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Gene information
General information
TC003158 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 1032257..1044788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003158
    iBeetle screen iB_03511
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    remarks: later than schedule,
    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 4

    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003158 RA
      MEKYPLKRA GTAVPQNSP SYQSVDKKY NTNRAAFAR 
      SSDNFDAPA HGDDMIDIT TSGGGGERT TRLGEDEDG 
      TSHYSVSFG GMQGETVDI PGIGQYDDF HTIDWQRDI 
      ARDRMRHRH LVKKKQDSI WNLIEGAHD AWSGWLCVL 
      LVGLCTGMV AGGIDIGAS WMTDLKFGI CPQAFWLNR 
      EQCCWSSNE TSFDSGNCS QWLTWPEVL GQSRDGAGA 
      YIISYLFYI VLALLFAAL SASLVRMFA PYACGSGIP 
      EIKTILSGF IIRGYLGKW TLVIKCVGL ILSVSAGLS 
      LGKEGPMVH IACAIGNIL SYLFPKYGR NEAKKREIL 
      SASAAAGVS VAFGAPIGG VLFSLEEVS YYFPLKTLW 
      RSFFCALIA AFILRSINP FGNEHSVLF YVEYNKPWI 
      FFELIPFIG LGIIGGIIA TIFIKANLY WCRYRKYSK 
      LGQYPVTEV LVVTVMTAI IAYPNPYTR MNTSQLIYL 
      LFSQCGISN SDNLCDYNR NFTDVNSAI EIAAAGPGV 
      YKAIWLLIL ALILKLIMT IFTFGMKVP CGLFIPSLC 
      LGAIVGRIV GIGMEQLAY NYPKNWLFS GECSTGDDC 
      ITPGLYAMV GAAAVLGGV TRMTVSLVV IMFELTGGV 
      RYIVPLMAA AMASKWVGD ALGRQGIYD AHIALNGYP 
      FLDSKDEFQ HTSLAADVM QPKRNETLS VITQDSMTV 
      DDIEALLKE TEHNGYPVV VSRESQYLV GFVLRRDLN 
      LAIANAKRM ADGICGQSI VLFTSGNPP QTLGPPPLK 
      LKKILDMAP ITITDQTPM ETVVDMFRK LGLRQTLVT 
      HNGRLLGVI TKKDVLRHI KQMDNEDPN SVLFN
    CDS sequence
    • >TC003158 RA
      atggaaaaa tatccattg aaaagggcc ggtacggca 
      gttccacag aactcacca agctatcag tcagttgat 
      aagaagtac aatacgaac agagctgct ttcgcacga 
      tcgtcggat aatttcgat gcaccagca catggtgac 
      gatatgatc gacattacg acttctgga ggcggcggc 
      gagaggacg acgaggttg ggggaggat gaggatggc 
      acatcacat tattcggtc tcgttcggt ggaatgcaa 
      ggagaaacg gtcgatatt ccgggcatt ggccaatat 
      gacgatttc cacacgatc gattggcaa agggatatc 
      gccagagat cgcatgcga catcgacat ctcgtcaag 
      aaaaaacaa gactctatt tggaaccta attgagggg 
      gcacatgac gcctggtca ggctggctg tgtgtcctt 
      ttagtaggt ctctgtact ggtatggtg gctggaggg 
      attgatatt ggagctagt tggatgaca gatcttaaa 
      tttggtatt tgtcctcaa gcgttttgg ttgaacaga 
      gagcagtgt tgttggtcg tcgaatgag acgagtttt 
      gattcgggg aattgctca cagtggctg acgtggcct 
      gaagtcctg ggccagtca agagatgga gccggtgct 
      tacatcatt tcctatctc ttttacata gttttggcg 
      ttacttttt gccgcatta tcggcctct ttagtgaga 
      atgtttgca ccatatgct tgcgggtct ggtatacca 
      gagatcaag accatactg agcggtttt ataatacgg 
      ggttatttg ggcaaatgg actttagtc atcaaatgt 
      gtgggcttg attttatcg gtttcggcc ggtttgagt 
      ctcgggaaa gaaggacca atggttcat atagcctgc 
      gccataggt aatatttta tcatatctc tttcccaag 
      tacgggagg aatgaagca aagaaaagg gagattttg 
      tcggcttcg gcagctgca ggagtttcg gtggctttc 
      ggagcgccc attggagga gtcctcttt agtcttgaa 
      gaggtcagc tattatttc ccgctgaaa acactttgg 
      agatcgttc ttctgcgcc ctaatcgcc gcctttatc 
      ttaaggtcg atcaatcct tttggaaac gaacattcg 
      gtactgttc tacgttgaa tacaacaaa ccatggata 
      tttttcgaa ttgattcct ttcatcggc ttgggaatt 
      atcggaggt atcatcgca acgatattc atcaaggcc 
      aacttgtac tggtgccgg taccggaag tactccaaa 
      ctcggtcaa tatcctgtg actgaggtg ctagtcgtg 
      accgtaatg acggccatc atcgcctac cccaacccc 
      tacactcgc atgaacacg agccaattg atttatctt 
      ctgttcagc cagtgcggc atctccaac tcggataat 
      ctctgcgat tacaatcgc aacttcacc gatgtcaac 
      tcagccatc gaaatcgcg gcagccggc cctggcgtc 
      tacaaagcc atctggctc ctaatcctc gccctaatc 
      ctcaaacta atcatgacg atcttcacc ttcggcatg 
      aaagtcccc tgcggttta ttcatcccg agtctttgt 
      ttgggagcg atcgtgggc cgaatagtg ggcattgga 
      atggaacaa ctcgcctac aactacccc aaaaattgg 
      ctattcagt ggggaatgt tcaacgggc gatgattgc 
      attacacca ggtctatat gcaatggta ggagcggct 
      gcagtgttg ggaggtgtc accagaatg accgtttcg 
      ctggtggtg atcatgttt gagctgaca ggaggtgtg 
      cgatatatt gtgccctta atggcagcc gctatggcc 
      agtaaatgg gtgggtgat gccttgggt aggcaaggt 
      atttacgat gcgcatatt gccctgaat gggtacccg 
      tttttggac tcgaaagat gagtttcaa cacacgtcg 
      ctggcggcg gatgttatg caaccgaaa cgcaacgaa 
      acgttgagc gtcatcacg caggattcg atgacagtg 
      gacgacatc gaagctctg ttgaaagag acagaacat 
      aacggatat cctgttgtg gtatctcga gaatctcaa 
      tatttagtg ggttttgtc cttcgaagg gatttgaat 
      ttggcgatt gccaatgcg aaacgaatg gctgacgga 
      atttgcggc cagtcaata gtgctcttc actagtggg 
      aatccccca cagacttta ggaccacca cctttgaag 
      cttaaaaag atcctagat atggcacca atcaccata 
      actgatcaa actccaatg gaaactgtt gtagatatg 
      tttaggaaa ttggggttg aggcaaaca ctagtcaca 
      cataacgga agattacta ggagttatc acgaagaag 
      gacgtccta aggcatata aaacagatg gataatgaa 
      gacccgaat tcggttctg tttaattaa
    mRNA sequence
    • >TC003158 RA
      aaaaaatga caggtgtga ttaatccga tgtgacgtc 
      ttgtaaaaa aaataccat aatttacag tgtgtttta 
      tctaaatta aatgaaacg attaaagta ctgataagg 
      gataaaaat ctacacaat ggtgtacaa atgaatgat 
      ggaaaaata tccattgaa aagggccgg tacggcagt 
      tccacagaa ctcaccaag ctatcagtc agttgataa 
      gaagtacaa tacgaacag agctgcttt cgcacgatc 
      gtcggataa tttcgatgc accagcaca tggtgacga 
      tatgatcga cattacgac ttctggagg cggcggcga 
      gaggacgac gaggttggg ggaggatga ggatggcac 
      atcacatta ttcggtctc gttcggtgg aatgcaagg 
      agaaacggt cgatattcc gggcattgg ccaatatga 
      cgatttcca cacgatcga ttggcaaag ggatatcgc 
      cagagatcg catgcgaca tcgacatct cgtcaagaa 
      aaaacaaga ctctatttg gaacctaat tgagggggc 
      acatgacgc ctggtcagg ctggctgtg tgtcctttt 
      agtaggtct ctgtactgg tatggtggc tggagggat 
      tgatattgg agctagttg gatgacaga tcttaaatt 
      tggtatttg tcctcaagc gttttggtt gaacagaga 
      gcagtgttg ttggtcgtc gaatgagac gagttttga 
      ttcggggaa ttgctcaca gtggctgac gtggcctga 
      agtcctggg ccagtcaag agatggagc cggtgctta 
      catcatttc ctatctctt ttacatagt tttggcgtt 
      actttttgc cgcattatc ggcctcttt agtgagaat 
      gtttgcacc atatgcttg cgggtctgg tataccaga 
      gatcaagac catactgag cggttttat aatacgggg 
      ttatttggg caaatggac tttagtcat caaatgtgt 
      gggcttgat tttatcggt ttcggccgg tttgagtct 
      cgggaaaga aggaccaat ggttcatat agcctgcgc 
      cataggtaa tattttatc atatctctt tcccaagta 
      cgggaggaa tgaagcaaa gaaaaggga gattttgtc 
      ggcttcggc agctgcagg agtttcggt ggctttcgg 
      agcgcccat tggaggagt cctctttag tcttgaaga 
      ggtcagcta ttatttccc gctgaaaac actttggag 
      atcgttctt ctgcgccct aatcgccgc ctttatctt 
      aaggtcgat caatccttt tggaaacga acattcggt 
      actgttcta cgttgaata caacaaacc atggatatt 
      tttcgaatt gattccttt catcggctt gggaattat 
      cggaggtat catcgcaac gatattcat caaggccaa 
      cttgtactg gtgccggta ccggaagta ctccaaact 
      cggtcaata tcctgtgac tgaggtgct agtcgtgac 
      cgtaatgac ggccatcat cgcctaccc caaccccta 
      cactcgcat gaacacgag ccaattgat ttatcttct 
      gttcagcca gtgcggcat ctccaactc ggataatct 
      ctgcgatta caatcgcaa cttcaccga tgtcaactc 
      agccatcga aatcgcggc agccggccc tggcgtcta 
      caaagccat ctggctcct aatcctcgc cctaatcct 
      caaactaat catgacgat cttcacctt cggcatgaa 
      agtcccctg cggtttatt catcccgag tctttgttt 
      gggagcgat cgtgggccg aatagtggg cattggaat 
      ggaacaact cgcctacaa ctaccccaa aaattggct 
      attcagtgg ggaatgttc aacgggcga tgattgcat 
      tacaccagg tctatatgc aatggtagg agcggctgc 
      agtgttggg aggtgtcac cagaatgac cgtttcgct 
      ggtggtgat catgtttga gctgacagg aggtgtgcg 
      atatattgt gcccttaat ggcagccgc tatggccag 
      taaatgggt gggtgatgc cttgggtag gcaaggtat 
      ttacgatgc gcatattgc cctgaatgg gtacccgtt 
      tttggactc gaaagatga gtttcaaca cacgtcgct 
      ggcggcgga tgttatgca accgaaacg caacgaaac 
      gttgagcgt catcacgca ggattcgat gacagtgga 
      cgacatcga agctctgtt gaaagagac agaacataa 
      cggatatcc tgttgtggt atctcgaga atctcaata 
      tttagtggg ttttgtcct tcgaaggga tttgaattt 
      ggcgattgc caatgcgaa acgaatggc tgacggaat 
      ttgcggcca gtcaatagt gctcttcac tagtgggaa 
      tcccccaca gactttagg accaccacc tttgaagct 
      taaaaagat cctagatat ggcaccaat caccataac 
      tgatcaaac tccaatgga aactgttgt agatatgtt 
      taggaaatt ggggttgag gcaaacact agtcacaca 
      taacggaag attactagg agttatcac gaagaagga 
      cgtcctaag gcatataaa acagatgga taatgaaga 
      cccgaattc ggttctgtt taattaaat aaattttat 
      tgtgtatat tatggttta ttaatattt gtgcatatt 
      agtattgta ttgttaatc ccctattat ttattacgg 
      actttttac ttctattgt gacgtgatt ttcaacgta 
      ttgtcttcc tgccgtaat tttattaac ttgcagcct 
      tgttttgcg attaaagtt atttaatca aattagttg 
      tcaataatg ctcagatta taataatat atataataa 
      aaattttct aaagaa