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Gene information
General information
TC003167 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 818984..821571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003167
    iBeetle screen iB_03513
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003167 RA
      MGPPKRYSS KAEGKSKKR KKEEEEWTY EEDDVNESE 
      GAAVPGAAA RDAEKNDQS VQEDEFGAK DYRSQMILK 
      PDNTSRPLW VAPNGHIFL ESFSPVYKH AHDFLIAIS 
      EPVCRPEHI HEYKLTAYS LYAAVSVGL QTNDIIEYL 
      NRLSKTSIP DGIVEFIKL CTLSYGKVK LVLKHNKYF 
      VESPHPEIL QKLLKDPVI QECRLRRNE EGDEFITQV 
      QEKKNIPSF GAKPTPGDQ PDSSAVPDD ITNFYDKID 
      NEEDEEEAT LQTVSFEVN QEKIETIQK RCIELEYPL 
      LAEYDFRND TINPDINVD LRPAAVLRP YQEKSLRKM 
      FGNGRARSG VIVLPCGAG KSLVGVTAC CTVRKRALV 
      LCNSGVSVE QWKQQFKMW STADDSMIC RFTSEAKDK 
      PMGCSILVT TYSMITHTQ RRSWEAEQT MKWLQEQEW 
      GIMVLDEVH TIPAKMFRR VLTIVQSHC KLGLTATLL 
      REDDKIADL NFLIGPKLY EANWLELQK RGFIARVQC 
      AEVWCPMTP EFYREYLGC KTSKRLLLY VMNPNKFRA 
      TQYLIRYHE RRGDKTIVF SDNVFALKH YAIKMNKPY 
      IYGPTSQGE RIQILQNFK FNPKVNTIF VSKVADTSF 
      DLPEANVLI QISSHGGSR RQEAQRLGR ILRAKKGAI 
      AEEYNAFFY TLVSQDTME MNYSRKRQR FLVNQGYSY 
      KVITKLAGM DEEPDLMYK TREEQGQLL QQVLAASDI 
      DCEDEKVPG EGGRGAGGS GNRRTGQMG SMSGADDAV 
      YYEYKKNAA NQHKHPLFK KFRY
    CDS sequence
    • >TC003167 RA
      atgggtcca cctaaacgc tacagtagc aaagctgaa 
      ggaaaatca aaaaaacgc aaaaaggag gaagaagaa 
      tggacgtac gaagaagac gatgtcaat gaatctgaa 
      ggggctgct gttccgggt gctgcggcc cgtgacgct 
      gagaaaaac gaccaaagc gtccaagag gacgaattc 
      ggagccaaa gactaccgt tctcagatg attctaaaa 
      cctgataac acgtcacga ccgttatgg gtcgccccc 
      aatggtcat atttttctc gagtcgttt tcaccggtt 
      tataaacac gctcatgat tttctaatt gcgatttcg 
      gagcccgtt tgccgtccg gagcacatt cacgagtat 
      aaactaacg gcttattcg ttatatgcc gccgtgagt 
      gtggggctg cagactaac gacataatc gagtattta 
      aaccggttg agtaaaacg agtattccg gacggtatt 
      gtcgaattc attaaactg tgtacgcta tcgtatggg 
      aaagtcaag ctagttttg aagcacaat aaatatttc 
      gtggagagc ccccatccg gagattctc caaaaacta 
      ttgaaagac ccggtgatt caagaatgt cgtttgagg 
      cgcaacgag gaaggggac gagtttata acgcaggtg 
      caagaaaag aaaaacatt ccgagcttt ggtgccaaa 
      ccaaccccc ggtgaccaa cccgattcg agcgcagtc 
      cctgacgac attacaaat ttctacgac aaaatcgac 
      aacgaagaa gatgaagaa gaagcaact cttcaaact 
      gtctcattc gaagtcaat caggaaaaa atcgaaaca 
      atacaaaaa cgttgcatt gaactcgaa tacccttta 
      ttggctgaa tacgatttc cgaaacgat acaattaac 
      cctgacatt aacgttgat ttgcgccca gcggctgta 
      cttcgcccc tatcaggaa aaaagcctc cgaaaaatg 
      ttcggtaac ggacgggca cggtcgggt gttatcgtg 
      ttaccttgc ggtgccggt aaatccctg gtgggcgta 
      acggcatgt tgtacggta cgcaaacga gcgctggtt 
      ttatgtaat tccggtgtt tccgtcgaa caatggaaa 
      caacaattt aaaatgtgg tcaacagct gatgatagt 
      atgatttgt cggtttact tccgaagct aaggataaa 
      ccaatgggg tgttccatt ctcgtaaca acctattcc 
      atgataacg cacacgcag cgaaggtca tgggaggct 
      gaacagacc atgaaatgg ttacaggag caggaatgg 
      gggattatg gtacttgat gaagttcat accattcct 
      gccaaaatg tttcgacgg gttttaact atcgttcag 
      tcgcattgt aagctaggt ttgacggcg actttactg 
      cgagaggat gataaaatt gccgatttg aattttttg 
      attggtcca aaattgtac gaagccaac tggttggag 
      ttacaaaaa agagggttt atagcaagg gttcagtgt 
      gctgaagtt tggtgcccg atgacaccc gaattttat 
      agagagtat ctcggttgt aaaacaagc aagagactt 
      ttgttatac gtcatgaat ccgaataaa ttccgagct 
      acgcaatac ttgatacgt tatcacgaa agaagaggt 
      gacaaaact atagttttt tcagataat gttttcgct 
      ttgaaacac tacgccatc aagatgaat aaaccgtat 
      atttatggg cccacctct caaggtgaa aggattcaa 
      attttgcaa aacttcaaa tttaatccg aaagttaac 
      acgattttt gtgagtaag gtggctgat acgtccttt 
      gatttaccc gaagccaac gttttgatt caaatttcg 
      tcacatgga gggtctagg aggcaagaa gcccaacgt 
      ttagggagg attttaagg gcgaaaaaa ggtgcaatc 
      gctgaggaa tataacgcc ttcttttac actctcgta 
      tcacaagat acaatggaa atgaattac tctaggaag 
      cgacagagg tttttggtc aatcaaggt tacagttac 
      aaggttatt accaagttg gcaggcatg gatgaagag 
      cctgatttg atgtacaaa acgagggaa gagcaggga 
      cagttgttg cagcaagtt ttggctgcg agtgatatc 
      gactgtgag gatgagaaa gttccaggt gaaggtggt 
      agaggagct gggggctca gggaatcgc aggacggga 
      caaatgggg tccatgtca ggagctgat gatgccgtt 
      tattacgag tataagaag aatgcggcg aatcagcat 
      aaacatcca ttgtttaag aaattcaga tattaa
    mRNA sequence
    • >TC003167 RA
      aaacgtcac taacctatg aaagtgtaa acttgtgca 
      acactttaa atgtgaaaa acaatcgga aaaaacgca 
      aatcaaact gtgaaaatg ggtccacct aaacgctac 
      agtagcaaa gctgaagga aaatcaaaa aaacgcaaa 
      aaggaggaa gaagaatgg acgtacgaa gaagacgat 
      gtcaatgaa tctgaaggg gctgctgtt ccgggtgct 
      gcggcccgt gacgctgag aaaaacgac caaagcgtc 
      caagaggac gaattcgga gccaaagac taccgttct 
      cagatgatt ctaaaacct gataacacg tcacgaccg 
      ttatgggtc gcccccaat ggtcatatt tttctcgag 
      tcgttttca ccggtttat aaacacgct catgatttt 
      ctaattgcg atttcggag cccgtttgc cgtccggag 
      cacattcac gagtataaa ctaacggct tattcgtta 
      tatgccgcc gtgagtgtg gggctgcag actaacgac 
      ataatcgag tatttaaac cggttgagt aaaacgagt 
      attccggac ggtattgtc gaattcatt aaactgtgt 
      acgctatcg tatgggaaa gtcaagcta gttttgaag 
      cacaataaa tatttcgtg gagagcccc catccggag 
      attctccaa aaactattg aaagacccg gtgattcaa 
      gaatgtcgt ttgaggcgc aacgaggaa ggggacgag 
      tttataacg caggtgcaa gaaaagaaa aacattccg 
      agctttggt gccaaacca acccccggt gaccaaccc 
      gattcgagc gcagtccct gacgacatt acaaatttc 
      tacgacaaa atcgacaac gaagaagat gaagaagaa 
      gcaactctt caaactgtc tcattcgaa gtcaatcag 
      gaaaaaatc gaaacaata caaaaacgt tgcattgaa 
      ctcgaatac cctttattg gctgaatac gatttccga 
      aacgataca attaaccct gacattaac gttgatttg 
      cgcccagcg gctgtactt cgcccctat caggaaaaa 
      agcctccga aaaatgttc ggtaacgga cgggcacgg 
      tcgggtgtt atcgtgtta ccttgcggt gccggtaaa 
      tccctggtg ggcgtaacg gcatgttgt acggtacgc 
      aaacgagcg ctggtttta tgtaattcc ggtgtttcc 
      gtcgaacaa tggaaacaa caatttaaa atgtggtca 
      acagctgat gatagtatg atttgtcgg tttacttcc 
      gaagctaag gataaacca atggggtgt tccattctc 
      gtaacaacc tattccatg ataacgcac acgcagcga 
      aggtcatgg gaggctgaa cagaccatg aaatggtta 
      caggagcag gaatggggg attatggta cttgatgaa 
      gttcatacc attcctgcc aaaatgttt cgacgggtt 
      ttaactatc gttcagtcg cattgtaag ctaggtttg 
      acggcgact ttactgcga gaggatgat aaaattgcc 
      gatttgaat tttttgatt ggtccaaaa ttgtacgaa 
      gccaactgg ttggagtta caaaaaaga gggtttata 
      gcaagggtt cagtgtgct gaagtttgg tgcccgatg 
      acacccgaa ttttataga gagtatctc ggttgtaaa 
      acaagcaag agacttttg ttatacgtc atgaatccg 
      aataaattc cgagctacg caatacttg atacgttat 
      cacgaaaga agaggtgac aaaactata gttttttca 
      gataatgtt ttcgctttg aaacactac gccatcaag 
      atgaataaa ccgtatatt tatgggccc acctctcaa 
      ggtgaaagg attcaaatt ttgcaaaac ttcaaattt 
      aatccgaaa gttaacacg atttttgtg agtaaggtg 
      gctgatacg tcctttgat ttacccgaa gccaacgtt 
      ttgattcaa atttcgtca catggaggg tctaggagg 
      caagaagcc caacgttta gggaggatt ttaagggcg 
      aaaaaaggt gcaatcgct gaggaatat aacgccttc 
      ttttacact ctcgtatca caagataca atggaaatg 
      aattactct aggaagcga cagaggttt ttggtcaat 
      caaggttac agttacaag gttattacc aagttggca 
      ggcatggat gaagagcct gatttgatg tacaaaacg 
      agggaagag cagggacag ttgttgcag caagttttg 
      gctgcgagt gatatcgac tgtgaggat gagaaagtt 
      ccaggtgaa ggtggtaga ggagctggg ggctcaggg 
      aatcgcagg acgggacaa atggggtcc atgtcagga 
      gctgatgat gccgtttat tacgagtat aagaagaat 
      gcggcgaat cagcataaa catccattg tttaagaaa 
      ttcagatat taataaagt ttttcagtt ctggttcgt 
      tgtctcatt tctagtgct ttcttcgtt ctaatatta 
      tagcgagtt gtatactat ctt