaag tactggcaa gccgagtac 
      gaggagttc aacgtgggg gactacagc atggggttc 
      aagcttgtg gtgggtggc tacagtggg aatgcgagt 
      gatgcactt gattaccaa aaccatatg gaattttcg 
      acgattgat aacgatcgt gatatttcc aatacccac 
      tgtgcgagt aattatgag gggggttgg tggttctcc 
      cactgccag catgccaat ttaaacggg agatacaac 
      ctgggtctg acctggttt gatagttcg aggaacgag 
      tggatagcg gtgtcgaag agcgagatg aaggtgaag 
      caaagagag aactgttga attttttcc aaatgttta 
      tcaatgtta accagtgta tgccatgct ataattgta 
      tttttagtg gattgggaa gactgaaat ttttgttcg 
      agttttttt gccagtatt taccaaaga aaagactag 
      ttcattcga attgttagg ttaagcgca cactttttc 
      agataatat gtcgtgtat atatatttt tattattta 
      ttcgtatgc ttttttact gccatatag gtttctaac 
      attgtatat actagtatt tattgtaat ttataatgt 
      ttgacaaag atagggata attttccac tttgatggt 
      ttatgtaaa tatacttag gtcgatacc tttgtacca 
      atgattagt tttttaata aaattatta aggaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY