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Gene information
General information
TC003149 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1142481..1144965
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003149
    iBeetle screen iB_03508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003149 RA
      MSINLDKYR DELVSAWKD VLDAKTPTD WALFAYEGQ 
      TNVLKFVSK GSNGIEELT EDLNSGKIM YAFVKVNDP 
      KTSLIKFVL INWQGEGAN TVRKGISAN HLRDIEKFF 
      TGAHLTINA RNEEEVEPQ LIIDKVAKS GSAYSFKAP 
      RAEIIEPTG PVGTTYQRV NPIKEINAR ERDQFWRKE 
      EEEEKKRVE EERKRREME RMKNEEELR RRELEEAAK 
      RDAEMSLRN NNIDQIKQA EKEAAVESV SGSNYDDDF 
      PTRVNQSDV LRKQRNQEA HDLIAQRTI DARSVFEQH 
      TVAGQKKVP EKPVRNSIL KAQNQLSVE NKLEESDDE 
      SDQFSTIKR SPKDVEKKS VTSPTTPQE VFEVRKEEP 
      QEERVEQIT DQQFVDEYL YEFSTPGLQ ARALYDYQA 
      VDDTEISFD PGDIITNID QVDKGWWQG LAPNGTYGL 
      FPANYVELI Q
    CDS sequence
    • >TC003149 RA
      atgtcaata aatttagac aaataccgg gatgagctt 
      gtatcagct tggaaagat gtgcttgat gcgaaaaca 
      ccaacagat tgggcccta ttcgcctac gagggtcaa 
      accaacgtt ttgaaattt gtgagtaag ggttccaac 
      ggaatcgag gaactaacg gaagactta aacagcgga 
      aaaataatg tacgccttt gtgaaagtg aacgacccg 
      aaaacctct ttaattaag ttcgttcta atcaattgg 
      caaggagaa ggcgccaat acggtgcgt aaaggcata 
      tccgccaac cacttacgc gacattgag aaatttttc 
      accggcgct cacctaaca atcaacgct cgtaacgag 
      gaggaagtc gagcctcag ctcatcata gataaggtc 
      gcaaaatcg ggctcggca tacagtttc aaagccccc 
      agagccgag attatcgag cccacaggt cccgttggg 
      accacttat caacgcgtc aatcccata aaagaaatc 
      aatgctcgt gaacgggac cagttttgg cgcaaagag 
      gaggaagaa gagaagaaa cgggtcgaa gaggagcgg 
      aaacgcaga gaaatggag cgaatgaag aacgaggaa 
      gagctaagg cggcgggaa ttggaagag gcggccaaa 
      cgggatgcg gaaatgtcc ctcaggaat aacaacatt 
      gatcaaatc aaacaagcg gaaaaggag gccgccgtg 
      gagtcggtt tccggttcg aactacgac gatgatttt 
      ccaactaga gttaatcaa agcgatgtt ttgagaaaa 
      cagcgaaat caagaggcg cacgattta atagcacaa 
      agaacgatt gacgcccgg tcggtgttt gaacagcac 
      acggtggcc gggcagaaa aaagtgccc gaaaaaccg 
      gtcagaaat tccattttg aaagcacaa aatcaattg 
      agtgtggaa aataagttg gaggagagt gacgacgaa 
      agtgatcag ttttccaca attaaaagg tcaccaaaa 
      gatgtggaa aagaagagt gtgactagt cccacaacg 
      ccacaggaa gtctttgaa gtgagaaag gaagagccc 
      caagaggag cgggtggag caaattaca gatcagcaa 
      tttgttgat gagtatcta tatgagttt agcacacct 
      ggactgcag gctagagcg ttgtacgat tatcaggct 
      gtggacgat accgagatc agttttgat ccaggcgac 
      ataataacg aatattgat caagttgac aagggatgg 
      tggcaagga ctggctccc aatggtact tatggcctt 
      ttccccgct aattatgtt gagttgatc caataa
    mRNA sequence
    • >TC003149 RA
      aaagaacat ttcaaacaa tcggaaaat gctgatgga 
      tgttatgga attgcgcgt tttttcaaa tgtagggat 
      ttccgaccg tgccgttgg tagtggttt ctctcacat 
      gtcaaacgt caagtggtt gtgtgattt gttatcttt 
      gttgaattg tcttaattt tccggcttt aatgaatta 
      aaatcatgt caataaatt tagacaaat accgggatg 
      agcttgtat cagcttgga aagatgtgc ttgatgcga 
      aaacaccaa cagattggg ccctattcg cctacgagg 
      gtcaaacca acgttttga aatttgtga gtaagggtt 
      ccaacggaa tcgaggaac taacggaag acttaaaca 
      gcggaaaaa taatgtacg cctttgtga aagtgaacg 
      acccgaaaa cctctttaa ttaagttcg ttctaatca 
      attggcaag gagaaggcg ccaatacgg tgcgtaaag 
      gcatatccg ccaaccact tacgcgaca ttgagaaat 
      ttttcaccg gcgctcacc taacaatca acgctcgta 
      acgaggagg aagtcgagc ctcagctca tcatagata 
      aggtcgcaa aatcgggct cggcataca gtttcaaag 
      cccccagag ccgagatta tcgagccca caggtcccg 
      ttgggacca cttatcaac gcgtcaatc ccataaaag 
      aaatcaatg ctcgtgaac gggaccagt tttggcgca 
      aagaggagg aagaagaga agaaacggg tcgaagagg 
      agcggaaac gcagagaaa tggagcgaa tgaagaacg 
      aggaagagc taaggcggc gggaattgg aagaggcgg 
      ccaaacggg atgcggaaa tgtccctca ggaataaca 
      acattgatc aaatcaaac aagcggaaa aggaggccg 
      ccgtggagt cggtttccg gttcgaact acgacgatg 
      attttccaa ctagagtta atcaaagcg atgttttga 
      gaaaacagc gaaatcaag aggcgcacg atttaatag 
      cacaaagaa cgattgacg cccggtcgg tgtttgaac 
      agcacacgg tggccgggc agaaaaaag tgcccgaaa 
      aaccggtca gaaattcca ttttgaaag cacaaaatc 
      aattgagtg tggaaaata agttggagg agagtgacg 
      acgaaagtg atcagtttt ccacaatta aaaggtcac 
      caaaagatg tggaaaaga agagtgtga ctagtccca 
      caacgccac aggaagtct ttgaagtga gaaaggaag 
      agccccaag aggagcggg tggagcaaa ttacagatc 
      agcaatttg ttgatgagt atctatatg agtttagca 
      cacctggac tgcaggcta gagcgttgt acgattatc 
      aggctgtgg acgataccg agatcagtt ttgatccag 
      gcgacataa taacgaata ttgatcaag ttgacaagg 
      gatggtggc aaggactgg ctcccaatg gtacttatg 
      gccttttcc ccgctaatt atgttgagt tgatccaat 
      aattttcat ttgtatttc taccgtcgc ttcgcttat 
      tccattgct cataacgca tttatttag tatttttca 
      agcgcaata ttttgtcga taataggtt aagtttact 
      aaaaaatac caaaatttt atttttagg aacgtatcg 
      acacaccgt ttgtaatta ttcttgtgc aataaaacg 
      aattgtgaa gtattaatg ttttaataa attacgaac 
      ggtgtgttt tttattttt aaagcttgt cgccttttt 
      attttgatt tgccacact gccagctag ggtatgctc 
      ttctgtgcc aacttcacc aaattaaaa ccccaattt 
      gtttgttaa aattgcgtt tcaatgatt gttattata 
      aattttaat taaaaactc tatggaaca gttaactgt 
      tgataaata attttataa tattgtacg acttacttg 
      catcatagc acaaataaa catttacca agact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY