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Gene information
General information
TC003144 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1171704..1190195
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003144
    iBeetle screen iB_03505

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003144 RA
      MGKKNSKLK QDTIDRLTT ATYFTEKEI 
RQWHKGFLK DCPNGLLTE QGFIKIYKQ 
FFPQGDPSK FASLVFRVF DENNDGSIE 
FEEFIRALS VTSRGNLDE KLHWAFRLY 
DVDNDGFIT RDEMYNIVD AIYQMVGQQ 
PSEDENTPQ KRVDKIFDQ MDKNHDDRL 
TLEEFREGS KADPRIVQA LTLGPE
    CDS sequence
    • >TC003144 RA
      atgggtaaa aaaaattcc aagctaaag caagacaca 
atcgacaga ctcaccacc gccacatac tttacagaa 
aaggaaata aggcaatgg cataaagga tttttgaag 
gactgtccc aacggtctt ctaacagag cagggcttt 
ataaaaatt tataaacaa ttcttccca caaggagat 
ccttccaaa tttgcctct ttagtgttt agagttttc 
gacgaaaat aacgacggt tctatcgag ttcgaggaa 
tttattcga gcactctcg gttacgtct agaggcaat 
ctggacgaa aaattacat tgggcattt agactttat 
gatgtggac aacgacggt ttcataaca agagacgaa 
atgtacaat attgtggac gccatttat caaatggtg 
ggccagcag ccgtccgag gatgaaaac actccgcag 
aaacgagtg gacaagatc ttcgatcaa atggataaa 
aatcacgac gaccggcta accctggaa gagttccgg 
gaaggcagc aaagccgac ccgagaatt gtccaggct 
ttaacgctg ggtcctgaa tag
    mRNA sequence
    • >TC003144 RA
      gttaactgc aggatgtgc gacagtgaa cttcgtagg 
gtaaaggcg caagcgtaa cttctatct accctgtac 
agtaggagt ttgagtgtt accctttag tattgtgcg 
atcggtaga aattcgtac gttgttctt gtaagttgg 
aaacgaaac actccaaca tgggtaaaa aaaattcca 
agctaaagc aagacacaa tcgacagac tcaccaccg 
ccacatact ttacagaaa aggaaataa ggcaatggc 
ataaaggat ttttgaagg actgtccca acggtcttc 
taacagagc agggcttta taaaaattt ataaacaat 
tcttcccac aaggagatc cttccaaat ttgcctctt 
tagtgttta gagttttcg acgaaaata acgacggtt 
ctatcgagt tcgaggaat ttattcgag cactctcgg 
ttacgtcta gaggcaatc tggacgaaa aattacatt 
gggcattta gactttatg atgtggaca acgacggtt 
tcataacaa gagacgaaa tgtacaata ttgtggacg 
ccatttatc aaatggtgg gccagcagc cgtccgagg 
atgaaaaca ctccgcaga aacgagtgg acaagatct 
tcgatcaaa tggataaaa atcacgacg accggctaa 
ccctggaag agttccggg aaggcagca aagccgacc 
cgagaattg tccaggctt taacgctgg gtcctgaat 
agcataagg atgcgggaa ttttacccg acacactct 
gacgcaagc aggggacgt tattcgaaa ccaacaatt 
tcaaatacg aatccgact tttacaaaa ttctctcgt 
cttattgtt attaagacg gggcagttt ttggctgtt 
ttttacatg tcgaatttt gtccctcgt gtcaaaact 
acggctacg acgctcaat tataaatat tagggagtt 
gaataaaac gtctttatt atagttctg ttttgttca 
agttagggt gtaaaaaga cggttttat tacgtggcg 
ttacaaatt ataccgaca gacaatcat cagatttct 
ttgctgtgc tcttttact tgcatgact ttcaacttc 
atatcaaaa cgatttttt tagttgact tattttcct 
aacagaact tcagctctt gtttgtgtg cactttatt 
caaagtaaa acaagtgtc aaataaaaa taagtaagc 
taaaaagta aattaatac atatgtcta taaacacca 
tactggagg aaattttat acatatttc catgttagc 
aatatcctt tttacgtcc aggtttggg gttttaaaa 
gcgtcactg ttaaaaata tttgcatat aaagtgatg 
ttttaaact actggatgt attttccct caactccat 
agaagcaat aaaaaaaat aaatggtaa ttcactgca 
tagtaaaat taatacgat ataagctca ataagtgca 
tttatttat gtccagtaa tttaaagtt atcactgtt 
cgaattact ctttacgac actgcatgt atgatgaac 
gataaaagt tatcacaaa ttttattcc attttctct 
ctacctaaa ctatgatac ttataacat gtagtaata 
gattattaa tggtagttt taacctata catgtacta 
taatcattt atcgactat tttaaacta aagacaaaa 
cacttaatg ccgaaaagc aaatcttaa atctgtatt 
tttatgaac aaaaatttg ttaaaatat ttgtgccta 
tttatttta taaagcttt tgcaacgtt gtaaatgtt 
caaaacgag ttgtacgtt acttttctg tattgttat 
caaaaaatc gaatggtaa aaaactgca aaaatcggt 
atgacaata cccatcaat aatgataag caatttgaa 
ataactgtt aggattttt ttaaagaca actaagaaa 
tagtcgttg tcgatacgg tttatacaa tttaagtat 
tcactattc ttccttgta gttgtactt ttatgtaac 
aaacattaa agtgatagt tggcagtgt tagttgtta 
actaattct aaaattttt tagctatgc catgcacac 
ttgttgagt gattttcag ttaccacat tgtagaaga 
aataatgtg attgcgcgt tgttaaatt tttgattat 
ttgtaataa tttaatagc gcggcctaa cagtatgat 
gcatttaaa ttgttgtta taagtttac gttttgact 
gtagcgacg cgctatttt tttttgtta acaaccttc 
tgtgataat tacaatcat tgtatcgca ataatgaaa 
atgctgtgt cttgtattg ccgtaggct gttcttgtt 
gttttagat aattcgtaa catgattgt tttttgaca 
ctgtttgtt gttgtaaat tatatgaag caaatacat 
gacaactgt gttttgtaa tattaaaac taagtgtac 
aaatatgtg gcataaatt acaataata aaatcatta 
ataacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY