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Gene information
General information
TC003157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1044830..1048671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003157
    iBeetle screen iB_03510

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003157 RA
      MDTAIKEIE EKEASPSES VETEELDIE SEPFFQDIY 
      MLTEHGVPL PDIEEMRNI GINTVKGLQ MTTTDKLLA 
      LKSFNPSKV SKIQEICGN ISFSNRFMT AFEMETCNL 
      WSPKSRIFF IIAGTVNNF SVIEESLEI IWSRKILNF 
      VLVFVNTQI NVLSYNGFA QEKIVNLTN SRDFFPNKL 
      LDINGHVLK IGMFKDTPR NTKNSKGQW YGPDYELLE 
      AITFMMNAT LQIVESAQE EHFNGLYDN VLNGIVDFS 
      FIPLYKFDT FSQIDFSYP RKLENLVVL VPKAGQIPQ 
      ESYLFLIFD YKIWLSVLV FLFLISCVL KQAQFVVKK 
      RQNSFIYWL FEAWKCFIG SGGTTSFGR KETPVKFIL 
      TVWMLGSII VSACFQCSF TSSFTKPKY FDEINTLDD 
      LRQTGLRIF CADYYKNYL LKVESYGLH TQLVFVSTR 
      KMYEIRKGK DAGKVSFVE VFSRAVILA KLYGYHIVK 
      EPLATGYTV NYFQKNSPY MDEVHRCVL LDQEVGLSK 
      KADQNLIIP PDHEDAITS VNITFSHLK SLFLILLCG 
      YHVSFVVFL CEFCVKAKN KF
    CDS sequence
    • >TC003157 RA
      atggacaca gctataaaa gaaattgaa gaaaaagaa 
      gctagtcct tctgaatct gttgaaacg gaggaattg 
      gacatcgaa agcgaacct ttctttcag gatatttac 
      atgctgacc gaacacggc gtccctctt ccagatatc 
      gaagaaatg cgaaacatt ggcataaac acagtcaag 
      ggcctccaa atgaccaca accgacaaa cttttggct 
      ttgaaaagc tttaatccg agtaaagta agcaaaatt 
      caggaaatt tgcggaaat atctctttc agcaatcgc 
      ttcatgacc gcttttgaa atggagact tgcaacttg 
      tggtcacct aaatccagg atatttttc attatcgct 
      ggaacagtc aacaatttt agtgtgatt gaggaaagt 
      ttggaaata atatggtcc cgaaaaatc ctaaacttc 
      gttctggtc ttcgtcaac acccaaata aacgtcctg 
      tcctacaac ggtttcgca caggaaaaa atcgtcaac 
      ttaacaaat tcccgggat tttttcccc aacaaattg 
      cttgacatc aacggacac gtcctaaag atcggaatg 
      tttaaagac acgccaaga aacacgaaa aattccaaa 
      gggcagtgg tacggtcct gactacgag ctccttgaa 
      gccatcaca tttatgatg aatgcaact ttgcaaatt 
      gttgaatcg gcgcaagag gaacatttt aacgggctt 
      tatgacaac gttttgaac ggaattgtt gattttagt 
      ttcattccg ttgtacaaa ttcgatacg ttctcacaa 
      atcgatttt tcctatcca aggaaattg gaaaatttg 
      gtggttttg gtgcccaaa gctgggcaa ataccacaa 
      gagagttat ttatttttg atttttgac tacaaaata 
      tggttgagt gttttagtg tttcttttt ttgatcagt 
      tgtgtctta aaacaggca caatttgtg gtaaagaag 
      cgacaaaat tcgttcatt tactggctg tttgaagcc 
      tggaagtgt ttcatagga agtggagga acgacaagt 
      tttgggcga aaagaaacg ccagttaaa ttcatttta 
      acggtttgg atgttgggt tctattatt gtgagtgcg 
      tgctttcaa tgctcattt acttcatcg ttcactaaa 
      ccgaaatac ttcgatgaa attaacact ttggacgat 
      ttgagacaa acaggattg agaattttt tgtgccgat 
      tattacaaa aattatctc ctaaaagtt gaaagttac 
      ggtcttcac acacagtta gttttcgtg agtacacga 
      aaaatgtac gaaattagg aaaggtaaa gacgctggg 
      aaagtttcg tttgttgag gtttttagc cgtgctgtt 
      atcttggcg aaactttac ggttaccac attgttaaa 
      gaaccactg gcaacgggg tacacggtc aattatttc 
      cagaaaaac tcgccttac atggacgaa gtccatcgg 
      tgtgtttta ctggaccag gaagtgggt ttatcgaaa 
      aaagctgac caaaaccta attattcca cccgaccat 
      gaggacgca atcacaagt gttaatata acgttctcc 
      catttgaaa agtctattt ttgatactg ttgtgtggc 
      taccacgtc agttttgtc gttttcctt tgtgaattt 
      tgtgtaaaa gcgaaaaat aaattttaa
    mRNA sequence
    • >TC003157 RA
      atggacaca gctataaaa gaaattgaa gaaaaagaa 
      gctagtcct tctgaatct gttgaaacg gaggaattg 
      gacatcgaa agcgaacct ttctttcag gatatttac 
      atgctgacc gaacacggc gtccctctt ccagatatc 
      gaagaaatg cgaaacatt ggcataaac acagtcaag 
      ggcctccaa atgaccaca accgacaaa cttttggct 
      ttgaaaagc tttaatccg agtaaagta agcaaaatt 
      caggaaatt tgcggaaat atctctttc agcaatcgc 
      ttcatgacc gcttttgaa atggagact tgcaacttg 
      tggtcacct aaatccagg atatttttc attatcgct 
      ggaacagtc aacaatttt agtgtgatt gaggaaagt 
      ttggaaata atatggtcc cgaaaaatc ctaaacttc 
      gttctggtc ttcgtcaac acccaaata aacgtcctg 
      tcctacaac ggtttcgca caggaaaaa atcgtcaac 
      ttaacaaat tcccgggat tttttcccc aacaaattg 
      cttgacatc aacggacac gtcctaaag atcggaatg 
      tttaaagac acgccaaga aacacgaaa aattccaaa 
      gggcagtgg tacggtcct gactacgag ctccttgaa 
      gccatcaca tttatgatg aatgcaact ttgcaaatt 
      gttgaatcg gcgcaagag gaacatttt aacgggctt 
      tatgacaac gttttgaac ggaattgtt gattttagt 
      ttcattccg ttgtacaaa ttcgatacg ttctcacaa 
      atcgatttt tcctatcca aggaaattg gaaaatttg 
      gtggttttg gtgcccaaa gctgggcaa ataccacaa 
      gagagttat ttatttttg atttttgac tacaaaata 
      tggttgagt gttttagtg tttcttttt ttgatcagt 
      tgtgtctta aaacaggca caatttgtg gtaaagaag 
      cgacaaaat tcgttcatt tactggctg tttgaagcc 
      tggaagtgt ttcatagga agtggagga acgacaagt 
      tttgggcga aaagaaacg ccagttaaa ttcatttta 
      acggtttgg atgttgggt tctattatt gtgagtgcg 
      tgctttcaa tgctcattt acttcatcg ttcactaaa 
      ccgaaatac ttcgatgaa attaacact ttggacgat 
      ttgagacaa acaggattg agaattttt tgtgccgat 
      tattacaaa aattatctc ctaaaagtt gaaagttac 
      ggtcttcac acacagtta gttttcgtg agtacacga 
      aaaatgtac gaaattagg aaaggtaaa gacgctggg 
      aaagtttcg tttgttgag gtttttagc cgtgctgtt 
      atcttggcg aaactttac ggttaccac attgttaaa 
      gaaccactg gcaacgggg tacacggtc aattatttc 
      cagaaaaac tcgccttac atggacgaa gtccatcgg 
      tgtgtttta ctggaccag gaagtgggt ttatcgaaa 
      aaagctgac caaaaccta attattcca cccgaccat 
      gaggacgca atcacaagt gttaatata acgttctcc 
      catttgaaa agtctattt ttgatactg ttgtgtggc 
      taccacgtc agttttgtc gttttcctt tgtgaattt 
      tgtgtaaaa gcgaaaaat aaattttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY