Status
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Gene information
General information
TC034574 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1117762..1128255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034574
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03509

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034574 RA
      MTVLNTTVE AVRSANSAS SQCQEIGIM SHLLVQIIF 
      YILYSAIFL LGLFGNILV CYIVYSNKA MQTVTNLFI 
      TNLALSDIL CVLCVPFTP LYTFLRKWI FGSLTCHLV 
      GYAQATSVY ISTLTLTAI AIDRFFVIL HPLRQRMKL 
      HTCLLILLG IWVFSMLVT LPYGICMQV EVESEGCIV 
      CEENWPYEN FEVAFGSFT LVMQFIIPF CIIGYCYSR 
      ISGRLNERA KSKPGTKSA RREEADKEK KKRTNRMLI 
      AMVTIFGIC WFPLNLINV VHDYYVFER GSYFHLFFF 
      LAHCLAMSS TCYNPFLYA WLNENFRKE FKVILPCIL 
      RSATNRYCN GDERTLDTL LHSTVYTSP AKHLEPREP 
      IAEPIPQTQ EYNLTATNE EWNQASVNA THDIIHNPL 
      VQSIFFMVY TTIFVLGIF GNVLVCYVV FRSRAMQTV 
      TNLFITNLA LSDILLCVL AVPFTPLYT FLGEWVFGS 
      VICHLVSYA QGASVYIST LTLMSIAID RFFVIIYPF 
      HPRMKISTC IFIIVIIWV FSILVTLPY GIYMTYFQG 
      NSTDTTTKV KYYCDENWP SEKWRKLFG GFTTTMQFI 
      FPFFIIKFC YICVSIKLN DRARSKPGS KNSRKEEAD 
      RERKRRTNR MLIAMVAIF LISWLPLNL INLINDFYD 
      QIGNWEYYL LSFFLVHAL AMSSTCYNP FLYAWLNEN 
      FRKEFKQVL PCCDSGSMR VPPGRLGNW RSERTCNGN 
      NETQQESLL QSGVHRAAS IRERKSTPP PLKTDSVEV 
      ENILVPNAN SGVPGAVYD SAAETVRVI VDYSIATLD
    CDS sequence
    • >TC034574 RA
      atgacagtg ttaaacaca accgttgag gccgtaaga 
      tcagcaaat agtgcgtcg tcccaatgt caagaaatc 
      gggataatg agtcatctc ctagtccaa ataatcttc 
      tacatcctt tactccgcc atatttctt ttagggcta 
      ttcggcaac attctcgta tgttacatt gtttattca 
      aacaaagcc atgcaaaca gtgacgaat ctgtttata 
      acgaacttg gctctctcg gatatcttg tgcgtcctg 
      tgtgtaccc ttcactcct ttgtacacg ttcttaaga 
      aagtggata ttcggcagt ttgacttgc catctagtc 
      ggatacgct caagcaact agcgtttac atttcaact 
      ttgaccctc actgcgatt gctattgat cgtttcttc 
      gtcattctc catcctctg cgacaacga atgaaactc 
      catacgtgt ctcctgatc ctgctgggc atctgggtg 
      ttctccatg ctggtcacc ctgccctac ggcatttgc 
      atgcaggtg gaggtggag tccgagggc tgtatcgtg 
      tgcgaggaa aactggcct tacgaaaac ttcgaagtc 
      gctttcggt tctttcacc ctcgtcatg caatttatc 
      atcccattt tgcatcatc gggtactgc tattcccga 
      atttcgggc cgtctcaac gagagggca aagtccaag 
      ccgggcact aaaagcgcg agacgcgag gaggccgac 
      aaggagaag aagaagagg accaacagg atgctcata 
      gccatggtc acaattttc ggcatctgt tggtttcct 
      ctcaatttg atcaatgtg gtgcatgat tattatgtg 
      tttgaacgg gggtcctac ttccacttg ttcttcttc 
      ctggctcat tgcctggcc atgtcttct acttgctat 
      aaccccttt ttgtacgcg tggctcaac gaaaatttc 
      cgaaaggag ttcaaagtg atcctgccg tgcattttg 
      cgaagcgca acgaatcgg tactgtaat ggtgatgag 
      cgcacttta gacactctt ttacactcc accgtgtac 
      acgtcaccg gctaaacac ttagaacca cgtgaaccg 
      atagctgaa cccattcct caaactcaa gaatataac 
      ctcaccgca accaacgaa gaatggaac caagcatct 
      gtgaacgcg acccacgac atcatccac aacccccta 
      gtgcaatcc atcttcttc atggtgtac accacgatc 
      ttcgtgtta ggcatattc ggcaacgtt ttagtttgt 
      tacgtggtg tttagaagt cgcgccatg cagacagta 
      accaacctc ttcataacc aacttggct ctatccgac 
      attttgctg tgtgtcctc gctgtgccc ttcactcct 
      ctgtacacg ttcctgggc gaatgggtg ttcgggagc 
      gtcatctgc cacctggtg tcgtacgcc cagggcgcc 
      agtgtgtac atttcaacc cttactttg atgtcaata 
      gccatcgat cgcttcttc gtcatcatc tatcctttc 
      cacccccgc atgaagatc tcaacttgc atcttcatc 
      atcgtcatc atctgggtg ttctcgatc ctggtcacc 
      ctcccatat ggaatctac atgacgtac ttccaaggc 
      aacagcact gacaccacc accaaagtc aagtactac 
      tgtgacgaa aactggcct agtgaaaag tggcgtaag 
      ctcttcggt ggtttcacc accaccatg cagttcatc 
      ttccccttc ttcatcatc aagttctgc tacatttgc 
      gtctcaatc aaactcaac gaccgtgca aggagcaaa 
      ccaggctcg aaaaactct cgcaaggag gaagccgac 
      cgcgaacgc aaacgacga actaaccgg atgctgatc 
      gccatggtg gcaattttc ctcatctcc tggctcccc 
      ctcaacttg atcaacctc atcaacgac ttctacgac 
      caaatcggc aactgggag tactaccta cttagcttt 
      ttcctagtg cacgctttg gcgatgtcc tccacgtgc 
      tacaacccg ttcttgtac gcctggctg aacgagaat 
      ttccgcaag gagtttaag caagtgttg ccgtgttgc 
      gactcgggg tcgatgcgg gtgccgcct ggacggctg 
      gggaactgg aggtcggag aggacgtgc aatgggaat 
      aacgagacg cagcaagag tcgctcctg cagagtgga 
      gtgcacaga gctgcgtct atcagggag aggaaatcg 
      acgccgcca ccgctgaaa accgacagt gtggaggtg 
      gagaatatt ctggtgcca aatgctaac agtggggtt 
      ccgggggcg gtttacgac tcggcggcc gagaccgtc 
      agagtaata gtcgactac agtattgca actctggac 
      tga
    mRNA sequence
    • >TC034574 RA
      taaaactca tccacattg aataatttc ccatttgaa 
      gaaaagaaa agtagtgct ttagacata aacagtaaa 
      aaatgacag tgttaaaca caaccgttg aggccgtaa 
      gatcagcaa atagtgcgt cgtcccaat gtcaagaaa 
      tcgggataa tgagtcatc tcctagtcc aaataatct 
      tctacatcc tttactccg ccatatttc ttttagggc 
      tattcggca acattctcg tatgttaca ttgtttatt 
      caaacaaag ccatgcaaa cagtgacga atctgttta 
      taacgaact tggctctct cggatatct tgtgcgtcc 
      tgtgtgtac ccttcactc ctttgtaca cgttcttaa 
      gaaagtgga tattcggca gtttgactt gccatctag 
      tcggatacg ctcaagcaa ctagcgttt acatttcaa 
      ctttgaccc tcactgcga ttgctattg atcgtttct 
      tcgtcattc tccatcctc tgcgacaac gaatgaaac 
      tccatacgt gtctcctga tcctgctgg gcatctggg 
      tgttctcca tgctggtca ccctgccct acggcattt 
      gcatgcagg tggaggtgg agtccgagg gctgtatcg 
      tgtgcgagg aaaactggc cttacgaaa acttcgaag 
      tcgctttcg gttctttca ccctcgtca tgcaattta 
      tcatcccat tttgcatca tcgggtact gctattccc 
      gaatttcgg gccgtctca acgagaggg caaagtcca 
      agccgggca ctaaaagcg cgagacgcg aggaggccg 
      acaaggaga agaagaaga ggaccaaca ggatgctca 
      tagccatgg tcacaattt tcggcatct gttggtttc 
      ctctcaatt tgatcaatg tggtgcatg attattatg 
      tgtttgaac gggggtcct acttccact tgttcttct 
      tcctggctc attgcctgg ccatgtctt ctacttgct 
      ataacccct ttttgtacg cgtggctca acgaaaatt 
      tccgaaagg agttcaaag tgatcctgc cgtgcattt 
      tgcgaagcg caacgaatc ggtactgta atggtgatg 
      agcgcactt tagacactc ttttacact ccaccgtgt 
      acacgtcac cggctaaac acttagaac cacgtgaac 
      cgatagctg aacccattc ctcaaactc aagaatata 
      acctcaccg caaccaacg aagaatgga accaagcat 
      ctgtgaacg cgacccacg acatcatcc acaaccccc 
      tagtgcaat ccatcttct tcatggtgt acaccacga 
      tcttcgtgt taggcatat tcggcaacg ttttagttt 
      gttacgtgg tgtttagaa gtcgcgcca tgcagacag 
      taaccaacc tcttcataa ccaacttgg ctctatccg 
      acattttgc tgtgtgtcc tcgctgtgc ccttcactc 
      ctctgtaca cgttcctgg gcgaatggg tgttcggga 
      gcgtcatct gccacctgg tgtcgtacg cccagggcg 
      ccagtgtgt acatttcaa cccttactt tgatgtcaa 
      tagccatcg atcgcttct tcgtcatca tctatcctt 
      tccaccccc gcatgaaga tctcaactt gcatcttca 
      tcatcgtca tcatctggg tgttctcga tcctggtca 
      ccctcccat atggaatct acatgacgt acttccaag 
      gcaacagca ctgacacca ccaccaaag tcaagtact 
      actgtgacg aaaactggc ctagtgaaa agtggcgta 
      agctcttcg gtggtttca ccaccacca tgcagttca 
      tcttcccct tcttcatca tcaagttct gctacattt 
      gcgtctcaa tcaaactca acgaccgtg caaggagca 
      aaccaggct cgaaaaact ctcgcaagg aggaagccg 
      accgcgaac gcaaacgac gaactaacc ggatgctga 
      tcgccatgg tggcaattt tcctcatct cctggctcc 
      ccctcaact tgatcaacc tcatcaacg acttctacg 
      accaaatcg gcaactggg agtactacc tacttagct 
      ttttcctag tgcacgctt tggcgatgt cctccacgt 
      gctacaacc cgttcttgt acgcctggc tgaacgaga 
      atttccgca aggagttta agcaagtgt tgccgtgtt 
      gcgactcgg ggtcgatgc gggtgccgc ctggacggc 
      tggggaact ggaggtcgg agaggacgt gcaatggga 
      ataacgaga cgcagcaag agtcgctcc tgcagagtg 
      gagtgcaca gagctgcgt ctatcaggg agaggaaat 
      cgacgccgc caccgctga aaaccgaca gtgtggagg 
      tggagaata ttctggtgc caaatgcta acagtgggg 
      ttccggggg cggtttacg actcggcgg ccgagaccg 
      tcagagtaa tagtcgact acagtattg caactctgg 
      actgatggc gatcttcaa agttcccaa acatttctc 
      gaagtattt acattggat ttaactctg tgtcaaggt 
      gttattcgg cgttctttg tgtacataa tattcacct 
      ctagtgata aagcttagc ctactgtgt attataatt 
      atattaatt ttgtatata ctatatatt tctaaaata 
      aatatcgtt atgtgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY