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Gene information
General information
TC003140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1221940..1223685
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003140
    iBeetle screen iB_03504

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003140 RA
      MKNGLLDTY AAWTAYRKC SSQSSGASQ TDCYEDDIK 
      EKILAASLP FVVELGWSR SALGAGAQA VGYPGVTHG 
      LFPRGGADL VLYFQRTSN LKLVEILKE METKQRESP 
      MPPAVFVEK ALQSRLQMI IPYLSRWPQ AIAIMSLPP 
      NVPNALATI LTAVDDICY YAGDRSVDF NWYARRLGV 
      AGVYKATEL YLIQDTSPE YEATWKFLN RRLAEAVQI 
      HETLCKMDL GSAGPKDAV TSAFVTARN ILGLNWSR
    CDS sequence
    • >TC003140 RA
      atgaaaaac ggtttatta gatacgtac gctgcttgg 
      acggcatat aggaagtgc tcttcccag tcatcgggc 
      gcgagccaa accgactgc tacgaagac gacatcaag 
      gaaaaaatt cttgccgct tccttgccc tttgtagtc 
      gaactggga tggtctcgg agcgcgctg ggcgctggc 
      gcccaagct gtgggctat ccgggagtc acccatggg 
      ctgtttcct cgcggaggc gccgaccta gtcctctac 
      ttccaaaga acttcgaac ttgaagtta gtggaaatc 
      ctcaaagag atggaaacc aaacagcgc gagtcgccg 
      atgccgcca gccgttttc gtcgaaaag gcgctccaa 
      agccgcctc cagatgatt attccctac cttagccgg 
      tggccgcag gcgatagcc ataatgtcg ctgccgccg 
      aatgtgccc aacgctttg gcgacgatt ttgactgcg 
      gtcgatgat atttgttac tacgccggt gaccggtcg 
      gtagatttc aactggtat gccagaaga ttaggggtg 
      gctggggtg tataaagcg actgaattg tacctgatt 
      caagacact tcacctgag tatgaagct acgtggaag 
      ttcctaaat aggaggttg gctgaggcg gtgcaaatt 
      cacgaaact ttgtgcaaa atggatttg ggcagtgcg 
      gggcctaag gacgcagtc acgtcggct tttgtaacg 
      gcgaggaat attctaggg ttgaactgg agtaggtag
    mRNA sequence
    • >TC003140 RA
      cctagttat tgtaacaaa atgaaaaac ggtttatta 
      gatacgtac gctgcttgg acggcatat aggaagtgc 
      tcttcccag tcatcgggc gcgagccaa accgactgc 
      tacgaagac gacatcaag gaaaaaatt cttgccgct 
      tccttgccc tttgtagtc gaactggga tggtctcgg 
      agcgcgctg ggcgctggc gcccaagct gtgggctat 
      ccgggagtc acccatggg ctgtttcct cgcggaggc 
      gccgaccta gtcctctac ttccaaaga acttcgaac 
      ttgaagtta gtggaaatc ctcaaagag atggaaacc 
      aaacagcgc gagtcgccg atgccgcca gccgttttc 
      gtcgaaaag gcgctccaa agccgcctc cagatgatt 
      attccctac cttagccgg tggccgcag gcgatagcc 
      ataatgtcg ctgccgccg aatgtgccc aacgctttg 
      gcgacgatt ttgactgcg gtcgatgat atttgttac 
      tacgccggt gaccggtcg gtagatttc aactggtat 
      gccagaaga ttaggggtg gctggggtg tataaagcg 
      actgaattg tacctgatt caagacact tcacctgag 
      tatgaagct acgtggaag ttcctaaat aggaggttg 
      gctgaggcg gtgcaaatt cacgaaact ttgtgcaaa 
      atggatttg ggcagtgcg gggcctaag gacgcagtc 
      acgtcggct tttgtaacg gcgaggaat attctaggg 
      ttgaactgg agtaggtag tatttcaat ttagctaag 
      taattaatt gttccaatt attgttgtt ggtatctgt 
      gaggtagtt ttatataaa aattacaac tccaagtta 
      tttgctgtt ttatttata tttattaca aatttaaat 
      atcttagtt aactctata acaaatatt ttgactcaa 
      taatcatgt attttcagt tcacgtatt actgcgtga 
      ttcgcaaaa taccaaaat agaattata taatgtttt 
      acaaaacta acatgtatt tttctctct aaatagacg 
      tggaaagga tattggcga atcatttgg gtaatagga 
      ataacgatt ataaattct tgttcagtc aaataaaaa 
      tttcaaaca aacctaatc attatttaa cacgataat 
      aactaaaat acaattctg tacaagata gtttatgca 
      caaacggtt caaaaataa atctgtaca agttttaac 
      atgttctca atttaataa attttgtta attaccaag 
      ttatagttt tctcaaact atccgacaa tttatcaag 
      gtgattcat tctttaggc ttgataact gcaacttat 
      caacattta atcaatgtt atcaacaag attgttaca 
      tccaaacta gtgtatttc attatttaa aatcagctt 
      tttattgca accgacacc ccaaattac acaactgtg 
      acaatagaa atattctga agttttaaa cgttcaatg 
      ttttattga acttggaca tttgttagt gtccttata 
      acagttctt agcatcgta cgtttaagt tcactacaa 
      tgaattttt aatgtttag ttcacataa aagaaaact 
      atattttta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY