Status
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Gene information
General information
TC032307
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003145,TC003146
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1161226..1165725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032307
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03506

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber broader - Number of animals showing the phenotype: 4
    Comment: distance between egg chambers seems to be broader
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032307 RA
      MDPQQPNSD DEDFLNVSA GSNPPSGKP DKMLGTSDF 
      ISLLGTRDE SYYIDKTTL LEVFLKCGR SRVLLTAPR 
      RFGKTINLL MLKTCFELQ VPPEEYKML SEDEFQIKV 
      KETANYKLF EELQLEITK NPDLMNKHF GKYPTLHIS 
      FKCDTPIAS FSDGVAFCC RILSKAFRR HNYLISEKA 
      LSQWDKNIY AKWLNPADW DERKAMDIN LISDGLRTL 
      VRYVFESFN KKVVLLIDE YDSVIMQAI LRIKAKDEL 
      TKVIQFCLG LIQAAVKND IEQHVEYAF ITGISHISG 
      MGLSGSNIK PFRFLENHS FVPYYGLTV MEIRNLLRK 
      TYQFDDQRI AETEKKFNG YSTVRGVKI YNIWSVLEY 
      ASRTQEDDF IYEWQDTRY IVGLEKAFR FPTIMKIIQ 
      TELLLYKPI EIKKYETLV VEDILQLVE IIGNVDPQN 
      VEFPRCDFF FSFLVEQGY LSFVANKPG LLVIPNEQI 
      KEKIEGKIT DSKIKVFDR NSIPTEDCR EFFLKISER 
      DCDYDDFKK FHTSLEDAL QNYAAKWIK KNEENIRRT 
      IKILIKHSL DALFVFDCK ILIYLSFIS INMDTAIKE 
      IEEKEASPS ESVETEELD IESEPFFQD IYMLTEHGV 
      PLPDIEEMR KIGINTVKG LQMTTTDKL LALKSFNPS 
      KVSKIQEIC GNISFSNRF MTAFEVSEA CKQVFKIST 
      GSANLDKLL GGGVESMSI TQVFGEAGS GKTQIAHTL 
      CVTTQIPTE DYSGGKVMF IDTERSFRP NRIRQIARR 
      FHLSEDSVL QNILYIRAY NSEHQYQIL KNVAVKFHE 
      DTGVFKLLI VDSIIALFR NDFMGRGVL LNRQQKLAE 
      TMSLLKKIS EEYNVAVFI TNQVTTSMN NKFPLLTIG 
      DDVKPVGGN ILAHSSTTR VALRKLTGN VRIAKIHDS 
      PELAEQEEA FTITNGGVQ DPILDAE
    CDS sequence
    • >TC032307 RA
      atggatcca caacaacca aattctgat gatgaagat 
      tttctcaat gtatctgcc ggtagcaac ccaccgtct 
      ggaaaacca gataagatg ctcggcaca tcggacttt 
      attagtctt ctggggacg agggatgag tcgtattac 
      atcgataaa accactctg ttagaagtt ttccttaaa 
      tgcggtcgt agccgagtg ctgctgaca gctccacgc 
      cgtttcgga aagactata aatctcttg atgcttaag 
      acttgtttt gagttgcaa gtaccacca gaagaatat 
      aagatgcta tcagaagat gaattccaa ataaaggta 
      aaagagaca gctaattac aagctgttt gaagagctt 
      cagttggaa ataactaag aatccagat ttgatgaac 
      aaacatttt ggaaaatat cctacatta cacatttca 
      ttcaaatgt gacacgcca atcgcaagt ttttctgat 
      ggagttgca ttctgttgt cggatttta agtaaagcc 
      ttcaggaga cataattat ttaatatcg gaaaaagca 
      ttaagtcaa tgggacaaa aatatatat gccaaatgg 
      ttgaacccc gccgactgg gatgaaagg aaagcaatg 
      gatataaat ttgatatct gacggtttg cgaacttta 
      gtccgttat gtctttgag agctttaac aaaaaagta 
      gtactactt atcgacgag tacgattcg gttatcatg 
      caagcaata ttgcgtata aaggcaaaa gatgaatta 
      acgaaggtc atacagttt tgtttaggt ttaatccaa 
      gctgctgtg aaaaatgac attgagcaa catgtagaa 
      tatgcattt atcaccgga atttcccat atttccgga 
      atgggtctt tctggttca aacataaag ccattcaga 
      tttctggaa aatcactct tttgttcct tactatggc 
      ttgacagtt atggaaatt cggaatttg ttaaggaag 
      acatatcaa tttgatgat cagcgaatt gctgaaaca 
      gaaaaaaaa ttcaacggt tattctaca gttcgtgga 
      gtaaagata tacaacatc tggtctgtg ctagaatat 
      gctagcaga actcaagag gatgatttt atatatgaa 
      tggcaagac accaggtat attgtaggt ctcgaaaaa 
      gcttttaga ttccctaca attatgaaa ataattcaa 
      acagaacta ctgttgtat aaacctata gagattaaa 
      aaatatgaa accttggtt gtagaggat atcctgcag 
      ttggtagag ataattgga aatgttgac cctcagaac 
      gtcgaattt ccacgttgt gactttttt ttctcattt 
      cttgttgag caaggctat ttgtcattt gtagctaac 
      aagcctggg ctgctcgta atacccaac gaacagatt 
      aaagagaaa atcgaggga aagataaca gactctaaa 
      attaaagta tttgatagg aatagtatt cctactgag 
      gactgcaga gaattcttt ctgaaaata tctgagagg 
      gattgtgat tatgatgac ttcaaaaaa tttcatacg 
      agtcttgaa gatgcttta caaaactat gcagcaaaa 
      tggattaaa aagaacgaa gagaacatc cgtcgtaca 
      ataaaaatt ttgatcaag cacagtttg gacgcgttg 
      tttgtattc gattgtaaa attctaatt tatttatcc 
      tttatttcg atcaacatg gacacagct ataaaagaa 
      attgaagaa aaagaagct agtccttct gaatctgtt 
      gaaacggag gaattggac atcgagagc gaacctttc 
      tttcaggat atttacatg ctgaccgaa cacggcgtc 
      cctcttcca gatatcgaa gaaatgcga aaaattggc 
      ataaacaca gtcaagggc ctccaaatg accacaacc 
      gacaaactt ttggctttg aagagcttt aatccgagt 
      aaagtaagc aaaattcag gaaatttgc ggaaatatc 
      tctttcagc aatcgcttc atgaccgcc tttgaagta 
      agtgaggca tgcaagcaa gtttttaaa ataagtacg 
      ggaagcgca aatttggat aaattgctg ggcgggggt 
      gtagagtcg atgtctata actcaagtt tttggagaa 
      gctggaagc ggaaaaacc caaattgcc cacactcta 
      tgtgtcacc acgcaaata ccgactgaa gactattcc 
      gggggcaaa gttatgttc atagacacg gagcgtagt 
      ttccgccca aatcgcatt cgccaaatt gcacgtcgt 
      tttcatttg agtgaagac agtgtcttg cagaatatt 
      ctctacatt agagcttat aactctgaa catcaatat 
      cagatcctc aaaaatgtt gctgtaaag tttcatgaa 
      gatacgggc gtgtttaag cttcttatt gtcgactct 
      attatagca ttattccgg aatgatttt atgggcaga 
      ggtgtgctt ctgaatagg cagcaaaaa ttggcggaa 
      acaatgtcg cttttgaaa aaaatcagt gaggaatat 
      aatgttgct gttttcata acaaatcaa gttacgaca 
      agtatgaat aataaattc ccgttgttg actatcggt 
      gatgatgtt aagcctgtc ggagggaat attttggcc 
      cattcgtct actactaga gtggctttg agaaagctt 
      acaggaaat gtgaggatt gccaagatc cacgatagt 
      cctgagctt gctgaacaa gaagaagct tttactata 
      actaatgga ggagtacaa gaccctata ttagatgcg 
      gaataa
    mRNA sequence
    • >TC032307 RA
      aaacaattt caaaagctt tttatcgtt tacaacaat 
      aatcataaa cagtacaga aaatcatca ttttacttt 
      attattatt taaattatt ttattaatt tattttact 
      tgcgcagag tctatggat ccacaacaa ccaaattct 
      gatgatgaa gattttctc aatgtatct gccggtagc 
      aacccaccg tctggaaaa ccagataag atgctcggc 
      acatcggac tttattagt cttctgggg acgagggat 
      gagtcgtat tacatcgat aaaaccact ctgttagaa 
      gttttcctt aaatgcggt cgtagccga gtgctgctg 
      acagctcca cgccgtttc ggaaagact ataaatctc 
      ttgatgctt aagacttgt tttgagttg caagtacca 
      ccagaagaa tataagatg ctatcagaa gatgaattc 
      caaataaag gtaaaagag acagctaat tacaagctg 
      tttgaagag cttcagttg gaaataact aagaatcca 
      gatttgatg aacaaacat tttggaaaa tatcctaca 
      ttacacatt tcattcaaa tgtgacacg ccaatcgca 
      agtttttct gatggagtt gcattctgt tgtcggatt 
      ttaagtaaa gccttcagg agacataat tatttaata 
      tcggaaaaa gcattaagt caatgggac aaaaatata 
      tatgccaaa tggttgaac cccgccgac tgggatgaa 
      aggaaagca atggatata aatttgata tctgacggt 
      ttgcgaact ttagtccgt tatgtcttt gagagcttt 
      aacaaaaaa gtagtacta cttatcgac gagtacgat 
      tcggttatc atgcaagca atattgcgt ataaaggca 
      aaagatgaa ttaacgaag gtcatacag ttttgttta 
      ggtttaatc caagctgct gtgaaaaat gacattgag 
      caacatgta gaatatgca tttatcacc ggaatttcc 
      catatttcc ggaatgggt ctttctggt tcaaacata 
      aagccattc agatttctg gaaaatcac tcttttgtt 
      ccttactat ggcttgaca gttatggaa attcggaat 
      ttgttaagg aagacatat caatttgat gatcagcga 
      attgctgaa acagaaaaa aaattcaac ggttattct 
      acagttcgt ggagtaaag atatacaac atctggtct 
      gtgctagaa tatgctagc agaactcaa gaggatgat 
      tttatatat gaatggcaa gacaccagg tatattgta 
      ggtctcgaa aaagctttt agattccct acaattatg 
      aaaataatt caaacagaa ctactgttg tataaacct 
      atagagatt aaaaaatat gaaaccttg gttgtagag 
      gatatcctg cagttggta gagataatt ggaaatgtt 
      gaccctcag aacgtcgaa tttccacgt tgtgacttt 
      tttttctca tttcttgtt gagcaaggc tatttgtca 
      tttgtagct aacaagcct gggctgctc gtaataccc 
      aacgaacag attaaagag aaaatcgag ggaaagata 
      acagactct aaaattaaa gtatttgat aggaatagt 
      attcctact gaggactgc agagaattc tttctgaaa 
      atatctgag agggattgt gattatgat gacttcaaa 
      aaatttcat acgagtctt gaagatgct ttacaaaac 
      tatgcagca aaatggatt aaaaagaac gaagagaac 
      atccgtcgt acaataaaa attttgatc aagcacagt 
      ttggacgcg ttgtttgta ttcgattgt aaaattcta 
      atttattta tcctttatt tcgatcaac atggacaca 
      gctataaaa gaaattgaa gaaaaagaa gctagtcct 
      tctgaatct gttgaaacg gaggaattg gacatcgag 
      agcgaacct ttctttcag gatatttac atgctgacc 
      gaacacggc gtccctctt ccagatatc gaagaaatg 
      cgaaaaatt ggcataaac acagtcaag ggcctccaa 
      atgaccaca accgacaaa cttttggct ttgaagagc 
      tttaatccg agtaaagta agcaaaatt caggaaatt 
      tgcggaaat atctctttc agcaatcgc ttcatgacc 
      gcctttgaa gtaagtgag gcatgcaag caagttttt 
      aaaataagt acgggaagc gcaaatttg gataaattg 
      ctgggcggg ggtgtagag tcgatgtct ataactcaa 
      gtttttgga gaagctgga agcggaaaa acccaaatt 
      gcccacact ctatgtgtc accacgcaa ataccgact 
      gaagactat tccgggggc aaagttatg ttcatagac 
      acggagcgt agtttccgc ccaaatcgc attcgccaa 
      attgcacgt cgttttcat ttgagtgaa gacagtgtc 
      ttgcagaat attctctac attagagct tataactct 
      gaacatcaa tatcagatc ctcaaaaat gttgctgta 
      aagtttcat gaagatacg ggcgtgttt aagcttctt 
      attgtcgac tctattata gcattattc cggaatgat 
      tttatgggc agaggtgtg cttctgaat aggcagcaa 
      aaattggcg gaaacaatg tcgcttttg aaaaaaatc 
      agtgaggaa tataatgtt gctgttttc ataacaaat 
      caagttacg acaagtatg aataataaa ttcccgttg 
      ttgactatc ggtgatgat gttaagcct gtcggaggg 
      aatattttg gcccattcg tctactact agagtggct 
      ttgagaaag cttacagga aatgtgagg attgccaag 
      atccacgat agtcctgag cttgctgaa caagaagaa 
      gcttttact ataactaat ggaggagta caagaccct 
      atattagat gcggaataa attactata atttaagag 
      gaaaaaaag ttaagttta gtaagaata ttaatttat 
      tttgagtgt tgaatagca tgtctgaaa ataaagcaa 
      agtcgattt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY