Status
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Gene information
General information
TC003147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1155658..1159565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003147
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03507

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003147 RA
        MMSPSNAQE DAKLDAMQR SWQSKRSRS LSLFEDLSS 
        DSQLLAMPQ ASCSPSTKS LFSALQPKG KEEEGEVYS 
        LASLQEQVV PEILEQVEQ QPTYTVLDA STVGDFPAV 
        YTVCSPERH QMPAVGVFV DKRIVPGFK YRVRPLPQI 
        GQDPTMHKC LFNDSALTL KSIGRGYAR RFTFEATDS 
        LNNNENYFW SDNRPEGYA FELEVISEG DKFTIFDIN 
        KEAQGTVEV LQLEGPQFE ISNVYSRQN IEKRANVRF 
        TGKVEFYET GVAKPMPLS GLALAVKYK HKGAAEIVK 
        VQNVVINKQ RYTLLPGIQ KIHRRVTVK GRDINDVPT 
        QYSMHGLEP YELPVVGTY VDPRVIPGF HYKVRPSDR 
        KEHLFGGRA LKLLSIGMG YAKRLTFEP DSLLNPDNY 
        LWSDNHPDG LGLEIRAVH KDMKFVIKA GDQILGEAT 
        VWRADKPQI EEKMEKVKT QSGKHAIEK YVHIDVMCH 
        IAIAIPGGG CDESSERLM RVYGLAVVR KEPNRNEAH 
        VIRVENVGL DSQLNVLFA QTHTELTFF PKK
      CDS sequence
      • >TC003147 RA
        atgatgtcg ccatcgaac gcccaagaa gacgccaaa 
        ttagacgca atgcaacgt tcatggcaa tcgaaaaga 
        agccgatct ttgagcctt ttcgaggac ttgtcttcc 
        gattcgcaa ctattagcc atgccccag gcctcgtgc 
        agccccagc accaagtcg cttttctcg gccctccag 
        cccaaaggc aaggaggag gagggagag gtttactcc 
        ctggcgagc ctacaggag caagttgtt cccgaaata 
        ttggaacaa gtcgaacaa caaccgaca tataccgtt 
        ttagacgcg tccacagtt ggggacttc cccgctgtt 
        tataccgtg tgttccccc gagcggcac caaatgccc 
        gcagttggg gttttcgtc gacaagcgc atagtccct 
        ggttttaaa taccgcgtc cggcctttg ccccaaatc 
        ggccaagac cccacaatg cacaagtgc ctcttcaat 
        gactctgcc ctgactttg aaaagcatc ggtcggggc 
        tacgcccgc agattcact ttcgaggcc actgatagt 
        ctcaacaac aacgaaaac tacttctgg agcgacaac 
        cggcccgaa ggctacgcg ttcgagctg gaggtcatc 
        tccgaaggg gacaagttc accatcttc gacatcaac 
        aaagaggcg caaggcacc gtggaggtg ctacagctg 
        gaaggacct caatttgaa attagtaat gtttacagc 
        aggcaaaat atcgagaaa cgggccaat gttaggttt 
        acgggtaag gttgagttt tacgaaacg ggagtggcg 
        aagccgatg cctctgagc ggactggcg ctggctgtg 
        aagtacaag cacaagggg gctgccgag atcgttaaa 
        gtccaaaat gtcgtgata aacaaacag aggtacacc 
        ctactccca ggcatccag aaaatacac aggagagtc 
        accgttaaa gggcgggac attaacgac gtccccact 
        caatattcc atgcacgga ctggagccc tacgaactc 
        cccgttgtt ggtacttat gtggatccc cgagtcatc 
        ccaggattc cactacaag gtcaggcca agtgacagg 
        aaagaacac ttgtttggt ggacgcgct ttgaagctt 
        ttaagtatc ggaatgggt tatgccaaa cgattgacc 
        ttcgaacct gactctctg ctcaaccct gacaattat 
        ctatggtct gataaccat cccgatggt ctcggtttg 
        gaaatcaga gccgttcat aaagacatg aagtttgta 
        atcaaagct ggggaccag attttgggt gaagctaca 
        gtttggcga gctgacaag cctcaaatt gaggaaaaa 
        atggagaag gttaagacg cagtctggc aagcacgcg 
        attgagaaa tacgtccac attgacgtc atgtgccac 
        attgccatt gctattccc ggtggagga tgtgacgaa 
        agcagcgag agattgatg cgcgtctat ggcttggct 
        gtggtgcga aaagagccc aacagaaat gaagctcac 
        gttattagg gtagagaat gtcggtttg gactcgcaa 
        ctgaacgtt ttgttcgca caaactcac actgaactg 
        acattcttc cccaaaaaa taa
      mRNA sequence
      • >TC003147 RA
        aatgcattt gcttcagag acgccgaag acgacgcta 
        ctgacgcta gacgcccaa agtgttcaa agtttttca 
        catacttac atttacaaa gtttttcat aaagttgtg 
        tttattttt aattaaaac agcaagatg atgtcgcca 
        tcgaacgcc caagaagac gccaaatta gacgcaatg 
        caacgttca tggcaatcg aaaagaagc cgatctttg 
        agccttttc gaggacttg tcttccgat tcgcaacta 
        ttagccatg ccccaggcc tcgtgcagc cccagcacc 
        aagtcgctt ttctcggcc ctccagccc aaaggcaag 
        gaggaggag ggagaggtt tactccctg gcgagccta 
        caggagcaa gttgttccc gaaatattg gaacaagtc 
        gaacaacaa ccgacatat accgtttta gacgcgtcc 
        acagttggg gacttcccc gctgtttat accgtgtgt 
        tcccccgag cggcaccaa atgcccgca gttggggtt 
        ttcgtcgac aagcgcata gtccctggt tttaaatac 
        cgcgtccgg cctttgccc caaatcggc caagacccc 
        acaatgcac aagtgcctc ttcaatgac tctgccctg 
        actttgaaa agcatcggt cggggctac gcccgcaga 
        ttcactttc gaggccact gatagtctc aacaacaac 
        gaaaactac ttctggagc gacaaccgg cccgaaggc 
        tacgcgttc gagctggag gtcatctcc gaaggggac 
        aagttcacc atcttcgac atcaacaaa gaggcgcaa 
        ggcaccgtg gaggtgcta cagctggaa ggacctcaa 
        tttgaaatt agtaatgtt tacagcagg caaaatatc 
        gagaaacgg gccaatgtt aggtttacg ggtaaggtt 
        gagttttac gaaacggga gtggcgaag ccgatgcct 
        ctgagcgga ctggcgctg gctgtgaag tacaagcac 
        aagggggct gccgagatc gttaaagtc caaaatgtc 
        gtgataaac aaacagagg tacacccta ctcccaggc 
        atccagaaa atacacagg agagtcacc gttaaaggg 
        cgggacatt aacgacgtc cccactcaa tattccatg 
        cacggactg gagccctac gaactcccc gttgttggt 
        acttatgtg gatccccga gtcatccca ggattccac 
        tacaaggtc aggccaagt gacaggaaa gaacacttg 
        tttggtgga cgcgctttg aagctttta agtatcgga 
        atgggttat gccaaacga ttgaccttc gaacctgac 
        tctctgctc aaccctgac aattatcta tggtctgat 
        aaccatccc gatggtctc ggtttggaa atcagagcc 
        gttcataaa gacatgaag tttgtaatc aaagctggg 
        gaccagatt ttgggtgaa gctacagtt tggcgagct 
        gacaagcct caaattgag gaaaaaatg gagaaggtt 
        aagacgcag tctggcaag cacgcgatt gagaaatac 
        gtccacatt gacgtcatg tgccacatt gccattgct 
        attcccggt ggaggatgt gacgaaagc agcgagaga 
        ttgatgcgc gtctatggc ttggctgtg gtgcgaaaa 
        gagcccaac agaaatgaa gctcacgtt attagggta 
        gagaatgtc ggtttggac tcgcaactg aacgttttg 
        ttcgcacaa actcacact gaactgaca ttcttcccc 
        aaaaaataa tctcacctc atttatact acttaac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY